Professional Documents
Culture Documents
Articol Metode de Extractie
Articol Metode de Extractie
The essential oil of Salvia species has various compositions depending on the genetic,
climatic, seasonal, and environmental factors.[16] Some chemical compounds like flavonoids,
terpenoids, and essential oils are present in different species of Salvia. Essential oils are very
important sources for the screening of anticancer, antimicrobial, antioxidant, and free radical
scavenging agents.[17] S. officinalis (common sage) is considered to have the highest amount of
essential oil compared to the other species of Salvia.[5,12]
The major phytochemicals in flowers, leaves, and stem of S. officinalis are well identified. A
wide range of constituents include alkaloids, carbohydrate, fatty acids, glycosidic derivatives
(e.g., cardiac glycosides, flavonoid glycosides, saponins), phenolic compounds (e.g., coumarins,
flavonoids, tannins), poly acetylenes, steroids, terpenes/terpenoids (e.g., monoterpenoids,
diterpenoids, triterpenoids, sesquiterpenoids), and waxes are found in S. officinalis. Structure of
main flavonoids and terpenes/terpenoids isolated from S. officinalis is shown in Fig.4 and Fig. 5,
respectively. Most of the phytochemicals which are reported from S. officinalis have been
isolated from its essential oil, alcoholic extract, aqueous extract, butanol fraction, and infusion
preparation. More than 120 components have been characterized in the essential oil prepared
from aerial parts of S. officinalis. [1]
Fig.4
Fig.5
Compound* S. S.
officinalis**officinalis***
(1R)-(+)-α-Pinene 3.70 4.50
(−)-Camphene 2.60 5.00
β-Pinene 6.00 5.20
Sabinene - 0.30
β-Myrcene 3.00 3.50
α-Terpinene - 0.40
(R)-(+)-Limonene - -
1,8-Cineole 62.0 55.0
γ-Terpinene 0.30 0.50
P-Cymene 0.60 0.60
Terpinolene - 0.20
(−)-α-Thujone 1.38 1.80
β-Thujone 0.72 1.50
Camphor 8.0 10.0
(−)-Linalool 0.80 0.80
Linalyl acetate 0.60 0.30
(−)-Trans-caryophyllene 2.00 1.00
Monoterpene 1.26 1.10
(+)-Menthol - -
Borneol 5.00 4.50
α-Terpineol 0.20 -
Geranyl acetate 0.30 -
Geraniol 0.10 0.25
Phytol 0.18 -
Thymol 0.80 0.70
Carvacrol 0.20 0.40
Farnesol 0.20 -
Trans, trans-Farnesol 0.06 0.15
Total components 45 30
*Compounds of essential oil extracted from fresh green leaves and flowering top; **S. officinalis
L. collected at 100 m above the sea level; ***S. officinalis L. collected at 500 m above the sea
level
3. Pharmacological activities
3.2.Antioxidant activities
Oxidative stress plays an important role in the initiation and progression of several
diseases, such as cancer, cardiovascular disorders, diabetes, and neurological diseases.38
Enhanced oxidative stress occurs when the generation of ROS by mitochondrial electron-
transport chain, NADPH oxidase, uncoupled nitric oxide syntheses, and xanthine oxidase,
exceeds the potential of antioxidant defenses including catalase, glutathione per oxidase, and
superoxide dismutase activities. [39] Natural antioxidants protect cells against ROS over
production and therefore can counteract oxidative stress-mediated tissue damage. Evidence from
several studies suggests that S. officinalis has potent antioxidant activities. Enriching the
drinking water of rats with S. officinalis extract increases resistance of rat hepatocytes against
oxidative stress. [39] It protects hepatocytes against dimethoxy naphthoquinone- and hydrogen
peroxide-induced DNA damage through elevation of glutathione peroxidase activity. [37] The
most effective antioxidant constituents of S. officinalis are carnosol, rosmarinic acid, and
carnosic acid, followed by caffeic acid, rosmanol, rosmadial, genkwanin, and cirsimaritin. [40]
The radical scavenging effect of carnosol is comparable to that of α-tocopherol. [41] The
superoxide scavenging activity of the rosmarinic acid derivatives are 15–20 times more than
trolox, a synthetic water-soluble vitamin E. In streptozotocin-induced diabetic rats, rosmarinic
acid increases activities of pancreatic catalase, glutathione peroxidase, glutathione-S-transferase,
and superoxide dismutase. [42] In addition to rosmarinic acid, other flavonoids of S. officinalis
particularly quercetin and rutin have strong antioxidant activities. [42] For example, in our
previous work we showed that rutin reversed hexachlorobutadiene-induced elevation of lipid
peroxidation and depletion of thiol content in the kidney. [43]
3.4.Antiseptic effects
Several lines of evidence support antimicrobial effects of S. officinalis. The essential oil and
ethanolic extract of S. officinalis show strong bactericidal and bacteriostatic effects against both
Gram-positive and Gram-negative bacteria. Among Gram-positive pathogens, Bacillus cereus,
Bacillus megaterium, Bacillus subtilis, Enterococcus faecalis, Listeria monocytogenes, and
Staphylococcus epidermidis show high sensitivity to S. officinalis. [46] Effects of S. officinalis
on Gram-negative bacteria depend on the type of extract used. While essential oil of
S. officinalis has significant inhibitory effect on the growth of Aeromonashydrophila,
Aeromonassobria, E. coli, Klebsiella oxytoca, Klebsiella pneumonia, Pseudomonas morgani,
Salmonella anatum, Salmonella enteritidis, Salmonella typhi, and Shigellasonei, effect of
ethanolic extract on E. coli, Pseudomonas aeruginosa, and S. enteritidis is weak. [46]
There is increasing evidence to suggest that S. officinalis has cognitive- and memory-
enhancing effects. In animal studies, it has been shown that ethanoic extract of S. officinalis
increases memory retention of passive avoidance learning in rats.48 Hydroalcoholic extract from
S. officinalis and its main flavonoid rosmarinic acid improve cognition in healthy rats and
prevent learning and memory deficits induced by diabetes. [48] Also, S. officinalis hydroalcoholic
extract attenuates morphine-induced memory impairment. [48]
Clinical trials confirm the results of animal studies and demonstrated that S. officinalis
enhances cognitive performance both in healthy participants and patients with cognitive
impairment or dementia. [48] Moss et al reported that the aroma of S. officinalis essential oil could
enhance prospective memory performance in healthy adults. [48] Also, Scholey et al showed that
ethanolic extract of this plant improved memory and attention in healthy older subjects. [48] A
randomized controlled trial by Akhondzadeh et al showed that a 4-month treatment with
hydroalcoholic extract of S. officinalis improved cognitive functions in patients with mild to
moderate Alzheimer's disease. [48]
With regards the mechanisms responsible for cognitive- and memory-enhancing effects of
S. officinalis, a potential interaction with cholinergic system has been suggested. Eidi and
coworkers found that activation of muscarinic and nicotinic receptors by pilocarpine and
nicotine, respectively, potentiated memory-enhancing effects of S. officinalis. On the other hand,
blockade of muscarinic and nicotinic receptors by scopolamine and mecamylamine, respectively,
attenuated this effect. In addition, S. officinalis has been reported to inhibit acetylcholinesterase
activity. To date, inhibitors of acetylcholinesterase are the leading therapeutics of Alzheimer's
disease and S. officinalis might be a promising source for developing therapeutic agents for this
disease. [48]
3.6.Metabolic effects
Experimental and clinical studies have confirmed the beneficial effects of some medicinal
plants on body metabolism particularly glycemic status, serum lipids, lipolysis, and
adipogenesis. Recent pharmacological investigations demonstrated that different extracts of
aerial parts of S. officinalis are able to decrease blood glucose in normal and diabetic conditions.
The mechanisms suggested for hypoglycemic effect of S. officinalis include an inhibition of
hepatocyte gluconeogenesis and decrease of insulin resistance through stimulation of peroxisome
proliferator-activated receptor γ (PPARγ). Recently, one study group reported that S. officinalis
extract increased plasma insulin in streptozotocin-induced diabetic rats. However, in their
previous work they observed that the extract did not affect insulin releasing from the pancreas of
normal or diabetic rats. Therefore further studies required to elucidate whether stimulation of
insulin release mediates hypoglycemic effect of S. officinalis. [49]
Pharmacological studies also revealed that different extracts of S. officinalis reduces serum
lipids. Hernandez-Saavedra et al Reported that infusion prepared from this plant reduced serum
triglycerides, total cholesterol, and low density lipoproteins (LDL) levels in diet-induced obese
rats. It also decreased body weight and abdominal fat mass in these animals. The beneficial
effects of S. officinalis on lipid profile have been also shown in diabetic animals. It could
decrease the level of triglyceride, cholesterol, urea, uric acid, creatinine, aspartate amino
transferase (AST), and alanine amino transferase (ALT) in streptozotocin-induced diabetic rats.
In clinical trials, extract of S. officinalis leaf could lower the blood levels of triglyceride, total
cholesterol, LDL, very low density lipoproteins (VLDL) and 2 h postprandial glucose in patients
with hyperlipidemia and diabetes. The beneficial properties of S. officinalis tea consumption on
serum lipid profile have been also reported on non-diabetic healthy volunteers. Because
hyperlipidemia is a common metabolic disorder contributing to mortalities and morbidities due
to cerebrovascular and cardiovascular diseases, S. officinalis may be valuable for the
management of dyslipidemia in high risk patients like those with diabetes mellitus or
hypercholesterinemia. The beneficial action of S. officinalis on dyslipidemia may be related to
flavonoids present in the plant. For example, rosmarinic acid treatment reduces the levels of
triglycerides and cholesterol in serum of high fat diet- and streptozotocin-induced type 2 diabetic
rats. Also, administration of rutin reduces adipose tissue mass and body weight in high-fat diet-
induced obese rats. In addition, this flavonoid increases mitochondrial size, mitochondrial DNA
content, and gene expression related to mitochondrial biogenesis (e.g., PPARγ coactivator-1α,
nuclear respiratory factor-1, transcription factor A, and nicotinamide adenine dinucleotide-
dependent deacetylase) in skeletal muscle. [49]
4. Toxicological studies
A number of clinical trials have reported that consumption of S. officinalis does not
induce severe side effects. [50] However, in the case of prolonged use or following overdose of
ethanolic extract and volatile oil of S. officinalis (corresponding to more than 15 g of the leaves)
some unwanted effects such as vomiting, salivation, tachycardia, vertigo, hot flushes, allergic
reactions, tongue swallowing, cyanosis, and even convulsion may occur. [51] The proconvulsant
action of S. officinalis oil is due to its direct effect (at doses more than 0.5 g/kg) on nervous
system. [50] Camphor, thujone, and terpene ketones are considered as the most toxic compounds
in S. officinalis. These compounds may induce toxic effects on the fetus and newborn. Therefore
consumption of S. officinalis is not recommended in pregnancy and lactation. Results from
animal studies have demonstrated that the LD50 of S. officinalis oil (when consumed orally) and
the methanolic extract (when injected intraperitoneally) is 2.6 g/kg and 4 g/kg, respectively. It
has been reported that S. officinalis tea enhances CCl4-induced hepatotoxicity in mice. However,
in clinical studies no hepatotoxic effects were reported. [51]
5. Cosmetic properties
The cosmetics are the articles intended to be rubbed, poured, sprinkled or sprayed on,
introduced into, or otherwise applied to the human body or any part thereof for cleansing,
beautifying, promoting attractiveness or altering the appearance, and articles intended for use as
a component of any such articles; except that such term shall not include soap.
The main objective of the cosmetic application is decorative to enhance the general appearance
of face and other body parts to minimize the skin defects to a considerable extent. It is applied to
maintain or improve the status of skin and hair. It greatly helps men and women to look more
impressive, beautiful and smart to a considerably extent. Although the aim is not always
achieved depending upon the selection of the quality product based on the type of skin ( oily
skin, dry skin, normal skin and combined skin) and hair and also due to lack of artesian skill. It
requires adequate expertise and artistic skill, which could be achieved through practice.
The other objectives of cosmetic application are Psychological, Social and Clinical.
Psychological effect of cosmetic application is an important value, which yield mental
satisfaction to user. The skillful use of cosmetics can often disguise or minimize the facial and
skin defects and improve the mental level of sufferers towards the life. As regards to clinical
effects of cosmetics, there exist different opinions for action of creams and other preparations on
the skin. Their application generally check the skin cracking, skin wrinkling, pre-mature ageing,
minimizing effects of wind burn, sun burn, bacterial infection, etc.
Herbal cosmetics are the preparations, which represent cosmetics associated with active
bioactive ingredients or pharmaceuticals. The use of phytochemicals from a variety of botanicals
have dual function, they serve as cosmetics for the care of body and its parts and the botanical
ingredients present influence biological functions of skin and provide nutrients necessary for the
healthy skin or hair. In general, botanicals provide different vitamins, antioxidants, various oils,
essential oils, dyes, tannins, alkaloids, carbohydrates, proteins, terpenoids and other bioactive
molecules.[52]
These are also topically applied and considered more preferred with compare to cosmetics.
Personal care industry is now more concentrated on herbal based cosmetics as it is a fast growing
segment with a vast scope of manifold expansion in coming years.
Their exist hundreds of botanicals which contain bioactive phytochemicals for their novel action
to improve the skin conditions to considerable extent without any side effects. No other
ingredient can serve this purpose as well as botanicals. These become now part of every product
in the market form of cosmetics. They are also well-known for their action to control scabies,
itching, skin disorder, pimples, dermatitis, ringworm, skin erruptions, acne, allergic rashes, warts
and other problems. Their antioxidant action has resulted their use in numerous formulations.
There is no doubt there exists hundreds of plants for skin and hair care system, some of which
are not commercially feasible as regard to their easy availability and cost constraints.
Salvia officinalis has been cultivated since ancient times. Greeks used to consider it a panacea.
Before tooth paste was invented, people used to rub fresh sage leaves on their teeth in order to
keep them white and shining. Decoction of 20 g sage leaves for 3 minutes was traditionally used
as a mouth wash to prevent gum-bleeding and tooth decay.
Sage is a popular medicine to treat anxiety, irritability, dyspepsia, hepatobiliary dyskinesia and
menopause-related problems; it is used as an appetizer, to reduce lactation and limbs sweating
and to treat diabetes. Common sage and red sage are used for mouth wash, douche (leucorrhea)
and baths to treat dermatological conditions. It is usually applied on infected wounds, aphthae
and torpid ulcers.[53], [54]
5. 1. Cosmetic product with purifying and antiseptic activity
Differently from sage essential oil, sage tincture prepared from the leaves, showed antibacterial
activity against Staphylococcus aureus and S.pyogens. The antimicrobial activity is mainly due
to the thujones in the essential oil and, to a lesser degree, to other oil components such as
pinenes, camphor, 1,8-cineol, borneol, caryophyllene and its bitter principle picrosalvin. Recent
research evidenced the relevance of rosmarinic acid in sage antimicrobial activity
Sage extract is useful to formulate cosmetic products with purifying and antiseptic activity due to
its antimicrobial activity.
5. 2. Cosmetic products for the protection of skin and hair against oxidative processes
S.officinalis leaves have a well-known antioxidant activity based on their phenolic compounds
content. The phenolic diterpenes showing the strongest antioxidant activity are: carnosic acid,
carnosol, rosmarinic acid, caffeic acid, rosmanol and genkwanin. Thus, sage extract is useful to
formulate cosmetic products for the protection of skin and hair against oxidative processes.[55]
5.3. Cosmetic products with anti-perspirant activity
Externally applied thujones seem to have antihydrotic activity, since they block the nerve
terminals in sweat glands and act on the thermoregulatory center of the bulb, all of which makes
these compounds useful to treat bromhidrosis. An open clinical study with 80 patients suffering
idiopathic bromhidrosis demonstrated the beneficial effects of sage extract (leaves infusion
equivalent to 4.5g/day), during a 4 weeks-treatment, which reduced secretion by a bit less than
50%. A clinical study with 30 menopausal women showed that administration of sage and alfalfa
(Medicago sativa) reduced flushes and nocturnal sweat in 20 of them. In principle, this activity is
attributed to a central anti-dopaminergic action.
Therefore, sage extract is recommendable to formulate cosmetic products with anti-perspirant
activity.
5.4. Cosmetic products with anti-irritant activity
It has been approved the topical use of sage leaves to treat inflammations in the nose and throat
mucosa by the Commision E. ESCOP recommends sage to treat inflammations such as
stomatitis, gingivitis and pharyngitis, as well as hyperhydrosis. The German Standard License
recommends sage leaves infusion to treat inflammations in the mouth and throat mucosa. Thus,
sage extract is useful to formulate cosmetic products with anti-irritant activity.
5.5. Cosmetic products for hair care
S.officnalis is one of the best plants to darken and strengthen the hair. To this end, infusion of
sage leaves is used. Sage is also used to treat alopecia. This plant is a good hair tonic. Used as a
lotion, sage infusion gives shine to the hair. Sage is particularly beneficial to dark hair,
strengthening it and boosting its natural color.
Therefore, sage extract is useful to formulate hair care cosmetic products.
5.6. Cosmetic products with astringent and wound healing activity
S.officinalis astringent activity is due to its tannin content. Tannin-containing products act on the
basis of tannins’ affinity for protein molecules. On topical applications, they coat the outermost
layers of skin and mucosa, thus protecting deeper layers. They also act as vasoconstrictor agents
on the small superficial blood vessels. Since they reduce fluid loss and prevent environmental
damages, tannins promote tissue regeneration in superficial wounds and burns.
Therefore, sage extract is useful to formulate cosmetic products with astringent and wound
healing activity. [56]
The reference publication Plants preparations used as ingredients of cosmetic products. Vol I.
(Council of Europe, 1994) includes a monograph on the dry hydroalcoholic extract, the fluid
aqueous extract and the glycolic extract of S.officinalis leaves, which mentions the following
cosmetic effects and recommended concentrations: tonic, deodorant, purifying, reactivating, anti-
perspirant and anti-dandruff ,up to 1% dry extract , up to 5% glycolic and fluid extracts, when
used in stimulating and purifying hair lotions, tooth pastes, greasy and opaque skin products,
deodorants and anti-perspirants. , up to 10% in bath and shower gel and shampoo.
5.7. Cosmetic applications
Salvia, this fresh, bitter, but slightly spicy flavor plant has been used for hundreds
ofyears in Italian cuisine, but has been borrowed over time by chefs around the world.
This spice that is part of the mente family can be used in a variety of ways, not just for
the preparation of tea, poultry or stuffing. It supports warmth well, so it should not be added to
the end just like other flavored herbs. If you have sage in pots, find out that the inflorescence can
sprinkle over salads or make jelly or syrup from it. Vegetable sausages are even more flavourful
with a little sage, and the combination of sage, rosemary and thyme will anonymously remove
any preparation.
Use it carefully because it can dominate the flavor of the rest of the ingredients. Use
salvia for tomatoes, potatoes, green vegetables, minced meat.
7. Precautions and contraindications
You have to be very careful when you start a sage cake. This plant is contraindicated to
epileptics, breastfeeding mothers, pregnant women, stomach susceptible persons. Also, sage
volatile oil is not given to children under the age of 10, and diabetics should keep blood sugar
under close supervision if they start a sage cure.
That means the uses for Sage essential oil may be helpful as long as you pay attention
and respect a few safety guidelines:
Never use Sage essential oil undiluted! Be safe and mix the essential oil with
any vegetable oil you have.
Don’t even think to take Sage oil internally. Unless, of course, you’ve
consulted a doctor first.
Sage essential oil is not recommended to people who take anticoagulants,
because it contains vitamin K. Sage oil may also interact with anti-depressant
medications.
Never go above the recommended dose. Go to the doctor the moment you
experience any nausea, vomiting, disorientation or convulsions.
Volatile oils are never used internally, they are only used for external
application.Before using volatile oils, perform a test on a small piece of skin with a diluted
amount.
[3] - Smidling, D.; Mitic‑Culafic, D.; Vukovic‑Gacic, B.; Simic, D.; Knezevic‑Vukcevic, J.;
Evaluation of antiviral activity of fractionated extracts of Sage Salvia L (Lamiaceae) 2008
[4] - Chipault, J.; Mizumo, G.; Lundberg, W.; The antioxidant properties of spices in food 1956
[5] - Farag, R.S.; Hewedi, F.M.; Antioxidant activity of some spice essential oils and linoleic
acid oxidation on aqueous media 1989
[6] - Lamaison, J.L.; Petitjean-Feytet, C.; Carnat, A.; Teneurs en acide rosmarinique, en derives
hydroxycinnamiques totaux et activite antioxydante chez les Apiacees 1990
[7] - Schwartz, K.; Antioxidative constituents of Rosmarinus Officinalis and Salvia Officinalis
1992
[8] - Cuvelier, M.E.; Berset, C.; Richard, H.; Antioxidant constituents in sage (Salvia officinalis)
1994
[9] - Hohmann, J.; Zupko, I.; Redei, D.; Protective effects of the aerial parts of Salvia
Officinalis, Melisa Officinais and Lavandula angustifolia and their constituents against enzyme-
dependent and enzyme-independent lipid peroxidation 1999
[10] - Baricevic, D.; Bartol, T.; The biological/pharmacological activity of Salvia genus 2000
[11] - Aruoma, O.L.; Free radicals, oxidative stress and antioxidants in human health and
disease 1998
[12] - Rami, K.; Li, Z.; Antimicrobial activity of essential oil of Salvia of. L. collected in Syria
2011
[13] - Tinzoh, S.; Zimmerman, L.; Stuhlinger, B.; Lachenmeier, W.; Antioxidant capacity and
polyphenolic composition as quality indicators for aqueous infusions of Salvia officinalis 2011
[14] - Loizzo, M.R.; Tundis, R.; Menichini, F.; Saab, A.M.; Statti, G.; Menichini, F.; Cytotoxic
activity of essential oils from Labiatae and Lauraceae families against in vitro human tumor
models 2007
[15] - Capillary gas chromatography-mass spectrometry of volatile and semi-volatile compounds
of Salvia officinalis, V. Radulescu, S. Chilment, E. Oprea, 2004
[16] - Hadri, A.; Gomez Del Rio, M.; Sanz, J.; Coloma, A.; Idaomar, M.; Ozanas, B.; Cytotoxic
activity of α-humulene and transcaryo‑phyllene from Salvia officinalis in animal and human
tumor cells 2010
[17] - Hussain, A.; Anwar, F.; Iqbal, T.; Bhatti, I.; Antioxidant attributes of four Lamiaceae
essential oils 2011
[18] - Akhondzadeh, S.; Noroozian, M.; Mohammadi, M.; Ohadinia, S.; Jamshidi, A.H.; Khani,
M.; Salvia officinalis extract in the treatment of patients with mild to moderate Alzheimer's
disease: a double blind, randomized and placebo-controlled trial 2003, 53-59
[19] - Kennedy, D.O.; Pace,S.; Haskell, C.; Okello, E.J.; Milne, A.; Scholey, A.B.; Effects of
cholinesterase inhibiting sage (Salvia officinalis) on mood, anxiety and performance on a
psychological stressor battery Neuropsychopharmacology 2006, 3, 845-852
[20] - Scholey, A.B.; Tildesley, N.T.; Ballard, C.G.; .An extract of Salvia (sage) with
anticholinesterase properties improves memory and attention in healthy older volunteers
Psychopharmacology 2008, 198, 127-139
[21] - Hubbert, M.; Sievers, H.; Lehnfeld, R.; Kehrl, W.; Efficacy and tolerability of a spray
with Salvia officinalis in the treatment of acute pharyngitis – a randomised, double-blind,
placebo-controlled study with adaptive design and interim analysis 2006, 11, 20-26
[22] - Lalićević, S.; Djordjević, I.; Comparison of benzydamine hydrochloride and Salvia
officinalis as an adjuvant local treatment to systemic nonsteroidal anti-inflammatory drug in
controlling pain after tonsillectomy, adenoidectomy, or both: an open-label, single-blind,
randomized clinical trial 2004, 65, 360-372
[23]- Kianbakht, S.; Abasi, B.; Perham, M.; Hashem, F.; Antihyperlipidemic effects of Salvia
officinalis L. leaf extract in patients with hyperlipidemia: a randomized double-blind placebo-
controlled clinical trial 2011, 25, 1849-1853
[24] - Kianbakht, S.; Dabaghian, F.H.; Improved glycemic control and lipid profile in
hyperlipidemic type 2 diabetic patients consuming Salvia officinalis L. leaf extract: a
randomized placebo controlled clinical trial 2013, 21, 441-446
[25] - Behradmanesh, S.; Derees, F.; Rafieian, M.; Effect of Salvia officinalis on diabetic patients
2013, 2, 51-54
[26] - Xavier, C.P.; Lima, C.F.; Fernandes-Ferreira, M.; Pereira-Wilson, C.; Salvia fruticosa,
Salvia officinalis, and rosmarinic acid induce apoptosis and inhibit proliferation of human
colorectal cell lines: the role in MAPK/ERK pathway Nutr Cancer 2009, 61, 564-571
[27] - Pedro, D.F.; Ramos,A.A.; Lima, C.F.; Baltazar, F.; Pereira-Wilson, C.; Colon cancer
chemoprevention by sage tea drinking: decreased DNA damage and cell proliferation 2016, 30,
298-305
[28] - Jedinák, A.; Mucková, M.; Kost'álová, D.; Maliar, T.; Masterova, I.; Antiprotease and
antimetastatic activity of ursolic acid isolated from Salvia 2006, 61, 777-782
[29] – Hadri, A.; Sanz, J.; Cytotoxic activity of α-humulene and transcaryophyllene from Salvia
officinalis in animal and human tumor cells 2010, 76, 343-356
[30] - Oliveira, P.F.; Munari, C.; Nicolella, H.D.; Veneziani, R.C.; Salvia officinalis diterpene,
induces selective cytotoxicity in cancer cells Cytotechnology 2016, 68, 2139-2143
[31] - Jedinák, A.; Mucková, M.; Kost'álová, D.; Maliar, T.; Masterova, I.; Antiprotease and
antimetastatic activity of ursolic acid isolated from Salvia 2006, 61, 777-782
[32] - Xavier, C.P.; Lima, C.F.; Fernandes-Ferreira, M.; Pereira-Wilson, C.; Salvia fruticosa,
Salvia officinalis, and rosmarinic acid induce apoptosis and inhibit proliferation of human
colorectal cell lines 2009, 61, 564-571
[33] - Sharmila, R.; Manoharan, S.; Anti-tumor activity of rosmarinic acid in 7,12-
dimethylbenz(a)anthracene (DMBA) induced skin carcinogenesis in Swiss albino mice 2012, 50,
187-194
[34] - Scheckel, K.A.; Degner, S.C.; Romagnolo, D.F.; Rosmarinic acid antagonizes activator
protein-1-dependent activation of cyclooxygenase-2 expression in human cancer and
nonmalignant cell lines 2008, 138, 2098-2105
[35] - Huang, S.S.; Zheng, R.L.; Rosmarinic acid inhibits angiogenesis and its mechanism of
action in vitro Cancer Lett 2006, 239, 271-280
[36] - Vuković-Gačić, B.; Nikčević, S.; Berić-Bjedov, T.; Knežević-Vukčević, J.; Simić, D.;
Antimutagenic effect of essential oil of sage (Salvia officinalis L.) and its monoterpenes against
UV-induced mutations in Escherichia coli and Saccharomyces cerevisiae 2006, 44, 1730-1738
[37] - Kozics, K.; Klusová, V.; Srančíková, A.; Effects of Salvia officinalis and Thymus vulgaris
on oxidant-induced DNA damage and antioxidant status in HepG2 cells 2013, 141, 2198-2206
[38] - Li, H.; Horke, S.; Forstermann, U.; Oxidative stress in vascular disease and its
pharmacological prevention 2013, 34, 313-319
[39] - Horváthová, E.; Srančíková, A.; Regendová-Sedláčková, E.; Enriching the drinking water
of rats with extracts of Salvia officinalis and Thymus vulgaris increases their resistance to
oxidative stress Mutagenesis, 2016, 31, 51-59
[40] - Cuvelier, M.E.; Richard,H.; Berset, C.; Antioxidative activity and phenolic composition of
pilot-plant and commercial extracts of sage and rosemary 1996, 73, 645-652
[41] - Miura, K.; Kikuzaki, H.; Nakatani, N.; Antioxidant activity of chemical components from
sage (Salvia officinalis L.) and thyme (Thymus vulgaris L.) measured by the oil stability index
method 2002, 50, 1845-1851
[42] - Govindaraj, J.; Sorimuthu, S.; Rosmarinic acid modulates the antioxidant status and
protects pancreatic tissues from glucolipotoxicity mediated oxidative stress in high-fat diet:
streptozotocin-induced diabetic rats 2015, 404, 143-159
[43] - Azevedo, M.I.; Pereira, A.F.; Nogueira, R.B.; The antioxidant effects of the flavonoids
rutin and quercetin inhibit oxaliplatin-induced chronic painful peripheral neuropathy 2013, 9,
53
[44] - Mansourabadi, A.M.; Sadeghi, H.M.; Razavi, N.; Rezvani, E.; Anti-inflammatory and
analgesic properties of salvigenin, Salvia officinalis flavonoid extracted 2015, 1, 31-41
[45] - Pra, V.D.; Bisol, L.B.; Detoni, S.; Denti, M.; Grando, J.; Anti-inflammatory activity of
fractionated extracts of Salvia officinalis 2011, 1, 67-71
[46] - Mitic-Culafic, D.; Vukovic-Gacic, B.; Knezevic-Vukcevic, J.; Stankovic, S.; Simic, D.;
Comparative study on the antibacterial activity of volatiles from sage (Salvia officinalis L.)
2005, 57, 173-178
[47] - Carta, C.; Moretti, M.D.L.; Peana, A.T.; Activity of the oil of Salvia officinalis 1996, 8,
399-404
[48] - Miroddi, M.; Navarra, M.; Quattropani, M.C.; Calapai, F.; Gangemi, S.; Calapai, B.;
Systematic review of clinical trials assessing pharmacological properties of Salvia species on
memory, cognitive impairment and Alzheimer's disease 2014, 20, 485-495
[49] - S. Kianbakht, B. Abasi, M. Perham, F. Hashem DabaghianAntihyperlipidemic effects of
Salvia officinalis L. leaf extract in patients with hyperlipidemia: a randomized double-blind
placebo-controlled clinical trial Phytother Res, 25 (2011), pp. 1849-1853
[50] - Mills, S.; Bone, K.; The Essential Guide to Herbal Safety Elsevier 2005, 558-559
[51] - Halicioglu, O.; Astarcioglu, G.; Yaprak, I.; Aydinlioglu, H.; Toxicity of Salvia officinalis
in a newborn and a child: an alarming report 2011, 45, 259-260
[53] - Deans S.G. & Simpson E.J.M. Antioxidants from Salvia officinalis. En: The Genus Salvia.,
2000, 185-192
[54] - Dweck A.C., The Folklore and Cosmetic Use of Various Salvia Species. En: The Genus
Salvia., 2000, 1-25
[55] - Lu, Y & Foo, L.Y. Antioxidant activities of polyphenols from sage (Salvia officinalis).
Food Chemistry, 2001, 197-202
[56] - Miladinovic D. & Miladinovic L.J. Antimicrobial activity of essential oil of sage from
Serbia. Physics, Chemistry and Technology, 2000, 97-100.
[57] - Merica E., ”Tehnologia produselor cosmetice”, vol.1, Ed. Corson, Iasi, 2000, 172-179.