Leica M50-M60-M80 Manual en
Leica M50-M60-M80 Manual en
Leica M50-M60-M80 Manual en
Leica M60
Leica M80
User Manual
General Instructions
•
This symbol indicates especially impor- This symbol warns against touching hot
tant information that must be read and surfaces, e.g. those of light bulbs.
complied with.
★★ Hazards to personnel This symbol indicates additional informa-
★★ Functional disturbances or damaged tion or explanations that are intended to
instruments provide clarity.
This symbol indicates especially impor- ★★ This symbol within the text stands for addi-
tant information that is mandatory to tional information and explanations.
read and observe.
Figures
Failure to comply can cause the following: (1) Numbers in parentheses within the
★★ Hazards to personnel descriptions relate to the figures and the
★★ Functional disturbances or damaged items within those figures.
instruments
Repairs, service work Installation in third-party products assignments and work flow (changing tasks
★★ Refer to "Safety Concept" booklet ★★ Refer to "Safety Concept" booklet frequently), thorough training of the personnel,
giving consideration to ergonomic and organi-
★★ Only original Leica Microsystems spare Disposal zational aspects.
parts may be used. ★★ Refer to "Safety Concept" booklet The ergonomic design and construction of the
Leica M stereomicroscopy series are intended to
★★ Before opening the instruments, switch off Legal requirements reduce the exertion of the user to a minimum.
the power and unplug the power cable. ★★ Refer to "Safety Concept" booklet
Direct contact with eyepieces can be a potential
★★ Touching the live circuit can cause injury. EC Declaration of Conformity transmission path for bacterial and viral infec-
★★ Refer to "Safety Concept" booklet tions of the eye.
Objectives and
Optical Accessories
The Different Types of Objectives 48
System Illumination
Leica LED3000 NVI™ (Near Vertical Illumination) 50
Leica LED5000 CXI (Coax Illumination) 51
Dimensional Drawings
Leica M50 53
Leica M50 54
Leica M50 55
Leica M60 56
Leica M80 57
Leica M80 58
Leica M80 59
Leica M80 60
Leica M80 61
The New Leica M Series Common to all microscopes is the Leica range of accessories. Whether you
The introduction of the Leica M50, M60 and M80 adds three new high- need a variety of illumination types, a wide selection of objectives or the
quality routine stereomicroscopes to the proven M-Series range from Leica swinging-arm system – there is a solution for everything there!
Leica Microsystems. The optical brilliance and the wide range of acces-
sories make them the ideal microscope for quality assurance and similar Do you already own stereomicroscopic equipment and are thinking of
industrial applications. switching to Leica? No problem! Leica M50, M60 and M80 fit into micro-
scope carrier with a diameter of 76 mm and are therefore compatible with
many suppliers. You can simply continue to use your components and add
Magnification in stages or zoom the proven Leica stereomicroscopes.
The Leica M50 stereomicroscope includes precisely reproducible magni-
fication levels for repeated examinations, measurements, drawing or
photography of objects under absolutely identical conditions. The five
easily selectable positions can be set without removing the eyes from the
eyepieces. This ensures that your results remain comparable at all times
without great efforts.
The Leica M60 and M80 stereomicroscopes can be used for a wide range of
routine applications with the 6:1 / 8:1 zoom and switchable ratchet steps.
The large working distance and the brilliant imaging power shows you
the finest details of your specimens without losing your overview of large
workpieces.
The Leica M series provides maximum flexibil- Despite this, you will notice that the controls specific solutions. If you have a special request
ity in choosing equipment, thanks primarily to and individual components do not differ signif- that cannot be met with standard parts, contact
the modular configuration and the compatibil- icantly. Whichever configuration you choose, your Leica consultant. We have a solution for
ity that Leica has painstakingly maintained for you will quickly feel right at home. every problem.
decades. The optics carriers, eyepieces, bases
and more can be combined in any way you
choose, allowing you to create the microscope Have a special request? Let us know!
that best suits your needs. Leica Microsystems enjoys an exceptional repu-
tation when it comes to devising customer-
If your new Leica microscope has already been If, on the other hand, you are assembling the
assembled and commissioned by your Leica microscope yourself, continue with the "Assem-
consultant, click here to skip through the instal- bly" chapter, which begins on Page 16.
lation instructions and go directly to the Quick
Start Guide on Page 29.
The first step is to connect the focusing column Assembling the column adapter 2. Install the base on the column using the
of the M series to the corresponding base. 1. Remove the three screws provided from the three screws.
column.
Tools used
★★ Hex socket screwdriver, 3 mm
Assembly
The microscope carrier is fastened to the column
using the screw provided.
Tools used Assembling the optics carrier 2. Tighten the clamping screw below the
★★ None 1. Unscrew the clamping screw on the holder optics carrier.
for the optics carrier.
All intermediate tubes that fit between the Assembling the tube
optics carrier and the binocular tube are fitted 2. Push the tube (for example, the inclined 3. While holding the tube only slightly, care-
in the same manner. binocular tube) into the dovetail ring and fully tighten the positioning screw. It
rotate it slightly in both directions until the is automatically brought to the correct
Tools used positioning screw meshes with the guide position.
★★ No tools required. groove.
Preparation
1. Unscrew the positioning screw and remove
the protective cover.
•
★★ No tools required. Hold the objective firmly during assem-
bly and disassembly so that it does not
fall onto the stage plate. This applies particu-
Preparation larly to the 2x planapochromatic objective,
1. Remove the protective cap on the optics which is very heavy. Remove all specimens from
carrier by turning it. the stage plate first.
Required tools Installing the Leica LED5000 CXI 2. Unscrew the positioning screw and remove
★★ None 1. Connect the Leica LED5000 CXI to the the protective cover.
focusing column via the CTL2 connection if
the focusing column is equipped with inte-
grated electronics.
3. Place the Leica LED5000 CXI on the optics 4. Push the tube (for example, the inclined 5. While holding the tube only slightly, care-
carrier and tighten the positioning screw. binocular tube) into the dovetail ring and fully tighten the positioning screw. It
rotate it slightly in both directions until the is automatically brought to the correct
positioning screw meshes with the guide position.
groove.
Required tools Installing the Leica LED3000 NVI™ Alternative installation for manual columns
★★ None 1. Connect the Leica LED3000 NVI™ to the 1. Connect the external power supply unit
focusing column via the CTL2 connection if (10 450 266) to the Leica LED3000 NVI™.
the focusing column is equipped with inte-
The interpupillary distance is correctly set if you Adjusting the interpupillary distance 3. Slowly approach the eyepieces with your
see a single circular image field when looking 1. Look into the eyepieces. eyes until you can see the complete image
at a specimen. field without corner cutting.
2. Hold the eyepieces with both hands. Push
If you are still a novice microscope user, you the eyepieces together or separate them
may need a short time to become accustomed until you see a circular image.
to this. Not to worry—after a little while, it will
become automatic.
Reference value
The distance between eye and eyepiece
measures approx. 22 mm for 10/23B wide-field
eyepieces for persons wearing glasses.
✗ ✓
The eyepieces form the connection between If you do not wear glasses: If you wear glasses:
the tube and the eye of the observer. Simply 1. Hold the eyepiece firmly and rotate the 1. Hold the eyepiece firmly and rotate the
push them into the tube and they are ready to eyecups forwards counterclockwise. eyecups counterclockwise towards the rear,
use. as otherwise the viewing distance is too
great.
Each eyepiece offers a certain magnification
factor that has a determinative effect on the
total magnification. Furthermore, all Leica
eyepieces can be equipped with practical grati-
cules that enable measuring and quantifying of
specimens.
2. If an eyepiece is equipped with the inte-
Dioptric correction grated dioptric correction, turn the value to
A built-in dioptric correction is available for the "0" mark. 2. If an eyepiece is equipped with the inte-
eyeglass wearers. For more information, refer grated dioptric correction, turn the value to
to page 39. the "0" mark.
• load of up to 15 kg.
The coarse/fine adjustment carries a
ment is 1 µm.
The resolution of the coarse/fine adjust-
The zoom button can optionally be operated Enabling and disabling ratchet steps Magnifications and fields of view
either with or without ratchet steps. Continu- 1. Push the button towards the knob to enable The formula on page 62 provide additional
ous zoom is possible when the ratchet steps are the ratchet steps. information about the magnifications and field
disabled, which many users find convenient. of view diameters, with consideration given to
On the other hand, when the ratchet steps are 2. Push the button away from the knob to the position of the magnification changer and
enabled, photographs, measurement results disable the ratchet steps. the eyepiece and objective combination used.
etc. can be reproduced more accurately.
An eyepiece not only makes it possible to look passively into the micro-
scope, but also has a critical effect on the maximum magnification. The
magnification factor is between 10x and 40x.
•
Direct contact with eyepieces is a
potential transmission method for
bacterial and viral infections of the eye. The risk
can be kept to a minimum by using individual
eyepieces or detachable eyecups. Eyecups can
be ordered separately. Please contact your
Leica partner.
All Leica eyepieces are also available with built- Using the Dioptric Correction
in dioptric correction, allowing the microscope 1. Set the dioptric correction of both eyepieces
to be used without glasses even by those with to the mid position ("0" diopter settings).
vision problems. The correction comprises
±5 diopter settings. 2. While wearing your glasses, look through
the eyepieces and focus on the specimen.
Leica stereomicroscopes are parfocally matched. Adjusting 7. Look into the eyepieces.
The prerequisite for this is the correct setting of 1. Set the dioptric correction for both
the diopters and the parfocality. The following eyepieces to "0". 8. Slowly rotate each eyepiece individually
adjustments only have to be carried out once in the "–" direction until each eye sees the
by each user. 2. Select the lowest magnification and focus object sharply imaged.
on a flat specimen.
9. Select the highest magnification and refo-
Preparation 3. Select the highest magnification and read- cus if necessary.
★★ Move the lever of the video/phototube to just the sharpness.
the "observation" position and open the Now, if you adjust the magnification from the
diaphragm. 4. Select the lowest magnification again, but lowest to the highest level, the specimen is
do not look into the eyepieces. always brought into sharp focus. If not, repeat
the process.
6. Rotate the eyepieces counterclockwise
in the "+" direction as far as they will go
(+5 diopter settings).
Use 2. Clamp the graticule on the insert, applying 4. You can now align the graticule by rotating
Leica graticules make length measurements moderate pressure. Ensure that the grati- the eyepiece in the tube and then tighten-
and counting easier, particularly for work- cule fits tightly. ing it using the clamping screw.
stations that are not equipped with a digital
camera and LAS software.
For most microscope users, digital documen- Leica DFC cameras Leica Application Suite
tation has become an invaluable part of their However, if you require absolute control The "Leica Application Suite", or "LAS" for short,
work. Research results can be presented in an over the camera and need the capability for is, as it were, the digital extension of the Leica
attractive manner; measurements on the digi- measurement, evaluation and more in addition M series microscopes. In addition to capturing
tal image provide clarity and, in conjunction to photography, the digital Leica DFC cameras images, it lets you control the microscope, illu-
with the motorized IsoPro™ cross-stage, even are exactly right for you. Together with the minator, stages, cameras and more. For addi-
images of large specimens can be captured Leica Application Suite, they provide virtually tional information, refer to the LAS online help.
step by step and automatically joined to create limitless freedom of use. For additional informa-
a new complete image. tion about Leica cameras, refer to the camera's
documentation.
Adapter
If camera control using the Leica Application
Suite is not required, conventional mirror reflex
and rangefinder cameras from third-party
manufacturers can be used. For this purpose,
Leica Microsystems offers a variety of adapters
that can be used together with the 50% and
100% trinocular tubes.
Application If there is unwanted shading at the corners even Cameras from third-party suppliers
All Leica DFC cameras are equipped with a with a compatible C-mount adapter installed, it In addition to Leica DFC cameras with the stan-
standardized C-mount interface. In turn, the can be corrected using the "Shading function" dardized C-mount interfaces, you can connect
C-mount adapter for the respective trinocular of the camera software. third-party cameras to the microscope using
tube is connected to this interface. This adapter a T2 bayonet adapter. To do so, instead of the
creates a solid mechanical connection between Alternatively, you can also use a C-mount C-mount adapter, simply use the correspond-
the microscope and camera and ensures opti- adapter with higher magnification. This primar- ing SLR adapter with T2 connection. However,
mum rendering of the microscopic image on ily avoids the critical border area of the field of these third-party cameras are not integrated
the image sensor of the camera. vision and concentrates on the center of the into the Leica Application Suite and have to
field of vision. be operated using the corresponding software
Usually, the ideal is for the digital camera to from the camera manufacturer.
capture as much of the field of vision as possi- Camera optimal suitable
ble, while excluding as much of the black edge (large (smaller The Leica digital cameras are detailed in a sepa-
of the field of vision as possible. To do so, the image field) image field) rate user manual along with instructions for
magnification factor of the C-mount adapter their assembly and use.
DFC295
must match the image format of the sensor as 0.5× 0.63×
DFC290 HD
closely as possible (see table).
DFC425
0.5× 0.63×
DFC425 C
DFC495 0.63× 0.8×
DFC500 0.63× 0.8×
To meet the various requirements regarding Achromatic objectives Achromatic objectives with a long focal
imaging properties, there is a choice of high- The 0.32×, 0.5×, 0.63×, 0.8×, 1×, 1.5×, 2× achro- length:
quality interchangeable planachromatic and matic objectives offer countless variants for For special applications achromatic objectives
planapochromatic objectives and also lower- selecting the object field diameter, magnification with long working distances and focal lengths
priced interchangeable achromatic objectives. ranges and working distances (see page 63). of f=100 mm to 400 mm are available.
adjusted in 10 increments.
The intensity of the illuminator can be Use 2. Adjust the brightness by briefly pressing
•
The light of the Leica LED3000 NVI™ can the or buttons.
be very bright. Therefore, always switch
on the illuminator before you look through the
The Leica LED3000 NVI™ can also be
controlled via the Leica Application Suite eyepieces!
(LAS) or the Leica SmartTouch.
1. Switch on the illuminator by briefly press-
ing the (ON/OFF) button.
between 50 mm and 150 mm.
The supported working distance is
adjusted in 10 increments.
The intensity of the illuminator can be Use 2. Adjust the brightness by briefly pressing
•
The light of the Leica LED5000 CXI can the or buttons.
be very bright. Therefore, always switch
on the illuminator before you look through the 3. Switch off the illuminator by briefly press-
The Leica LED3000 NVI™ can also be
controlled via the Leica Application Suite eyepieces! ing the button.
(LAS) or the Leica SmartTouch.
1. Switch on the illuminator by briefly press-
ing the (ON/OFF) button.
increased magnification level of 1.5×.
Using the Leica LED5000 CXI results in an
Leica M50
87
66
Leica M50 with small incident-light base, transmitted-light base, Leica L2 illumination
205 and 45° binocular tube 277
220 390
54
315
300
321
436
390
277
134
105 90
87
66
205 277 54
436
500
Leica M50
278
Leica M50 with large incident-light base and ErgoTube® 45°
116 119
301
24
514 255
436
322
500
301
116 119
54
278 301 278
24
255
Leica M-series User Manual 54
Leica M50
Leica M50 with small swinging-arm stand and 45° binocular tube 220
54
260
500
345
173
170
709
345 260
260
54
220
220
173
709
Leica M-series User Manual 55
330
Leica M60
324
24
Leica M60 with large incident light base and 45° binocular tube 116 119
278
301
54
66 255
330
288
324
301
24
116 119
278 54
301 278
255
66
24
116 119
Leica M80 with large incident-light base and 45° binocular tube
301
278
54
255
66
288
395
301
324
54
24
116 119
278
301
278
255
66
420
Leica M80
290
Leica M80 with transmitted-light base TL ST and binocular ErgoTube®
356 154
21
154
106
85
500
606
420
430
290
154
21
154
340
106
356 85
Leica M80 with standard swinging-arm stand and 45° binocular tube
220
54
598
470
345
173
345 230
794
330
330
54
220
220
173
794
Leica M-series User Manual 59
3
Leica M80 109 100
42
Leica M80 with Leica LED2500 and binocular ErgoTube® 331
257
max. 426
231
min. 290
331
300
109 100
42
54
257 331 257
max. 426
231
in. 290
max. 420
Leica M80
min. 290
83.4
369
400
28.5
600
292
600
631
max. 420
min. 290
83.4
369
54
28.5
400 600
Parameter Example
MO Magnification of objective MO 1× objective
ME Magnification of eyepiece ME 25×/9.5 eyepiece
z Magnification changer position z Zoom position 4
q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° q Coaxial reflected light 1.5×, tube factor
ErgoTube™ r Factor 1.25×
r Factor 1.25x if the planachromatic and planapochromatic
objectives of the MZ125/MZ16 are used on the MS5, MZ6,
MZ75 or MZ95
NFOV Field number of the eyepiece. Field numbers are printed on the
eyepieces: 10× = 21, 16× = 14, 25× = 9.5, 40× = 6.
Calculation example: magnification in binocular tube Calculation example: field of view diameter in the specimen
★★ Keep accessories in a dust-free place when ★★ Remove dust with a pneumatic rubber bulb
not in use. or with a soft brush.