Nutrients: Morinda Citrifolia Linn. (Noni) and Its Potential in Obesity-Related Metabolic Dysfunction
Nutrients: Morinda Citrifolia Linn. (Noni) and Its Potential in Obesity-Related Metabolic Dysfunction
Review
Morinda citrifolia Linn. (Noni) and Its Potential in
Obesity-Related Metabolic Dysfunction
Aline Carla Inada 1 , Priscila Silva Figueiredo 1 , Rosângela Aparecida dos Santos-Eichler 2 ,
Karine de Cássia Freitas 1 , Priscila Aiko Hiane 1 , Alinne Pereira de Castro 3 and
Rita de Cássia Avellaneda Guimarães 1, *
1 Post Graduate Program in Health and Development in the Central-West Region of Brazil, Federal University
of Mato Grosso do Sul-UFMS, Campo Grande 79079-900, MS, Brazil; [email protected] (A.C.I.);
[email protected] (P.S.F.); [email protected] (K.d.C.F.); [email protected] (P.A.H.)
2 Department of Pharmacology, Biomedical Science Institute, University of São Paulo, São Paulo 05508900, SP,
Brazil; [email protected]
3 Post-Graduate Program in Biotechnology, Catholic University Dom Bosco, Campo Grande 79117-900, MS,
Brazil; [email protected]
* Correspondence: [email protected]; Tel.: +55-67-3345-7445
Abstract: Cultural and economic shifts in the early 19th century led to the rapid development
of companies that made good profits from technologically-produced commodities. In this way,
some habits changed in society, such as the overconsumption of processed and micronutrient-poor
foods and devices that gave rise to a sedentary lifestyle. These factors influenced host-microbiome
interactions which, in turn, mediated the etiopathogenesis of “new-era” disorders and diseases,
which are closely related, such as obesity, type 2 diabetes mellitus, non-alcoholic fatty liver disease,
hypertension, and inflammatory bowel disease, which are characterized by chronic dysregulation
of metabolic and immune processes. These pathological conditions require novel and effective
therapeutic approaches. Morinda citrifolia (noni) is well known as a traditional healing plant due
to its medicinal properties. Thus, many studies have been conducted to understand its bioactive
compounds and their mechanisms of action. However, in obesity and obesity-related metabolic
(dysfunction) syndrome, other studies are necessary to better elucidate noni’s mechanisms of action,
mainly due to the complexity of the pathophysiology of obesity and its metabolic dysfunction. In this
review, we summarize not only the clinical effects, but also important cell signaling pathways in
in vivo and in vitro assays of potent bioactive compounds present in the noni plant which have been
reported in studies of obesity and obesity-associated metabolic dysfunction.
1. Introduction
The prevalence of obese individuals has doubled worldwide since the 1980s. In 2014, it was
estimated that more than 1.9 billion adults were overweight, corresponding to 39% of all adults in the
world. Of this latter group, 13% were already considered obese, i.e., 600 million [1]. In the United
States (USA), obesity is a problem that increased by approximately 50% among adults throughout the
1980s and 1990s [2] and may result in a reduction in longevity of the North American population [3].
Obesity is prevalent in low-income groups who often live in urban areas in the USA and Europe.
According to the World Health Organization (WHO), in 2015, more than 50% of adults were overweight
or obese in 46 countries across Europe, especially in the eastern part of the region. Nowadays,
overweight and obesity are estimated to result in the deaths of about 320,000 men and women in
20 countries in Western Europe every year [4]. In 2012, China’s Minister of Health indicated that in the
country with 1.2 billion individuals, 300 million Chinese were obese [5]. In Brazil, more than a half of
the Brazilian population was overweight (52.5%), of which 13.9% were considered obese in 2014 [6].
Although obesity is the result of an imbalance between energy intake and expenditure, it is likely
that a disturbed metabolism due to inadequate nutrition contributes to an abnormal or excessive
fat accumulation associated with impaired health. Central obesity is a sign of the most prevalent
chronic metabolic disorder of our era with important global public health challenges. Genetic factors,
together with inappropriate food supply, entertainment, and labor-saving devices, and the advertising
of highly-appealing foodstuffs by the food industry, results in energy-dense diets and decreased
physical activity. This constitutes a gene-environment interaction, where endocrine factors mediate
and stimulate some of the pathways that lead to obesity [1,7,8]. Recently, many groups have been
focusing their studies on the involvement of the gut microbiota in obesity and metabolic dysfunction.
In 2006, one of the first studies that found a relationship between gut microbiota and weight gain
reported that the latter was putatively caused by an increase in energy-harvesting capabilities of the
microbiota in obese persons [9].
Central, as opposed to peripheral, obesity predisposes individuals to metabolic abnormalities,
cardiometabolic complications, such as insulin resistance, type 2 diabetes mellitus (T2DM)
dyslipidemia, hypertension, and non-alcoholic fatty liver disease (NAFLD), which are components of
obesity-related metabolic syndrome (MetS) that put individuals at risk of developing cardiovascular
disease (CVD) [10,11]. Nowadays, the term ‘metabolic syndrome’ is used widely and is defined as
when an individual shows at least three of the following cardiovascular risk factors: central obesity
(excessive upper body and visceral fat), dyslipidemia (high triglycerides or low high density lipoprotein
cholesterol (HDL) levels), or hypertension or hyperglycemia (T2DM) [12].
There are various pharmacological and non-pharmacological options which are broadly accepted
as treatment and prevention of obesity and obesity-related diseases, including dietary control, physical
activity, lifestyle changes, weight-loss medications, weight-loss surgeries, or specific medications [13].
Various dietary patterns have been extensively studied for health promotion and to diminish the risks
of chronic diseases. A “healthy eating concept” has been put forward in which functional foods and
nutraceuticals are important parts [14].
In this context, according to Health Canada, the term nutraceutical is conceptualized as a product
isolated or purified from foods that is generally sold in medicine formulations not usually associated
with food. A nutraceutical has been shown to have a physiological benefit or provide protection
against chronic diseases. The term ‘functional food’ is distinguished as a food that is similar in
appearance to, or may be, a conventional food, that is consumed as part of a usual diet, and has
demonstrated physiological benefits and/or to reduce the risk of chronic disease beyond basic
nutritional functions [15].
Due to the complexity of obesity pathogenesis, the majority of the treatments are not related to
obesity alone, seeing that it is associated with other metabolic disorders, such as oxidative stress and/or
inflammatory alterations that can occur concomitantly in several tissues [11]. Recent studies have
focused on the development of innovative therapeutic agents from natural sources as an alternative
medicine. Nevertheless, the challenge of natural product studies is to evaluate, in a consistent way,
the mechanisms of action and bioactive compounds which could assure the beneficial effects of the
products [13,16].
Morinda citrifolia L. (noni) is an example of a plant used as a functional food and has been widely
studied due to its apparent beneficial effects on human health. It has been investigated as an alternative
in anticancer, antibacterial, and antimicrobial therapies, and in the treatment of esophageal reflux
and ulcers in animals [17–21]. In humans, there are few studies showing the beneficial effects of
noni. Sattar et al. [22] and Siddiqui et al. [23] demonstrated the effective benefits of a topical ointment
prepared from noni stem extract against cutaneous leishmaniasis. Palu et al. [24] showed a 25%
reduction in lipid peroxidation in the blood of athletes, after an endurance test, taking noni juice (NJ)
compared to controls, thereby demonstrating an antioxidant effect. The antioxidant properties of noni
Nutrients 2017, 9, 540 3 of 29
juice were also demonstrated by Wang et al. [25] in a study involving 132 heavy cigarette smokers.
They reported reduced plasma levels of superoxide anion radicals and lipid hydroperoxide, which
are considered biomarkers of degenerative diseases associated with cigarette smoking, in smokers
who drank NJ compared to smokers who did not. Moreover, Issell et al. [26] reported less fatigue and
maintenance of physical functions in patients with cancer.
Recently, more attention has been directed to the anti-obesity properties of the noni plant in
animal models. However, studies are still scarce in humans and more investigations are needed.
Therefore, the aim of this review was to assess specific in vitro and in vivo studies related to the
mechanisms of action and bioactive compounds that promote the benefits of Morinda citrifolia L. (noni)
in the treatment of obesity and obesity-related metabolic dysfunction, such as insulin resistance/T2DM,
dyslipidemia, hypertension, NAFLD [27,28], and its influence on the gut microbiota.
2.3. Important Phytochemicals of Morinda citrifolia on Obesity and Obesity-Related Metabolic Dysfunction
The high prevalence of obesity and obese-related metabolic dysfunction has led to extensive
investigations to seek out alternative therapies because of several reports of side-effects that are
promoted by synthetic drugs. Noni can provide important natural products that have been widely
studied and may be considered an alternative therapy for many diseases [14]. Many scientific
publications have shown that the noni plant contains a variety of nutritional and functional compounds.
However, our current knowledge of these compounds is still not satisfactory. Some studies have
demonstrated that the principal bioactive compounds from Morinda citrifolia have potentially beneficial
effects in obesity and obesity-related metabolic dysfunction, and they are listed in Table 1.
Table 1. Principal phytochemicals from Morinda citrifolia as bioactive compounds against obesity and
obesity-related metabolic dysfunction.
Concentrations of
Part of Plant Structural Class Bioactive Compounds References
Bioactive Compounds
Chlorogenic acid 10.49 mg/100 mL [43] [43–48]
Fruit Phenolic acid Gentisic acid 19.16 mg/100 mL [43] [43–45,48]
P-hydroxybenzoic acid 14.12 mg/100 mL [43] [43–45,48]
Anthocyanin
Data not shown [49]
(cyanidin-3-O-rutinoside)
Catechin 53.68 mg/g [50] [50,51]
Flavonoids Epicatechin 6.8 mg/g [51] [51]
Kaempferol 6.4 mg/g [51] [52,53]
Rutin 8.06 mg/g [50] [45,54–56]
Quercetin 7.4 mg/g [51] [49,54]
Iridoids Asperulosidic acid Data not shown [48,57]
Americanin A 17.4 mg [58] [58,59]
Americanol A 21 mg 60] [60]
Isoprincepin 14 mg [60] [60]
Lignans Lirioresinol B Data not shown [61]
Lirioresinol B dimethyl ether Data not shown [61]
Morindolin 10 mg [60] [60]
3,3’-Bisdemethypinoresinol 69 mg [60] [60]
Coumarins Scopoletin 46.1 mg [58] [49,55,58,62]
Minerals Potassium 3900 mg/L [38] [38,63–65]
Triterpenoids/ Ursolic acid Data not shown [61,66–68]
terpenes Saponin Data not shown [69]
Vitamin C Data not shown [70,71]
Vitamins
Vitamin E Data not shown [70,71]
Nutrients 2017, 9, 540 5 of 29
Table 1. Cont.
Concentrations of
Part of Plant Structural Class Bioactive Compounds References
Bioactive Compounds
Catechin 63.46 mg/g [50] [50]
Epicatechin 23.08 mg/g [50] [50]
Leaf Flavonoids
Rutin 6.83 mg/g [50] [45,50,55]
Kaempferol 21–80 mg [52] [52,66]
Triterpenoids/terpenes Ursolic acid Data not shown [61,68]
Deacetylasperulosidic acid Data not shown [73]
1,2-Dimethoxyanthraquinone 3.5 mg [72] [72]
Alizarin-2-methyl ether 11.3 mg [72] [72]
Rubiadin-1-methyl ether 15.5 mg [72] [72]
Root Anthraquinones
Lucidin 3-O-beta-D-primeveroside Data not shown [73]
Damnacanthol-3-O-beta-D-primeveroside Data not shown [73]
Morindone-6-O-beta-D-primeveroside Data not shown [73]
Asperulosidic acid Data not shown [73]
Iridoid
Principal bioactive compounds from fruits, leaves, and roots of Morinda citrifolia used for obesity and obesity-related
metabolic dysfunction.
These bioactive compounds, called phytochemicals, include phenolic acids, lignans, flavonoids,
flavones, flavans-3-ol, anthocyanins, phytosterols, alkaloids, vitamins, and minerals. In recent
decades, polyphenols have been the most important compounds shown to possess beneficial effects
against obesity and metabolic dysfunction. Polyphenols include phenolic acids, flavonoids, and
stilbenes, which are the most common compounds used in the development of natural products for
metabolism-associated disorders/diseases [13,44,45].
Phenolic acids are divided into two classes: hydroxycinnamic and hydroxybenzoic acids.
Hydroxycinnamic acids include o-coumaric acid, m-coumaric acid, caffeic acid, ferulic acid, and sinapic
acid, and are present in the form of simple esters with glucose or quinic acid. Hydroxybenzoic
acids include salicylic acid, gentisic acid, p-hydroxybenzoic acid, gallic acid, vanillic acid, and
3,4-dimethoxybenzoic acid. The most common acid derivative is chlorogenic acid [13,44,45].
Flavonoids are abundant compounds in nature and they are divided into six subgroups: flavonols,
flavanones, isoflavanoids, flavones, flavand-3-ol, and anthocyanins. Flavonoids have been widely
studied due to their therapeutic properties in the treatment of metabolic disorders due to their ability
to modulate the numbers of cell signaling pathways that affect carbohydrate digestion, fat deposition,
and the release rate of insulin or glucose uptake in insulin-responsive tissues [44,45,50,51].
Quinones are a class of organic compounds, where 9,10-anthraquinones (9,10 dioxoanthracenes)
are an important subgroup [74]. Anthraquinones in Morinda citrifolia are found especially in
the roots, and the main compounds with important effects on metabolism are alizarin, lucidin
3-O-β-D-primeveroside, damnacanthol-3-O-β-D-primeveroside, and rubiadin-1-methyl ether [72,73].
Coumarins are found in many edible plants and fruits. One of the most important coumarins
found in noni plant is scopoletin (6-methoxy-7-hydroxycoumarin), which has shown a notable
effect in the treatment of obesity and metabolic dysfunction [49,55]. Lignans and neolignans are
present in many plants, being a large group of natural products derived from the oxidative coupling
of two C6-C3 units [75]. The most important lignans isolated from noni fruit are americanin A,
americanol A, episesamin 2,6-dicatechol, isoprincepin, lirioresinol, lirioresinol B dimethyl ether,
morindolin, and 3,3’-bisdemethypinoresinol. These lignans improve parameters in obesity and
associated disorders/diseases [58,59,61,76].
Triterpenoids are the largest class of secondary metabolites produced by plants. Ursolic acid and
related triterpene compounds, such as oleanolic acid, betulinic acid, uvaol, and α- and β-amyrin are
widespread in many plants [77,78]. The most abundant triterpenoid from noni is ursolic acid, which
has been widely investigated because of its hypoglycemic property [61,67,68].
Iridoid is a monoterpene, differing from triterpenes, and it is derived from geraniol. Iridoid
glucosides and glycosides, a subclass of iridoid, are terpenes bound to glucose, or any sugar,
Nutrients 2017, 9, 540 6 of 29
respectively [79]. Asperulosidic acid is one of the most important iridoids isolated from
Morinda citrifolia, and it has been shown to improve blood fluidity, which influences the health
of obese patients and those with obesity-associated disorders, such as hypertension, diabetes
and dyslipidemia [57,80,81]. Vitamins (C, ascorbic acid, and E, α-tocopherol) are two important
non-enzymatic antioxidants that have important effects because of their free-radical scavenging
property [70,71].
3. Effects of Bioactive Compounds from the Morinda citrifolia L. Plant on Obesity and
Obesity-Related Metabolic Dysfunction
The influence of Morinda citrifolia fruit (MFE) and leaf (MLE) extracts on LPL activity were
evaluated in vitro by two independent research groups [50,51]. Pak-Dek et al. [50] studied MFE
and MLE with green tea (GTE) and catechin extracts on the enzymatic activity of LPL. The data
demonstrated that all extracts tested inhibited LPL activity substantially after 30 min of incubation.
However, the greatest inhibition of LPL activity was seen with 0.2 mg/mL MLE in a dose-dependent
manner when compared to MFE, GTE and catechin.
Sahib et al. [51] evaluated MFE, Momordica charantia (MCE) and Centella asiatica (CAE) extracts in
LPL inhibition and the effects of the extracts in proliferation and differentiation of 3T3-L1 preadipocytes
Nutrients 2017, 9, 540 10 of 29
(Table 2). The results showed that 1 mg/mL MFE exerted the most significant inhibitory effect on LPL,
and in a dose-dependent manner. On the other hand, after 24, 48, and 72 hours of extract incubation,
only MCE inhibited adipogenesis in the concentration range of 0–5 mg/mL and differentiation at the
highest concentration of 0.5 mg/mL at 48 h. Interestingly, the data revealed that all of the extracts
contained high concentrations of phenolic compounds, including catechin and epicatechin, which may
be the responsible agents for these effects [51].
Several studies attributed these effects on lipid metabolism to the phenolic compounds, especially
catechins present in the extracts [113,114]. However, due to the fact that catechins in MFE and MLE
were lower than in GTE [50], one explanation about the inhibition of LPL may be the synergistic effect of
catechin with other components present in the extracts since synergism between flavonoids is believed
to be better than with one alone. In fact, low-processed whole plant extracts supply multiple chemicals,
as much as food does, and depends on synergistic metabolic effects to confer health. In conclusion, the
groups suggested that MLE and MFE may be used as anti-obesity agents [50,115].
Polyphenols have been intensively used in studies of obesity and weight management, as well as
in other metabolic conditions [13,44,45]. The most used polyphenols include phenolic acids (gentisic
acid, p-hydroxybenzoic acid, and the derivative chlorogenic acid) and flavonoids (epicatechin, catechin,
rutin, quercetin, and kaempferol). Several transcriptional factors, such as proliferator-activated
receptor (PPAR)-γ and CCAAT/enhancer-binding proteins (C/EBPs), are involved in the early stage
of adipocyte differentiation [116]. PPAR-γ, for instance, influences glucose homeostasis and insulin
sensitivity [117].
Flavonoids and phenol acids were able to inhibit adipogenesis in 3T3-L1 adipocytes [45]. The
polyphenols rutin (flavonoid) and o-coumaric acid (phenol acid) showed the best results in the
inhibition of differentiation with lower levels. Moreover, these compounds were able to inhibit the
expression of PPAR-γ and C/EBPα protein levels, demonstrating that these polyphenols inhibit
adipogenesis by affecting the transcriptional factor cascade upstream of PPAR-γ expression and also
inhibiting the expression of leptin and upregulating adiponectin protein levels [45].
Chlorogenic acid has been claimed to modulate lipid and glucose metabolism in vivo in healthy,
as well as in metabolic disorder conditions [46,47]. Eight weeks of treatment with chlorogenic acid
exhibited important alterations in a model of HFD-obese male golden hamsters, decreasing body
weight gain and visceral adiposity, and ameliorating several metabolic parameters. Furthermore,
chlorogenic acid modified lipid and glucose metabolism due to (PPAR)-α action which, in turn,
regulated binding, transport, oxidation, and synthesis of free fatty acids (FFAs) [46]. Thus,
after activation of PPAR-α, the activity of FFA oxidation enzymes may increase elevating fat
energy utilization in the liver and muscle, ameliorating insulin tolerance, and decreasing insulin
resistance [118,119].
Additionally, another flavonoid that was isolated from the fruit and leaves of Morinda citrifolia
is kaempferol [52,66,120]. This flavonoid is the major component of soy leaves (SLE), and a recent
study evaluated the anti-obesity effects of SLE extracts in HFD-obese male C57BL/6 mice. Ten weeks
of treatment suppressed body weight gain and fat accumulation of WAT. Furthermore, kaempferol
supplementation (50 mg/kg/day) induced (i) a decrease in pro-inflammatory cytokine (TNFα and
IL-6) gene expression; (ii) a downregulation of adipogenesis-related genes, such as C/EBP-α, sterol
regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FAS); and (iii) an upregulation
of fat oxidation-related genes, such as hormone-sensitive lipase (HSL), carnitine palmitoyl transferase 1
(CPT-1), and uncoupling protein-2 (UCP-2), in WAT from HFD-obese mice. Similar results were
observed in 3T3-L1 adipocytes, as well [121].
and nutritional challenges [122]. T2DM is a difficult problem that has been increasing rapidly, and
insulin resistance has an important role in the pathogenesis of T2DM. The reconstitution of insulin
sensitivity is an important strategy for the treatment of T2DM. Thus, Morinda citrifolia has been widely
studied as an alternative treatment for these complications.
Intensive research efforts have evaluated the positive effects of Morinda citrifolia on glucose
homeostasis in models of T2DM [49,61,73,76]. Nguyen et al. [61] observed that methanolic
Morinda citrifolia extract (part not identified) showed an anti-diabetic effect in vitro. The extract
exhibited stimulatory effects on glucose uptake using a fluorescent-tagged glucose probe (2-NBDG) in
3T3-L1 adipocyte cells. The group identified two new lignans, three new neolignans, and 10 known
compounds, where the lignans and ursolic acid were the bioactive compounds that confirmed the
inhibitory effects on protein tyrosine phosphatase 1B-gene (PTP1B) and stimulatory effects on 2-NBDG.
In this study, lignans, such as episesamin 2,6-dicatechol, lirioresinol B, lirioresinol B dimethyl ether,
and ursolic acid, were considered the anti-diabetic effectors for the inhibition of PTP1B (Table 3).
Protein tyrosine phosphatases (PTPs) are a group of proteins that participate in intracellular signaling
and metabolism by dephosphorylating tyrosine residues. There are several PTPs, where PTP1b
has important roles in insulin receptor signaling [123] and is a key regulator of the leptin signaling
pathway [124].
Table 3. The effects of administration of the Morinda citrifolia L. plant on insulin resistance/type 2
diabetes mellitus (T2DM).
In other studies, lignans from Myristica fragrans Houtt. (nutmeg) demonstrated strong stimulation
AMPK activity in differentiated C2C12 cells. AMPK has been considered as a potential therapeutic
target for the treatment of metabolic syndrome, including obesity and T2DM [125]. Ursolic acid is one
of the most important triterpenoids isolated from various natural products, including Morinda citrifolia.
Nutrients 2017, 9, 540 12 of 29
Jayaprakasam et al. [67] isolated ursolic acid, as well as anthocyanins from Cornus mas (cornelian
cherry), and added them to the HFD for an additional eight weeks. The compounds diminished
obesity and glucose intolerance in HFD-obese C57Bl/6 mice to some extent [67]. Indeed, the beneficial
effects of acute (three days) and chronic (six weeks) treatment with ursolic acid was also reported by
others. These treatments increased skeletal muscle and brown fat metabolism which, in turn, increased
energy expenditure. These data were confirmed by the reduction of obesity, glucose intolerance, and
fatty liver in HFD-obese C57Bl/6 mice [68].
Another important study demonstrated that fermented noni juice (fNJ) administered to
HFD-fed C57Bl/6 male mice reduced body weight and improved glucose and insulin tolerance,
as well as fasting blood glucose. These authors detected scopoletin, quercetin, and anthocyanin
(cyanidin-3-O-rutinoside) in methanolic extracts of fNJ using HPLC. They suggested that the
anti-diabetic effects of fNJ may be associated with quercetin and anthocyanin [49] (Table 3).
Kampkotter et al. [54,126] demonstrated the properties of quercetin in resistance to oxidative stress in
an established model of Caenorhabditis elegans, which is an in vivo model that has become increasingly
popular to evaluate pharmacologically-active compounds of herbal origin. Quercetin not only had a
strong antioxidant capacity, but also prolonged the lifespan of Caenorhabditis elegans and was considered
a modulator of cell signaling processes to exert its protective properties.
Anthocyanins present in bilberry fruit extract ameliorated hyperglycemia and insulin sensitivity
in male KK-Aγ mice, a genetic model of T2DM. Anthocyanins activate AMPK, a signaling pathway
important because of its role in the control of hepatic glucose and lipid metabolism [127,128]. These
data corroborated with another study [67] that isolated anthocyanins (cyanidin 3-O-galactoside,
pelargonidin 3-O-galactoside, and delphinidin 3-O-galactoside) from Cornus mas (cornelian cherry).
HFD-obese mice that received anthocyanins exhibited a non-obese pattern in the glucose tolerance test
while HFD-obese mice showed substantial glucose intolerance [67]. Kaempferol and quercetin isolated
from Euonymus alatus were shown to improve insulin-stimulated glucose uptake in 3T3-L1 mature
adipocytes [53].
In addition, Zhang et al. [62] showed that scopoletin, a phenolic coumarin, had beneficial
effects on insulin-resistant HepG2 cells. Insulin resistance was evaluated by measuring PI3K-linked
protein kinase B/Akt (Akt/PKB). Thereafter, scopoletin was able to stimulate the reactivation of
insulin-mediated Akt/PKB phosphorylation, which was greater compared to the positive control
rosiglitazone, a thiazolidinedione and activator of PPARγ that markedly improves insulin and glucose
parameters in T2DM patients [129]. In 3T3-L1 adipocytes, scopoletin upregulated the expression of
PPARγ2, an isoform of PPARγ that has critical functions in adipocyte differentiation, lipid storage,
and glucose metabolism [130].
Accordingly, Lee et al. [76] also reported the beneficial effects of noni fruit in diabetes. They
used noni fruit powder fermented by Cheonggukjang, which is a fast-fermented soybean paste, and
bacteria, such as Bacillus subtilis (KCTC11352BP), Bacillus sonolensis (KCTC11354BP), Bacillus sp. (KCTC
11351BP) and Bacillus circulans (KCTC 11355BP). The data showed that a fNJ (FMC)-based diet, for
90 days was effective in reducing fasting glucose and glycosylated hemoglobin (HBA1c), enhancing
insulin sensitivity and decreasing LDL, triglycerides and cholesterol in KK-Aγ diabetic mice. These
responses are believed to be due to the activation of PPAR-γ and AMPK phosphorylation (Table 3).
In fact, when HEK293 cells were transfected with a plasmid containing the PPAR-γ
response-element-driven luciferase reporter gene, fermented noni extract activated the
PPAR-γ-dependent luciferase activity. In addition, this compound stimulated glucose uptake
in C2C12 culture cells via activation of the AMPK pathway. These effects could be due to the presence
of anthraquinones, flavonoids and terpenoids [76].
The evaluation of dried roots of Morinda citrifolia were extracted with methanol, suspended
in water (H2 O), and partitioned in different parts of chloroform (CHCl3 ), ethyl acetate (EtOAc),
and n-butanol (n-BuOH). Sequentially, the solvents were removed from these different parts in
order to generate the soluble phases: CHCl3 , EtOAc, n-BuOH, and H2 O. Therefore, the fractions
Nutrients 2017, 9, 540 13 of 29
of soluble phases of methanol extract from Morinda citrifolia roots (MRE) were administrated
orally to streptozotocin-induced ddY diabetic male mice (single administration). Only n-BuOH
exhibited a significant reduction of blood glucose levels after five hours of administration,
whereas methanol extract and other soluble phases did not display any hypoglycemic effects.
Hence, after isolation of compounds from the n-BuOH fraction, two iridoids and three
anthraquinones were identified, where two anthraquinones, lucidin (lucidin 3-O-β-D-primeveroside),
and damnacanthol-3-O-β-D-primeveroside, were responsible for the hypoglycemic effects [73]
(Table 3).
Likewise, anthraquinones are important agents in the treatment of diabetes [72]. Three
anthraquinones (1,2-dimethoxyanthraquinone, alizarin-2-methyl ether and rubiadin-1-methyl ether)
were isolated from the n-hexane and CHCl3 fractions of Morinda officinalis roots and used to investigate
fat accumulation in 3T3-L1 pre-adipocytes using the oil red O staining method. Alizarin-2-methyl
ether was the compound that produced the highest increase in adipocyte differentiation followed by
rubiadin-1-methyl ether and 1,2-dimethoxyanthraquinone [72].
Morinda citrifolia also displayed positive effects in streptozotocin (STPZ)-diabetic
rats [70,71,131,132]. In diabetic patients, wounds are very complex to manage due to impaired
wound-healing. Nayak et al. [131] evaluated the wound-healing effects of NJ on an excision wound
model in induced diabetic rats. These animals exhibited improvement in wound-healing after
consuming NJ. The wound area was reduced earlier and had less dead tissue at the wound site in
NJ-treated rats than in their respective controls. The authors correlated wound-healing improvement
with low fasting glucose, which was also found to be reduced. Triterpenoids and tannins are bioactive
compounds that promote wound-healing due to their astringent and antimicrobial properties,
promoting wound contraction and increasing the rate of epithelialization. Furthermore, these
substances, especially triterpenoids, may have hypoglycemic effects [133].
The anti-diabetic effects of fNJ could be seen in another STPZ-diabetic rat model [132]. A possible
explanation for these effects was the presence of saponins, triterpenes, steroids, flavonoids (rutin),
and cardiac glycosides in the extract. However, the group attributed these effects to triterpenes and
saponins, the principal compounds that show the highest specific actions on glucose metabolism.
Norberg et al. [69] reported that saponins may have a glucagon decreasing effect and may enhance
glucose utilization, thereby lowering blood glucose. Moreover, saponins stimulate insulin release
from the pancreas due to diminishing degradation of glucagon-like peptide (GLP). Triterpenoids have
already been indicated as beneficial agents in diabetes mellitus, especially in alloxan-induced mice,
improving symptoms of glycosuria and elevated blood sugar [134,135].
These data corroborate another study [70] in which aqueous and methanol MFE were administered
to STPZ-diabetic rats for one week before diabetes induction, three days during induction, and
five weeks afterwards. Both MFE reduced blood glucose, glycosylated hemoglobin, blood urea,
and creatinine levels, which were explained by the possible prevalence of antioxidants (vitamin C,
vitamin E, flavonoids, terpenoids, and anthraquinones) in these extracts. In the same animal model,
an antihyperglycemic effect and antioxidant activity were observed for ethanolic MFE given for
30 days [71].
These antioxidant properties were demonstrated by thiobarbituric acid reactive substance
(TBARS), hydroperoxidose, and enzymatic and non-enzymatic antioxidants, such as catalase (CAT),
glutathione, superoxide dismutase (SOD), and vitamins C and E, respectively. It is believed that these
beneficial effects of noni were due to the synergistic effect of several biologically-active ingredients in
the extract, which provides for the antioxidant nature of the extract [71]. Another synergistic effect of
components of NJ was observed in alloxan-diabetic Sprague-Dawley rats, whereas NJ given for four
weeks combined with insulin was more effective in lowering fasting glucose levels compared to the
use of NJ or insulin alone [136].
Nutrients 2017, 9, 540 14 of 29
Table 4. Effects of the administration of the Morinda citrifolia L. plant on non-alcoholic fatty liver disease
(NAFLD).
All of these beneficial effects are possibly due to the large amount of phenolic acids present in
NJ. Large amounts of phenolic acids, such as gentisic acid, p-hydroxybenzoic acid, and chlorogenic
acid, are the dominant compounds in this juice [43]. Many studies have demonstrated that phenolic
compounds act as reactive oxygen species (ROS) scavengers, reducing lipid peroxidation, as well.
In vitro studies conducted by Joshi et al. [142] pointed to gentisic acid as the antioxidative and ROS
scavenging agent. If those effects are also beneficial to humans it remains unknown and further study
is necessary.
The effects of noni juice compounds have been extensively studied in animals. Chlorogenic
acid was able to enhance the activity of the important antioxidant enzymes SOD, CAT and GSH-Px
in STPZ-nicotinamide-induced type 2 diabetic rats [143]. Complementary studies reported that
aqueous extract of Mesona procumbens, which has chlorogenic acid as a major compound, has
anti-inflammatory action via the upregulation of antioxidants and downregulation of pro-inflammatory
biomarkers (TNF-α, iNOS and COX-2) [144], and it exhibits anti-obesity effects and improves lipid
metabolism [145].
In corroboration of the hepatic benefits of fNJ supplementation in an obese model,
Nerurkar et al. [49] demonstrated that fNJ produced positive effects on plasma glucose
levels by modulating hepatic gene expression of phosphoenolpyruvate carboxykinase (PEPCK),
glucose-6-phosphatase (G6P) and glucokinase (GCK). PEPCK and G6P are important gluconeogenic
enzymes regulated by insulin. They were inhibited after fNJ supplementation, which was confirmed
with HepG2 culture cells treated with FOXO1 siRNA and fNJ. GCK was upregulated by fNJ via
forkhead box O1 (FOXO1) transcription factor phosphorylation. The hypoglycemic properties of fNJ
were associated with the inhibition of hepatic FOXO1 mRNA with concomitant increase in FOXO1
phosphorylation. Consequently, fNJ improved hepatic insulin resistance indicated by homeostatic
model assessment-insulin resistance (HOMA-IR) (Table 4).
Those effects may be attributed to flavonoids, quercetin, and anthocyanins, specifically
cyanidin-3-O-rutinoside, which were isolated from methanolic extracts of fNJ. Some studies
demonstrated the inhibitory effect of anthocyanins on oxidative stress via FOXO transcription factor
regulation in Caenorhabiditis elegans [54,126]. In support of these studies, fNJ promoted the reduction of
hepatocyte fatty degeneration (smaller fatty globules and less numerous) in a model of STPZ-diabetic
rats. It was suggested that the hepatoprotective activity of Morinda citrifolia was due to the antioxidant
activity of flavonoid constituents [132].
Anthocyanins (delphinidin and cyanidin) isolated from Hibiscus sabdafera extract (HSE) also
showed positive effects against obesity and liver damage in HFD-obese hamsters. HSE and
anthocyanins regulated total body weight and visceral fat, reduced serum cholesterol and triglyceride
levels, protected against oxidation-associated damage in liver by regulating a liver antioxidant enzyme
(paraoxonase 1), and also reduced liver damage biomarkers ALT and AST [146].
Another study evaluated the benefits of scopoletin in reducing obesity and liver damage by
supplementing the diet with two doses of scopoletin in a HFD model of obese mice. Supplementation
resulted in reduced body weight, visceral fat, pro-inflammatory adipokine serum levels (leptin, MCP-1,
TNF-α, IL-6, IFNγ), insulin resistance, and hepatic lipid accumulation and, on the other hand, increased
serum adiponectin and fecal lipid levels. Moreover, supplementation was able to downregulate genes,
such as CIDEA (cell death-inducing DFFA-like effector A) and Apoa4 (apolipoprotein A-IV), which
are known to be related to hepatic steatosis and inflammation [147].
atherosclerosis [149–151]. Moreover, these factors are serious pathological conditions for endothelium
damage, causing cell proliferation, vascular remodeling, apoptosis, and enhancement of cellular
permeability with adhesion molecules that bind monocytes and T lymphocytes. The latter cells are
redirected into the intima vasculature by pro-inflammatory and chemoattractant cytokines. Hence,
monocytes differentiate into macrophages which overloaded excessive oxidized LDL, become foam
cells, elaborate cytokines, and then form atherosclerotic plaques [148].
The atherogenic dyslipidemic phenotype is characterized by high plasma triglycerides, low
levels of high-density lipoprotein cholesterol (HDL), and excessive LDL. Additionally, postprandial
(non-fasting) triglycerides (postprandial hyperlipidemia) are also an important component of
atherosclerosis [152]. The modern synthetic drugs that have been used as treatment for lipid
abnormalities are effective at reversing the measured signs, such as decreased LDL levels, but are
difficult to afford for many patients and are associated with several side-effects [153]. Morinda citrifolia
has been demonstrated to be an alternative therapy for this problem. A current study evaluated the
effects of NJ on serum lipid profiles in 132 heavy smokers (drinking 29.5 mL to 188 mL of NJ per day).
Heavy smoker volunteers who drank NJ displayed a reduction in cholesterol levels, triglycerides, and
high-sensitivity C-reactive protein (hs-CRP), a decrease in LDL and homocysteine, and an increase in
HDL fraction [25].
However, the few human studies available did not address this issue. Thus, clinical trials are
necessary to validate the beneficial qualities of Morinda citrifolia bioactive compounds in human
metabolic diseases.
In animals, a recent study performed by Shoeb et al. [111] demonstrated that supplementation with
two doses of fNJ in cholesterol-rich HFD-induced hyperlipidemia rats showed a significant decrease
in total cholesterol, triglycerides, and LDL at both doses when compared to the hyperlipidemic group.
The decrease in total cholesterol was observed with the lower dose of fNJ reaching similar values
as the positive control atorvastatin (10 mg/kg). Furthermore, the lower dose reduced body weight
compared to the hyperlipidemic group and it was also comparable to the hyperlipidemic group
receiving atorvastatin (Table 5).
These data corroborate the hypolipidemic effects of noni in a work done by Mandukhail et al. [154].
These authors compared ethanolic extracts of different parts of Morinda citrifolia and evaluated different
doses of MFE, MLE, and MRE extracts in tyloxapol (Triton WR 1339)-induced hyperlipidemia and
HFD-induced dyslipidemia models, both in rats. The highest dose of all extracts produced a significant
reduction in total cholesterol and triglyceride levels in the WR 1339 rat group. In contrast, the
HFD-induced dyslipidemia group showed different results depending on the extracts at the highest
dose of each. Both MFE and MLE prevented the rise in total cholesterol, LDL, total cholesterol/HDL
ratio, and atherogenic index without significant effects on HDL. However, MLE prevented the increase
in glucose levels and body weight in these animals, while MRE was the best extract in this study,
which proved to prevent the rise in all lipid and glucose levels and, at the same time, increasing HDL
and preventing weight gain, suggesting antidyslipidemic mechanisms for various parts of the noni
plant (Table 5).
Hypolipidemic and other positive effects of Morinda citrifolia were suggested in two
studies [111,154] that demonstrated the presence of strong antioxidant activity in the noni plant.
According to previous studies, Kamiya et al. [60] demonstrated the effects of fruits of Morinda citrifolia
in preventing atherosclerosis. MFE and its soluble phases (CHCl3 , EtOAc, n-BuOH, H2 O) inhibited
copper-induced LDL oxidation according to the TBARS method. Lignans were isolated in
the EtOAc-soluble phase, including 3,30 -bisdemethypinoresinol, americanol A, morindolin, and
isoprincepin, which showed remarkable or strong antioxidant activity. Thus, lignan compounds
of noni fruit are involved in the prevention of atherosclerosis, likely due to their numerous phenolic
hydroxyl groups (Table 5).
Nutrients 2017, 9, 540 17 of 29
Furthermore, Lin et al. [48] evaluated supplementation with fNJ at different concentrations
in HFD-cholesterol hamsters. They found that the group supplemented with fNJ displayed
hypolipidemic and antioxidative effects, demonstrated by decreases in serum triglyceride, total
cholesterol, atherogenic index, malondialdehyde levels, and hepatic lipids, while antioxidant activity
(TEAC and GSH) and fecal lipids were increased (Table 5).
To evaluate the effect of fNJ on lipid metabolism and mechanisms of actions, important genes
related to lipid homeostasis were evaluated in the liver, which is the major organ in the regulation
of lipid homeostasis. The data showed that SREBP-1c was upregulated after fNJ treatment; this is an
important transcription factor that stimulates the expression of lipogenic genes, such as that of fatty
acid synthase (FAS). This enzyme is responsible for the biosynthesis of FA. In energy expenditure,
Nutrients 2017, 9, 540 18 of 29
Previous studies have demonstrated that rutin and scopoletin are important phenolic compounds
that affect the cardiovascular system, including blood pressure regulation. Rutin possesses
renal-protective activity probably by inhibiting ROS production and through antioxidant activities,
reducing elevated malondialdehyde levels and restoring depleted manganese-superoxide dismutase
(MnSOD) and GSH, with positive effects on biochemical parameters, as well as on the histopathological
morphology of the kidneys [56]. Scopoletin also demonstrated hypotensive effects and relaxation
Nutrients 2017, 9, 540 19 of 29
of rat aorta. In addition, a possible inhibitory activity of angiotensin converting enzyme-1 (ACE1)
was suggested as a property of this phenolic compound [155,156]. Asperulosidic acid, an iridoid
glycoside present in MFE, showed substantial positive effects on blood fluidity and improved certain
lifestyle-related diseases, such as hypertension, dyslipidemia, and diabetes [80].
MRE showed antispasmodic and vasodilator activities mediated through blockade of
voltage-dependent calcium channels in isolated tissues of rats, guinea pigs, and rabbits [93] (Table 6).
These effects were mediated by alkaloids, phenolic compounds, sterols, flavonoids, tannins, coumarins,
and anthraquinones, which corroborate a study on Zingiber officinale Roscoe (ginger) that evaluated its
hypotensive effects [157]. The same bioactive compounds that were found in the noni plant were also
detected in ginger and could affect isolated tissues of rats, rabbits, and guinea pigs. The crude extract
of ginger decreased blood pressure and exhibited a cardiodepressant activity, with the activity being
mediated by Ca+2 channel-blocking, which was demonstrated when crude ginger extract shifted the
Ca+2 dose-response curve to the right, mimicking the effect of the positive control verapamil.
Considering that diuretics are used in the treatment of hypertension, Shenoy et al. [63] evaluated
the diuretic potential of NJ in normal rats. The effects observed were an increase in urine volume
in a dose-dependent manner with augmentation of the diuretic index accompanied by a significant
decrease in sodium and potassium ion excretion. The authors demonstrated that the noni plant had
aquaretic, instead of diuretic, actions (Table 6). Some authors believe that herbs act only as aquaretic
agents, which increase water excretion without affecting renal handling of electrolytes. In other words,
aquaretics only increase urine output, acting on the glomerulus, unlike conventional diuretic drugs
that act further along the nephron [63,64].
Herbs often contain large amounts of minerals (electrolytes) and noni fruit has a high content
of potassium. Hook et al. [65] evaluated the diuretic effect of Taraxacum officinale Weber (dandelion)
in normal mice and did not observe any significant variation in electrolytes (Na+ , K+ , Ca+2 ), but the
final volume of urine produced after five hours was greater than with the positive control, furosemide.
That study concluded that the high potassium content of dandelion was responsible for any diuretic
activity, where dandelion was similar in action to noni fruit and, thus, the increased urinary volume
could be suggestive of an osmotic effect [63,65].
In addition, noni powder also has a prebiotic action. One of the reasons may be the high amount
of polysaccharides in the fruit, since carbohydrates, except for starch, act as dietary fiber, coming
intact with the gut and contacting the bacterial community present in the intestinal microbiota [164].
The high molecular weight fraction of NJ is mostly composed of pectic polysaccharides, including
rhamnogalacturonan, homogalacturonan, and the neutral side chains of (arabino) galactan and
arabinan [165].
Another reason may be the high phenolic composition of the fruit, which may also have a
prebiotic function [162], such as quercetin and proanthocyanidin [166,167]. Previous studies have also
indicated that phenolic compounds can inhibit the growth of pathogens such as Escherichia coli and
Helicobacter pylori [166].
In addition to the microbiota, the size and height of the villi are important for intestinal function.
Diet plays an important role in intestinal morphology [163]. The mucus layer in the small intestine
protects the epithelial cells of the small intestine and mediates nutrient transport between the lumen
and the membrane of the brush border. The ontogeny of the whole gut has extensive implications for
intestinal function [168].
Supplementation with 1% noni fruit powder caused an increase in villus height, villus surface
area, and crypt depth when compared with control [163]. Although few studies have evaluated noni
fruit with regard to the microbiota and intestinal function, the in vitro and in vivo results presented in
Table 7 show that noni fruit shows prebiotic activity, when administered alone, and probiotic activity,
when used in fNJ, improving bacterial colonization and intestinal morphology.
4. Conclusions
Bioactive compounds from natural resources are a promising field of study for alternative
medicines, where Morinda citrifolia Linn. (noni) is one of these important options. Noni has
demonstrated positive effects in metabolic dysfunction, including the regulation of body weight
and fat deposits, lipid and glucose metabolism and blood pressure, hepatoprotective effects, and
improvement in bacterial colonization of the gut and intestinal morphology. However, Morinda citrifolia
has never been an important food plant, probably due to its poor palatability and toxicity, in its
natural homeland. This review reports the influence of the noni plant and its bioactive compounds,
emphasizing the potential mechanisms of action and effects on cell signaling pathways involved in
obesity and obesity-related metabolic dysfunction. The noni plant may be processed for some possible
medicinal properties after the management of toxicity, or some parts of the plant may be mixed with
Nutrients 2017, 9, 540 21 of 29
less nutritional, but appetizing, food for health benefits. Therefore, doses and the time of treatment or
long-term supplementation, and also new alternatives in the near future to improve the bioavailability
of the product, are very important issues to be evaluated. Finally, since Morinda citrifolia contains
important bioactive compounds for health, it may be an alternative therapeutic resource with great
potential in the treatment of obesity and obesity-related metabolic dysfunction.
Acknowledgments: We thank the post-graduate program in health and development in the Central-West Region
of Brazil, Federal University of Mato Grosso do Sul-UFMS, and the post-graduate program in biotechnology,
Catholic University Dom Bosco for their support. A. Leyva provided English editing of the manuscript.
Author Contributions: Aline Carla Inada, Priscila Silva Figueiredo, Rosângela Aparecida dos Santos-Eichler,
Alline Pereira de Castro, and Rita de Cássia Avellaneda Guimarães: assistance with structuring of the review,
writing, and literature review; Priscila Aiko Hiane and Karine de Cássia Freitas: assistance with structuring of
the review.
Conflicts of Interest: The authors report no conflict of interest.
References
1. World Health Organization. Obesity and Overweight. Available online: http://www.who.int/mediacentre/
factsheets/fs311/en/ (accessed on 12 November 2016).
2. Flegal, K.M.; Carroll, M.D.; Ogden, C.L.; Johnson, C.L. Prevalence and trends in obesity among U.S. adults,
1999–2000. JAMA 2002, 303, 235–241. [CrossRef] [PubMed]
3. Olshanky, S.J.; Passaro, D.J.; Hershow, R.C.; Layden, J.; Carnes, B.A.; Brody, J.; Hayflick, L.; Butler, R.N.;
Allison, D.B.; Ludwig, D.S. A potential decline in life expectancy in the United States in the 21st century.
N. Engl. J. Med. 2005, 352, 1138–1145. [CrossRef] [PubMed]
4. World Health Organization Regional Office for Europe. Available online: http://www.euro.who.int/__
data/assets/pdf_file/0003/294474/European-Food-Nutrition-Action-Plan-20152020-en.pdf (accessed on
12 November 2016).
5. World Health Organization. Obesity and Diabetes: The Slow-Motion Disaster Keynote Address at the 47th
Meeting of the National Academy of Medicine. Available online: http://www.who.int/dg/speeches/2016/
obesity-diabetes-disaster/en/ (accessed on 12 November 2016).
6. ABESO—Associação Brasileira Para o Estudo da Obesidade e da Síndrome Metabólica. Mapa da
Obesidade. Available online: http://www.abeso.org.br/atitude-saudavel/mapaobesidad (accessed on
12 November 2016).
7. Patel, J.J.; Rosenthal, M.D.; Miller, K.R.; Codner, P.; Kiraly, L.; Martindale, R.G. The critical care obesity
paradox and implications for nutrition support. Curr. Gastroenterol. Rep. 2016, 18, 45. [CrossRef] [PubMed]
8. Reddon, H.; Gueant, J.L.; Meyre, D. The importance of gene-environment interactions in human obesity.
Clin. Sci. 2016, 130, 1571–1597. [CrossRef] [PubMed]
9. Turnbaugh, P.J.; Ley, R.E.; Mahowald, M.A.; Magrini, V.; Mardis, E.R.; Gordon, J.I. An obesity-associated gut
microbiome with increased capacity for energy harvest. Nature 2006, 444, 1027–1031. [CrossRef] [PubMed]
10. Van Gaal, L.F.; Mertens, I.L.; De Block, C.E. Mechanisms linking obesity with cardiovascular disease. Nature
2006, 444, 875–880. [CrossRef] [PubMed]
11. Gross, B.; Pawlak, M.; Lefebvre, P.; Staels, B. PPARs in obesity-induced T2DM, dyslipidaemia and NAFLD.
Nat. Rev. Endocrinol. 2017, 13, 36–49. [CrossRef] [PubMed]
12. Mohamed, S. Functional foods against metabolic syndrome (obesity, diabetes, hypertension and
dyslipidemia) and cardiovasular disease. Trends Food Sci. Technol. 2014, 35, 114–128. [CrossRef]
13. Sun, N.N.; Wu, T.Y.; Chau, C.F. Natural dietary and herbal products in anti-obesity treatment. Molecules
2016, 21, 77–91. [CrossRef] [PubMed]
14. Singh, D.R. Morinda citrifolia L. (Noni): A review of the scientifica validation for its nutritional and
therapeutical properties. J. Diabetes Endocrinol. 2012, 3, 77–91. [CrossRef]
15. Health Canada. Policy Paper—Nutraceuticals/Functional Foods and Health Claims on Foods. Available
online: http://www.hc-sc.gc.ca/fn-an/label-etiquet/claims-reclam/nutra-funct_foods-nutrafonct_aliment-
eng.php (accessed on 15 November 2016).
16. Arulselvan, P.; Fard, M.T.; Tan, W.S.; Gothai, S.; Fakurazi, S.; Norhaizan, M.E.; Kumar, S.S. Role of antioxidants
and natural products in inflammation. Oxid. Med. Cell. Longev. 2016, 2016. [CrossRef] [PubMed]
Nutrients 2017, 9, 540 22 of 29
17. Zhang, W.M.; Wang, W.; Zhang, J.J.; Wang, Z.R.; Wang, Y.; Hao, W.J.; Huang, W.Y. Antibacterial constituents
of Hainan Morinda citrifolia (noni) leaves. J. Food Sci. 2016, 81, M1192–M1196. [CrossRef] [PubMed]
18. Abou Assi, R.; Darwis, Y.; Abdulbaqi, I.M.; Khan, A.A.; Vuanghao, L.; Laghari, M.H. Morinda citrifolia (noni):
A comprehensive review on its industrial uses, pharmacological activities, and clinical trials. Arab. J. Chem.
2015. [CrossRef]
19. Mahattanadul, S.; Ridtitid, W.; Nima, S.; Phdoongsombut, N.; Ratanasuwon, P.; Kasiwong, S. Effects of
Morinda citrifolia aqueous fruit extract and its biomarker scopoletin on reflux esophagitis and gastric ulcer in
rats. J. Ethnopharmacol. 2011, 134, 243–250. [CrossRef] [PubMed]
20. Hiramatsu, T.; Imoto, M.; Koyano, T.; Umezawa, K. Induction of nornal phenotypes in ras-transformed cells
by damnacanthal from Morinda citrifolia. Cancer Lett. 1993, 73, 161–166. [CrossRef]
21. Anekpankul, T.; Goto, M.; Sasaki, M.; Pavasant, P.; Shotipruk, A. Extraction of anti-cancer damnacanthal
from roots of Morinda citrifolia by subcritical water. Sep. Purif. Technol. 2007, 55, 343–349. [CrossRef]
22. Sattar, F.A.; Ahmed, F.; Ahmed, N.; Sattar, S.A.; Malghani, M.A.; Choudhary, M.I. A double-blind,
randomized, clinical trial on the antileishmanial activity of a Morinda citrifolia (noni) stem extract and
its major constituents. Nat. Prod. Commun. 2012, 7, 195–196. [PubMed]
23. Siddiqui, B.S.; Sattar, F.A.; Begum, S.; Dar, A.; Nadeem, M.; Gilani, A.H.; Mandukhail, S.R.; Ahmad, A.;
Tauseef, S. A note on anti-leishmanial, spasmolytic and spasmogenic, antioxidant and antimicrobial activities
of fruits, leaves and stem of Morinda citrifolia Linn—An important medicinal and food supplement plant.
Med. Aromat. Plants 2014, 3, 1–3. [CrossRef]
24. Palu, A.K.; Seifulla, R.D.; West, B.J. Morinda citrifolia L. (noni) improves athlete endurance: Its mechanisms of
action. J. Med. Plant. Res. 2008, 2, 154–158.
25. Wang, M.Y.; Peng, L.; Weidenbacher-Hoper, V.; Deng, S.; Anderson, G.; West, B. Noni juice improves serum
lipid profiles and other risk markers in cigarette smokers. Sci. World J. 2012, 2012, 1–8. [CrossRef] [PubMed]
26. Issell, B.F.; Gotay, C.C.; Pagano, I.; Franke, A.A. Using quality of life measures in a phase I clinical trial of
noni in patients with advanced cancer to select a phase II dose. J. Diet. Suppl. 2009, 6, 1–11. [CrossRef]
[PubMed]
27. Tilg, H.; Moschen, A.R.; Roden, M. NAFLD and diabetes mellitus. Nat. Rev. Gastroenterol. Hepatol. 2016.
[CrossRef] [PubMed]
28. Wang, F.; Han, L.; Hu, D. Fasting insulin, insulin resistance and risk of hypertension in the general population:
A meta-analysis. Clin. Chim. Acta 2016, 464, 57–63. [CrossRef] [PubMed]
29. Chan-Blanco, Y.; Vaillant, F.; Mercedes Perez, A.; Reynes, M.; Brillouet, J.-M.; Brat, P. The noni fruit
(Morinda citrifolia L.): A review of agricultural research, nutritional and therapeutic properties. J. Food
Comp. Anal. 2006, 19, 645–654. [CrossRef]
30. Pawlus, A.D.; Kinghorn, A.D. Review of the ethnobotany, chemistry, biological activity and safety of the
botanical dietary supplement Morinda citrifolia (noni). J. Pharm. Pharmacol. 2007, 59, 1587–1609. [CrossRef]
[PubMed]
31. Wang, M.; Kikuzaki, H.; Jin, Y.; Nakatani, N.; Zhu, N.; Csiszar, K.; Boyd, C.; Rosen, R.T.; Ghai, G.; Ho, C.T.
Novel glycosides from noni (Morinda citrifolia). J. Nat. Prod. 2000, 63. [CrossRef]
32. Heinicke, R.M. The pharmacologically active ingredient of noni. Pac. Trop. Bot. Gard. Bull. 1985, 15, 10–14.
33. Dixon, A.R.; McMillan, H.; Etkin, N.L. Ferment this: The transformation of noni traditional Polynesian
medicine (Morinda citrifolia, Rubiaceae). Econ. Bot. 1999, 53, 51–68. [CrossRef]
34. Nerurkar, P.V.; Hwang, P.W.; Saksa, E. Anti-diabetic potential of noni: The yin and the yang. Molecules 2015,
20, 17684–17719. [CrossRef] [PubMed]
35. Samoylenko, V.; Zhao, J.; Dunbar, D.C.; Khan, I.A.; Rushing, J.W.; Muhammad, I. New constituents from
noni (Morinda citrifolia) fruit juice. J. Agric. Food Chem. 2006, 54, 6398–6402. [CrossRef] [PubMed]
36. Motshakeri, M.; Ghazali, H.M. Nutritional, phytochemical and commercial quality of noni fruit:
A multi-beneficial gift from nature. Trends Food Sci. Technol. 2015, 45, 118–129. [CrossRef]
37. Nelson, S.C. Noni cultivation in Hawaii. Fruit Nuts 2001, 4, 1–4.
38. Chunhieng, M.T. Development of New Food Health Tropical: Application at the Nuts bre’ sil Bertholettia
Excelsa and the Fruit of Morinda citrifolia Cambodia. Ph.D Thesis, I'Institut National Polytechnique de
Lorraine (INPL), Nancy, France, 2003.
39. Peerzada, N.; Renaud, S.; Ryan, P. Vitamin C and elemental composition of some bushfruits. J. Plant Nutr.
1990, 13, 787–793. [CrossRef]
Nutrients 2017, 9, 540 23 of 29
40. Aalbersberg, W.G.L.; Hussein, S.; Sotheeswaran, S.; Parkinson, S. Carotenoids in the leaves of Morinda citrifolia.
J. Herbs Spices Med. Plants 2014, 2, 51–54. [CrossRef]
41. Millonig, G.; Stadlmann, S.; Vogel, W. Herbal hepatoxicity: Acute hepatitis caused by a noni preparation
(Morinda citrifolia). Eur. J. Gastroenterol. Hepatol. 2005, 17, 445–447. [CrossRef] [PubMed]
42. Yang, J.; Gadi, R.; Thomson, T. Antioxidant capacity, total phenols, and ascorbic acid content of noni
(Morinda citrifolia) fruits and leaves at various stages of maturity. Micronesica 2011, 41, 167–176.
43. Lin, Y.L.; Chang, Y.Y.; Yang, D.J.; Tzang, B.S.; Chen, Y.C. Beneficial effects of noni (Morinda citrifolia L.) juice
on livers of high-fat dietary hamsters. Food Chem. 2013, 140, 31–38. [CrossRef] [PubMed]
44. Badimon, L.; Vilahur, G.; Padro, T. Nutraceuticals and atherosclerosis: Human trials. Cardiovasc. Ther. 2010,
28, 202–215. [CrossRef] [PubMed]
45. Hsu, C.L.; Yen, G.C. Effects of flavonoids and phenolic acids on the inhibition of adipogenesis in 3T3-L1
adipocytes. J. Agric. Food Chem. 2007, 55, 8404–8410. [CrossRef] [PubMed]
46. Li, S.Y.; Chang, C.Q.; Ma, F.Y.; Yu, C.L. Modulating effects of chlorogenic acid on lipids and glucose
metabolism and expression of hepatic peroxisome proliferator-activated receptor-α in golden hamsters fed
on high fat diet. Biomed. Environ. Sci. 2009, 22, 122–129. [CrossRef]
47. Ong, K.W.; Hsu, A.; Tan, B.K. Anti-diabetic and anti-lipidemic effects of chlorogenic acid are mediated by
AMPK activation. Biochem. Pharmacol. 2013, 85, 1341–1351. [CrossRef] [PubMed]
48. Lin, Y.L.; Chou, C.H.; Yang, D.J.; Chen, J.W.; Tzang, B.S.; Chen, Y.C. Hypolipidemic and antioxidative effects
of noni (Morinda citrifolia L.) juice on high- fat/cholesterol-dietary hamsters. Plant Foods Hum. Nutr. 2012, 67,
294–302. [CrossRef] [PubMed]
49. Nerurkar, P.V.; Nishioka, A.; Eck, P.O.; Johns, L.M.; Volper, E.; Nerurkar, V.R. Regulation of glucose
metabolism via hepatic forkhead transcription factor 1 (FOXO1) by Morinda citrifolia (noni) in high-fat
diet-induced obese mice. Br. J. Nutr. 2012, 108, 218–228. [CrossRef] [PubMed]
50. Pak-Dek, M.S.; Abdul-Hamid, A.; Osman, A.; Soh, C.S. Inhibitory effect of Morinda citrifolia L. on lipoprotein
lipase activity. J. Food Sci. 2008, 73, C595–C598. [CrossRef] [PubMed]
51. Sahib, N.G.; Hamid, A.A.; Kitts, D.; Purnama, M.; Saari, N.; Abas, F. The effects of Morinda citrifolia,
Momordica charantia and Centella asiatica extracts on lipoprotein lipase and 3T3-L1 preadipocytes. J. Food
Biochem. 2011, 35, 1186–1205. [CrossRef]
52. Sang, S.; Cheng, X.; Zhu, N.; Stark, R.E.; Badmaev, V.; Ghai, G.; Rosen, R.T.; Ho, C.-T. Flavonol glycosides
and novel iridoid glycoside from the leaves of Morinda citrifolia. J. Agric. Food Chem. 2001, 49, 4478–4481.
[CrossRef] [PubMed]
53. Fang, X.K.; Gao, J.; Zhu, D.N. Kaempferol and quercetin isolated from Euonymus alatus improve glucose
uptake of 3T3-L1 cells without adipogenesis activity. Life Sci. 2008, 82, 615–622. [CrossRef] [PubMed]
54. Kampkotter, A.; Nkwonkam, C.G.; Zurawski, R.F.; Timpel, C.; Chovolou, Y.; Watjen, W.; Kahl, R.
Investigations of protective effects of the flavonoids quercetin and rutin on stress resistance in the model
organism Caenorhabditis elegans. Toxicology 2007, 234, 113–123. [CrossRef] [PubMed]
55. Wigati, D.; Anwar, K.; Sudarsono; Nugroho, A.E. Hypotensive activity of ethanolic extracts of
Morinda citrifolia L. leaves and fruit in dexamethasone-induced hypertensive rat. J. Evid. Based Complement.
Altern. Med. 2017, 22, 107–113. [CrossRef] [PubMed]
56. Korkmaz, A.; Kolankaya, D. Protective effect of rutin on the ischemia/reperfusion induced damage in rat
kidney. J. Surg. Res. 2010, 164, 309–315. [CrossRef] [PubMed]
57. Kamiya, K.; Tanaka, Y.; Endang, H.; Umar, M.; Satake, T. New anthraquinone and iridoid from the fruits of
Morinda citrifolia. Chem. Pharm. Bull. 2005, 53, 1597–1599. [CrossRef] [PubMed]
58. Lin, C.F.; Ni, C.L.; Huang, Y.L.; Sheu, S.J.; Chen, C.C. Lignans and anthraquinones from the fruits of
Morinda citrifolia. Nat. Prod. Res. 2007, 21, 1199–1204. [CrossRef] [PubMed]
59. Su, B.N.; Pawlus, A.D.; Jung, H.A.; Keller, W.J.; McLaughlin, J.L.; Kinghorn, A.D. Chemical constituents of
Morinda citrifolia (noni) and their antioxidant activity. J. Nat. Prod. 2005, 68, 592–595. [CrossRef] [PubMed]
60. Kamiya, K.; Tanaka, Y.; Endang, H.; Umar, M.; Satake, T. Chemical constituents of Morinda citrifolia
fruits inhibit cooper-induced-low-density lipoprotein oxidation. J. Agric. Food Chem. 2004, 52, 5843–5848.
[CrossRef] [PubMed]
61. Nguyen, P.H.; Yang, J.L.; Uddin, M.N.; Park, S.L.; Lim, S.I.; Jung, D.W.; Williams, D.R.; Oh, W.K. Protein
Tyrosine Phosphatase 1b (PTP1b) inhibitors from Morinda citrifolia (noni) and their insulin mimetic activity.
J. Nat. Prod. 2013, 76, 2080–2087. [CrossRef] [PubMed]
Nutrients 2017, 9, 540 24 of 29
62. Zhang, W.Y.; Lee, J.J.; Kim, Y.; Kim, I.S.; Park, J.S.; Myung, C.S. Amelioration of insulin resistance by
scopoletin in high-glucose-induced, insulin-resistant Hepg2 cells. Horm. Metab. Res. 2010, 42, 930–935.
[CrossRef] [PubMed]
63. Shenoy, J.P.; Pai, P.G.; Shoeb, A.; Gokul, P.; Kulkarni, A.; Kotian, M.S. An evaluation of diuretic activity of
Morinda citrifolia (Linn) (noni) fruit juice in normal rats. Int. J. Pharm. Pharm. Sci. 2011, 3, 119–121.
64. Awang, D.V.C. Tyler’s Herbs of Choice: The Therapeutic Use of Phytomedicinals, 3rd ed.; Taylor & Francis Group:
Boca Raton, FL, USA, 2009.
65. Hook, I.; McGee, A.; Henman, M. Evaluation of dandelion for diuretic activity and variation in potassium
content. Pharm. Biol. 1993, 31, 29–34. [CrossRef]
66. Sang, S.; Wang, M.; He, K.; Liu, G.; Dong, Z.; Badmaev, V.; Zheng, Q.Y.; Ghai, G.; Rosen, R.T.; Ho, C.-T.
Chemical components in noni fruits and leaves (Morinda citrifolia L.). ACS Symp. Ser. 2001, 803, 134–150.
67. Jayaprakasam, B.; Olson, L.K.; Schutzki, R.E.; Tai, M.H.; Nair, M.G. Amelioration of obesity and glucose
intolerance in high-fat-fed C57Bl/6 mice by anthocyanins and ursolic acid in cornelian cherry (Cornus mas).
J. Agric. Food Chem. 2006, 54, 243–248. [CrossRef] [PubMed]
68. Kunkel, S.D.; Elmore, C.J.; Bongers, K.S.; Ebert, S.M.; Fox, D.K.; Dyle, M.C.; Bullard, S.A.; Adams, C.M.
Ursolic acid increases skeletal muscle and brown fat and decreases diet-induced obesity, glucose intolerance
and fatty liver disease. PLoS ONE 2012, 7, e39332. [CrossRef] [PubMed]
69. Norberg, A.; Hoa, N.K.; Liepinsh, E.; Van Phan, D.; Thuan, N.D.; Jornvall, H.; Sillard, R.; Ostenson, C.G.
A novel insulin-releasing substance, phanoside, from the plant Gynostemma pentaphyllum. J. Biol. Chem. 2004,
279, 41361–41367. [CrossRef] [PubMed]
70. Senthil, N.; Balu, P.M.; Murugesan, K. Antihyperglycemic effect of spirulina, insulin and Morinda citrifolia
against streptozotocin induced diabetic rats. Int. J. Curr. Microbiol. Appl. Sci. 2013, 2, 537–559.
71. Mahadeva Rao, U.S.; Subramanian, S. Biochemical evaluation of antihyperglycemic and antioxidative effects
of Morinda citrifolia fruit extract studied in streptozotocin-induced diabetic rats. Med. Chem. Res. 2008, 18,
433–446. [CrossRef]
72. Liu, Q.; Kim, S.B.; Ahn, J.H.; Hwang, B.Y.; Kim, S.Y.; Lee, M.K. Anthraquinones from Morinda officinalis roots
enhance adipocyte differentiation in 3T3-L1 cells. Nat. Prod. Res. 2012, 26, 1750–1754. [CrossRef] [PubMed]
73. Kamiya, K.; Hamabe, W.; Harada, S.; Murakami, R.; Tokuyama, S.; Satake, T. Chemical constituents of
Morinda citrifolia roots exhibit hypoglycemic effects in streptozotocin-induced diabetic mice. Biol. Pharm. Bull.
2008, 31, 935–938. [CrossRef] [PubMed]
74. Malik, E.M.; Muller, C.E. Anthraquinones as pharmacological tools and drugs. Med. Res. Rev. 2016, 36,
705–748. [CrossRef] [PubMed]
75. Teponno, R.B.; Kusari, S.; Spiteller, M. Recent advances in research on lignans and neolignans. Nat. Prod. Rep.
2016, 33, 1044–1092. [CrossRef] [PubMed]
76. Lee, S.Y.; Park, S.L.; Hwang, J.T.; Yi, S.H.; Nam, Y.D.; Lim, S.I. Anti-diabetic effect of Morinda citrifolia (noni)
fermented by Cheonggukjang in kk-a(y) diabetic mice. Evid. Based Complement. Alternat. Med. 2012, 2012.
[CrossRef] [PubMed]
77. Cargnin, S.T.; Gnoatto, S.B. Ursolic acid from apple pomace and traditional plants: A valuable triterpenoid
with functional properties. Food Chem. 2017, 220, 477–489. [CrossRef] [PubMed]
78. Wozniak, L.; Skapska, S.; Marszalek, K. Ursolic acid—a pentacyclic triterpenoid with a wide spectrum of
pharmacological activities. Molecules 2015, 20, 20614–20641. [CrossRef] [PubMed]
79. Barreto, R.S.; Albuquerque-Junior, R.L.; Araujo, A.A.; Almeida, J.R.; Santos, M.R.; Barreto, A.S.;
DeSantana, J.M.; Siqueira-Lima, P.S.; Quintans, J.S.; Quintans-Junior, L.J. A systematic review of the
wound-healing effects of monoterpenes and iridoid derivatives. Molecules 2014, 19, 846–862. [CrossRef]
[PubMed]
80. Murata, K.; Abe, Y.; Futamura-Masuda, M.; Uwaya, A.; Isami, F.; Deng, S.; Matsuda, H. Effect of
Morinda citrifolia fruit extract and its iridoid glycosides on blood fluidity. J. Nat. Med. 2014, 68, 498–504.
[CrossRef] [PubMed]
81. Sang, S.; Cheng, X.; Zhu, N.; Wang, M.; Jhoo, J.-W.; Stark, R.E.; Badmaev, V.; Ghai, G.; Rosen, R.T.; Ho, C.-T.
Iridoid glycosides from the leaves of Morinda citrifolia. J. Nat. Prod. 2001, 64, 799–800. [CrossRef] [PubMed]
82. Stadlbauer, V.; Fickert, P.; Lackner, C.; Schmerlaib, J.; Krisper, P.; Trauner, M.; Stauber, R.E. Hepatoxicity of
noni juice: Report of two cases. World J. Gastroenterol. 2005, 15, 4758–4760. [CrossRef]
Nutrients 2017, 9, 540 25 of 29
83. Shalan, N.A.A.M.; Mustapha, N.M.; Mohamed, S. Chronic toxicity evaluation of Morinda citrifolia fruit and
leaf in mice. Regul. Toxicol. Pharmacol. 2016, 83, 46–53. [CrossRef] [PubMed]
84. Inoue, K.; Yoshida, M.; Takahashi, M.; Fujimoto, H.; Shibutani, M.; Hirose, M.; Nishikawa, A. Carcinogenic
potential of alizarin and rubiadin, components of madder color, in a rat medium-term multi-organ bioassay.
Cancer Sci. 2009, 100, 2261–2267. [CrossRef] [PubMed]
85. West, B.J.; Jensen, C.J.; Westendorf, J. Noni juice is not hepatotoxic. World J. Gastroenterol. 2006, 12, 3616–3619.
[CrossRef] [PubMed]
86. Westendorf, J.; Effenberger, K.; Iznaguen, H.; Basar, S. Toxicological and analytical investigations of noni
(Morinda citrifolia) fruit juice. J. Agric. Food Chem. 2007, 55, 529–537. [CrossRef] [PubMed]
87. West, B.J.; Su, C.X.; Jensen, C.J. Hepatoxicity and subchronic tests of Morinda citrifolia (noni) fruit. J. Toxicol. Sci.
2009, 34, 581–585. [PubMed]
88. Ahmad, A.N.; Mat Daud, Z.A.; Ismail, A. Review on potential therapeutic effect of Morinda citrifolia L.
Curr. Opin. Food Sci. 2016, 8, 62–67. [CrossRef]
89. Mohd Zin, Z.; Abdul Hamid, A.; Osman, A.; Saari, N.; Misran, A. Isolation and identification of antioxidative
compound from fruit of mengkudu (Morinda citrifolia L.). Int. J. Food Prop. 2007, 10, 363–373. [CrossRef]
90. Elo, H.; Kuure, M.; Pelttari, E. Correlation of the antimicrobial activity of salicylaldehydes with broadening
of the NMR signal of the hydroxyl proton. Possible involvement of proton exchange processes in the
antimicrobial activity. Eur. J. Med. Chem. 2015, 92, 750–753. [CrossRef] [PubMed]
91. Palu, A.K.; Kim, A.H.; West, B.J.; Deng, S.; Jensen, J.; White, L. The effects of Morinda citrifolia L. (noni) on
the immune system: Its molecular mechanisms of action. J. Ethnopharmacol. 2007, 115, 502–506. [CrossRef]
[PubMed]
92. Shalan, N.A.A.M.; Mustapha, N.M.; Mohamed, S. Morinda citrifolia leaf enhanced performance by improving
angiogenesis, mitochondrial biogenesis, antioxidant, anti-inflammatory & stress responses. Food Chem. 2016,
212, 443–452.
93. Gilani, A.H.; Mandukhail, S.U.; Iqbal, J.; Yasinzai, M.; Aziz, N.; Khan, A.; Najeeb ur, R. Antispasmodic and
vasodilator activities of Morinda citrifolia root extract are mediated through blockade of voltage dependent
calcium channels. BMC Complement. Altern. Med. 2010, 10. [CrossRef] [PubMed]
94. Rosen, E.D.; Spiegelman, B.M. What we talk about when we talk about fat. Cell 2014, 156, 20–44. [CrossRef]
[PubMed]
95. Wu, J.; Bostrom, P.; Sparks, L.M.; Ye, L.; Choi, J.H.; Giang, A.H.; Khandekar, M.; Virtanen, K.A.; Nuutila, P.;
Schaart, G.; et al. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell 2012,
150, 366–376. [CrossRef] [PubMed]
96. Ouchi, N.; Parker, J.L.; Lugus, J.J.; Walsh, K. Adipokines in inflammation and metabolic disease.
Nat. Rev. Immunol. 2011, 11, 85–97. [CrossRef] [PubMed]
97. Proenca, A.R.; Sertie, R.A.; Oliveira, A.C.; Campaaa, A.B.; Caminhotto, R.O.; Chimin, P.; Lima, F.B. New
concepts in white adipose tissue physiology. Braz. J. Med. Biol. Res. 2014. [CrossRef] [PubMed]
98. Vazquez-Vela, M.E.; Torres, N.; Tovar, A.R. White adipose tissue as endocrine organ and its role in obesity.
Arch. Med. Res. 2008, 39, 715–728. [CrossRef] [PubMed]
99. Smith, U.; Kahn, B.B. Adipose tissue regulates insulin sensitivity: Role of adipogenesis, de novo lipogenesis
and novel lipids. J. Intern. Med. 2016, 280, 465–475. [CrossRef] [PubMed]
100. Cook, K.S.; Min, H.Y.; Johnson, D.; Chaplinsky, R.J.; Flier, J.S.; Hunt, C.R.; Spiegelman, B.M. Adipsin:
A circulating serine protease homolog secreted by adipose tissue and sciatic nerve. Science 1987, 237, 402–405.
[CrossRef] [PubMed]
101. Hotamisligil, G.S.; Shargill, N.S.; Spiegelman, B.M. Adipose expression of tumor necrosis factor-α: Direct
role in obesity-linked insulin resistance. Science 1994, 259, 87–91. [CrossRef]
102. Zhang, Y.Y.; Proenca, R.; Maffei, M.; Barone, M.; Leopold, L.; Friedman, J.M. Positional cloning of the mouse
obese gene and its human homolog. Nature 1994, 372, 425–432. [CrossRef] [PubMed]
103. Hu, E.; Liang, P.; Spiegelman, B.M. Adipoq is a novel adipose-specific gene dysregulated in obesity.
J. Biol. Chem. 1996, 271, 10697–10703. [PubMed]
104. Lago, F.; Dieguez, C.; Gomez-Reino, J.; Gualillo, O. The emerging role of adipokines as mediators of
inflammation and immune responses. Cytokine Growth Factor Rev. 2007, 18, 313–325. [CrossRef] [PubMed]
105. Makki, K.; Froguel, P.; Wolowczuk, I. Adipose tissue in obesity-related inflammation and insulin resistance:
Cells, cytokines, and chemokines. ISRN Inflamm. 2013, 2013, 139239. [CrossRef] [PubMed]
Nutrients 2017, 9, 540 26 of 29
127. Takikawa, M.; Inoue, S.; Horio, F.; Tsuda, T. Dietary anthocyanin-rich bilberry extract ameliorates
hyperglycemia and insulin sensitivity via activation of AMP-activated protein kinase in diabetic mice.
J. Nutr. 2010, 140, 527–533. [CrossRef] [PubMed]
128. Viollet, B.; Foretz, M.; Guigas, B.; Horman, S.; Dentin, R.; Bertrand, L.; Hue, L.; Andreelli, F. Activation
of AMP-activated protein kinase in the liver: A new strategy for the management of metabolic hepatic
disorders. J. Physiol. 2006, 574, 41–53. [CrossRef] [PubMed]
129. Nolan, J.J.; Ludvik, B.; Beerdsen, P.; Joyce, M.; Olefsky, J. Improvement in glucose tolerance and insulin
resistance in obese subjects treated with trioglitazone. N. Engl. J. Med. 1994, 331, 1188–1193. [CrossRef]
[PubMed]
130. Ferré, P. The biology of peroxisome proliferator-activated receptors: Relationship with lipid metabolism and
insulin sensitivity. Diabetes 2004, 53, S43–S50. [CrossRef] [PubMed]
131. Nayak, B.S.; Isitor, G.N.; Maxwell, A.; Bhogadi, V.; Ramdath, D.D. Wound-healing activity of Morinda citrifolia
fruit juice on diabetes-induced rats. J. Wound Care 2007, 16, 83–86. [CrossRef] [PubMed]
132. Nayak, B.S.; Marshall, J.R.; Isitor, G.; Adogwa, A. Hypoglycemic and hepatoprotective activity of fermented
fruit juice of Morinda citrifolia (noni) in diabetic rats. Evid. Based Complement. Alternat. Med. 2011, 2011,
875293. [CrossRef] [PubMed]
133. Scortichini, M.; Rossi, M.P. Preliminary in vitro evaluation of the antimicrobial activity of triterpenes and
terpenoids towards Erwinia amylovora (burrill) winslow et al. J. Appl. Bacteriol. 1991, 71, 109–112. [CrossRef]
134. Chen, J.; Li, W.L.; Wu, J.L.; Ren, B.R.; Zhang, H.Q. Hypoglycemic effects of a sesquiterpene glycoside
isolated from leaves of loquat (Eriobotrya japonica (Thunb.) Lindl.). Phytomedicine 2008, 15, 98–102. [CrossRef]
[PubMed]
135. De Tommasi, N.; De Simone, F.; Cirino, G.; Cicala, C.; Pizza, C. Hypoglycemic effects of sesquiterpene
glycosides and polyhydroxylated triterpenoids of Eriobotrya japonica. Planta Med. 1991, 57, 414–416.
[CrossRef] [PubMed]
136. Horsfall, A.U.; Olabiyi, O.; Aiyegbusi, A.; Noronha, C.C.; Okanlawon, A.O. Morinda citrifolia fruit juice
augments insulin action in sprague-dawley rats with experimentally induced diabetes. Niger. Q. J. Hosp. Med.
2008, 18, 162–165. [CrossRef]
137. Bechmann, L.P.; Hannivoort, R.A.; Gerken, G.; Hotamisligil, G.S.; Trauner, M.; Canbay, A. The interaction of
hepatic lipid and glucose metabolism in liver diseases. J. Hepatol. 2012, 56, 952–964. [CrossRef] [PubMed]
138. Quresh, K.; Abrams, G.A. Metabolic liver disease of obesity and role of adipose tissue in the pathogenesis of
nonalcoholic fatty liver disease. World J. Gastroenterol. 2007, 13, 3540–3553. [CrossRef]
139. Younossi, Z.M.; Koenig, A.B.; Abdelatif, D.; Fazel, Y.; Henry, L.; Wymer, M. Global epidemiology of
nonalcoholic fatty liver disease-meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology
2016, 64, 73–84. [CrossRef] [PubMed]
140. Smits, M.M.; Ioannou, G.N.; Boyko, E.J.; Utzschneider, K.M. Non-alcoholic fatty liver disease as an
independent manifestation of the metabolic syndrome: Results of a U.S. national survey in three ethnic
groups. J. Gastroenterol. Hepatol. 2013, 28, 664–670. [CrossRef] [PubMed]
141. Woo Baidal, J.A.; Lavine, J.E. The intersection of nonalcoholic fatty liver disease and obesity. Sci. Transl. Med.
2016, 8, 323rv321. [CrossRef] [PubMed]
142. Joshi, R.; Gangabhagirathi, R.; Venu, S.; Adhikari, S.; Mukherjee, T. Antioxidant activity and free radical
scavenging reactions of gentisic acid: In Vitro and pulse radiolysis studies. Free Radic. Res. 2012, 46, 11–20.
[CrossRef] [PubMed]
143. Pari, L.; Karthikesan, K.; Menon, V.P. Comparative and combined effect of chlorogenic acid and
tetrahydrocurcumin on antioxidant disparities in chemical induced experimental diabetes. Mol. Cell Biochem.
2010, 341, 109–117. [CrossRef] [PubMed]
144. Huang, G.J.; Liao, J.C.; Chiu, C.S.; Huang, S.S.; Lin, T.H.; Deng, J.S. Anti-inflammatory activities of aqueous
extract of Mesona procumbens in experimental mice. J. Sci. Food Agric. 2012, 92, 1186–1193. [CrossRef]
[PubMed]
145. Cho, A.S.; Jeon, S.M.; Kim, M.J.; Yeo, J.; Seo, K.I.; Choi, M.S.; Lee, M.K. Chlorogenic acid exhibits anti-obesity
property and improves lipid metabolism in high-fat diet-induced-obese mice. Food Chem. Toxicol. 2010, 48,
937–943. [CrossRef] [PubMed]
146. Huang, T.W.; Chang, C.L.; Kao, E.S.; Lin, J.H. Effect of Hibiscus sabdariffa extract on high fat diet-induced
obesity and liver damage in hamsters. Food Nutr. Res. 2015, 59, 29018. [CrossRef] [PubMed]
Nutrients 2017, 9, 540 28 of 29
147. Ham, J.R.; Lee, H.-I.; Choi, R.-Y.; Sim, M.-O.; Choi, M.-S.; Kwon, E.-Y.; Yun, K.W.; Kim, M.-J.; Lee, M.-K.
Anti-obesity and anti-hepatosteatosis effects of dietary scopoletin in high-fat diet fed mice. J. Funct. Food
2016, 25, 433–446. [CrossRef]
148. Hurtubise, J.; McLellan, K.; Durr, K.; Onasanya, O.; Nwabuko, D.; Ndisang, J.F. The different facets of
dyslipidemia and hypertension in atherosclerosis. Curr. Atheroscler. Rep. 2016, 18, 82. [CrossRef] [PubMed]
149. Atabek, M.E.; Akyurek, N.; Eklioglu, B.S.; Alp, H. Impaired systolic blood dipping and nocturnal
hypertension: An independent predictor of carotid intima-media thickness in type 1 diabetic patients.
J. Diabetes Complicat. 2014, 28, 51–55. [CrossRef] [PubMed]
150. Pan, W.H.; Bai, C.H.; Chen, J.R.; Chiu, H.C. Associations between carotid atherosclerosis and high factor VIII
activity, dyslipidemia, and hypertension. Stroke 1997, 28, 88–94. [CrossRef] [PubMed]
151. Reina, S.A.; Llabre, M.M.; Allison, M.A.; Wilkins, J.T.; Mendez, A.J.; Arnan, M.K.; Schneiderman, N.;
Sacco, R.L.; Carnethon, M.; Delaney, J.A. HDL cholesterol and stroke risk: The multi-ethnic study of
atherosclerosis. Atherosclerosis 2015, 243, 314–319. [CrossRef] [PubMed]
152. Montserrat-de la Paz, S.; Bermudez, B.; Cardelo, M.P.; Lopez, S.; Abia, R.; Muriana, F.J. Olive oil and
postprandial hyperlipidemia: Implications for atherosclerosis and metabolic syndrome. Food Funct. 2016, 7,
4734–4744. [CrossRef] [PubMed]
153. Grundy, S.M.; Cleeman, J.I.; Merz, C.N.; Brewer, H.B., Jr.; Clark, L.T.; Hunninghake, D.B.; Pasternak, R.C.;
Smith, S.C., Jr.; Stone, N.J.; National Heart, L.; et al. Implications of recent clinical trials for the national
cholesterol education program adult treatment panel III guidelines. Circulation 2004, 110, 227–239. [CrossRef]
[PubMed]
154. Mandukhail, S.U.; Aziz, N.; Gilani, A.H. Studies on antidyslipidemic effects of Morinda citrifolia (noni) fruit,
leaves and root extracts. Lipids Health Dis. 2010, 9, 88. [CrossRef] [PubMed]
155. Ojewole, J.A.O.; Adesina, S.K. Mechanism of the hypotensive effect of scopoletin isolated from the fruit of
Tetrapleura tetraptera. Planta Med. 1983, 49, 46–50. [CrossRef] [PubMed]
156. Kumar, R.; Kumar, A.; Sharma, R.; Baruwa, A. Pharmacological review on natural ACE inhibitors.
Der Pharm. Lett. 2010, 2, 273–293.
157. Ghayur, M.N.; Gilani, A.H. Ginger lowers blood pressure through blockade of voltage-dependent calcium
channels. J. Cardiovasc. Pharmacol. 2005, 45, 74–80. [CrossRef] [PubMed]
158. Schroeder, B.O.; Backhed, F. Signals from the gut microbiota to distant organs in physiology and disease.
Nat. Med. 2016, 22, 1079–1089. [CrossRef] [PubMed]
159. Clemente, J.C.; Ursell, L.K.; Parfrey, L.W.; Knight, R. The impact of the gut microbiota on human health:
An integrative view. Cell 2012, 148, 1258–1270. [CrossRef] [PubMed]
160. Li, D.; Wang, P.; Wang, P.; Hu, X.; Chen, F. The gut microbiota: A treasure for human health. Biotechnol. Adv.
2016, 34, 1210–1224. [CrossRef] [PubMed]
161. Wang, C.Y.; Ng, C.C.; Su, H.; Tzeng, W.S.; Shyu, Y.T. Probiotic potential of noni juice fermented with lactic
acid bacteria and Bifidobacteria. Int. J. Food Sci. Nutr. 2009, 60 (Suppl. 6), 98–106. [CrossRef] [PubMed]
162. Huang, H.L.; Liu, C.T.; Chou, M.C.; Ko, C.H.; Wang, C.K. Noni (Morinda citrifolia L.) fruit extracts improve
colon microflora and exert anti-inflammatory activities in Caco-2 cells. J. Med. Food 2015, 18, 663–676.
[CrossRef] [PubMed]
163. Kurniawan, D.; Widodo, E.; Djunaidi, I.H. The effect of noni (Morinda citrifolia) fruit meal as feed additive on
intestinal microfloras and villi characteristics of hybrid duck. Bul. Peternak. 2016, 40, 34–39.
164. El Kaoutari, A.; Armougom, F.; Gordon, J.I.; Raoult, D.; Henrissat, B. The abundance and variety of
carbohydrate-active enzymes in the human gut microbiota. Nat. Rev. Microbiol. 2013, 11, 497–504. [CrossRef]
[PubMed]
165. Bui, A.K.; Bacic, A.; Pettolino, F. Polysaccharide composition of the fruit juice of Morinda citrifolia (noni).
Phytochemistry 2006, 67, 1271–1275. [CrossRef] [PubMed]
166. Huang, H.L.; Ko, C.H.; Yan, Y.Y.; Wang, C.K. Antiadhesion and anti-inflammation effects of noni
(Morinda citrifolia) fruit extracts on AGS cells during Helicobacter pylori infection. J. Agric. Food Chem.
2014, 62, 2374–2383. [CrossRef] [PubMed]
Nutrients 2017, 9, 540 29 of 29
167. Howell, A.B.; Reed, J.D.; Krueger, C.G.; Winterbottom, R.; Cunningham, D.G.; Leahy, M. A-type cranberry
proanthocyanidins and uropathogenic bacterial anti-adhesion activity. Phytochemistry 2005, 66, 2281–2291.
[CrossRef] [PubMed]
168. Ghazanfari, S.; Morabi, M.A.; Bardzardi, M.M. Intestinal morphology and microbiology of broiler chicken
fed diets containing myrtle (Myrtus communis) essential oil supplementation. J. Appl. Anim. Sci. 2014, 4,
549–554.
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).