Biology Exam - Paper 6 Revision Notes: Enzyme Activity Lab
Biology Exam - Paper 6 Revision Notes: Enzyme Activity Lab
Biology Exam - Paper 6 Revision Notes: Enzyme Activity Lab
Chemical tests:
-starch: add a few drops of iodine solution, a positive result (i.e. starch is present) is a deep blue-black colour, a
negative result is orange.
-reducing sugars (e.g. glucose): Benedict’s reagent, then the mixture is heated for 2 to 3 minutes. Positive result is
an orange/brick-red colour, negative result is blue (the colour of the Benedict’s reagent).
-proteins: add a few drops of Biuret reagent, a positive result is a mauve/purple colour.
-fats: the emulsion test: ethanol is added to the mixture, this is poured into a test tube with an equal number of
distilled water, a positive result a milky-white emulsion forms.
Carbon dioxide:
Required materials
2 Potted plants
2 Bell-jars
A Candle
Petroleum jelly
Glass sheets
4. On one glass sheet, along with the potted plant place a burning candle.
7. Leave undisturbed for a few hours and test the leaves from each pot for the presence of starch.
Note
Potted plants must ideally have been kept in the dark to make the leaves starch-free before including them in
this experiment.
Potato strips are placed in solutions of different concentrations (you have to know the concentrations).
They are measured in mass before and after being in the solutions. The solution in which the potato
strip does not change in mass is the isotonic solution, but it is unlikely that the solution will be exactly
the right concentration, so you have to plot a graph and find what the concentration is. Concentration
on the x-axis and mass change in the y-axis (note: the line will go above and below the x-axis since
some of the potato strips will shrink). Where the line intersects the x-axis, there is no mass change so
that is the concentration inside the potato.
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