Professional Documents
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Microbial Enzymes
Microbial Enzymes
DOI 10.1007/s13205-016-0485-8
REVIEW ARTICLE
Received: 8 April 2016 / Accepted: 1 August 2016 / Published online: 19 August 2016
Ó The Author(s) 2016. This article is published with open access at Springerlink.com
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and are used as an alternative to hazardous chemical pol- reaction catalyzed, the enzyme commission has classified
lutant owing to their biodegradable and nontoxic nature the enzymes into six main classes, mentioned in Table 1.
(Mojsov 2011; Illanes et al. 2012; Choi et al. 2015). In Microorganisms are favored sources for industrial
addition to advantages of enzymes over conventional enzymes due to easy availability, and fast growth rate. Genetic
methods, there are some drawbacks of using enzymes in changes using recombinant DNA technology can easily be
healthcare and other industries. For many mammalian done on microbial cells for elevated enzyme production and
enzymes, 37°C and 7.4 are the optimal temperature and scientific development (Illanes et al. 2012). Production of
pH, respectively, and their activity is highly sensitive to microbial enzymes is a necessary event in the industrial sec-
any change in these parameters. Higher temperature tors, due to the high and superior performances of enzymes
([40°C), and a large deviation from the physiological pH from different microbes, which work well under a wide range
(7.4) lead to their denaturation, which limits the use of of varied physical and chemical conditions. Further, microbial
these macromolecules in non-physiological conditions. enzymes are used in the treatment of health disorders associ-
Additionally, they are susceptible to substrate or product ated with deficiency of human enzymes caused by genetic
inhibition and their products may cause allergic reactions. problems (Vellard 2003; Anbu et al. 2015). For instance,
The high cost of isolation and purification of enzymes and patients with inherited congenital sucrase-isomaltase defi-
their difficult recovery for subsequent reuse may discour- ciency are unable to digest sucrose, and therefore, sacrosidase
age their use (Johannes et al. 2006). (b-fructofuranoside fructohydrolase, EC 3.2.1.26) enzyme is
Enzymes are large macromolecules composed of poly- given orally to facilitate digestion of sucrose (Treem et al.
mers of amino acids connected by amide bonds, ranging 1999). In addition, phenylalanine ammonia lyase (EC
from kilodalton (insulin) to megadalton (ribosome) in 4.3.1.24) is used to degrade phenylalanine in genetic
molecular mass. Catalytic site of these macromolecules is phenylketonuria disorder (Sarkissian et al. 1999).
often buried deep within hydrophobic pockets, which The extensive application of microbes in different bio-
determines the specificity for their substrate. This speci- process is used to deliver a variety of products in applied
ficity of enzyme to catalyze reactions between one types of industries. Table 2 summarizes several applications of
chemical compound over the other provides the basis of its microorganisms to deliver a variety of products. The
classification and name. With the great advancement Schematic representation of industrial production of
achieved in the area of biochemistry after 1940, a large microbial enzymes has been shown in Fig. 1.
number of enzymes were isolated and characterized, and Enzymes, particularly of microbial origin, can be cul-
therefore, it was necessary to regulate the enzyme tured largely by gene manipulations, as per the need for
nomenclature. Thus, International Union of Biochemistry industrial applications. Applications of microbial enzymes
and Molecular Biology (IUBMB) in consultation with in food, pharmaceutical, textile, paper, leather, and other
International Union for Pure and Applied Chemistry industries are numerous and increasing rapidly over con-
(IUPAC) established an Enzyme Commission (EC) to be in ventional methods due to less harm to the environment,
charge of guiding the naming and systematic classification greater efficiency, and the higher quality products (Jordon
for enzymes (Liese et al. 2006). According to the type of 1929; Kamini et al. 1999; Gurung et al. 2013).
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Table 2 continued
Industry Enzyme Function Microorganisms
In this review, the attentions are given to present a Pharmaceutical and analytical industry
succinct role of all microbial enzymes involved in various
fields of technical applications, such as feed industry, food Enzymes have many significant and vital roles in the
processing and cosmetics. Furthermore, efforts are made to pharmaceutical and diagnostic industries. These are
draw a simple and clear scenario about the industrial extensively used as therapeutic drugs in health issues
structure of global enzyme market. associated with enzymatic deficiency and digestive disor-
ders, and in diagnostic procedures such as ELISA and
diabetes testing kits (Mane and Tale 2015).
The use of enzymes in industrial processes Enzyme applications in medicine are as extensive as in
industry and are growing rapidly. At present, most
Enzymes are used in industrial processes, such as baking, prominent medical uses of microbial enzymes are removal
brewing, detergents, fermented products, pharmaceuticals, of dead skin, and burns by proteolytic enzymes, and clot
textiles, leather processing. Here are a range of processes busting by fibrinolytic enzymes. Nattokinase (EC
showing how enzymes are used. 3.4.21.62), a potent fibrinolytic enzyme, is a promising
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manufacturing and ingredients industry have improved the baking market. The baking enzymes industry is expected to
basic processes to provide better markets with safer and reach $695.1 million by 2019 growing at a CAGR 8.2 %
higher quality products. Furthermore, the enzymes gained from 2013 to 2019 (2014a: Baking Enzymes Market).
interest in new areas such as fat modification and sweetener Bread making is one of the most common food processing
technology (Li et al. 2012). Currently, the food and bev- techniques globally. The use of enzymes in bread manu-
erage segment dominated the industrial enzyme market and facturing shows their value in quality control and efficiency
it is projected to reach a value of $2.3 billion by 2020 of production. Amylase, alone or in combination with other
(2015c: Industrial Enzymes Market). In beverage and food enzymes, is added to the bread flour for retaining the mois-
industry, enzymes are added to control the brewing process ture more efficiently to increase softness, freshness and shelf
and produce consistent, high-quality beer; to enhance the life. Additionally, lipase and xylanase (EC 3.2.1.8) are used
functional and nutritional properties of animal and veg- for dough stability and conditioning while glucose oxidase
etables proteins by the enzymatic hydrolysis of proteins, and lipoxygenase added to improve dough strengthening and
for higher juice yield with improved color and aroma. whiteness. Transglutaminase (EC 2.3.2.13) is used in baking
The application of enzymes in food industry is seg- industry to enhance the quality of flour, the amount and
mented into different sectors, such as baking, dairy, juice texture of bread and the texture of cooked pasta (Kuraishi
production and brewing. Worldwide, microbial enzymes et al. 1997; Moore et al. 2006; Kieliszek and Misiewicz
are efficiently utilized in bakery—the principal application 2014). Lipases are also used to improve the flavor content of
market in food industry—to improve dough stability, bakery products by liberating short-chain fatty acids through
crumb softness and structure, and shelf life of products. esterification and to prolong the shelf life of the bakery
Increased uses of microbial enzymes in cheese processing products (Andreu et al. 1999; Dauter et al. 1999; Monfort
are largely responsible for the use of enzymes in dairy et al. 1999; Collar et al. 2000; Kirk et al. 2002; Fernandes
industry, which is the next largest application industry 2010; Li et al. 2012; Adrio and Demain 2014).
followed by the beverages industry.
Dairy industry
Baking industry
Dairy enzymes, an important segment of food enzyme
Baking enzymes are used for providing flour enhancement, industry, are used for the development and enhancing
dough stability, improving texture, volume and color, organoleptic characteristics (aroma, flavor and color) and
prolonging crumb softness, uniform crumb structure and higher yield of milk products. The use of enzymes (pro-
prolonging freshness of bread. To meet rising demand for teases, lipases, esterases, lactase, aminopeptidase, lyso-
quality, enzymes are seen as natural solutions in today’s zyme, lactoperoxidase, transglutaminase, catalase, etc.) in
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dairy market is well recognized and varies from coagulant by the addition of enzymes. Enzymes digest pectin, starch,
to bio-protective enzyme to enhance the shelf life and proteins and cellulose of fruits and vegetables and facilitate
safety of dairy products. Dairy enzymes are used for the improved yields, shortening of processing time and
production of cheese, yogurt and other milk products (Pai enhancing sensory characteristics (Mojsov 2012). Amy-
2003; Qureshi et al. 2015). lases are used for clarification of juices to maximize the
Rennet, a combination of chymosin and pepsin, is used production of clear or cloudy juice (Vaillant et al. 2001;
for coagulation of milk into solid curds for cheese pro- Sivaramakrishnan et al. 2006). Cellulases and pectinases
duction and liquid whey. Currently, approximately 33 % of are used to improve extraction, yield, cloud stability and
global demand of cheese produced using microbial rennet. texture in juices (Bhat 2000; Kashyap et al. 2001; Garg
Other proteases find applications for accelerated cheese et al. 2016). Naringinase (EC 3.2.1.40) and limoninase,
processing and in reduction of allergenic properties of milk debittering enzymes, hydrolyze bitter components and
products (Qureshi et al. 2015). Currently, lipases are improves the quality attributes of citrus juices (Hotchkis
involved in flavor improvement, faster cheese preparation, and Soares 2000; Li et al. 2012). Pectin, a structural
production of customized milk products, and lipolysis of heteropolysaccharide, present in nearly all fruits is required
milk fat (Sharma et al. 2001; Ghosh et al. 1996). Transg- to be maintained to regulate cloudiness of juices by poly-
lutaminase catalyzes polymerization of milk proteins and galacturonase (EC 3.2.1.15), pectin esterases (EC 3.1.1.11),
improves the functional properties of dairy products (Rossa pectin lyase (EC 4.2.2.10) and various arabanases
et al. 2011; Kieliszek and Misiewicz 2014). (Kashyap et al. 2001; Yadav et al. 2001).
Lactose intolerance is the lack of ability of human being Microbial amylases may be utilized in the distilled alco-
to digest lactose due to deficiency of lactase enzyme. holic beverages to hydrolyze starch to sugars prior to fer-
Lactase (b-galactosidase, EC 3.2.1.23) catalyzes hydrolysis mentation and to minimize or remove turbidities due to starch.
of lactose to glucose and galactose, and therefore, is used The application of enzymes to hydrolyze unmalted barley and
as a digestive aid and to enhance the solubility and other starchy adjuncts facilitate in cost reduction of beer
sweetness in milk products (Soares et al. 2012; Qureshi brewing. In brewing, development of chill-hazes in beer may
et al. 2015). It is required to minimize or removal of lactose be control by the addition of proteases (Okafor 2007).
content of milk products for lactose-intolerant people to
prevent severe tissue dehydration, diarrhea, and sometimes Feed industry
fatal consequences (Kardel et al. 1995; Pivarnik et al. 1995;
Mahoney 1997). To meet the continuously increasing worldwide demand of
milk and meat consumption, growth of feed enzymes
Beverages industry occurred steadily. The use of enzymes in animal diets
initiated in the 1980s and exploded in the 1990s. Feed
The beverage industry is divided into two major groups and enzymes are gaining importance as they can increase the
eight sub-groups. The nonalcoholic group contains soft digestibility of nutrients and higher feed utilization by
drink and syrup, packaged water, fruit juices along with tea animals (Choct 2006). The global market for feed enzymes
and coffee industry. Alcoholic group comprised distilled was estimated $899.19 million in 2014 and expected to
spirits, wine and beer (Encyclopedia of Occupational reach nearly $1.3 billion by 2020, at a CAGR of 7.3 %
health and safety). Industrial enzymes are used in breweries from 2015 to 2020 (2015a: Feed Enzyme Market).
as processing aids and to produce consistent and high- Feed enzymes may be used in animal diet formulation.
quality products. In the brewing industries, microbial For instance, these are added to degrade specific feed
enzymes are used to digest cell wall during extraction of components which are otherwise harmful or no nutritional
plant material to provide improved yield, color, and aroma value. In addition, the protein dietary value of feeds
and clearer products (Kårlund et al. 2014). available for poultry may also be enhanced by the appli-
The enzyme applications are an integrating ingredient of cation of feed enzymes (Collection of information on
the current fruit and vegetable juice industry. Enzymes are enzymes 2002). Feed enzymes mainly used for poultry are
used in fruit and vegetable juice industry as processing aids phytases, proteases, a-galactosidases, glucanases, xyla-
to increase the efficiency of operation, for instance, peel- nases, a-amylases, and polygalacturonases (Walsh et al.
ing, juicing, clarification, extraction and improve the pro- 1993; Chesson 1993; Bhat 2000; Adrio and Demain 2014).
duct quality (Law 2002). Application of cellulases, The phytase, largest enzyme segment in the feed industry,
amylases, and pectinases during fruit juice processing for is used to utilize natural phosphorous bound in phytic acid
maceration, liquefaction, and clarification, improve yield in cereal-based feed (Lei and Stahl 2000; Bhat 2000; Frias
and cost effectiveness (Kumar 2015; Garg et al. 2016). The et al. 2003). Monogastric animals are unable to digest plant
quality and stability of juices manufactured are enhanced based feeds containing high amount of cellulose and
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hemicelluloses. Xylanase and b-glucanase are added to utilization of enzymes reduce processing time, energy
their feeds as these enzymes fully degrade and digest high consumption and amount of chemicals in processing.
amount of starch (Bhat 2000; Kirk et al. 2002). Proteases Enzymes are also used to enhance deinking, and bleach in
are also used in animal feeds to overcome anti-nutritional paper and pulp industry and waste treatment by increasing
factors by degrading proteins into their constituent amino biological oxygen demand (BOD) and chemical oxygen
acids. Apart from improving the nutritional value of feed demand (COD) (Srivastava and Singh 2015). Xylanases
for better feed conversion by the animals, these feed and ligninases are used in paper and pulp industries to
enzymes are gaining importance for their role in feed cost augment the value of the pulp by removing lignin and
reduction and meat quality improvement (Lei and Stahl hemicelluloses (Maijala et al. 2008). In these industries,
2001; Adrio and Demain 2014). amylases uses include starch coating, deinking, improving
paper cleanliness and drainage improvement (Kuhad et al.
Polymer industry 2011). Lipases are employed for deinking and enhancing
pitch control while cellulases are used for deinking,
To meet the increased consumption of polymers and the improving softness and drainage improvement (Kirk and
growing concern for human health and environmental Jeffries1996). Cellulase has also been used for the devel-
safety has led to the utilization of microbial enzymes for opment of the bioprocess for recycling of used printed
synthesis of biodegradable polymer. In vitro enzyme cat- papers (Patrick 2004). The application of laccase is an
alyzed synthesis of polymer is an environmental safe pro- alternative to usage and requirement of large amount of
cess having several advantages over conventional chemical chlorine in chemical pulping process; subsequently, reduce
methods (Vroman and Tighzert 2009; Kadokawa and the waste quantity that causes ozone depletion and acidi-
Kobayashi 2010). Biopolymers are environmentally fication (Fu et al. 2005). Moreover, mannases are used for
friendly materials as these are synthesized from renewable degrading glucomannan to improve brightness in paper
carbon sources via biological processes, degrade biologi- industry (Clarke et al. 2000).
cally after use and return to the natural environment as
renewable resources, such as CO2 and biomass (Hiraishi Leather industry
and Taguchi 2009). Biopolymers, such as polyesters,
polycarbonates and polyphosphates are used in various The leather industry is more customary, and therefore,
biomedical applications, e.g., orthopedic devices, tissue discharges and waste disposed from different stages of
engineering, adhesion barriers, control drug delivery, etc. leather processing are causing severe health hazards and
(Gunatillake and Adhikari 2003; Ulery et al. 2011). environmental problems (Choudhary et al. 2004). The
The biopolymers market is growing at a CAGR of 14.5 % biodegradable enzymes are efficient alternative to improve
due to high penetration of materials in industries like medi- the quality of leather and help to shrink waste (Adrio and
cal, packaging, appliances, automotive, electronics, and Demain 2014). The initial attempt for application of
furniture and the market is expected to reach nearly $3.6 enzyme in leather industry was made for dehairing process,
billion by 2018 ( 2014b: Bioplastics & Biopolymers Market). the largest process in leather preparation which require
Increasing demand of packaging materials and environ- bulk amount of enzymes like proteases, lipases and amy-
mental safety can be addressed by the biodegradable poly- lases (Sankaran 1995; Bailey 1992; Raju et al. 1996).
mers. Lipases catalyze the polymerization of lactones, cyclic Enzymatic dehairing applications are attractive because it
diesters and cyclic carbonates to produce polyesters or can preserve the hair and contribute to fall in the organic
polycarbonates (Kobayashi 2010). Lipase catalyzed poly- load released into the effluent. Enzymatic dehairing pro-
merization is an eco-friendly technique for the preparation of cesses minimize or eliminate the dependence on harmful
useful polyesters by polycondensation as well as poly-ad- chemicals, such as sulfide, lime and amines (Green 1952;
dition reactions (Vroman and Tighzert 2009). The other Money 1996; de-Souza and Gutterres 2012).
enzymes involved in biopolymer industries are laccase (EC Enzymes are required for facilitating procedure and
1.10.3.2), peroxidase and transglutaminase for forming enhancing leather quality during different stages in leather
cross-links in biopolymers to produce materials in situ by processing, such as, curing, soaking, liming, dehairing,
means of polymerization processes (Gurung et al. 2013). bating, picking, degreasing and tanning (Mojsov 2011).
The enzymes used in leather industries are alkaline pro-
Paper and Pulp industry teases, neutral proteases, and lipases. Alkaline proteases
are used to remove non fibrillar proteins during soaking, in
With increasing awareness of sustainability issues, uses of bating to make leather soft, supple and pliable. Neutral and
microbial enzymes in paper and pulp industry have grown alkaline proteases, both are used in dehairing to reduce
steadily to reduce adverse effect on ecosystem. The water wastage (Rao et al. 1998). In addition to this, lipases
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are used during degreasing to remove fats (Choudhary et al. dismutase (SOD, EC 1.15.1.1) is used to arrest free radicals
2004; Sharma et al. 2001). The advantages of using and to control damage to skin caused by air and water
enzymes instead of chemicals in liming are stainless pelt, pollutions, microbes and other harmful factors. SOD and
reduced odor, low BOD and COD in effluents, and peroxidases are used in combination in sunscreen cream as
improved hair recovery (Bhatia 2003). free radical scavengers to reduce erythema (Babizhayev
2006). Proteases are used in skin creams to clean and
Textile industry smoothen the skin through peeling off dead or damaged
skin (Cho et al. 2007).
The textile industry is responsible for vast generation of Other widely used enzymes in toothpaste and mouth-
waste from desizing of fabrics, bleaching chemicals and wash are endoglycosidase and papain, which are used to
dye is one of the largest contributors to environmental whiten teeth, to remove plaque and to remove odor-causing
pollution (Ahuja et al. 2004). In such industries, enzymes deposits on teeth and gum tissue (Buckingham 1985).
are used to allow the development of environmentally Laccase, oxidases, peroxidases, and polyphenol oxidases
friendly technologies in fiber processing and strategies to are used in hair dyeing (Lang and Cotteret 2004), lipase,
improve the final product quality (Choi et al. 2015). The catalase, papain, bromelain and subtilisin in skin care
main classes of enzymes involved in cotton pre-treatment (Diehl 2008); and protein disulfide isomerase, glutathione
and finishing processes are hydrolase and oxidoreductase. sulfhydryl oxidase and transglutaminase in hair waving (Li
The group of hydrolase includes amylase, cellulase, cuti- et al. 2012). Additionally, enzymes are also used in contact
nase, protease, pectinase and lipase/esterase, which are lens cleaners to remove protein films (Alfa and Jackson
involved in the biopolishing and bioscouring of fabric, anti- 2001).
felting of wool, cotton softening, denim finishing, desizing,
wool finishing, modification of synthetic fibers, etc. (Ara- Enzymes in detergents
ujo et al. 2008; Chen et al. 2013). Oxidoreductase, other
group of enzyme, includes catalase, laccase, peroxidase, Detergents represent the largest industrial application of
and ligninase, which are involved in bio-bleaching, bleach enzymes amounting to 25–30 % of the total sales of
termination, dye decolorization, fabric, wool finishing, etc. enzymes and expected to grow faster at a CAGR of about
(Mojsov 2011). A brief detail of applications of enzymes in 11.5 % from 2015 to 2020 (2014c: Global Market for
textiles industries are shown in Table 4. Enzymes in Industrial Applications). Enzymes have con-
tributed significantly to the growth and development of
Enzymes in cosmetics industrial detergents, which is a prime application area for
enzymes today. Detergents are used in miscellaneous
The applications of enzymes in cosmetics have been con- applications as dishwashing, laundering, domestic, indus-
tinuously increased. Enzymes are used as free radical trial and institutional cleaning (Schafer et al. 2002). The
scavengers in sunscreen cream, toothpaste, mouthwashes, enzymes in detergent products are used to remove protein,
hair waving and dyeing (Li et al. 2012). The superoxide starch, oil and fats based stains and to increase the
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effectiveness of detergents (Kirk et al. 2002; Hasan et al. (Gentile et al. 1992). Oxidoreductases, such as polyphenol
2010). The enzymes in laundry detergents are weight oxidase is involved in the synthesis of 3,4-dihydrox-
efficient, cleave off damaged cotton fibers, improve ylphenyl alanine (DOPA), a chemical used in the treatment
whiteness, color and fabric care. Enzymes mainly used in of Parkinson’s disease (Faber 1997). Oligosaccharides and
detergent products are of hydrolase group and currently, polysaccharides, play vital roles in cellular recognition and
most commonly used enzymes are amylase and protease. communication processes, are synthesized industrially
Sometimes a combination of enzymes, including proteases, using high regio- and stereoselectivity of glycosyltrans-
amylases, pectinases, cellulases and lipases used to ferases (Ginsburg and Robbins 1984). Lyases are involved
increase efficiency on stain cleaning and fabric care (Li in organic synthesis of cyanohydrins from ketones, acry-
et al. 2012). lamide from acrylonitrile, malic acid from fumaric acid
Amylases and lipases are effective on removing starchy (Faber 1997; Zaks 2001). The nitrile hydratase mediated
food deposits and stains resulting from fatty products, process for the production of acrylamide is carried out by
respectively (Masse et al. 2001). Cutinase (EC 3.1.1.74), a the Nitto Chemical Company of Japan at a scale of more
hydrolytic enzyme, is used as a lipolytic enzyme in dish- than 40,000 tons per year (Zaks 2001). A multi-million ton
washing and laundry detergents (Filipsen et al. 1998; Pio of high fructose corn syrup (HFCS), an alternative sweet-
and Macedo 2009). Protease digests on organic stains, such ener to sucrose in the food and beverage industry, is pro-
as grass, blood, egg and human sweat, whereas cellulases duced every year commercially using glucose isomerase
are used to brighten colors, soften fabrics and to eliminate (Gerhartz 1990).
small fibers from the fabric without damaging the major
fibers of the fabric (Hasan et al. 2010; Kuhad et al. 2011). Waste treatment
Protease and amylase are used particularly in dishwasher
detergents to remove protein and carbohydrate containing The use of enzyme for waste management is extensive and
food particles (Keshwani et al. 2015). The application of a number of enzymes are involved in the degradation of
enzymes in detergent products is advantageous as these toxic pollutants. The industrial effluents as well as
products contain less bleaching agents, phosphates, and domestic waste contain many chemical commodities,
consequently have beneficial effects on public and envi- which are hazardous or toxic to the living being and
ronmental health (Olsen and Falholt 1998; Novozyme ecosystem. Microbial enzyme(s), alone or in combinations,
2013). are used for the treatment of industrial effluents containing
phenols, aromatic amines, nitriles, etc., by degradation or
Organic synthesis industry bioconversion of toxic chemical compound(s) to innocuous
products (Klibanov et al. 1982; Raj et al. 2006; Rubilar
Enzyme based processes for production of fine chemicals et al. 2008; Pandey et al. 2011). A number of enzymes
are rapidly gaining practical significance owing to more employed for waste treatment are amidases, amylases,
economical high purity products in an eco-environmentally amyloglucosidases, cellulases, glucoamylases, lipases,
acceptable manner (Nagasawa and Yamada 1995). nitrile hydratases, pectinases and proteases (Margesin
Enzymes are preferred in industrial chemical synthesis et al.1999; Riffaldi et al. 2006; Karigar and Rao 2011). The
over conventional methods for their high selectivity, i.e., detoxification of toxic organic compounds through oxida-
chiral, positional and functional group specific (Schmid tive coupling is mediated with oxidoreductases (Karigar
et al. 2001). Such high selectivity is extremely advanta- and Rao 2011). These enzymes, like laccase, manganese
geous in chemical synthesis as it may offer several benefits peroxidase, lignin peroxidase and tyrosinase catalyze the
such as minimal or no by-product formation, easier sepa- removal of chlorinated phenolic compounds from indus-
ration, and less environmental problems. Besides, high trial effluents (Gianfreda et al. 1999; Mai et al. 2000; Have
catalytic efficiency and mild operational conditions are and Teunissen 2001; Piontek et al. 2001; Le Roes-Hill and
advantages of enzyme mediated commercial applications. Prins 2016). The microbial enzymes are also utilized to
Catalytic potential of microorganisms have been employed recycle the waste for reuse, e.g., to recover additional oil
for hundreds of years in the production of alcohol, and from oil seeds, to convert starch to sugar, to convert whey
cheese for industrial synthetic chemistry (Johannes et al. to various useful products (Kalia et al. 2001; http://www.
2006). Among the enzymes in organic synthesis, lipases are unido.org/fileadmin/import/32068_35FoodWastes). Micro-
the most frequently used, particularly, in the formation of a bial oxygenases, such as monooxygenases and dioxyge-
wide range of optically active alcohols, acids, esters, and nases have a broad substrate range, and are active against a
lactones (Jaegera and Reetz 1998; Hasan et al. 2006). wide range of compounds, including the chlorinated
Lipases are used for the production of (S, R)-2, 3-p- aliphatics (Fetzner and Lingens 1994; Arora et al. 2009).
ethoxyphenylglycyclic acid, an intermediate for diltiazem These are used in the degradation of halogenated organic
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3 Biotech (2016) 6:174 Page 11 of 15 174
compounds containing pollutants, like herbicides, insecti- Pharmaceutical enzymes cover almost 50 % of total
cides, fungicides, hydraulic and heat transfer fluids, plas- enzyme demand in India, followed by detergent enzymes
ticizers, and intermediates for chemical synthesis (Fetzner (20 %) and textile enzymes (20 %) (Binod et al. 2013).
and Lingens 1994; Karigar and Rao 2011). In 2012, industrial enzymes market globally including
the market in India was at marginal position with a net
value of around $105 million, but expected to grow sig-
Indian enzymes market nificantly with an average of C10 % per year through 2017
to reach nearly $173 (http://www.sebi.gov.in/cms/sebi_
Around the globe, enzyme market is dominated by the food data/attachdocs/1453372309087.pdf). Greater than 60 %
and beverage products, and drug industry that go directly or enzyme market in India was contributed by the multina-
indirectly for human consumption. The biggest challenge tionals companies, whereas the rest was met by the
in front of fast growing economies such as India is to domestic manufacturers. The domestic consumption of
provide food and healthcare to even their larger population. enzymes for 2011-12 stood at about $110 million, while the
India, an agriculture-based economy, is predicted to grow exports raked $32 million in revenues during this period
at 7.9 % by 2018 (http://data.worldbank.org/country/india) (Biospectrum 2012). In 2012-13, Advanced Enzymes
and an attractive market that is opening her doors for technologies Ltd., the largest manufacturer and exporter of
industrial enzyme based manufacturing sector. Indian enzyme products in India, had nearly 30 % share in the
biotech sector accounts 2 % of the global biotech market, enzymes industry and second in line after 44 % market
but it is gaining worldwide visibility due to the investment share of Denmark based Novozymes. Other prominent
opportunities as well as its research output (Binod et al. manufacturers were Rossari Biotech, Maps Enzymes,
2013). Recently, Bharat Biotech, a Hyderabad-based Lumis Biotech and Zytex (CRISIL Research 2013)
pharma company, has developed world first Zika virus (Table 5). The demand of enzymes by their type is illus-
vaccine, which is ready for pre-clinical trials (http://www. trated in Fig. 2 (CRISIL Research 2013), (http://www.sebi.
huffingtonpost.in/2016/02/07/zika-virus_0_n_9179776. gov.in/cms/sebi_data/attachdocs/1453372309087.pdf).
html), demonstrating the ‘‘Make in India’’ efforts (http://
www.makeinindia.com/sector/biotechnology).
Protease, xylanase, a-amylases, Novozymes 1983 (Indian 44 Household care, textiles, food and
glucoamylase, etc. India manufacturing beverages, oil and fats, baking,
unit) beverage alcohol etc.
Amylase, protease, phytase, xylanase, b- Advanced 1989 30 Food and beverages, pharma, animal
mannanase, a-galactosidase, etc. Enzymes feed, textiles, detergent, biofuel,
technologies etc.
Ltd.
Amylases, Proteases, Cellulases, Xylanase, MAPS 1975 Rest of the Market Textile, leather, feed, etc.
Glucoamylase, Pectinase, Catalase, Enzymes share along with
Lipase and Phytase, etc. Ltd. other manufacturers
Protease, amylase, cellulase, mannanase, Rossari biotech 1997 Textile, pharma, Food and
catalase, laccase, pectinases, etc. Beverages, feed, detergent,
chemical, etc.
Alkaline Pectinase, Amylase, cellulase, Lumisbiotech Textiles, Food and Beverages, feed,
Laccase, Catalase, lipase, protease, etc.
xylanase, b-glucanase, etc.
Protease, pectinase, amylase, xylanase Anthem 2007 paper, grain processing, beverages,
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