CO2 Incubators

Thermo Forma
CO2 Incubators
The world leader in serving science Rainer Zagst
Forma’s Contribution to Thermo Incubator Firsts

ƒ First Controlled CO2 (T/C) 3156/3324 1975

ƒ First microprocessor controls Stericult 1984

ƒ First IR Sensor Stericult 1984
ƒ First HEPA filtration
Stericult 1984
ƒ First Class 100 filtration
3110 Series 2000
ƒ First 140C Sterilization cycle
Steri-Cycle 2001

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 Thermo Incubator History

Forma’s History Thermo’s Offering 2005

ƒ 1950’s – Forma enters the incubator • Forma
business in the late 1950’s − 3110 Water Jacket,
ƒ 1960’s - Range gradually expands Stericycle, SteriCult
ƒ 1970’s-early 80’s - Era of the 3325 &
• Jouan
3326
− IGO series
ƒ 1980’s - Stericult
• Napco
ƒ 1990’s - 3110 Water Jacket
− 5400, 6100, 7100
ƒ 2002/3 – Steri-Cycle & Gen2 Steri-Cult
• Precision
ƒ 2003/4 – Jouan purchased (included
Napco)
− BOD cabinets
ƒ 2005 – Kendro purchased (Included • Heraeus
Heraeus and Revco (incl.OEMs)) − HeraCell
• Revco
− Elite, Ultima, Fisher Isotemp

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Incubator Applications

ƒ Mammalian cell culture

ƒ In-Vitro Fertilization
ƒ Stem Cell Research
ƒ Bacterial cell culture
ƒ Plant culture
ƒ Insect culture
ƒ Packaging testing
ƒ Product testing
ƒ Many more...

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 Customer Need

ƒ Stable, repeatable growth conditions

• Desire for consistent, high cell growth rates
• Fear of loss of product, resulting in lost time and money
• Product has to work
ƒ Protection from contamination
• Fear of loss of product, resulting in lost time and money
• Reduce time needed to maintain system cleanliness
ƒ Security of cultures
• Protection from misuse
• Protection from accidental loss of power
ƒ Automate drug discovery process
ƒ Broader range of solutions based on need for validation

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 CO2 Incubators –
The world leader in serving science a laboratory necessity
Incubator Technologies

Basic Elements

ƒ Heat (add or remove)

ƒ Humidity (Rh)
ƒ Gas (CO2, O2, or N2)
ƒ Airflow
ƒ Light (add or block)

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Incubator Technologies

Heating Methods:

ƒ Gravity convection
ƒ Mechanical Convection
ƒ Direct Heat (Radiant Wall)
ƒrm o
Water Jacket
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Incubator Technologies

Cooling Methods:

ƒ Cooling coil
m o
r
ƒe Mechanical refrigeration
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mo
ƒerThermoelectric
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Incubator Technologies

Humidification Methods:

ƒ Elevated
• Convection (water pan)

ƒ Controlled
• Vapor generator
• Steam generator (boiler)
• Mister (atomizer)
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T • Dryer
o (rotary bed, column filter, etc.)
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Th

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Incubator Technologies

Light Control:

ƒ Blocking
m o
her • Solid outer doors
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• Light-tight door covers

ƒ Elevated
rmo• Diurnal
e
Th • Light stability

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Incubator Technologies

Gas Injection/Control

ƒ Controlled CO2
• Infrared (IR) Control
m o
her• Thermal Conductivity (TC) Control
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T •mConstant-Flow
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ƒT O - Fuel Cell
2
• N2 injection to suppress O2
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 Thermo’s Incubators

The world leader in serving science

 LED Product Overview – CO2 Incubators

ƒ Key product segments

• Water jacket
• Forma
• Napco
• Jouan

• Direct heat
• Forma
• Heraeus

• High end
• Forma
• Heraeus

• Reach-In
• Forma

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 Ex-BTD CO2 Incubators
ƒ Forma Series II Water Jacket
• 184L
• Water Jacketed
• HEPA
ƒ Forma Direct Heat Series
• 184L
• Basic direct heat unit
• HEPA (option)

ƒ Steri-CycleTM Series
• 184L
• Direct Heat
• Sterilization
• HEPA

ƒ Steri-CultTM Series
• 232L or 332L
• Direct Heat
• Active Humidity
• 140C Sterilization
• HEPA

ƒ Forma Reach-in

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Shared Incubator Features

ƒ Chamber configuration (Steri-

Cult, Series II, Steri-Cycle, Direct
Heat):
• Large culturing space
• Polished stainless steel chamber
• Easy to clean – 100% coved corners
• Easy to clean – removable cleanable door
gasket
• Easy to maintain - all filters in the
chamber
• Interchangable interior components that
remove without tools

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Shared Incubator Features

ƒ Chamber configuration (Series II, Steri-Cycle, Direct Heat):

• More accurate and easy to service – all sensors in the chamber
• Improved air uniformity and quality through directed airflow
• Effective and easy to maintain - all filters in the chamber

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Shared Incubator Features

ƒ Control Panel (Steri-Cult, Series II,

Steri-Cycle, Direct Heat, Reach-In):
• Enviroscan Microprocessor With Message Center
• Easy to read temperature and CO2 displays
• Fully adjustable Tracking Alarms
• Electronic Calibration for CO2 (IR) and O2
• Wide range of available outputs (0-1v, 0-5v., 4-
20ma., RS485)

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 Choice of CO2 Controls

Automatic CO2
• TC-Thermal Conductivity
• IR-Infrared

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Shared Incubator Features

ƒ Convenience Features (Steri-Cult, Series

II, Steri-Cycle, Direct Heat):
• Easy to stack – all parts included
• Field reversible inner and outer door swing
• Standard convenience outlet
• Built-in 1” (2.5 cm.) access port
• Standard remote alarm contacts

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Series II 3110 CO2 Incubator

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Series II Water Jacket

THE Premium Water Jacketed Incubator

ƒ Quality construction
ƒ Advanced microprocessor controls
ƒ Exclusive 100% HEPA Filtered Class 100 Air
ƒ Exclusive heated dual-pane inner door
ƒ Stable water jacket on all 5 chamber sides
ƒ Automatic CO2 and O2 control
• Thermal Conductivity (TC) or Infrared (IR)

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Class 100 Airflow

ƒ Unit reaches Class 100 or better within 5 minutes of startup

• Directed airflow forces 100% of chamber air through filter
• Cleanroom quality air prevents particulate contamination from
landing on interior surfaces

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Class 100 Performance

Claim backed up by:

• Third party testing
• Technical White paper
• Full validation protocol
ƒ Claim is REPEATABLE and VALIDATABLE

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Construction Features

WATER
TEMP (C) JACKET

AIR
JACKET

TIME (MIN.)
Triple-Wall Water Jacket Provides Protection from:
ƒ Power outages
ƒ Power fluctuations
ƒ Changes in ambient temperature

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Easy to Clean

“Snap-fit” blower assembly

• No tools needed to remove

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Dozens of Options

Factory Installed
- Built-In Gas Guards (CO2 and N2)
- RH Readout with Alarm
- Chamber Cooling Coil
- Full range of outputs
Customer Installed
- Inner glass doors
- Copper Shelf Systems
- Independent Inner Door Kit
- Roller Base
- Floor Stand

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Extensive Warranty

ƒ One Year Parts and Labor

ƒ TC Gas Sensor - 5 Years
ƒ IR Gas Sensor - 2 Years
ƒ O2 Gas Sensor - 1 Year
ƒ LIFETIME Chamber Warranty

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Steri-Cycle™ CO2 Incubator

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Steri-Cycle™

Unique Double Threat to Contamination:

- Documented Class 100 HEPA air filtration

- Documented high heat Sterilization cycle

• PLUS

- Direct Heat
- Familiar Thermo Forma controls and construction
- Choice of T/C or IR CO2 sensors

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Continuous Contamination Control

100% HEPA filtration of chamber air

– Documented Class 100 performance
– Unit reaches Class 100 within 5 minutes
of startup
– Filter is easy to access and replace

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Class 100 Performance

Class 100 claim backed up by:

• Third party testing
• Technical White paper
• Full validation protocol
Claim is REPEATABLE and VALIDATABLE

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On-Demand Contamination Elimination

High heat sterilization cycle:

– Effective - Documented chamber STERILIZATION
– Fast - Sterilization phase in under 3 hours
– Easy - Intuitive cycle run process
– Safe - Exclusive personnel protection alarms and cool
exterior surfaces

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Documented Sterilization Effectiveness

ƒ The most heat resistant “Reference Organism” available

used to verify Sterilization process
ƒ Tests conducted by a leading sterilization scientist under
controlled conditions
ƒ Scientific white paper available

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Fast Sterilization Cycle

Total sterilization cycle time under 12 hours.

– 140C sterilization phase under 2 hours.
– Major time savings over low-temp cycles

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Easy Procedure

ƒ On-screen instructions guide you through the process

ƒ Messages alert you to current status of cycle

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Safe Procedure

ƒ Unique outer door switch and continuous alarm

ƒ Three different indicators of Sterilization activation
ƒ Password protection against unauthorized cycle initiation
ƒ Cool door and cabinet during Sterilization.

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Designed for Durability

Special heat-resistant components

– All parts tested at sterilization temperature
– Life of unit not affected by use of sterilization cycle

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Ideal Culturing Environment

ƒ Directed vertical laminar airflow

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Dozens of Options

Factory Installed
– Built-In Gas Guards (CO2 and N2)
– RH Readout with Alarm
– Full range of outputs
Customer Installed
– Inner glass doors
– Copper Shelf Systems
– Independent Inner Door Kit
– Roller Base

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Direct Heat CO2 Incubator

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310 Series Direct Heat

THE Premium Direct Heat Incubator

ƒ 184.1 liters (6.5 cu. ft.)
ƒ Quality construction
ƒ Advanced microprocessor controls
ƒ Precise direct heat on all 6 chamber sides
ƒ Automatic CO2 control
• Thermal Conductivity (T/C) or Infrared (IR)
– Available in-chamber HEPA filtration

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Easy to Use

ƒ Recessed power switch located on front of unit

ƒ CO2 sample ports conveniently located on front of unit

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 Available Copper Components

Solid Copper Chambers

ƒ 100% Coved Corners
Solid Copper Accessories
ƒ Duct Sheets
ƒ Shelf Supports
ƒ Shelves
ƒ Humidity Pans

Buy all Copper Components, or Mix and Match!

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 Choice of CO2 Controls

Automatic CO2
• TC-Thermal Conductivity
OR
• IR-Infrared

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User Conveniences

Front Access to Electronics in Slide-Out Drawer

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Available HEPA Filtration

HEPA

Optional system:
• In-chamber HEPA for easy replacement
• Filters 100% of chamber air within 60 seconds

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Dozens of Options

Factory Installed
– HEPA air filtration system
– Built-In Gas Guards (CO2 and N2)
– RH Readout with Alarm
– Full range of outputs
Customer Installed
– Inner glass doors
– Copper Shelf Systems
– Independent Inner Door Kit
– Roller Base
– Floor Stand

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Second-Generation Steri-Cult

The Best Incubator Ever Built

ƒ Thermo’s premium incubator
ƒ Still! largest available chamber in a
stackable CO2 incubator
ƒ Class-leading ease of maintenance &
upkeep
ƒ Fully compliant with current incubator “story”

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Steri-Cult Incubators

Total Control - Temp., CO2 and RH

ƒ Two Sizes:
• 8.2 cu.ft. (232 liters)
• 11.4 cu.ft. (322 liters) (largest cell culture
incubator chamber on the market)
ƒ Ultrasonic nebulizer for controlled RH
ƒ Advanced IR CO2 Sensor
ƒ Direct Heat

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Steri-Cult Features

ƒ Contamination control
• Water source outside of chamber
• Class 100 HEPA Filtered airflow
• Laminar Airflow for reduced cross-
contamination
• Validatable 140C sterilization
cycle
ƒ Customer convenience
• Inventory management system
• “Full extension” shelves
• Front access for all maintenance
features

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“Reach-in” CO2 Incubator

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Model 3950/3951

Small-Scale control in a large

cell-culture environment
ƒ 29 cu. ft. (821 liters)
ƒ Temperature and CO2 Control
ƒ Horizontal laminar airflow
ƒ Elevated Humidity Control
ƒ All Stainless Steel Interior
ƒ Advanced Microprocessor Controls

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Features

ƒ Heated Glass Door

ƒ Easy 3-way humidity reservoir fill system,
automatic or manual
ƒ 3 Humidity ranges
ƒ Standard dual access ports
ƒ Standard duplex outlets
ƒ Casters and leveling feet

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Control Features

ƒ Message display
ƒ Electronic startup
ƒ Audible/visual under & over Temp & CO2
ƒ Water level alarm
ƒ Standard remote alarm contacts

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Dozens of Options

ƒ Inner door kit

ƒ Light-tight door cover
ƒ Door light package (diurnal)
ƒ Door lock
ƒ 4 Enviro-Scan outputs
ƒ Temp & RH Recorders
ƒ Built-in Gas Guard
ƒ Reinforced shelves
ƒ Shaker shelves
ƒ Cell Roller systems

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