10/17/2019 Beetroot as an indicator

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Beetroot as an indicator
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10/17/2019 Beetroot as an indicator

Sudeera Namarathne, Junior Business Developer at Sana Commerce

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Published on Apr 18, 2012

Published in: Education, Technology, Business

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Gabi Tache , specialist at AXA- as part time / teacher at Nenitescu High School at AXA
very interesting experiment in order to make pupils understand what pH means.
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10/17/2019 Beetroot as an indicator

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Yash Cholera
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Sudeera Namarathne , Junior Business Developer at Sana Commerce at ISM APAC (Pvt) Ltd
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Ahmed Alsaeed
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Beetroot as an indicator
1. 1. Asela Oshadi Chamali PulasthiDhammika Saminda Dunhinda Sudeera Nuwan
2. 2. What is a pH indicator and its PH range…. Preparation of the Beetroot indicator and identification of its pH range. Applications of acid-base
titrations. Discussion. Conclusion. References.
3. 3. A pH indicator (An acid-base indicator ) is either a weak acid or weak base that exhibits a color change as the concentration of hydrogen (H+) or hydroxide
(OH-) ions changes in an aqueous solution. Examples: Thymol Blue, Phenolphthalein, Methyl Orange, Bromocresol Green, Bromophenol Blue and etc; An
Indicator’s colour change occurs over a range of hydrogen ion concentrations. This range is termed the colour change interval. It is expressed as the Indicator’s pH
range. Reference: Anne Marie Helmenstine, Ph.D., (2011). Acid-Base Indicators. Available: http://chemistry.about.com.
4. 4. Beetroot Red, is a red glycosidic food dye obtained from its aglycone, obtained by hydrolyzing the glucose molecule, Betanin. The colour of Betanin
depends on pH. It is bright bluish-red, becoming blue-violet as the pH increases. Once the pH reaches alkaline levels Betanin degrades by hydrolysis, resulting in a
yellow-brown colour.Moira Sheehan, Carol Chapman. (2003). Make your own indicator.Available: http://www.pearsonschoolsandfecolleges.co.uk.
5. 5. Preparation of the Indicator:1. 300.0 g of chopped Beetroot was added to 50.00 cm3 of boiling water in a beaker and was boiled continuously for 45 minutes.2.
After allowing the beaker to cool for 15 minutes, boiled Beetroot were squashed and the liquid was filtered.3. The residues were squeezed once again and the liquid
was evaporated to get a highly concentrated portion of the indicator.
6. 6. Detection of the pH range: A series of solutions in the range pH 1.0 -13.0 was prepared by using 1.0 M NaOH and 1.0 M HCl acid. When the indicator was
added the to the prepared series of solutions the colour was changed from red to yellow in between pH 10.0 -12.0. In order to get the exact pH range, another
series of solutions was prepared by diluting the pH 10-12 solution range into 10.10, 10.20,……..,12.00 and 0.05 ml of the indicator was added to each solution. A
colour change was observed in the pH range 10.80-11.20, which is the working pH range of Beetroot.
7. 7. i) Strong Acid – Strong Base Titration Colour Change: Red Yellowii) Strong Acid – Weak Base Titration A colour change was not observed.
8. 8. iii) Weak acid – Strong Base Titrations Colour Change: Red Yellow With 0.1M NaOH . A colour change was not observed. With 0.001M NaOH
9. 9. A colour change was observed only in strong acid – strong base titration and weak acid-strong base titration (with 0.10 M NaOH), as the working pH range of
the indicator falls to the vertical portion of those reactions. But when the base is diluted, a colour change was not observed, as the pH range of the Beetroot
indicator doesn’t fall within the rapid pH change of the reaction. Although a colour change was observed in above two titrations, the most applicable titration is
weak acid-strong base titration, because the vertical portion of the reaction shifts upwards.
10. 10. Working pH range of BEETROOT is pH 10.80 - 11.20 Acidic Basic RED YELLOW(Above result was obtained on 28th of March 2012 at 11.50 a.m. atB2
102 laboratory under the room temperature 270 C.) BEETROOT is a good indicator. But it is not a very suitable indicator for laboratory experiments as it should
be prepared at the time the experiment is done. The prepared indicator also cannot be kept for a long time as it is a biological sample.
11. 11. Anne Marie Helmenstine, Ph.D., (2011). Acid-Base Indicators. Available: http://chemistry.about.com. Last accessed 5th April 2012. Australian
Broadcasting Corporation(ABC). (2007). Teaching Science - Cool Chemistry(PH Indicator). Available: http://www.abc.net.au. Last accessed 4th April 2012.
Langes Handbook of Chemistry, 8th Edition, Handbook Publishers Inc; 1952. Moira Sheehan, Carol Chapman. (2003). Make your own indicator. Available:
http://www.pearsonschoolsandfecolleges.co.uk. Last accessed 2nd April 2012. Volumetric Analysis, Kolthoff & Stenge, Interscience Publishers, Inc; New York,
1942 and 1947.

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