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Cell Tonicity LAB
Cell Tonicity LAB
Osmosis is most easily defined as the diffusion of water, and diffusion defined is the movement of
substances from a high to low concentration. While this sounds simple, osmosis has a profound
impact on the processes of the human body. Within the human body, cells live within a fluid
environment that can contain a combination of dissolved particles, such as salt and potassium. Cells
have a semi-permeable membrane that separates the intracellular fluid (inside the cell) from the
extracellular fluid (outside the cell). Diffusion and osmosis between intracellular and extracellular
fluids depend on the number and types of dissolved particles inside and outside of the cell.
Scenario
A 58-year-old male patient has been admitted to HASPI Hospital with
severe dehydration due to constant vomiting and diarrhea for the
past 3 days. The first thing the hospital staff needs to do for this
patient is administer fluids intravenously, but the patient is demanding
he only be given “pure” water, and is refusing any other IV fluids.
You are responsible for patient education at HASPI Hospital. In order
to get the patient to agree to be administered the 0.9% saline IV fluids
he needs to rectify his dehydration, you must first educate him on
http://brucefong.files.wordpress.com/2010/04/sic
what will happen if he exposes his cells to “pure” water. k_man.gif?w=306&h=277
You have tried to explain cell tonicity, but the patient has demanded that you “show him, not tell him.”
The only items you have found in the break room kitchen that can help you demonstrate this concept
is a knife/scalpel, cups/beakers, raw potatoes, water, and salt. You also have weighing boats, a
balance scale, and a ruler you can use.
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Directions
Your job is to create an experiment using only the listed items to MEASURABLY demonstrate what
will happen when the potato cells are exposed to an isotonic, hypotonic, and hypertonic solution in
order to “show” the patient what will happen to his cells if he is given “pure” water by IV.
Experiment REQUIREMENTS
1. You must record the EXPERIMENT STEPS.
A. Experiment Steps:
List the steps of your experiment. Include exact amounts and measurements.
B. Data Table:
Create a data table that will summarize your results.
C. Measurable Results:
Explain how your experiment demonstrates each of the following solutions:
Isotonic Solution
Hypotonic Solution
Hypertonic Solution
D. Patient Explanation:
Explain how your results prove that pure water by IV could be harmful, and even possibly deadly.
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Analysis Questions - on a separate sheet of paper complete the following
1. Two solutions are separated by a semi-permeable membrane. Solution A has a 10%
concentration of sugar and solution B has a 40% concentration of sugar. What direction
would you expect the water to move, from A to B or from B to A? What is this process
called?
2. In the study of human physiology, why is it important to understand osmosis?
3. When the body needs to conserve water, the kidneys excrete a hypertonic urine. What do
the terms isotonic, hypotonic, and hypertonic mean? Draw beakers with red blood cells in
them to demonstrate isotonic, hypotonic, and hypertonic.
4. The receptors for thirst are located in a part of the brain called the hypothalamus. These
receptors, called osmoreceptors, are stimulated by an increase in blood osmolality. Imagine
a man who has just landed on a desert island. Trace the course of events leading to his
sensation of thirst. Can he satisfy his thirst by drinking seawater? Explain your answer.
5. Before the invention of refrigerators, pioneers preserved meat by salting it. Explain how
meat can be preserved by this procedure. (HINT: Think about what salting the meat would
do to decomposer organisms, like bacteria and fungi)
6. Research and describe two diseases/disorders related to electrolyte imbalance including
information about hypertonic and hypotonic conditions of nitrogen, calcium, sodium, and
potassium.
7. CONCLUSION: What is cell tonicity?
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