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Protease Opt Opt1
Protease Opt Opt1
Definition of a protease
• Protease = Peptidase = Proteinase = Proteolytic enzyme
Occurrence and
functionality
• Analysis of complete genomes has shown that about
2% of proteins in all kinds of organisms are proteases
» population increase
» improved economic prosperity
✓ Phase feeding
✓ Ideal protein ratios
✓ Low protein diets
✓ Synthetic amino acids
✓ Microbial (exogenous) protease enzymes
Proteases until now….
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Developing the ideal protease for feed
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RONOZYME® ProAct – a unique
protease specifically developed for
feed
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FOR INTERNAL USE ONLY
3x
7.5x
ELN-13-HALL-0016
FOR INTERNAL USE ONLY
Test enzyme
Analyse soluble
Feed protein
Analyse degree of
hydrolysis (DH)
The results reflect at the same time survival and action in the digestion model
Complements endogenous enzymes
Corn-sbm diet: pH 6/30 min -> pH 3/pepsin/10 min Wheat bran: pH 7/3 hours
Phytase @ recom. dose, ProAct @ 20x rec. dose Xylanase @ recom. dose, ProAct @ 10x rec. dose
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Effect of supplementing RONOZYME® ProAct to
low CP/AA wheat soya based broiler diets
Species: Broiler
Country: Belgium
Results
Performance at 39 days
Objective
• To determine the effect on broiler
2400 1.65
performance of supplementing RONOZYME®
Weight g
ProAct to wheat soy based diets with
FCR g/g
2350 1.6
reduced crude protein and amino acid
content from 11-39 days of age. 2300 1.55
2250 1.5
Trial details T1 T2 T3
• Institute for Agriculture and Fisheries Treatment
Research (ILVO), Belgium
• Breed: 710 male Ross 308 Weight FCR
• Completely randomized design, 7
replicate pens (control diets) and 8
replicate pens (experimental diets) each
of 32 birds
• Diets: Wheat, corn, soyabean meal, full
fat soy based. Non heat treated mash. Conclusions & Benefits
• Treatments*: • The NC diet produced reduced performance at day 39
T1= Positive control (PC) diet • RONOZYME®ProAct supplementation to the NC diet gave superior
performance when compared to the PC diet
T2 = Negative control (NC) diet - 3% less
• RONOZYME®ProAct is an effective addition to wheat soya based diets
Crude protein/Amino acid than T1 from that also contain xylanase and phytase enzymes
11-39 days
T3 = T2 + 200 ppm RONOZYME® ProAct
* All diets contained xylanase and phytase
Liveweight (kg)
reduced crude protein and amino acid 1.4
FCR (kg/kg)
3.7
8
content from 0-42 days of age. 2
3.6 1.4
8 7
3.6
1.4
Trial details 4
3.6 7
• Avian Science Research Centre, SAC, T1 T2 T3 1.4
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Scotland Liveweight 1.4
• Breed: 1116 male Ross 308 FCR 6
• Completely randomized design, 12 1.4
replicate pens of 31 birds per treatment 5
• Diets: Wheat, corn, soyabean meal, full
fat soy based. Pelleted.
• Treatments*:
T1= Positive control (PC) diet Conclusions & Benefits
T2 = Negative control (NC) diet - 3% less • The NC diet produced reduced performance at day 42
Crude protein/Amino acid • RONOZYME®ProAct supplementation to the NC diet gave superior
performance when compared to the PC diet
T3 = T2 + 200 ppm RONOZYME® ProAct • RONOZYME®ProAct is an effective addition to wheat soya based diets
* All diets contained xylanase and phytase that also contain xylanase and phytase enzymes
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Practical implementation into diet formulations
Less precise/implementation
quick