Professional Documents
Culture Documents
Course Structure MSC, Microbiology: Sem Component Sub Code Title of The Paper HRS/ Week Credit
Course Structure MSC, Microbiology: Sem Component Sub Code Title of The Paper HRS/ Week Credit
MSc, Microbiology
Total 30 17
Total 30 32
GENERAL MICROBIOLOGY
UNIT : I
Evolution of microbiology- contributions of Van Leeuwenhoek, Joseph Lister, Louis Pasteur
, Robert Koch, Edward Jenner, Winogradsky and Beijernick- Further developments in
microbiology- identification, characterization and classification of microorganisms- Distinguishing
characteristics between prokaryotic and eukaryotic cells- Phenotypic characters- Taxonomic
characters- Distinctive characters of major groups of microorganisms- Principles of classification-
Numerical taxonomy and taxonomic structuring- Genetic relationship- DNA homology- 16S rRNA
Sequencing.
UNIT : II
Microscopy- It’s principles and applications in the field of microbiology including the
following, Dark field, phase contrast, fluorescence microscopy, transmission and scanning electron
microscopy, confocal microscopy- colorimeter, spectrophotometer and lyophilizers- Staining
methods- Gram’s acid- fast, met-achromatic granules, nuclear, capsule, flagella, silver
impregnation and other special staining methods.
UNIT : III
Methods of sterilization: Physical and chemical agents, radiation and filtration- Indicator
microorganisms for sterilization methods- Cultivation of microorganisms- Microbiological media,
enrichment media, enriched media, transport media, selective media and pure culture technique-
Methods of preservation and maintenance of cultures- Role of disinfectants.
UNIT : IV
Bacterial anatomy, structure, properties and bio-synthesis of cellular components of
bacteria- Sporulation and it’s mechanism- Growth and nutrition- Nutritional requirements-
Autotrophs- Heterotrophs- Enrichment cultures- Growth curve- Kinetics of growth- Batch culture-
Synchronous growth- Measurement of growth and enumeration of cells- Techniques of pure
culture.
UNIT : V
Laboratory uses of animals with special reference to microbiology pathogenicity testing,
antibody production, and toxin/ toxoid testing, hypersensitivity testing, and maintenance of
microbes in animals- Care breeding and maintenance of laboratory animals: Rabbits, rats, mice,
guinea pigs, monkeys etc, Disposal of animal house wastes.
MICROBIAL DIVERSITY AND CLASSIFICATION
UNIT : I
UNIT : II
Fungi: Morphology, taxonomy and classification- detection and recovery fungi from clinical
specimens- Dermatophytes and agents of superficial mycoses- Yeast of medical importance –
Mycotoxins- Anti-fungal agents- Testing methods and quality control. Dimorphic fungi causing
systematic Mycoses- Dermataceous fungi, opportunistic hyaline hypomycetes, agents of
Zygomycoses- Fungi causing eumycotic mycetoma- Immunity to fungal infections.
UNIT : III
UNIT : IV
Algae: Distribution, general characters, thallus and its structure, classification, nutrition and
reproduction- Characters of selected groups- Blue green algae, Dinofalgellates, Euglenophyta,
Chrysophyta, Phaeophyta and Rhodophyta- Economic importance of alga biotechnology.
UNIT : V
UNIT : I
UNIT : II
UNIT : III
UNIT : IV
UNIT : V
UNIT : I
UNIT : II
UNIT : III
General principle and applications of chromatography- Paper, thin layer, column, gel
filtration, affinity and ion exchange chromatography- Advanced techniques (GC/ HPLC).
UNIT : IV
Electrophoresis- Horizontal and vertical- Moving boundary, zone (paper and gel)
electrophoresis- Immunoelectrophoresis- Rocket electrophoresis- Isoelectric focusing- Application
of each.
UNIT : V