Analytical Test Procedure

MEGHNA BEVERAGE LIMITED.
TOPORDI, SONARGOAN,nARAYANGONJ
ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 1
MATERIAL NAME: CITRIC ACID ANHYDROUS

Molecular Formula: C6H8O7 Formula Weight: 192.13 Equivalent Weight: 64.03
SL Test Parameters Specification

NO.
1. Physical State Colorless crystal or White Crystalline Powder
A neutral solution of citric acid is boiled with HgSO4 TS (5 g in 80 ml
2. Identification of water), then filtered. Few drops of KMnO4 TS (10 g/l) is added to
the filtrate, a white precipitate is formed.
Clarity 10 % W/W Solution Should Be Clear Compared With Existing Good
3.
One
4. Loss on drying NMT 1.0% w/w at 105°C for 15 minutes, use 2 g
5. Assay (%) 99.5 % to 100.5 % as C6H8O7 calculated on anhydrous basis.
METHOD OF ASSAY ANALYSIS

1. Place About 0.55 g Of Anhydrous Citric Acid In A Tarred Conical Flask &Weigh Accurately.
2. Dissolve In 50ml Of Water.
3. Add 0.5ml Of Phenolphthalein TS.
4. Titrate With 1N Sodium Hydroxide VS.
5. End Point Is Determined When Just Pink Color Appeared.
CALCULATION OF ASSAY PERCENTAGE

Each ml Of 1N Sodium Hydroxide Is Equivalent To 64.03 mg Of C 6H8O7
Or
BR × Normality Of NaOH ×Equivalent Weight Of Citric Acid(64.03)×100
Sample Weight×1000
Precautions:
1. Standardize Sodium Hydroxide Solution Before Titration.
2. Weighing Should Be Accurate
REFERENCE: USP-29
Name Designation Sign & Date

Prepared By
Reviewed By S.M. Ayenul Islam Manager, QA
Authorized By
MATERIAL NAME: TRI SODIUM CITRATE DIHYDRATE

Molecular Formula: C6H5Na3O7.2H2O Formula Weight: 294.10 Equivalent Weight: 86.02

NO.
1. Physical State Colorless crystal or White Crystalline Powder
A solution of sodium citrate is boiled with HgSO4 TS (5 g in 80 ml of
2. Identification water), then filtered. Few drops of KMnO4 TS (10 g/l) is added to the
filtrate, a white precipitate is formed.
Clarity 10 % W/W Solution Should Be Clear Compared With Existing Good
3.
One

1. Place About 0.35 g Of sample previously dried at 180°c for 18 hours & Weighed Accurately to a
250 ml conical flask.
2. Add 100 ml of glacial acetic acid & dissolve properly.
3. Titrate With 1.0 N Perchloric acid VS using crystal violet TS, until bluish green color appeared
4.Perform a blank titration

Each ml Of 0.1N perchloric acid is Equivalent To 8.602 mg Of C6H5Na3O7
i.e
BR × Normality Of perchloric acid ×Eq.wt. Of sodium citrate(86.02)×100
Sample Weight×1000
Precautions:
1. Standardize per chloric acid Solution before Titration.
REFERENCE: USP-29

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MATERIAL NAME: SODIUM BENZOATE

Molecular Formula: C7H5NaO2 Formula Weight: 144.10 Equivalent Weight: 144.1

NO.
1. Physical State White Crystalline or granular Powder
1. When a solution of benzoate (1g in 20 ml of water) is
acidified with dilute HCl, crystalline ppt is formed.
2. Identification 2. When FeCl3 solution is added to a neutral solution of
benzoate (1g in 20 ml of water), reddish brown ppt is
formed.
Clarity 5 % W/W Solution Should Be Clear Compared With
3.
Existing Good One
4. Loss on drying NMT 2.0% w/w at 105°C for 15 minutes, use 1 g

1. Place About 0.6 g Of Accurately Weighed sample to a 250 ml conical flask.
2. Add 100 ml of glacial acetic acid & dissolve properly.
3. Add 2 drops of crystal violate TS.
3. Titrate With 0.1 N Perchloric acid VS untill green color appeared.
4. Perform a blank titration .

Each ml Of 0.1 N perchloric acid Is Equivalent To 14.41 mg Of C7H5NaO2
Precautions:
1. Standardize Perchloric acid Solution Before Titration.
REFERENCE: USP-29
MATERIAL NAME: COLA KIT PART-02 (PHOSPHORIC ACID)

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Molecular Formula: H3PO4 Formula Weight: 98 Equivalent Weight: 49

NO.
1. Physical State Liquid could crystalize
2. Color Colorless
Identification When silver nitrate solution is added to a neutral solution of H3PO4,
3.
yellow color precipitate is formed.
4. Specific gravity(d20/20) 1.686 to 1.730
5. Refractive index(nd/20) 1.431 to 1.434
6. Acid content 84.0 % to 86.0 % as H3PO4

1. Weigh accurately about 1 g Of phosphoric acid in a tared glass stoppered flask & dilute it with 120
ml of water.
2. Add 0.5 ml of Phenolphthalein TS.
3. Titrate with 1 N NaOH VS Until blue color is appeared.
4. Perform a blank titration.

Each ml Of 1N NaOH is Equivalent To 49 mg Of H3PO4
i.e
BR × Normality Of NaOH ×Eq.wt. Of Phosphoric acid (49)×100
Sample Weight×1000
Precautions:
1. Standardize NaOH Solution Before Titration.
REFERENCE: USP-29

Prepared By
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MATERIAL NAME: FERROUS SULFATE HEPTAHYDRATE

Molecular Formula:FeSO4.7H20 Formula Weight: 278.02 Equivalent Weight: 278.2 g

NO.
1. Physical State Light green Crystalline powder
When NaOH solution is added to ferrous salt solution, gel like white
Identification ppt is formed. If this is well shaken, the color becomes greyish &
2.
gradually turns reddish brown. When sodium sulfide solution is
added, the color of ppt changes to black.
3. pH 3.0 to 4.0 of 5% solution
4. Assay (%) 99.5% to 104.5 % as FeSO4.7H20

1. Take about 0.5 g of accurately weighed sample.
2. Transfer it in a mixture of 25 ml of diluted sulfuric acid & 25 ml of freshly boiled & cooled water.
3. Titrate with 0.1 N KMnO4

Each ml Of 0.1N KMnO4 Is Equivalent To 27.8 mg Of FeSO4.7H20
i.e
BR × Normality Of KMnO4 ×27.8
Sample Weight
Precautions:
1. Standardize KMnO4 Solution Before Titration.
REFERENCE: USP29

Prepared By
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MATERIAL NAME: CALCIUM HYPOCHLORITE (BLEACHING POWDER)

Molecular Formula: Ca(Ocl)Cl Formula Weight of chlorine: Equivalent Weight: 35.46 g
35.46

NO.
1. Physical State White granular powder
When 2 ml of acetic acid to 0.1 g of bleaching powder, gas is
Identification evolved & it dissolves. 5 ml of water is added then filtered.
2.
Ammonium oxalate solution ( 1 g in 30 ml of water) is added to the
filtrate with HCl, white ppt is formed.
3. Purity (%) 65% to 70 % as available chlorine

1. 5 gm of sample is weighed accurately & mixed with 500 ml of water using a morter.
2. Transfer 25 ml 0f stock solution into a conical flask.
3. Add 2g KI+10ml glacial acetic acid.
4. Titrate with 0.1N Na2S2O3 using starch indicator until colorless solution is appeared.

Each ml of 0.1 N Na2S2O3 is equivalent to 3.546 mg of Cl2
Calculation(% available Cl2): BR×0.003546gm×100/0.25
Precautions:
1. Standardize Na2S2O3 Solution Before Titration.
REFERENCE: USP29

Prepared By
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MATERIAL NAME: CALCIUM OXIDE (LIME)

Molecular Formula: CaO Formula weight: 56.08 Equivalent Weight: 28.04

NO.
1. Physical State White hard lump
20 ml of water is added to 1 g of CaO. Acetic acid is added until it
2. Identification dissolves, then filtered. Ammonium oxalate solution ( 1 g in 30 ml of
water) is added to the filtrate with HCl, white ppt is formed.
3. Assay (%) Minimum 66% as CaO calculated as anhydrous basis.

1. Ignite about 1 g of sample to constant weight, cool, weigh accurately.
2. Dissolve in 20 ml of 3N HCl. Cool the solution.
3. Transfer to a 500 ml volumetric flask & up to the mark with water.
4. Take 50 ml of stock solution & add 100 ml of water,15 ml of 1N NaOH & 300 mg of Hydroxy
naphthol blue.
5.Titrate with 0.05M EDTA VS until the solution is deep blue in color

Each ml of 0.05M EDTA is equivalent to 2.804 mg of CaO
Precautions:
1. Standardize EDTA Solution Before Titration.
REFERENCE: USP-29

Prepared By
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MATERIAL NAME: SODIUM CHLORIDE

Molecular Formula: NaCl Formula weight: 58.44 Equivalent Weight: 58.44

NO.
1. Physical State White crystalline powder
It responds the test for sodium & chloride.
Chloride: Dissolve about 3 mg of NaCl in 2 ml of water. Acidify
2. Identification with dilute nitric acid & add 0.4 ml of AgNO3 TS. Shake & allow to
stand. A curdled, white ppt is formed, which is dissolve by adding
excess amount of ammonia solution.
3. Solubility Soluble in water but insoluble in absolute ethanol.
4. Assay (%) 99.0 % to 100.5%(refined) & 96% minimum for commercial grade

1. Dissolve 0.05 g of sample & dilute with 100 ml of water.
2. Add 2 or 3 drops of potassium chromate TS.
2. Titrate with 0.1 N Silver Nitrate VS, until brick red color is appeared.

Each ml of 0.1 N silver nitrate is equivalent to 5.844 mg of NaCl
Or
BR × Normality Of silver nitrate ×5.844
Sample Weight
Precautions:
1. Standardize silver nitrate Solution Before Titration.
REFERENCE: USP-29
MATERIAL NAME: ASCORBIC ACID (VITAMIN-C)

Prepared By
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Molecular Formula: C6H8O6 Formula weight: 176.12 Equivalent Weight: 88.06

NO.
1. Physical State Colorless or white crystalline powder
When 1 ml of potassium permanganate solution is added to 10 ml
2. Identification of an aqueous solution of vitamin-c ( 1 g in 100 ml water), the color
disappears immediately.
3. pH 2.1 to 2.6 of 5% w/v solution
4. Solubility Soluble in water & ethanol but insoluble in Chloroform & ether
5. Assay (%) 99% to 100.5 % as C6H8O6 calculated as anhydrous basis.

1. Dissolve about 0.4 g accurately weighed sample.
2. Add 100 ml water & 25 ml of 2N Sulfuric acid.
3. Add 3 ml starch solution TS.
4. Titrate at once with 0.1 N Iodine solution VS,until violet blue color is obtained.

Each ml of 0.1 N Iodine is equivalent to 8.806 mg of C6H8O6
or
BR × Normality Of iodine solution ×8.806
Sample Weight
Precautions:
1. Titrate with iodine instantly.
2. Standardize iodine solution before titration.
3. Weighing should be accurate.
4. Handle iodine solution carefully.
REFERENCE: USP-29
MATERIAL NAME: ASPARTAME

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Molecular Formula: C14H18N2O5 Formula weight: 294.30 Equivalent Weight: 294.30

NO.
Dissolve 20 mg of aspartame in 1 ml of methanol, add 0.5 ml of
methanol saturated with hydroxylamine hydrochloride. Mix, then
2. Identification add 0.3 ml of 35 % methanolic KOH solution & boil. cool then adjust
the pH with 0.1N HCl. Then add FeCl3 solution (1 g in 100 ml of
water). A deep red purple color develops.
3. Taste Has a strong sweet taste
4. pH 4.5 to 6.0 (0.8 % solution)
5. Assay (%) 98 to 102.0 % of C14H18N2O5 Calculated on anhydrous basis.

1. Accurately weighed 0.3 g of sample.
2. Dissolve in 1.5 ml of formic acid.
3. Add 60 ml of glacial acetic acid then add crystal violet TS.
4. Immediately titrate with 0.1 N perchloric acid VS to a green end point.
5. Perform a blank determination.

Each ml of 0.1 N Perchloric acid is equivalent to 29.43 mg of C 14H18N2O5
Or
BR(blank-sample) × Normality Of Perchloric acid ×29.43
Sample Weight
Precautions:
2. Weighing Should Be Accurate.
REFERENCE: USP-29
MATERIAL NAME: ACESULFAME-K

Prepared By
Authorized By

Molecular Formula: C4H4NO4SK Formula weight: 201.24 Equivalent Weight: 201.24

NO.
0.2 g of sample is dissolved in 2 ml of acetic acid (30 ml in 100 ml of
2. Identification water), & 2 ml of water. Upon adding a few drops of sodium cobalt
nitrite solution, yellow ppt are formed.
3. Taste Has a strong sweet taste
4. Moisture 0.2 maximum
5. pH 5.5 to 7.5 (1% solution)
6. Assay (%) 99.0 to 101.0 % of C4H4NO4SK

1. Dissolve about 0.15 g of sample in 50 ml of glacial acetic acid.
2. Add crystal violet TS.
3. Immediately titrate with 0.1 N perchloric acid VS to a bluish green end point.
4. Perform a blank determination.

Each ml of 0.1 N Perchloric acid is equivalent to 20.12 mg of C4H4NO4SK
Or
BR( blank – sample) × Normality Of Perchloric acid ×20.12
Sample Weight
Precautions:
REFERENCE: USP-29

Prepared By
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MATERIAL NAME: RBD PALM OLEIN

NO.
1. Physical State Liquid
Color Pale yellow
2. Peroxide value Maximum 1 meq/kg
3. FFA value Maximum 0.2 %
4. Odor No off odor found
METHOD OF PEROXIDE VALUE ANALYSIS

1.Take 5gm sample then add 30ml acetic acid: chloroform solution(480 ml acetic acid & 320 ml
chloroform)
2. Add 0.5ml KI solution, stopper the flask & swirl exactly 1 min.
3. Add 30 ml of water. Shake vigorously to liberate iodine from the chloroform layer. Deep orange
color appeared.
4. Titrate with 0.1 N Na2S2O3 VS.
5. If initial color is deep orange, titrate slowly until the color lighten using starch indicator (1%). If
initial color is light amber, titrate until blue gray color disappear in upper layer.
6. Conduct a blank titration of the reagent.
CALCULATION OF PEROXIDE VALUE
Calculation: BR (Titration of sample – Titration of blank)× Normality Of Na2S2O3 ×1000

Sample Weight
Precautions:
1. Standardize Na2S2O3 Solution Before Titration.
REFERENCE-AOCS (AMERICAN OIL CHEMIST’S SOCIETY)

Prepared By
Authorized By
MATERIAL NAME: RBD PALM OLEIN

NO.
1. Physical State Liquid
Color Pale yellow
2. Peroxide value Maximum 1 meq/kg
3. FFA value Maximum 0.2 %
4. Odor No off odor found
METHOD OF FREE FATTY ACID ANALYSIS
1. Take 10 g of oil sample
2. Add 10 g of methanol to dissolve the sample.
3. Add 2 to 3 drops of phenolphthalein TS.
4. Titrate with 0.025 N NaOH VS.
5. Conduct a blank titration.
CALCULATION OF FFA PERCENTAGE

% FFA= BR (Titration of sample – Titration of blank)× Normality Of NaOH ×28.2
Sample Weight
OR
% FFA= 0.766× T (Titration result in ml) for NaOH
NOTE: A. 28.2 is the equivalent weight of oleic acid. That is 282/10= 28.2
B. 1 ml of oil= 0.92 g
Precautions:
1. Blank titration must be done.
2. NaOH Should be standardized.
REFERENCE: PORAM (THE PALM OIL REFINERS ASSOCIATION OF MALAYSIA)

Prepared By
Authorized By
MATERIAL NAME: NITRIC ACID (COMMERCIAL GRADE)

Molecular Formula: HNO3 Formula weight: 63.01 Equivalent Weight: 63.01

NO.
1. Physical State Clear, Colorless fuming liquid
When ferrous sulfate solution is added on the top of a cooled
2. Identification mixture (1:1) of nitrate solution & sulfuric acid, a dark brown band
is formed at the interface.
3. Specific gravity 1.41
4. Purity (%) 68.0 to 71.0 %

1. Weigh accurately 2 ml of HNO3 in a conical flask.
2. Add 25 ml of water.
3. Add methyl red TS.
4. Titrate with 1 N NaOH VS.

Each ml of 1 N NaOH is equivalent to 63.01 mg of HNO3
Or
BR × Normality Of NaOH solution ×6.301
Sample Weight
Precautions:
REFERENCE: USP-29
MATERIAL NAME: SODIUM HYDROXIDE

Prepared By
Authorized By

Molecular Formula: NaOH Formula weight: 40.0 Equivalent Weight: 40.0

NO.
1. Physical State White crystalline pallets, flakes
It respond the test for sodium.
2.
Sodium: Acidify the solution with acetic acid (60 g/l). filter if
Identification necessary & treat with zinc acetate TS. A yellow crystalline ppt is
formed.
3. Purity (%) 97.0 to 100.5 % of total alkali

1. Dissolve 1.5 g of sample in 40 ml of carbon dioxide free water.
2. Cool the solution to room temperature.
3. Add phenolphthalein TS.
4. Titrate with 1 N H2SO4 VS.
5. At The discharge of pink color , record the volume of acid required.
6. Add methyl orange TS & Continue titration until a persistent pink color is produced.
BR(Using Phenolphthalein TS) × Normality Of H2SO4 solution ×4

Sample Weight
&
BR(Using Methyl Orange TS) × Normality Of H2SO4 solution ×10.6
Sample Weight
Precautions:
1. Standardize H2SO4 Solution Before Titration.
REFERENCE: USP-29

Prepared By
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MATERIAL NAME: SODIUM CARBONATE

Molecular Formula: Na2CO3 Formula weight: 105.99 Equivalent Weight: 52.99

NO.
1. Physical State A white, slightly granular powder
2. Loss on drying Not more than 1.0 %, use 1 g.
3. Assay (%) 99.5 % to 100.5 % of Na2CO3 as dried basis.

1. Dissolve 1 g sample in 25 ml of water.
2. Add 0.2 ml of methyl orange TS.
3. Titrate with 1M HCl VS until color changes from yellow to red.

Each ml of 1 N HCl is equivalent to 52.99 mg of Na2CO3
Or
BR × Normality Of HCl solution ×5.299
Sample Weight
Precautions:
1. Standardize HCl Solution Before Titration.
REFERENCE: BP 1993

Prepared By
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MATERIAL NAME: HYDROCHLORIC ACID

Molecular Formula: HCl Formula weight: 36.46 Equivalent Weight: 36.46

NO.
1. Physical State A Clear, colorless, fuming liquid.
It responds the test for chloride.
Chloride: Dissolve about 3 mg of sample in 2 ml of water. Acidify
2. Identification with dilute nitric acid & add 0.4 ml of AgNO3 TS. Shake & allow to
stand. A curdled, white ppt is formed, which is dissolve by adding
excess amount of ammonia solution.
4. Purity (%) 35 to 39 % w/w. minimum 30% for commercial grade.

1. Weight 1.5 ml sample into a glass stoppered flask containing 30 ml of water.
3. Titrate with 1M NaOH VS.

Each ml of 1 N NaOH is equivalent to 36.46 mg of HCl.
Or
BR × Normality Of NaOH solution ×3.64
Sample Weight
Precautions:
REFERENCE: BP 1993
MATERIAL NAME: SODIUM BICARBONATE (BAKING POWDER)

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Molecular Formula: NaHCO3 Formula weight: 84.01 Equivalent Weight: 84.01

NO.
1. Physical State White crystalline powder or crystalline lumps
When magnesium sulfate solution is added to bicarbonate solution
2. Identification (1 g in 100 ml of water), white ppt is formed. When dil. Acetic acid is
added, the ppt dissolves.
Clarity When 1 g of sample is dissolve in 20 ml of water, the solution
3.
should be clear.
4. Assay (%) 99 % to 100.5% calculated as anhydrous basis.

1. Weigh about 3 g of sample, mix with 100 ml of water.
3. Titrate with 1 N HCl VS.
4. Add the acid slowly with constant stirring until the solution become faintly pink.
5. Heat the solution to boil, then cool.
6. Continue the titration until the faint pink color no longer fades after boiling.

Each ml of 1 N HCl is equivalent to 84.01 mg of NaHCO3
Or
BR × Normality Of HCl solution × 8.401
Sample Weight
Precautions:
REFERENCE: USP-29

Prepared By
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MATERIAL NAME: SULFURIC ACID

Molecular Formula: H2SO4 Formula weight: 98.08 Equivalent Weight: 49.04

NO.
1. Physical State A Clear, colorless, fuming liquid.
Identification When BaCl2 solution is added to sulfate solution, white ppt is
2.
formed.
4. Purity (%) 97 % to 98 % w/w

1. Place about 1 ml of accurately weighed sample in a glass stoppered flask containing 20 ml of
water.
2. Again weigh to obtain the weight of test specimen.
3. Dilute to about 25 ml of water, then cool.
4. Add methyl orange TS.
5. Titrate with 1N NaOH VS.

Each ml of 1 N NaOH is equivalent to 49.04 mg of H2SO4
Or
BR × Normality of NaOH solution ×4.904
Sample Weight
Precautions:
REFERENCE: USP-29

Prepared By
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MATERIAL NAME: HYDROGEN PEROXIDE

Molecular Formula: H2O2 Formula weight: 34.01 Equivalent Weight: 17.005

NO.
1. Physical State A Clear colorless liquid
To a 1:1 mixture of ethyl acetate & peroxide solution, 1-2 drops of
potassium bicromate solution is added. When the solution is
2. Identification acidified with dil. H2SO4 , the aqueous layer becomes blue.when the
mixture is shaken & settled to separate, the blue color migrates to
ethyl acetate layer.
4. Purity (%) 30 % contain 29 % to 32% w/w of H2O2

1. Accurately weighed about 1 ml of sample & dilute it to 100 ml with water.
2. Take 20 ml of stock solution & add20 ml of 2 N sulfuric acid.
3. Titrate with 0.1 N KMnO4 VS.

Each ml of 0.1 N KmnO4 is equivalent to 1.701 mg of H2O2
Or
BR × Normality Of KmnO4 solution ×1.701
Sample Weight
Precautions:
1. Standardize KmnO4 Solution Before Titration.
REFERENCE: BP 1993
ACTIVATED CARBON

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NO.
Physical State Black fine powder for sugar plant
1. &
Black granular powder for WTP
2. Moisture ≤10 % for sugar plant & ≤5.0 % for WTP
3. pH 2% to 5% for sugar plant (1 % solution)
4. Iodine value Minimum 850 mg/g
NOTE: Specification may change based on COA & Supplier.
METHOD OF IODINE VALUE ANALYSIS

Blank Titration
1. Take 10 ml of 0.1 N Iodine solution.
2. Add 2 drops of 1% starch solution.
3. The pale yellow color of iodine solution turns blue.
4. Titrate with 0.05 N sodium thiosulfate till it becomes colorless.
5. This burette reading corresponds to (B).
Sample titration
1. Weigh very accurately 0.2 g of activated carbon.
2. Introduce it into completely dry iodine flask.
3. Add 40 ml of 0.1 N Iodine solution.
4. Shake the flask for 4 minutes and filter it.
5. Collected the filtrate in a dry flask.
6. Titrate 10 ml of the filtrate against 0.05 N thiosulfate solution.
7. Burette reading corresponds to (A).

Iodine value= C (B-A)×Conversion factor
Here, Factor= Molecular weight of iodine (127) × Normality of iodine×40/ Weight of carbon × blank
reading.
Note: Iodine value is expressed in mg/g of carbon
REFERENCE: The complete guide of water chemistry & treatment. Writer: T.P.
Sivanandan

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MATERIAL NAME: STANDARD SUPERCEL FILTER AID

NO.
1. Physical State Creamy powder
2. Moisture 0.7 % at 105°C for 15 minutes
3. Bulk density 0.34 g/ml
MATERIAL NAME: HYFLO SUPERCEL FILTER AID

NO.
1. Physical State Creamy powder
2. Moisture 0.4 % at 105°C for 15 minutes
3. Bulk density 0.35 g/ml
4. pH <10
REFERENCE: COA BASED

Prepared By
Authorized By
REFINED SUGAR

NO.
1. Physical State White crystal
2. Moisture 0.05 % for Igloo & 0.04 % for Teer sugar
3. Purity 99.85 % minimum for Igloo & 99.82 % for Teer sugar
4. Conductivity ash % <0.016 % for Igloo & <0.0051 % for Teer sugar
5. Color ICUMSA <70 (IU) for Igloo & 38(IU) for Teer sugar
6. Clarity 50% w/w sugar solution should be clear.
METHOD OF PURITY TEST

Take 15 g sugar & 85 g water. It will be 15° brix solution.
Purity %= Obtained brix × 100/15
METHOD OF ASH TEST

Take 28 g sugar & 72 g water. It will be 28° brix solution.
Purity %= Conductivity of sugar solution – Conductivity of distilled water/1666.7
REFERENCE: AMSRL & CSIL
P3-TOPAX 56 ( ACIDIC CLEANING AGENT FOR FOAM CLEANING)

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NO.
1. Appearance Clear colorless liquid
Identification When silver nitrate solution is added to a neutral solution of H3PO4,
2.
yellow color precipitate is formed.
3. Specific gravity 1.160 – 1.200
4. Viscosity 4.0 to 6.0 mPas (20°C)
5. Specific conductivity 5.8 mS/cm ( 1%, 20°C)
6. pH 1.9 to 2.1 (1%, 20°C)
METHOD OF CONCENTRATION ANALYSIS AS PHOSPHORIC ACID

1. Take 50 ml of sample.
3. Titrate with 1.0 N NaOH VS.
CALCULATION OF CONCENTRATION PERCENTAGE

BR ×0.26= % ( by weight) Active as H3PO4
0.26= Titration factor
RECMMENDED USE % = 2 to 5 % with contact time 10 to 15 minutes.
REFERENCE: ECOLAB

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P3-TRIQUART (NEUTRAL DISINFACTANT)

NO.
1. Appearance Clear colorless to light yellow liquid
2. Specific gravity 0.980 to 1.000
3. Refractive index 1.3960 to 1.4020
4. °Brix 38.0 to 41.0
5. pH 7.00 to 8.50 (100% solution)
RECMMENDED USE % = 0.1 to 0.3 % with contact time 1 to 4 hours.
P3-LUBODRIVE (LIQUID SOAP FREE LUBRICANT FOR CHAIN CONVEYORS)

Molecular Formula: Formula weight: Equivalent Weight:

NO.
1. Appearance Clear yellowish liquid
3. pH 6.40 to 6.80 (100% solution)
RECOMMENDED USE % =0.2 to 0.4 %
REFERENCE: ECOLAB

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P3-TOPAX 19 (ALKALINE CLEANING AGENT FOR FOAM CLEANING)

NO.
1. Appearance Clear yellowish liquid
It respond the test for sodium.
Identification Sodium: Acidify the solution with acetic acid (60 g/l). filter if
2.
necessary & treat with zinc acetate TS. A yellow crystalline ppt is
formed.
3. Density at 20°C 1.21 to 1.23 g/cm3
4. pH 12.4 to 12.6 (1 % solution at 20°C)
5. Conductivity 9.08 mS/cm (1 % solution at 25°C)
TA 3.7 to 4.3 ml of 0.5 N HCl is needed to find the end point of 50 ml of
6.
1% solution using phenolphthalein TS.
7. % Active 11.40 to 11.70 as NaOH
METHOD OF CALCULATING OF % ACTIVITY

1. Take 50 ml of sample.
3. Titrate with 0.5 N HCl VS.
CALCULATION OF ACTIVITY PERCENTAGE

BR ×0.25= % ( by weight) Active as NaOH
0.25= Titration factor
RECMMENDED USE % = 2 to 5 % with contact time 10 to 15 minutes.
Precautions:
2. Sample must be 50 ml.

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REFERENCE: ECOLAB
DRY TECH-1 (SILICONE BASED CONVEYOR LUBRICANT) FOR TETRAPAK

NO.
1. Appearance White milky liquid
2. Specific gravity at 20°C 1.01
3. pH 3.5 TO 4.0
DRY TECH-7(SILICONE FREE CONVEYOR LUBRICANT) FOR PET BEVERAGE

NO.
1. Appearance Pale yellow clear liquid
2. Specific gravity at 25°C 1.03-1.07
3. pH 4.0 -7.5
SU 321 (DETERGENT DISINFECTANT)

NO.
1. Appearance Clear, Colorless to Pale yellow liquid
2. Specific gravity at 25°C 1.0 to 1.1
3. pH 11.0 to 12.0 ( 1% solution)
REFERENCE: JohnsonDiversey

Prepared By
Authorized By
P3-OXONIA ACTIVE 150 (ACID DISINFECTANT)

NO.
1. Appearance Clear colorless liquid with no particle
3. % H2O2 23.5 to 25.0
4. Density 1.13 g/cc
5. pH 2.8 (1%, 20°C)
METHOD OF H2O2 & PERACETIC ACID ANALYSIS

1. Take 1ml of sample & dilute it to 100 ml with water, then add 20 ml of 25% sulfuric acid.
2. Titrate with 0.1 N KMnO4 Solution until permanent pink color appeared.
3. Then add 5 ml of 10% KI Solution & 2 ml of 1% starch indicator & titrate with 0.1 N sodium thiosulfate until
colorless.
CALCULATION OF H2O2 PERCENTAGE

Each ml of 0.1 N KMnO4 is equivalent to 1.701 mg of H2O2.
BR of KMnO4 × Normality Of KMnO4 solution ×1.701
Sample Weight
CALCULATION OF PERACETIC ACID PERCENTAGE

Each ml of 0.1 N sodium thiosulfate is equivalent to 3.8 mg of PAA.
OR
BR for thiosulfate solution × Normality Of thiosulfate solution ×3.8
Sample Weight

Prepared By
Authorized By
REFERENCE: ECOLAB
DIVOSAN FORTE VT6 (ACID DISINFECTANT)

NO.
1. Appearance Clear colorless liquid
pH (1% solution at 2.5 – 4.5 (1% solution at 25°C)
3.
25°C)
METHOD OF H2O2 & PERACETIC ACID ANALYSIS

1. Take 1ml of sample & dilute it to 100 ml with water, then add 20 ml of 25% sulfuric acid.
2. Titrate with 0.1 N KMnO4 Solution until permanent pink color appeared.
3. Then add 5 ml of 10% KI Solution & 2 ml of 1% starch indicator & titrate with 0.1 N sodium thiosulfate until
colorless.
CALCULATION OF H2O2 PERCENTAGE

Each ml of 0.1 N KMnO4 is equivalent to 1.701 mg of H2O2.
BR of KMnO4 × Normality Of KMnO4 solution ×1.701
Sample Weight
CALCULATION OF PERACETIC ACID PERCENTAGE

Each ml of 0.1 N sodium thiosulfate is equivalent to 3.8 mg of PAA.
OR
BR for thiosulfate solution × Normality Of thiosulfate solution ×3.8

Prepared By
Authorized By

Sample Weight
Precautions:
1. Standardize KMnO4 & sodium thiosulfate & Solution before Titration.
DICOLUBE RS 148 NEU

NO.
1. Appearance Clear, slightly yellow liquid
3. pH (1% solution) 6.1
SU 727 ( OXOFOAM) CHLORINATED FOAM CLEANER

NO.
1. Appearance Clear pale yellow liquid
3. pH (1% solution) 12.7

Prepared By
Authorized By

P3-LUBOKLAR EC (SOAP FREE CHAIN LUBRICANT FOR CONVEYORS)

NO.
1. Appearance Clear yellow liquid
3. pH 6.40 to 6.80 (100% solution)
P3-STABICIP ABF (WETTING AGENT)

NO.
1. Appearance Clear colorless to slightly yellow liquid
3. Refractive index 1.371 to 1.383

Prepared By
Authorized By
REFERENCE: ECOLAB
P3-OXYPAK-D (LIQUID SANITIZER FOR PACKING MACHINE)

NO.
1. Appearance Clear, Colorless liquid
3. pH 2.1 to 2.6
4. Concentration 33 to 37 % as hydrogen peroxide
1. Accurately weighed about 1 ml of sample & dilute it to 100 ml with water.
2. Take 20 ml of stock solution & add20 ml of 2 N sulfuric acid.
3. Titrate with 0.1 N KMnO4 VS.

Each ml of 0.1 N KMnO4 is equivalent to 1.701 mg of H2O2
Or
BR × Normality Of KMnO4 solution ×1.701
Sample Weight
RECOMMENDED USE % = 100% & Contact time 4 to 8 seconds at 60 to 80°c.

Prepared By
Authorized By
REFERENCE: ECOLAB
FOOD GRADE FLAVOR IN POWDER FORM (CHEESE PUFFS)

NO.
1. Appearance Orange colored powder
2. Moisture 1.7 %
3. NaCl 11.7 % to 12.7 %
REFERENCE: PERESSCOL SDN.BHD
FOOD GRADE FLAVOR IN POWDER FORM (TANGY TOMATO)

NO.
1. Appearance Fine powder with uniform distribution of spices
2. Moisture 6.00 % (max.)
3. NaCl 2.30 % to 4.30 %
REFERENCE: PERESSCOL SDN.BHD
CLEAR LEMON KIT PART-1

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Authorized By

NO.
1. Appearance Oily & Limpid liquid
2. Color Pale yellow to pale green
3. Specific gravity(d20/20) 1.032 to 1.052 at 20°c
4. Refractive index(nD/20) 1.429 to 1.439 at 20°c
Solution comparison 0.1 % (Beverage conc.) solution should be identical in TOA as
5.
compared with existing good one.
REFERENCE: FIRMENICH (SWITZERLAND)
TUTTI FRUITY FLAVOURING

NO.
1. Appearance Oily & Limpid liquid
2. Color Pale yellow
5.
REFERENCE: FIRMENICH (SWITZERLAND)
COLA KIT PART-1

NO.
1. Appearance Liquid
2. Color Dark brown

Prepared By
Authorized By

5.
REFERENCE: FIRMENICH (BELGIUM)
MANGO FLAVOUR

NO.
1. Appearance Clear liquid
2. Color Colorless to light yellow
Solution comparison 0.103 % (juce conc.) solution should be identical in TOA as
5.
REFERENCE: SYMRISE
CLOUDY LEMON KIT PART-1

NO.
1. Appearance Viscous & cloudy liquid

Prepared By
Authorized By

2. Color Cream
5.
REFERENCE: FIRMENICH (BELGIUM)
ORANGE FLAVOR EMULSION E-204404

NO.
1. Appearance Emulsion
2. Color Whitish to pale yellow
4.
REFERENCE: GIVAUDAN (SINGAPORE)
CEREAL HERBAL BEVERAGE CONCETRATE ( COMP MALT)

Prepared By
Authorized By

NO.
1. Appearance Should be acceptable
2. °Brix 62.0 to 68.0
3. pH 3.30 to 4.30 at 20°c
REFERENCE: WILD (GERMANY)
MALT FLAVOUR

NO.
2. Color Pale yellow to pale green
4. Refractive index(nD/20) 1.4130 to 1.4530
5.

Prepared By
Authorized By
COMP GRAPE LEMON

NO.
2. Citric acid content 1.34 to 1.98 %
3. °Brix 53.7 to 55.7
GREEN MANGO FLAVOUR

NO.
1. Appearance Liquid
2. Color Colorless
REFERENCE: FIRMENICH (INDONESIA)

Prepared By
Authorized By
APPLE FLAVOURING

NO.
4.
ROUNDING FLAVOUR

NO.
4.

Prepared By
Authorized By
VITAMIN PREMIX HA3 F072000013

NO.
1. Appearance Fine granular powder
2. Color White
3.
REFERENCE: DSM (SINGAPORE)
SUNSET YELLOW

NO.
1. Appearance Orange yellow powder
2. Moisture 11.0 %
Solution comparison 0.0015 % (Beverage conc.) solution should be identical as compared
3.
with existing good one.
TARTRAZINE

Prepared By
Authorized By

NO.
1. Appearance Lemon yellow powder
2. Moisture 11.5 %
3.
REFERENCE: ROHA, INDIA
IDACOL SUNSET YELLOW

NO.
1. Appearance Orange yellow powder
2. Moisture 11.2 %
3.
IDACOL TARTRAZINE

Prepared By
Authorized By

NO.
1. Appearance Lemon yellow powder
2. Moisture 10.5 %
3.
IDACOL CARMOISINE

NO.
1. Appearance Red to maroon powder
2. Moisture 9.45 %
Solution comparison 0.00015 % (Beverage conc.) solution should be identical as
3.
REFERENCE: ROHA, INDIA
BETA CAROTENE

NO.
1. Appearance Red granular powder
2. Moisture 3.19 %
Solution comparison 0.018 % (fruit drink conc.) solution should be identical as compared
3.
REFERENCE: INHOUSE

Prepared By
Authorized By
GRINDSTED XANTHAN GUM 200

NO.
1. Appearance
2. Moisture 7.5 to 9.3
3. Viscosity (0.8%solution) 1300 to 1380 Cp, 60rpm, spindle L3
4. pH 7.0 to 7.1 (1% solution)
REFERENCE: DANISCO
MICRO CRYSTALLINE CELLULOSE POWDER

NO.
1. Physical State White powder
2. pH 5.5 to 7.0 ( 1% solution)
3. Moisture (%) Maximum 7.0 %
4. Bulk density 0.26 to 0.34 g/cc

Prepared By
Authorized By
REFERENCE: Mingtai Chemical co. ltd ( Taiwan)
CORN GRIT

NO.
1. Physical State Pale yellow granular powder
2. Particle size 425 to 1000 micron
3. Moisture (%) 10 to 11 %
4. Black particle Nil
REFERENCE: In house
MANGO PULP( ALLANA)

NO.
1. Appearance Uniform, homogenous consistency, free from fibers.
2. Color Bright yellow
3. Flavor & taste Typical of ripe fruits, free from any off flavor.
4. Acidity (%) 0.80 maximum
5. °Brix at 20°c Not less than 14.0

Prepared By
Authorized By

6. pH at 20°c 3.40 to 4.20
Black or brown Maximum 10
7.
specks/10 g
REFERENCE: Frigerio conserva allana limited.
MANGO PULP( GALLA)

NO.
1. Appearance Uniform texture, smooth
2. Color Golden yellow
3. Flavor & taste Characteristic natural ripe totapuri mango
4. Acidity (%) Minimum 0.40
5. °Brix at 20°c Not less than 14.0
6. pH at 20°c 3.6 to 4.00
Black or brown Maximum 10
7.
specks/10 g

Prepared By
Authorized By
IDENTIFICATION OF DEXTROSE
ADD FEW DROPS OF DEXTROSE SOLUTION (1 in 20 ml of water) IN HOT ALKALINE CUPRIC TARTRATE SOLUTION.
RED PPT IS APPEARED.
PREPARATION OF CUPRIC TARTRATE SOLUTION:
1. 34.66 g OF CUPRIC SULFATE DISSOLVE IN 500 ML OF WATER.

2. 173 g OF SODIUM POTTASIUM TARTRATE DISSSOLVE IN 500 ML OF WATER & ADD 50 g OF NaOH.
Mix the solution 1 & 2 in equal volume just before test.
IDENTIFICATION OF ACETIC ACID
Add FeCl3 Neutral solution to neutral solution of acetic acid. Red ppt is formed.

Prepared By
Authorized By

Preparation of neutral solution of FeCl3 solution:
Take about1 ml of ferric chloride solution. Add ammonium hydroxide solution until ppt is formed. Add
dil hydrochloric acid till ppt dissolved.
P3-STABICIP ABF
Molecular Formula: Formula weight: Equivalent Weight:

NO.
1. Physical State
2. Specific gravity
3. Purity (%)

Prepared By
Authorized By

Precautions:
1. Standardize KMnO4 Solution Before Titration.

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Analytical Test Procedure

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Analytical Test Procedure

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MEGHNA BEVERAGE LIMITED.

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 1

MATERIAL NAME: CITRIC ACID ANHYDROUS

SL Test Parameters Specification

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

Name Designation Sign & Date

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 2

MATERIAL NAME: TRI SODIUM CITRATE DIHYDRATE

SL Test Parameters Specification

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

Name Designation Sign & Date

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 3

MATERIAL NAME: SODIUM BENZOATE

SL Test Parameters Specification

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

MATERIAL NAME: COLA KIT PART-02 (PHOSPHORIC ACID)

Name Designation Sign & Date

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 4

SL Test Parameters Specification

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

2. Weighing Should Be Accurate

Name Designation Sign & Date

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 5

MATERIAL NAME: FERROUS SULFATE HEPTAHYDRATE

SL Test Parameters Specification

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

Name Designation Sign & Date

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 6

MATERIAL NAME: CALCIUM HYPOCHLORITE (BLEACHING POWDER)

SL Test Parameters Specification

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

Name Designation Sign & Date

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 7

MATERIAL NAME: CALCIUM OXIDE (LIME)

SL Test Parameters Specification

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

Name Designation Sign & Date

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 8

MATERIAL NAME: SODIUM CHLORIDE

SL Test Parameters Specification

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

MATERIAL NAME: ASCORBIC ACID (VITAMIN-C)

Name Designation Sign & Date

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 9

SL Test Parameters Specification

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

MATERIAL NAME: ASPARTAME

Name Designation Sign & Date

ANALYTICAL TEST PROCEDURE OF RAW MATERIAL Page - 10

SL Test Parameters Specification

METHOD OF ASSAY ANALYSIS

CALCULATION OF ASSAY PERCENTAGE

MATERIAL NAME: ACESULFAME-K