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  • Albumin Liquicolor: Photometric Colorimetric Test For Albumin BCG-Method

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    Maher
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    This document provides instructions for using a photometric colorimetric test to measure albumin concentration in serum or plasma using the bromocresol green method. The test involves adding a reagent containing bromocresol green to samples or a standard, which forms a colored complex with albumin. The absorbance is measured and used to calculate the albumin concentration. Sample results must be converted using a provided formula to match concentrations previously known for human samples. The test is linear up to 6.5 g/dL albumin and may be affected by interfering substances like bilirubin or hemoglobin.

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    Leaflet for Albumin for human diagnostic

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    Albumin

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    This document provides instructions for using a photometric colorimetric test to measure albumin concentration in serum or plasma using the bromocresol green method. The test involves adding a reagent containing bromocresol green to samples or a standard, which forms a colored complex with albumin. The absorbance is measured and used to calculate the albumin concentration. Sample results must be converted using a provided formula to match concentrations previously known for human samples. The test is linear up to 6.5 g/dL albumin and may be affected by interfering substances like bilirubin or hemoglobin.

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    © All Rights Reserved
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    742 views1 page

    Albumin Liquicolor: Photometric Colorimetric Test For Albumin BCG-Method

    Uploaded by

    Maher
    This document provides instructions for using a photometric colorimetric test to measure albumin concentration in serum or plasma using the bromocresol green method. The test involves adding a reagent containing bromocresol green to samples or a standard, which forms a colored complex with albumin. The absorbance is measured and used to calculate the albumin concentration. Sample results must be converted using a provided formula to match concentrations previously known for human samples. The test is linear up to 6.5 g/dL albumin and may be affected by interfering substances like bilirubin or hemoglobin.

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    © All Rights Reserved
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    of 1

    Albumin liquicolor Conversion Factor

    To convert the obtained results into the previously known concentrations


    Photometric Colorimetric Test for Albumin of HUMAN apply the formula below:
    BCG-Method Concentration (CRM 470) x 1.218 = Concentration (Human)

    Package Size Performance Characteristics


    [REF] 10560 1 x 1000 ml Complete test kit Linearity
    156004 4 x 100 ml Complete test kit The test is linear up to an albumin concentration of 6,5 g/dl or 65 g/l.
    [IVD] Dilute samples with a higher concentration 1 + 1 with physiological saline
    (0.9%). Multiply the result by 2.
    Method 1, 2 Typical performance data can be found in the Verification Report,
    Bromocresol green forms with albumin in citrate buffer a coloured accessible via:
    complex. The absorbance of this complex is proportional to the albumin www.human.de/data/gb/vr/su-albu.pdf or
    concentration in the sample.
    www.human-de.com/data/gb/vr/su-albu.pdf
    Contents
    [RGT] 4 x 100 or 1 x 1000 ml Colour reagent Normal Range in Serum or Plasma
    Citrate buffer (pH 4.2) 30 mmol/l 3.8 - 5.1 g/dl or 38 - 51 g/l
    Bromocresol green 260 μmol/l
    Quality Control
    [STD] 1 x 3 ml Standard All control sera with albumin values determined by this method may be
    Albumin 4 g/dl or 40 g/l employed.
    Sodium azide 0.095 %
    We recommend to use our HumaTrol quality control sera based on animal
    [STD] is standardised against the certified reference material CRM- serum or our SERODOS based on human serum.
    DA470k/IFCC.
    Automation
    Reagent Preparation
    Proposals to apply the reagents on analysers are available on request.
    [RGT] and [STD] are ready for use. Each laboratory has to validate the application in its own responsibility.
    Reagent Stability Notes
    [RGT] and [STD] are stable up to the given expiry date when stored at 1. The test is not influenced by bilirubin values up to 20 mg/dl. Each
    2...25°C. 100 mg/dl hemoglobin will represent an albumin increase of 0.1 g/dl,
    Contamination after opening must be avoided. so cross hemolysis should be avoided.
    2. Cross hemolysis and marked lipemia will interfere. A sample blank
    Specimen
    must be determined by pipetting 10 μl sample to 1000 μl physiological
    Serum, heparinised or EDTA-plasma saline and measured against dist. water. The absorbance of the sample
    Stability in serum: at 2...8°C up to 1 month blank has to be subtracted from the absorbance of the sample.
    at 15...25°C up to 1 week 3. The standard contains sodium azide. Do not swallow. Avoid contact
    with skin and mucous membranes.
    Assay
    Wavelength: Hg 546 nm, 578 nm References
    Optical path: 1 cm 1. Rodkey F. L., Clin. Chem. 10, 606 (1964)
    Temperature: 20...25°C 2. Doumas B. T. et al., Clin. Chim. Acta 31, 87 (1971)
    Measurement: Against reagent blank. Only one reagent blank per series
    is required. SU-ALBU INF 156001 GB 06-2012-17 |
    Pipetting Scheme
    Pipette into cuvettes Reagent blank Sample or [STD]
    Sample/ [STD] --- 10 μl
    [RGT] 1000 μl 1000 μl
    Mix, incubate for 5 min. at 20...25°C. Measure the absorbance of the
    sample and the standard against the reagent blank within 30 min. (ΔA).

    Calculation of the Albumin Concentration


    ΔAsample
    C = 4 x ⎯⎯⎯⎯⎯⎯ [g/dl]
    ΔA[STD]
    or
    ΔAsample
    C = 40 x ⎯⎯⎯⎯⎯⎯ [g/l]
    ΔA[STD]

    Human Gesellschaft für Biochemica und Diagnostica mbH


    Max-Planck-Ring 21 · 65205 Wiesbaden · Germany
    Telefon +49 6122-9988-0 · Telefax +49 6122-9988-100 · e-Mail [email protected]

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