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  • WBC Count: Hemacytometer

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    rona hilario
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    The document describes how to perform a white blood cell (WBC) count using a hemocytometer, which is a thick glass slide with an indented chamber. The hemocytometer, also called a Neubauer chamber, contains a grid with squares that allow counting of cells within a known area and volume. To get a WBC count, white blood cells in one of the corner squares are counted and the number is multiplied by a dilution factor and divided by the area and depth counted to calculate the concentration of WBCs per volume of blood.

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    hema

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    WBC-COUNT

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    The document describes how to perform a white blood cell (WBC) count using a hemocytometer, which is a thick glass slide with an indented chamber. The hemocytometer, also called a Neubauer chamber, contains a grid with squares that allow counting of cells within a known area and volume. To get a WBC count, white blood cells in one of the corner squares are counted and the number is multiplied by a dilution factor and divided by the area and depth counted to calculate the concentration of WBCs per volume of blood.

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    © All Rights Reserved
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    47 views5 pages

    WBC Count: Hemacytometer

    Uploaded by

    rona hilario
    The document describes how to perform a white blood cell (WBC) count using a hemocytometer, which is a thick glass slide with an indented chamber. The hemocytometer, also called a Neubauer chamber, contains a grid with squares that allow counting of cells within a known area and volume. To get a WBC count, white blood cells in one of the corner squares are counted and the number is multiplied by a dilution factor and divided by the area and depth counted to calculate the concentration of WBCs per volume of blood.

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    © All Rights Reserved
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    of 5

    WBC COUNT

    HEMACYTOMETER

     The hemocytometer is a device originally designed and usually used for counting blood cells.
     The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass
    microscope slide with a rectangular indentation that creates a chamber.

    Materials
     The necessary elements to perform a cell count with Neubauer chamber are as followS:
    A. cellular dilution to measure
    B. hemocytometer, or Neubabuer chamber
    C. optical microscope
    D. cover glass
    E. pippette / micropippete with disposable tips.
    F. dilutlon buffer / PBS (if needed)
    THE NEUBABUER CHAMBER, OR HEMOCYTOMETER

    1. The Neubauer chamber is a thick crystal slide with the size


    of a glass slide. (30 x 70 mm and 4 mm thickness)
    2. In a simple counting chamber, the central area is where
    cell counts are performed.

    Double chambers are most common than simple chamber. In this case, the chamber has two counting
    areas than can be loaded independently.

    Neubauer chamber's counting grid is 3 mmx3 mm in size. The grid has 9 square subdivisions of width
    1mm.

    Counting Grid
    IMPROVED NEUBAUER CHAMBER COUNTING AREA
    Counting Grid Areas Are present on central platform of chamber

    Counting Grid
     Each scale is 3mm wide and 3mm
    long (9mm2 total area)
     The whole scale is divided into 9
    big squares.
     Each squaree 1mm long and 1mm
    wide. (1mm area)
    In case of blood cell counting, the squares placed at the corners are used for white cell counting. Since
    their concentration is lower than red blood cells a larger area is required to perform the cell count

     The central square is used for platelets and red cells. This square is split in 25 squares of width
    0.2 mm (200um).
     Each one of the 25 central squares is subdivided in 16 small squares
    WBC count = cells counted X dilution factor
    Area counted (mm2) X depth

    = 96 X 20
    4 X 0.1

    = 4800/mm3 or
    4 800/µL or
    4.8 X 10^3/µL or
    4.8 X 10^9/L

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