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  • 3E Enzymes Worksheet

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    Timothy Handoko
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    This document contains a worksheet on enzymes for secondary 3 science students. It includes multiple choice and structured questions about enzymes. Some key points are: - Enzymes are proteins that catalyze chemical reactions but are not changed by the reactions. They can be destroyed by high temperatures. - Enzymes like amylase act as a "key" that fits into the "lock" of its substrate, like starch. Amylase converts starch to maltose. - Enzyme activity peaks at an optimum temperature and then declines at higher temperatures as the enzymes denature. - Fresh pineapple juice contains an enzyme that can break down proteins and digest meat, explaining why pineapple is sometimes served with

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    This document contains a worksheet on enzymes for secondary 3 science students. It includes multiple choice and structured questions about enzymes. Some key points are: - Enzymes are proteins that catalyze chemical reactions but are not changed by the reactions. They can be destroyed by high temperatures. - Enzymes like amylase act as a "key" that fits into the "lock" of its substrate, like starch. Amylase converts starch to maltose. - Enzyme activity peaks at an optimum temperature and then declines at higher temperatures as the enzymes denature. - Fresh pineapple juice contains an enzyme that can break down proteins and digest meat, explaining why pineapple is sometimes served with

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    33 views4 pages

    3E Enzymes Worksheet

    Uploaded by

    Timothy Handoko
    This document contains a worksheet on enzymes for secondary 3 science students. It includes multiple choice and structured questions about enzymes. Some key points are: - Enzymes are proteins that catalyze chemical reactions but are not changed by the reactions. They can be destroyed by high temperatures. - Enzymes like amylase act as a "key" that fits into the "lock" of its substrate, like starch. Amylase converts starch to maltose. - Enzyme activity peaks at an optimum temperature and then declines at higher temperatures as the enzymes denature. - Fresh pineapple juice contains an enzyme that can break down proteins and digest meat, explaining why pineapple is sometimes served with

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    of 4

    Guangyang Secondary School

    Secondary 3 Science (Biology)


    Enzymes Worksheet
    Name: _________________________________ ( ) Date: ___________

    Class: ___________

    ______________________________________________________________________________

    MULTIPLE-CHOICE QUESTIONS

    1. Which statement about enzymes is correct?

    A. They are all proteins.


    B. They are changed by the chemical reactions they control.
    C. They are destroyed by temperatures below 10⁰C.
    D. They are found only in the alimentary canal.
    ( )

    2. 4 test tubes were set up as shown below.

    What is the result?

    Tube number
    1 2 3 4
    A. clear clear clear clear
    B. clear cloudy cloudy clear
    C. cloudy cloudy clear cloudy
    D. cloudy clear cloudy clear
    ( )

    1
    3. Starch is digested to maltose by the enzyme amylase. According to the lock and key
    hypothesis, which is the “key” and which is the “lock”?

    key lock
    A. amylase maltose
    B. amylase starch
    C. starch amylase
    D. starch maltose

    ( )

    4. Which graph shows how an enzyme catalysed reaction in the alimentary canal varies
    with temperature?

    ( )

    5. An experiment was set up to investigate the action of amylase on a 1.0g cube of potato.
    The cube of potato was submerged in a test tube with an amylase solution. The potato
    contains starch.
    After 15 minutes at 20⁰C, 0.25g of starch was converted to sugar.
    The experiment is then repeated at 30⁰C. How much starch would be converted to
    sugar? (Hint: Activity of enzyme doubles for every 10⁰C increase in temperature)

    A. 0.15g C. 0.50g
    B. 0.25g D. 1.0g
    ( )

    2
    STRUCTURED QUESTIONS

    6. A student who had seen a cook place slices of fresh pineapple on top of meat before
    cooking it, decided to carry out some experiments using fresh pineapple juice.

    A fat-free milk agar was prepared and poured into a sterilized petri dish. After it had
    cooled and set, five holes were made in the agar and each hole was filled with a
    different substance as shown in Fig 2.1.

    Key:

    1 – distilled water Petri dish


    1 2
    2 – solution of lactase (enzyme that
    digests milk sugar)
    Cloudy milk agar
    3 – solution of protease
    5

    4 - boiled pineapple juice


    4 3
    5 – fresh pineapple juice

    Fig 2.1

    The petri dish was then kept at 40⁰C for 2 hours. The results showed that holes 3 and 5 became
    clear areas.

    a) Explain why the petri dish was kept at 40⁰C. [2]

    ________________________________________________________________________

    ________________________________________________________________________

    b) Explain the results obtained for each of the holes 1 – 5. [5]

    Hole 1: __________________________________________________________________

    Hole 2: __________________________________________________________________

    Hole 3: __________________________________________________________________

    Hole 4: __________________________________________________________________

    Hole 5: __________________________________________________________________

    3
    c) Using the results of this experiment, suggest reasons why cooks place fresh pineapple
    on meat before cooking it. [2]

    ________________________________________________________________________

    ________________________________________________________________________

    7. The graph below (Fig2.2) shows the results of temperature on a chemical reaction which
    is controlled by enzymes.

    Fig. 2.2

    a) What is the optimum temperature of this enzyme? [1]

    _____________________________________________________________________

    b) Explain the shape of the graph after 40⁰C. [2]

    _____________________________________________________________________

    _____________________________________________________________________

    _____________________________________________________________________

    c) It was later found out that the enzyme digests carbohydrates. Name this type of
    enzyme. [1]

    _____________________________________________________________________

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