Lab Topic 13 Paternity Testing
Lab Topic 13 Paternity Testing
Lab Topic 13 Paternity Testing
itself, but the number of times the sequence is repeated. You will inherit ½ of the TH01 from your father
LAB TOPIC 13: PATERNITY TESTING AND READING & INTERPRETATION and the other half from your mother. This means that each individual will have two numbers. In the right image,
Lecturer: Sir Niño Paolo Tan, RMT, MTMRS (c) the father has a TH01 of 8 and 11, meaning, in one part of his chromosome, the TCAT sequence is repeated 11
times (this number was also inherited from his parents). Still referring to the right image, the mother has 9 and 13,
DNA FINGERPRINTING: PARENTAGE AND KINSHIP TESTING while the child is 9 and 11. You can see that 9 is the same for both the mother and the child, hence, 9 is the
▪ another application of DNA fingerprinting and is still part of the civil investigation maternal marker which can be ruled out so that we can focus on the nonmaternal marker of the child which is 11.
▪ part from solving crimes, DNA evidences has been used to solve other issues, such as family relatedness To establish a match, the 11 marker must be present also in the father which can be seen on the right image. With
▪ Genetic markers are heritable, a parent passes on 1 copy of their marker genes to a child. This leads to the this, we can genetically link the DNA of the child to the DNA of the father. In conclusion, this child is really the
similarity of profile between related individuals. offspring of the father.
▪ the greater the degree of relatedness, the more likely it is that profile will be similar
▪ useful in parentage disputes and missing persons cases
▪ the traits that are being shown phenotypically (between a parent and a child) is similar between related
individuals since they are both phenotypically and genetically the same
▪ the greater the degree of relatedness, the more likely it is that the profile will be similar (the closer the familial
relation is, the more similar the DNA profile will be): that is why DNA fingerprinting technology is very useful in
parentage disputes and in missing persons cases
▪ identical by descent (ibd)
o an allele that investigators take a look into when they are trying to solve this kind of cases (e.g. parentage
disputes)
o 2 copies, or alleles, of the same genetic marker that have descended from the same ancestral allele
o 2 individuals who belong in the same family share (0,1,2) pairs of ibd alleles
REFERENCES
▪ Jamieson, A. & Bader, S. (2016). Introduction to Forensic Genetics. In A Guide to Forensic DNA Profiling John
Wiley & Sons Ltd.
▪ BIO RAD Crime Scene Investigation Procedure Manual
▪ Dash, H.R., et. al. (2018). DNA Fingerprinting: Advancements and Future Endeavors. Springer Nature Singapore
TH01 is one of the 13 alleles we do testing on when we do DNA testing on forensic cases. TH01 represents the Pte Ltd.
microsatellite TCAT (repeat). The TCAT sequence (left image) was repeated 6, 7, and 8 times. What varies is not the
AIRAH M.