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Tobacco and Tobacco Products - Determination of Reducing and Total Sugars
Tobacco and Tobacco Products - Determination of Reducing and Total Sugars
ICS 65.160
© BIS 2018
FOREWORD
This Indian Standard was adopted by the Bureau of Indian Standards, after the draft finalized by the Tobacco and
Tobacco Products Sectional Committee had been approved by the Food and Agriculture Divisional Council.
The chemical properties of leaf tobacco are influenced by agricultural practices, soil type and nutrients, weather
conditions, stalk position and curing procedures. A change in any of these factors can markedly alter the chemical
composition of tobacco leaf and thus affect the quality of tobacco products.
The total and reducing sugar contents of tobacco are important indicators of the quality of tobacco raw materials and
finished tobacco products. This standard will help in getting uniform and reproducible results while determining
the total and reducing sugar contents in tobacco and tobacco products and thereby a consistent quality product.
In reporting the result of a test or analysis made in accordance with this standard, if the final value, observed or
calculated, is to be rounded off, it shall be done in accordance with IS 2 : 1960 ‘Rules for rounding off numerical
values ( revised )’.
Free Standard provided by BIS via BSB Edge Private Limited to RAJ GUPTA -
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IS 17010 : 2018
Indian Standard
TOBACCO AND TOBACCO PRODUCTS —
DETERMINATION OF REDUCING
AND TOTAL SUGARS
1
Free Standard provided by BIS via BSB Edge Private Limited to RAJ GUPTA -
Faridabad([email protected]) 223.190.126.83 [for non-commercial use only].
IS 17010 : 2018
2
Free Standard provided by BIS via BSB Edge Private Limited to RAJ GUPTA -
Faridabad([email protected]) 223.190.126.83 [for non-commercial use only].
IS 17010 : 2018
0.005 N thiosulphate solution till the solution becomes V1 = volume of original sample extract (100 ml);
pale yellow. Add 100 µl starch indicator solution and V2 = volume of diluted sample extract (50 ml);
complete titration. The end point is blue to colourless.
V3 = volume of original sample extract used for
9.2 Carry out 3 blank determinations using 5 ml of dilution (10 ml); and
water in place of sample extract 9.1. V4 = volume of sample used for titration (5 ml).
9.3 Subtract titre values of test solution from that of 10.2 In case the titre values exceeds 17.9 ml, the
blank and calculate the amount of glucose corresponding following formula may be used for calculating mg
to the titre difference from the formula. glucose
Glucose, mg = 0.112 × (ml of 0.005 N sodium
10 CALCULATIONS
thiosulphate) + 0.04 ml of 0.005 N Sodium Thiosulphate
10.1 Calculate sugars (as glucose), percent by dry mass = (Blank titre value – Sample titre value)
from the following formula:
11 REPORTING
Sugars (as glucose), percent by
The report will carry the following:
G × V1 × V2 × 100 100
=
dry mass × a) Full identity of the sample;
W × 1000 × V3 × V4 (100 − M )
b) Date of test;
Where, c) Moisture content, percent of sample as received;
G = glucose, in mg (see Table 1); d) Reducing sugar content, percent on dry basis;
W = weight of sample, in g; e) Total sugar content, percent on dry basis; and
M = percent moisture content; f) Non-reducing sugar, percent on dry basis.
3
Free Standard provided by BIS via BSB Edge Private Limited to RAJ GUPTA -
Faridabad([email protected]) 223.190.126.83 [for non-commercial use only].
Free Standard provided by BIS via BSB Edge Private Limited to RAJ GUPTA -
Faridabad([email protected]) 223.190.126.83 [for non-commercial use only].
Free Standard provided by BIS via BSB Edge Private Limited to RAJ GUPTA -
Faridabad([email protected]) 223.190.126.83 [for non-commercial use only].
BIS is a statutory institution established under the Bureau of Indian Standards Act, 2016 to promote harmonious
development of the activities of standardization, marking and quality certification of goods and attending to
connected matters in the country.
Copyright
BIS has the copyright of all its publications. No part of these publications may be reproduced in any form without
the prior permission in writing of BIS. This does not preclude the free use, in the course of implementing the
standard, of necessary details, such as symbols and sizes, type or grade designations. Enquiries relating to
copyright be addressed to the Director (Publications), BIS.
Amendments are issued to standards as the need arises on the basis of comments. Standards are also reviewed
periodically; a standard along with amendments is reaffirmed when such review indicates that no changes are
needed; if the review indicates that changes are needed, it is taken up for revision. Users of Indian Standards
should ascertain that they are in possession of the latest amendments or edition by referring to the latest issue of
‘BIS Catalogue’ and ‘Standards: Monthly Additions’.
This Indian Standard has been developed from Doc No.: FAD 04 (2786).