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  • 4.8.09 AOAC Official Method 975.08 Fluorine in Animal Feed: Distillation Method First Action 1975 Final Action 1976

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    Nguyễn Khang Luân
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    This document describes a method for determining fluorine in animal feed using an ion selective electrode. It involves preparing standard fluoride solutions of varying concentrations from a stock solution. Test samples are prepared by dissolving the feed sample and adding reagents. The fluoride electrode is used to measure the mV of standard and test solutions, from which a standard curve is constructed to determine the μg/mL of fluoride in the test sample and calculate the percentage of fluorine.

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    975_08

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    This document describes a method for determining fluorine in animal feed using an ion selective electrode. It involves preparing standard fluoride solutions of varying concentrations from a stock solution. Test samples are prepared by dissolving the feed sample and adding reagents. The fluoride electrode is used to measure the mV of standard and test solutions, from which a standard curve is constructed to determine the μg/mL of fluoride in the test sample and calculate the percentage of fluorine.

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    244 views1 page

    4.8.09 AOAC Official Method 975.08 Fluorine in Animal Feed: Distillation Method First Action 1975 Final Action 1976

    Uploaded by

    Nguyễn Khang Luân
    This document describes a method for determining fluorine in animal feed using an ion selective electrode. It involves preparing standard fluoride solutions of varying concentrations from a stock solution. Test samples are prepared by dissolving the feed sample and adding reagents. The fluoride electrode is used to measure the mV of standard and test solutions, from which a standard curve is constructed to determine the μg/mL of fluoride in the test sample and calculate the percentage of fluorine.

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    © All Rights Reserved
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    of 1

    4.8.09 solve and dilute to volume with H2O, and mix.

    Store in plastic
    AOAC Official Method 975.08 bottle. (2) Intermediate solution I.—100 µg/mL. Pipet 20 mL
    Fluorine in Animal Feed stock solution into 100 mL volumetric flask, dilute to volume
    Distillation Method with H2O, and mix. (3) Intermediate solution II.—10 µg/mL.
    First Action 1975 Pipet 2 mL stock solution into 100 mL volumetric flask, dilute to
    Final Action 1976 volume with H2O, and mix. (4) Working solutions.—Pipet 3, 5,
    Determine as in 944.08, especially E (see 9.2.11). and 10 mL intermediate solution II and 5 and 10 mL intermediate
    solution I into five 100 mL volumetric flasks to prepare 0.3, 0.5,
    Ion Selective Electrode Method 1.0, 5.0, and 10 µg/mL F working solutions, respectively. To each
    First Action add 10.0 mL 1N HCl, 25.0 mL NaCH3COO⋅3H2O solution, (a),
    Final Action
    and 25.0 mL sodium citrate solution, (b). Dilute to volume with
    H2O and mix.
    A. Apparatus
    C. Preparation of Sample
    (a) Electrodes.—Fluoride ion selective electrode
    (Model 94-09 [replaced by 94-09-BN], Orion Research, Inc., or Accurately weigh well-mixed test portion containing ca 400 µg F
    equivalent) and single junction calomel reference electrode, plas- into 200 mL volumetric flask. Pipet in 20 mL 1N HCl and stir 20 min
    tic sleeve-type (Model 90-01 [replaced by 90-01-00], Orion Re- at high speed on magnetic stirrer. Add 50.0 mL NaCH3COO solu-
    search, Inc., or equivalent). tion, C(a), and 50.0 mL sodium citrate solution, C(b), to test solu-
    (b) Magnetic stirrer.—With 4 cm (1 12″) Teflon-coated stirring tion. Dilute to volume with H2O and mix.
    bar. Use mat to insulate sample from motor heat. D. Determination
    (c) pH meter.—Corning digital Model 112 (Corning Scientific Connect F and reference electrodes to pH meter, place electrodes
    Instruments, 63 North St, Medfield, MA 02052, or equivalent). in low concentration F solution, and warm up pH meter. Pour
    50–70 mL standard and corresponding test solutions into separate
    B. Reagents
    100 mL beakers. Place electrodes in each solution and while stirring
    (Deionized H2O may be used.) with magnetic stirrer at constant rate, read mV of standard and un-
    ( a ) S o d i u m a c e t a t e s o l u t i o n. — 3 M . D i s s o l v e 4 0 8 g known solutions. Rinse and blot off electrodes and stirring bar be-
    NaCH3COO⋅3H2O with H2O in 1 L volumetric flask. When solu- tween solutions. Construct standard curve on 3 cycle
    tion warms to room temperature, dilute to volume with H2O. Ad- semilogarithmic paper. Read µg/mL F of sample solution from stan-
    just to pH 7.0 with few drops CH3COOH. dard curve.
    (b) Sodium citrate solution.—1.32M. Dissolve 222 g sodium
    citrate⋅2H2O with ca 250 mL H2O in 1 L volumetric flask. Add F, % =
    28 mL HClO4, dilute to volume, and mix. µg/mL F × test solution, mL × 10–6 × 100/test portion, g
    ( c ) F l u o r i d e s t a n d a r d s o l u t i o n s . — ( 1 ) S t o c k s o l u-
    Reference: JAOAC 58, 477(1975).
    tion.—500 µg/mL (mg/L). Accurately weigh 1.105 g NaF (re-
    agent grade, dried 4 h at 100°C) into 1 L volumetric flask. Dis- CAS-7782-41-4 (fluorine)

     2000 AOAC INTERNATIONAL

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