Cambridge International AS & A Level

BIOLOGY 9700/01
Paper 1 Multiple Choice For examination from 2022
SPECIMEN PAPER 1 hour 15 minutes

You must answer on the multiple choice answer sheet.

*0123456789*

You will need: Multiple choice answer sheet

Soft clean eraser
Soft pencil (type B or HB is recommended)

INSTRUCTIONS
● There are forty questions on this paper. Answer all questions.
● For each question there are four possible answers A, B, C and D. Choose the one you consider correct
and record your choice in soft pencil on the multiple choice answer sheet.
● Follow the instructions on the multiple choice answer sheet.
● Write in soft pencil.
● Write your name, centre number and candidate number on the multiple choice answer sheet in the
spaces provided unless this has been done for you.
● Do not use correction fluid.
● Do not write on any bar codes.
● You may use a calculator.

INFORMATION
● The total mark for this paper is 40.
● Each correct answer will score one mark.
● Any rough working should be done on this question paper.

This document has 18 pages. Blank pages are indicated.

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1 Which definition of the magnification of a drawing of a leaf is correct?

A the actual size of the leaf multiplied by the magnification of the microscope

B the measured difference in size between the leaf and a drawing of the leaf

C the increase in size of the leaf when observed using a microscope

D the size of the drawing of the leaf divided by the actual size of the leaf

2 The electron micrograph shows part of two cells.

Which labelled features identify these cells as eukaryotic?

A W, X and Y B W and X only C W only D X only

3 Plant cells are stained and viewed using a student microscope. The light source is natural light.

What could be clearly visible at u400 magnification?

A cristae of mitochondria

B grana of chloroplasts

C nucleoli

D ribosomes

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4 Which lengths are equivalent to 1 Pm?

1 1000 mm

2 0.001 nm

3 0.001 mm

4 1 000 000 nm

5 0.01 mm

6 1000 nm

A 1 and 4 B 2 and 5 C 3 and 4 D 3 and 6

5 Some secretory cells synthesise and release glycoproteins.

What is the correct sequence of some of the events that occur in a secretory cell?

1 exocytosis

2 products accumulate in secretory vesicles

3 mRNA binds to ribosomes

4 synthesis of glycoproteins

A 3, 4, 1, 2 B 3, 4, 2, 1 C 4, 3, 1, 2 D 4, 3, 2, 1

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6 A scientist used a blender to break open a sample of animal cells and release the organelles.

The extracted mixture was spun in a centrifuge at a low speed. The heaviest type of organelle
sank to the bottom of the centrifuge tube to form a solid pellet, pellet 1.

liquid above
pellet

solid pellet

The liquid above pellet 1 was poured into a clean centrifuge tube and spun at a higher speed. The
next heaviest organelle sank to the bottom to form a solid pellet, pellet 2.

The scientist repeated this procedure twice more to obtain pellet 3 and pellet 4, each containing a
single type of organelle.

What is the function of the type of organelle extracted in pellet 3?

A digestion of old cell organelles

B production of ATP by respiration

C synthesis of mRNA

D synthesis of protein

7 What is the general formula for amylose?

A (C5H10O5)n B (C5H10O6)n C (C6H12O6)n D (C6H10O5)n

8 Which statement describes how the molecular structure of starch is suited to its function?

A Amylose has a branched structure and amylopectin is coiled to give a compact molecule for
transport.

B In the breakdown of amylose and amylopectin, many condensation reactions release stored
energy.

C In the formation of amylose and amylopectin, many hydrolysis reactions allow the release of
stored energy.

D Amylose and amylopectin form a complex that is insoluble and so does not affect the water
potential of the cell.

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9 Cows and whales are mammals that produce milk to feed their babies. Newborn whales grow
faster than newborn cows. Milk contains fatty acids with a range of chain lengths.

The table shows the percentage of fatty acids of different chain lengths in cow milk and whale
milk.

chain length of fatty percentage of fatty acids in milk

acids / number of
cow whale
carbon atoms
4–12 22.2 0.0
14 10.6 13.8
16 25.5 27.9
18 40.1 29.4
> 18 1.6 28.9

Which statement about the ratio of short fatty acids (4–16 carbon atoms) to long fatty acids
(18 or more carbon atoms) in the milk of cows and whales is correct?

A The ratio in cow milk is higher because newborn cows need more energy than newborn
whales.

B The ratio in cow milk is lower because newborn cows need less energy than newborn whales.

C The ratio in whale milk is higher because newborn whales need less energy than newborn
cows.

D The ratio in whale milk is lower because newborn whales need more energy than newborn
cows.

10 The structure of phospholipids includes:

1 polar phosphate heads

2 hydrophobic fatty acid chains

3 saturated fatty acid chains.

What are essential for the formation of a phospholipid bilayer in a cell surface membrane?

A 1 and 2 B 1 and 3 C 2 and 3 D 3 only

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11 The diagram shows the molecular structure of a peptide.

O NH2
C

CH2 H O CH3
H
H C N C C OH
N C C N C
H H
H O CH2 H O

OH

Which molecules would result from the complete hydrolysis of the peptide?

O NH2
C

CH2

H CH2 H H H CH3

A N C COOH N C COOH N C COOH

H H H
H CH2 H

O NH2 OH
C

H CH2 H H H CH3

B N C COOH N C COOH N C COOH

H H H
H H C OH H

CH3
O NH2
C

H CH2 H H H CH3

C N C COOH N C COOH N C COOH

H H H
H CH2 H

NH2 OH
O
C

H CH2 H H H H

D N C COOH N C COOH N C COOH

H H H
H CH2 H

OH

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12 The diagram shows a haemoglobin molecule.

β-globin chain
3

α-globin chain

4
2

α-globin chain

β-globin chain

Which row identifies the different parts of the molecule?

1 2 3 4
A D-helix E-pleated oxygen binding hydrophobic
sheet site amino acids
B oxygen binding hydrophilic D-helix hydrophobic
site amino acids amino acids
C haem group hydrophobic D-helix hydrophilic
amino acids amino acids
D hydrophobic E-pleated haem atom oxygen binding
amino acids sheet site

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13 A student investigated the effect of enzyme concentration on the rate of hydrolysis of protein in
milk.

When the enzyme and milk were mixed, the protein was hydrolysed and the mixture changed
from cloudy to clear.

The student investigated five different enzyme concentrations and recorded the time taken for the
mixture to become clear.

What is an appropriate control for this investigation?

A Carrying out an experiment where the enzyme solution is replaced with water.

B Carrying out each experiment in a thermostatically controlled water-bath at 35 °C.

C Repeating each experiment three times for each of the five enzyme concentrations.

D Using the same volume of enzyme solution for each of the five experiments.

14 What determines the specificity of an enzyme?

1 the covalent and other bonding between R groups of the polypeptide

2 the optimum pH of the enzyme

3 the covalent peptide bonds between amino acids of the polypeptide

4 the shape of the substrate molecule

A 1, 2, 3 and 4 B 1 and 3 only C 1 only D 2, 3 and 4 only

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15 The graph shows the effect of temperature on the rate at which the enzyme in a biological washing
powder digests and removes fruit juice stains.

rate of
reaction

T
temperature

Which statements explain the shape of the graph at temperatures higher than T?

1 Bonds are broken between the R groups of the amino acids in the polypeptide chains of
the enzyme.

2 There are more collisions between the enzyme and its substrate.

3 The tertiary structure of the enzyme is altered.

4 The shapes of the active site and the substrate are no longer complementary.

A 1, 2 and 3 B 1, 2 and 4 C 1, 3 and 4 D 2, 3 and 4

16 Which statement describes carrier proteins in cell surface membranes?

A Carrier proteins are found on the outer surface of the membranes allowing cell recognition.

B Carrier proteins are involved in moving substances through the membranes by either active
transport or passive transport.

C Carrier proteins allow the binding of ligand molecules, which causes changes within cells.

D Carrier proteins are involved in moving substances through the membranes by passive
transport through water-filled pores.

17 What happens to a typical bacterium when it is placed in surroundings that have a higher water
potential than the water potential inside the cell?

A The bacterium bursts because the cell wall has no structural function.

B The bacterium dies because water leaves the cell by osmosis.

C There is no change because the cell wall is impermeable to water.

D There is a net movement of water into the bacterium.

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18 By which process do hydrogencarbonate ions leave red blood cells?

A active transport

B endocytosis

C facilitated diffusion

D phagocytosis

19 In an experiment, fluorescent dyes were attached to proteins on the outer surface of cell surface
membranes. Fluorescent dyes of one colour were attached to proteins of a living human cell and
fluorescent dyes of a different colour were attached to proteins of a living mouse cell.

The human cell and the mouse cell were then fused to form a hybrid cell.

At first, the proteins attached to different fluorescent dyes remained separate, but after 40 minutes
the proteins were distributed randomly across the hybrid cell surface membrane.

What does this experiment show?

A Proteins are found only on the outer surface of cell surface membranes.

B Proteins in the outer layer of a bilayer do not penetrate into the inner layer.

C Proteins move freely in the phospholipids of a bilayer.

D The cell surface membranes of the two cells are bilayers.

20 The diagram shows the mitotic cell cycle.

During which phase is DNA replicated?

cytokinesis
D
C

B
A

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21 Bacterial cells divide by a process called binary fission.

Which macromolecules must be synthesised for binary fission?

1 cell membrane proteins and RNA

2 DNA and peptidoglycan

3 enzymes and cellulose

A 1, 2 and 3 B 1 and 2 only C 2 and 3 only D 3 only

22 The electron micrograph shows a group of human chromosomes.

W
Y

Which label is correct for each of the structures labelled W, X and Y?

W X Y
A centriole centromere chromatid
B centriole centromere microtubule
C centromere telomere chromatid
D centromere telomere microtubule

23 Which statement about the behaviour of chromosomes during mitosis is correct?

A They attach to the spindle fibres to contain them within the nucleus.

B They condense to prevent further translation of genes.

C They reach the poles of the cell and begin to uncoil.

D They replicate to produce sufficient DNA to form two new nuclei.

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24 Gene mutations can occur by substitution, deletion or insertion.

What is the smallest part of a DNA molecule that can be changed by a gene mutation?

A base

B codon

C gene

D nucleotide

25 Which statements about mRNA are correct?

1 mRNA can form base pairs.

2 mRNA can form hydrogen bonds.

3 mRNA is single stranded.

A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

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26 The table shows the role of four different proteins involved in DNA replication.

protein helicase topoisomerase single-strand DNA polymerase

binding protein
role unwinds the breaks and rejoins binds to separated synthesises strand
parental DNA the DNA strands DNA strands to of DNA
double helix stabilise them

Which row shows the functions of these proteins?

helicase topoisomerase single-strand DNA polymerase

binding protein
A adds DNA prevents original enables tension makes strands
nucleotides to the strands re-forming caused by available as
3c end of a growing complementary unwinding to be templates
polynucleotide base pairs released
strand
B enables tension prevents original makes strands adds DNA
caused by strands re-forming available as nucleotides to the
unwinding to be complementary templates 3c end of a growing
released base pairs polynucleotide
strand
C enables tension makes strands adds DNA prevents original
caused by available as nucleotides to the strands re-forming
unwinding to be templates 3c end of a growing complementary
released polynucleotide base pairs
strand
D makes strands enables tension prevents original adds DNA
available as caused by strands re-forming nucleotides to the
templates unwinding to be complementary 3c end of a growing
released base pairs polynucleotide
strand

27 Which type of sugar and which type of bond are found in a DNA molecule?

type of sugar type of bond

A disaccharide glycosidic
B disaccharide phosphodiester
C monosaccharide peptide
D monosaccharide hydrogen

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28 The diagrams represent transverse sections of three plant organs.

2
4 5

6
1 3
root stem leaf

Which row is correct for phloem?

root stem leaf

A 1 3 5
B 1 4 6
C 2 3 6
D 2 4 5

29 Sucrose moves from a phloem sieve tube element into a root cell.

Which changes to the water potential and the volume of liquid in the phloem sieve tube element
are correct?

water potential volume of liquid

A becomes higher decreases
B becomes higher increases
C becomes lower decreases
D becomes lower increases

30 Which statements about water movement in plants are correct?

1 Water can pass through cell walls containing layers of cellulose fibres.

2 Water can pass through cell walls containing rings or spirals of lignin.

3 Water cannot pass through a band of suberin in cell walls.

A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

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31 The photomicrograph is a transverse section of a leaf.

3 2

Which labelled features are characteristic adaptations of many xerophytes?

A 1, 3, 4 and 5 B 1, 2 and 3 C 1, 2 and 5 D 2, 3, 4 and 5

32 A maize crop is growing successfully in a field in which the water potential of the soil is –40 kPa.

What is the most likely water potential of the cell sap in the root hair cell?

A –60 kPa B –40 kPa C –20 kPa D 0 kPa

33 The contraction of the heart is coordinated through electrical impulses passing through the cardiac
muscle.

What is the correct order of part of the sequence of these impulses?

A right and left atria → sinoatrial node → atrioventricular node → ventricular walls

B sinoatrial node → right and left atria → atrioventricular node → Purkyne tissue

C sinoatrial node → right and left atria → Purkyne tissue → atrioventricular node

D right and left atria → sinoatrial node → Purkyne tissue → ventricular walls

34 Which features allow the aorta to withstand high blood pressures?

A collagen fibres and elastin fibres

B collagen fibres and smooth muscle

C elastin fibres and endothelium

D endothelium and smooth muscle

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35 Which row is correct for the pulmonary artery?

blood carried thickness of wall lumen size

A oxygenated thin large
B oxygenated thick small
C deoxygenated thin large
D deoxygenated thick small

36 The graph shows oxygen dissociation curves of adult haemoglobin in different carbon dioxide
concentrations, 1 and 2.

100 1
2
80

percentage
60
saturation of
haemoglobin
with oxygen 40

20

0
0 2 4 6 8 10 12 14 16

partial pressure of oxygen / kPa

Which conditions could change the shape of curve 1 to the shape of curve 2?

A increased carbon dioxide concentration causing a decrease in pH

B increased carbon dioxide concentration causing an increase in pH

C decreased carbon dioxide concentration causing a decrease in pH

D decreased carbon dioxide concentration causing an increase in pH

37 What maintains the necessary concentration gradients for carbon dioxide and oxygen in the
lungs?

A good ventilation of the lungs

B large surface area of the alveoli

C the capillaries are close to the walls of the alveoli

D the walls of the alveoli are thin

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38 Which cells and tissues are present in the trachea?

cartilage ciliated epithelium

A

B
D C

goblet cells

39 The global mortality figures for some diseases in 2013 are shown in the table.

cause of death millions of

deaths
HIV / AIDS 1.50
TB 1.40
malaria 1.03
cholera 0.02

How many millions of people died in 2013 from the bacterial diseases listed in the table?

A 0.02 B 1.42 C 2.45 D 2.90

40 Why is it difficult for B-lymphocytes and T-lymphocytes to respond to the antigens on pathogens
that are intracellular parasites?

A The antigens are constantly mutating.

B The antigens can destroy the lymphocytes.

C The lymphocytes do not encounter the antigens.

D The lymphocytes do not recognise the antigens.

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BLANK PAGE

Copyright Acknowledgements:

Question 2 © B060/0031; MARILYN SCHALLER/SCIENCE PHOTO LIBRARY; TEM of the junction of two leaf cells; www.sciencephoto.com
Question 22 © P656/0023; BIOPHOTO ASSOCIATES/SCIENCE PHOTO LIBRARY; False-colour SEM of a group of human chromosomes;
www.sciencephoto.com
Question 31 © C011/5248; DR KEITH WHEELER/SCIENCE PHOTO LIBRARY; Oleander leaf, light micrograph; www.sciencephoto.com

Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every
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Cambridge Assessment International Education is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of the University of
Cambridge Local Examinations Syndicate (UCLES), which itself is a department of the University of Cambridge.

© UCLES 2019 9700/01/SP/22