ABC of Standardized Bioactive Ingredients

DIANA FOOD HEALTH & NUTRITION
Complete offer
© DIANA FOOD, Proprietary and confidential information

Part 1
H&N Expertise
© DIANA FOOD, Proprietary and confidential information 2

Our expertise
Agronomy & sourcing
• Over 40 years of agronomic know-how

• Screening and varietal selection to select the most
concentrated raw material in bioactives
• Dedicated agronomists in link with seed developers
and farmers
• Only non GMO raw materials
• Strict supplier’s assessment based on food safety
criteria
• Sustainable and local sourcing

Our expertise
Know-How
• Food grade processes

• Know-how in extraction and purification
• Dedicated factory to H&N extracts
• Organoleptic benefits preservation
• Quality control and traceability
• Organic certification
• GMP certification (NHP – Health Canada)

Our expertise
Technologies
Vacuum Drying Spray-Drying
Extraction
 Formulation
Concentration Blending
(water or Purification
hydroethanolic) Roller Drying

Our expertise
Regulatory and scientific support
• Possible bliographic reviews

• Support on the construction of health claim dossiers
• Support on regulatory status (GRAS, Pharma, Medical devices…)

Our expertise
Application
• Understanding of customer applications and constraints

• Formulation support, tableting pilot capacity
• Tailor-made solutions in accordance with legal compliance
• Diana’s expertise is used to develop customized product and support
customer’s request

Part 2
Standardized solutions

To answer precise market demands
Range characteristics & key advantages:
• Carefully selected raw material : sustainable, variety screening, full traceability,

authentication tests
• Food processes that preserve targeted bioactive and organoleptic properties
• Standardized in bioactives with defined analytical assay
• Fit market standards in term of specs
• Price competitiveness

ABC of standardized bioactive ingredients
Acai
Euterpe oleracea E&V WWB SP
Denomination Bioactive(s) Specification Carrier Reference
Acai juice powder Total polyphenols >6% (UV 280 nm) Maltodextrin SD00020001
Health positioning: superfruit, antioxidant, beauty, reduce oxidative stress, promote nitrite reduction to NO
EU claims USA claims

- Contains polyphenols (Min 250 mg polyphenols) - Naturally contains polyphenols (No min)

Acerola
Malpighia glabra L. E&V WWB SP HA
Denomination Bioactive Specification Carrier Reference
Organic acerola juice concentrate Natural vitamin C 17% ± 2 (HPLC) None CC00030002
Acerola juice concentrate Natural vitamin C 17% ± 2 (HPLC) None CC00030001
Organic acerola juice powder Natural vitamin C 17% ± 1 (HPLC) Org. maltodextrin SD00030001
Acerola juice powder Natural vitamin C 17% ± 1 (HPLC) Maltodextrin SD00030013
Acerola juice powder Natural vitamin C 34% ± 2 (HPLC) MgOH SD00030007

Acerola
Denomination Bioactive Specification Carrier Reference
Organic acerola juice powder Natural vitamin C ≥ 17% (HPLC) Org. maltodextrin SD00030028
Acerola juice powder Natural vitamin C ≥ 17% (HPLC) Maltodextrin SD00030029
Acerola juice powder Ratio 7.5:1 Maltodextrin SD00030008
Acerola preparation powder Vitamin C > 25% (HPLC) Maltodextrin SD00030011
Health positioning: support immunity and energy, support iron absorption, beauty (collagen metabolism, antioxidant),
superfruit, support memory and mood, support bone and joint health (collagen metabolism)

Acerola
EU claims
- Source of [natural] vitamin C: Min 12mg vit C per serving
- High in [natural] vitamin C : Min 24mg vit C per serving
EFSA health claims (min source of vit C product) :
- Vitamin C contributes to
• the normal function of the immune system
• the protection of cells from oxidative stress
• the reduction of tiredness and fatigue
• the regeneration of the reduced form of vitamin E
• normal collagen formation for the normal function of blood vessels, bones, cartilage, gums, skin and teeth
• normal energy-yielding metabolism
• normal functioning of the nervous system
• normal psychological function
- Vitamin C increases iron absorption
- Vitamin C contributes to maintain the normal function of the immune system during and after intense physical exercise (for 200mg vitamin C
daily intake)

Acerola
Helps fight free radicals in the body

USA claims Helps form collagen and connective tissue
- Good source of [natural] antioxidant vitamin C: Min 6 mg vit C Helps maintain healthy energy utilization
Helps protect against free radicals formed during exercise
/serving Helps reduce oxidative stress
- High in [natural] antioxidant vitamin C : Min 12 mg vit C /serving Immune support
Immune system support
- Documented Structure-function claims (min 6 mg vitC/serving): Important for healthy immune system function
Antioxidant protection
Important for proper collagen formation
Antioxidant support
Inhibits free radical damage to muscle cells during exercise
Antioxidants to help fight free radicals
Maintains a healthy immune system
Contributes to a normal function of the immune system
Oxidative stress support
Contributes to a normal functioning nervous system
Reduces oxidation due to exercise
Contributes to maintain the normal function of the immune system during and after intense
Supports a healthy energy metabolism
physical exercise
Supports antioxidant status
Contributes to normal energy metabolism
Supports healthy collagen formation
Contributes to the protection of cell constituents from oxidative damage
Supports healthy iron levels
Energy production
Supports normal collagen formation for healthy bones
Free radical defense
Supports normal collagen formation for healthy cartilage
Free radical protection
Supports normal collagen formation for healthy skin
Free radical scavenger
Supports normal collagen formation for healthy teeth and gums
Helps deflect free radicals produced during exercise
Supports normal energy metabolism

Apple Cider Vinegar
WWB HA
Apple cider vinegar powder Acetic acid 6-9% Maltodextrin SD01600001
Health positioning: glucose regulation (improve insulin sensitivity and lower blood glucose), weight loss aid (promote
satiety)

Aronia (or Chokeberry)
Aronia melanocarpa (Michx.) Elliot E&V WWB SP HA
Denomination Bioactive(s) Specification Reference
>15% (UV cyanidin chloride eq.) SD00090001

Aronia extract powder Anthocyanins
>20% (UV cyanidin-3-O glucoside chl. eq) SD00090002
Health positioning: superfruit, antioxidant, blood circulation and blood vessel protection, beauty, reduce oxidative
stress, anti-inflammatory
Suggested dosage (literature) : 50 mg anthocyanins

Artichoke
Cynara scolymus L. WWB HA
Artichoke leaf extract powder Cinnamic acids >2.5% (UV 325 nm) SD00100002
Artichoke leaf extract Cinnamic acids 2.5 - 3.5% (UV 325 nm) CC00100001 PHARMA
GRADE
Artichoke juice powder Cinnamic acids >2.5% (UV 325 nm) SD00110002
Artichoke juice concentrate Cinnamic acids >2% (UV 325 nm) CC00110001
Health positioning: Support liver and digestive functions (EU monograph for traditional herbal medicines THM),
cardiovascular health (cholesterol-lowering effect, LDL oxidation and atherosclerosis prevention, lower BP), renal water
excretion, satiety, glucose regulation, prebiotic
Suggested dosage for leaf extract (THM): 900-2400mg (in divided doses of 300-600mg)

Artichoke
Cynara scolymus L. WWB HA
Helps maintain vascular health

USA claims Helps reduce oxidative stress
- Documented Structure-Function claims: Helps support a healthy cardiovascular system
Antioxidant protection Helps support healthy circulation•
Antioxidant support Helps support vascular function
Antioxidants to help fight free radicals Liver and digestive support
Cholesterol support Maintains a healthy immune system
Digestive health Oxidative stress support
Digestive health support Promotes healthy bile production in the liver
Digestive support Promotes healthy circulation
Free radical defense Provides antioxidant support for the cardiovascular system
Free radical protection Supports antioxidant status
Free radical scavenger Supports blood vessel health
General wellness support Supports healthy aging
Healthy cardiovascular function Supports healthy bile flow
Healthy vascular function Supports healthy cholesterol metabolism
Helps fight free radicals in the body Supports healthy digestive function
Helps maintain cardiovascular health Supports heart health
Helps maintain healthy circulation Supports liver and cholesterol metabolism
Helps maintain normal function as we age Supports liver function
Helps maintain quality of life as we age

Berries’4Best
Grape, Acerola, Acai and Goji blend E&V WWB SP HA
Polyphenols 55±10% (UV 280 nm)

Blend of fruit extracts powder MD00920022
Natural vitamin C >5.5%
Health positioning: support immunity and energy, support iron absorption, beauty (collagen metabolism, antioxidant),
superfruits, cognitive functions, reduce oxidative stress, promote nitrite reduction to NO, support joint health (collagen
metabolism), cardiovascular prevention (resveratrol & anthocyanins from grape skin)
Suggested dosage : 450 mg
- High in vitamin C + all vitamin c health claims - Naturally high in vitamin C + EU claims on vit C as SFC

Bilberry
Vaccinium Myrtillus E&V WWB SP HA
Org. Wild Bilberry juice powder Maltodextrin SD01020001

Polyphenols >2.5% (UV 280nm catechin eq.)
Anthocyanins >0.5% (OD 510 nm cyan-3-gluco eq.)
Wilb Bilberry juice powder Maltodextrin SD01020006
> 25% (European Pharmacopoeia) Maltodextrin SD01020008
>1% (sepctro, delphinidin eq.) Glucose syrup SD01020009
Wild Bilberry extract powder Anthocyanins >10% (sepctro, delphinidin eq.) Glucose syrup SD01020010
>25% (European Pharmacopoeia) Glucose syrup SD01020011
>36% (European Pharmacopoeia) none SD01020012

Bilberry
Health positioning: ocular health (night vision), microcirculatory, vascular-related disorders (blood pressure, platelet
aggregation and cholesterol lowering, LDL oxidation prevention), antioxidant activity, superfruit
Suggested dosage : 50mg anthocyanins

EU claims
- Contains polyphenols (Min 250 mg polyphenols)

Bilberry

USA claims Helps protect against free radicals formed during exercise
- Naturally contains polyphenols (No min) Helps reduce oxidative stress
Helps support a healthy cardiovascular system
- Documented Structure-Function claims: Helps support healthy circulation
Helps support vascular function
Antioxidant support
Important for healthy immune system function
Eye and vision support
Overall brain health support
Promotes eye health
General wellness support
Promotes healthy circulation
Healthy eye function
Promotes visual health
Healthy eye support
Provides antioxidant support for the cardiovascular system
Helps maintain cardiovascular health
Supports blood vessel health
Helps maintain healthy circulation
Supports healthy aging
Helps maintain healthy heart function
Supports healthy vision and eye function
Helps maintain heart health
Supports heart health
Helps maintain normal function as we age
Supports vision
Vision support
Blackcurrant
Ribes nigrum L E&V WWB SP HA
Organic blackcurrant juice powder Polyphenols >2% (UV 280nm) SD00340001
Polyphenols >2% (UV 280nm)

Blackurrant powder SD00340005
Anthocyanins >0.35% (OD 510 nm cyan-3-gluco eq.)
Health positioning: antioxidant, blood circulation, traditionally used to decrease heavy legs sensation or hemorrhoid
troubles, healthy vision

Blueberry
Vaccinium angustifolium E&V WWB SP HA
>10% (Folin-Ciocalteu) SD02280001
Polyphenols >2% (Folin-Ciocalteu) SD02280003

Wild blueberry extract powder
Ratio 10:1 SD02280005
Wild blueberry juice powder Ratio 10:1 SD02280006

Blueberry
Health positioning: antioxidant, cognition support, oral health, reduce oxidative stress, promote nitrite reduction to
NO, cardiovascular protection
EU claims

Blueberry

USA claims Helps maintain memory
- Naturally contains polyphenols (No min) Helps maintain normal function as we age
- Documented Structure-Function claims: Helps maintain vascular health
Antioxidant support
Helps reduce oxidative stress
Helps support cognitive efficiency
Contributes to normal mental performance
Helps support cognitive health
Helps support endothelial function
Helps support healthy blood sugar levels already in the normal range
Helps support healthy circulation
Helps support healthy cognitive abilities
Help improve working memory
Helps support healthy cognitive skills
Helps boost memory
Helps support mental ability
Helps support mental clarity
Helps support mental function
Helps support mental sharpness
Helps support normal blood sugar balance
Helps to keep your mind sharp
Blueberry
Immune support Supports brain health and function

Immune system support Supports clarity, focus, memory
Important for healthy immune system function Supports cognition and brain health
Improves memory and learning and mental Supports cognitive performance
Inhibits free radical damage to muscle cells during exercise Supports focus, cognition, and memory
Maintains a healthy immune system Supports healthy aging
Maintains healthy cognitive function Supports healthy blood sugar
Maintenance of healthy brain function Supports healthy cognitive function
Overall brain health support Supports healthy glucose metabolism
Oxidative stress support Supports healthy insulin function
Promotes cognitive health and supports a healthy mind Supports healthy intellectual performance
Promotes normal cognitive function Supports heart health
Provides antioxidant support for the cardiovascular system Supports learning and memory
Reduces oxidation due to exercise Supports normal brain function
Support for optimal mental performance Supports normal cognitive function
Support memory and concentration Supports normal glucose metabolism
Supports a healthy mind
Supports brain function

Carrot
Daucus carota L. E&V WWB SP HA
Total carotenoids 50 000 ± 5 000 ppm (OD 450 nm)

Carrot extract HM00270002
Vit A (RE eq. / RAE eq.) 6875 ± 687 ppm / 3438 ± 343 ppm
Total carotenoids >400 ppm (OD 450 nm)

Carrot juice powder SD00280003
Vit A (RE eq. / RAE eq.) >55 ppm / >28 ppm
Total carotenoids >850 ppm (OD 450 nm)

Carrot juice concentrate CC00280009
Vit A (RE eq. / RAE eq.) >117 ppm / >58 ppm

Carrot
Health positioning: vitality (iron metabolism), immunity support, healthy vision, beauty (skin health), antioxidant

- Source of natural vitamin A: Min 120 µg vit A RE - Naturally source of antioxidant (min 500 IU)
- Vitamin A contributes to - Good source of natural vitamin A (min 500 IU)
• a normal function of the immune system - EFSA health claims as structure-function claims
• normal cell differentiation
• the maintenance of normal skin and mucus membranes
• the maintenance of normal vision
• normal iron metabolism

Cranberry
Vaccinium macrocarpon Aiton E&V WWB SP HA

Cranberry extract powder Total polyphenols ≥ 40% (Folin-Ciocalteu) VD00240008
15% PACs (BL-DMAC) /

Proanthocyanidins SD00240020
68% PACS Eur. Pharm
≥ 80% PACs VD00240001
≥ 50% PACs SD00240023

Cranberry extract powder
Proanthocyanidins (European
Pharmacopoeia) ≥ 40% PACs SD00240024
≥ 10% PACs SD00240028
≥ 7% PACs SD00240027

Cranberry

Organic cranberry extract powder Proanthocyanidins (Eur. Pharm) ≥ 10% PACs SD00240029
Proanthocyanidins (HPLC) ≥ 1% PACs SD00240025
≥ 5% PACs SD00240022
Cranberry extract powder Proanthocyanidins (BL-DMAC)
≥ 1% PACs SD00240026
Not Standardized NS SD00240032
Not Standardized NS SD00240034

Cranberry juice powder
SD00240004
Proanthocyanidins (Eur. Pharm) ≥ 1% PACs
SD00240036
Cranberry

Proanthocyanidins (Eur. Pharm) ≥ 7% PACs AD00240002
Cranberry powder
Proanthocyanidins (HPLC) ≥ 1.5% PACs AD00240001
Health positioning: urinary health, antioxidant, superfruit, beauty, reduce oxidative stress, promote nitrite reduction to
NO

Grape
Vitis vinifera E&V WWB SP HA
Polyphenols >90% (OD 280nm)

Grape skin extract powder Polyphenols >50% (Folin-Ciocalteu) SD01460002
Anthocyanins >4% (HPLC)
Health positioning: antioxidant, cardiovascular (blood circulation, and blood vessel protection), beauty, reduce
oxidative stress, anti-inflammatory
EU claims

Grape
Helps maintain normal function as we age

USA claims Helps maintain quality of life as we age
- Naturally contains polyphenols (No min) Helps maintain vascular health
- Documented Structure-Function claims: Helps reduce oxidative stress
Antioxidant support
Helps support cognitive efficiency
Helps support cognitive health
Helps support endothelial function
Helps support healthy circulation
Contributes to normal mental performance
Helps support healthy cognitive abilities
Helps support normal blood sugar balance
Immune support
Immune system support
Important for healthy immune system function
Maintenance of healthy brain function
Overall brain health support
Promotes cognitive health and supports a healthy mind

Grape
Provides antioxidant support for the cardiovascular system

Support for optimal mental performance
Supports a healthy mind
Supports brain function
Supports brain health
Supports brain health and function
Supports cognition and brain health
Supports focus, cognition, and memory
Supports healthy aging
Supports healthy blood sugar
Supports healthy cognitive function
Supports healthy intellectual performance
Supports heart health
Supports normal brain function
Supports normal cognitive function

Mannose
E&V WWB SP HA
D-Mannose powder Mannose (purity) 99 – 100% (internal method) MD00880002
Health positioning: prevention of urinary tract infections
Suggested dosage: 1000mg/D when taken alone, 500mg/D in combination with cranberry extract
- Can still be used as Medical device

Papaya
Carica papaya L. E&V WWB SP HA
Papaya powder ratio 3.5:1 SD01110001
Health positioning: digestion comfort (enzymatic activity), weight management

Pineapple
Ananas comosus L. E&V WWB SP HA
ratio 6.5:1 SD00070003
>1500 mUHB/g (1 UHb is

Pineapple juice powder
equivalent to 1 µmol of tyrosin /
Proteasic enzymatic activity RD00070005
minute liberated at 37°C in
standard conditions)
Health positioning: digestion comfort (enzymatic activity), weight management, support digestion for high protein diets

Prune
Prunus domestica L. E&V WWB SP HA
Prune powder on inulin Inulin soluble fiber >70% SD01430002
Health positioning: weight management (satiety effect of fibers), digestion (transit stimulation), prebiotic

Purple carrot
Polyphenols >2% (UV 280nm)

Organic purple carrot juice powder SD00310001
Anthocyanins >0.3% (0D 510nm)
Health positioning: Antioxidant, beauty, reduce oxidative stress, promote nitrite reduction to NO, blood circulation,
healthy vision

Spinach
Spinacia oleracea E&V SP
Spinach extract powder Polyphenols >1.5% (Folin-Ciocalteu) SD00690002
Health positioning: Antioxidant, supergreens, reduce oxidative stress, promote nitrite reduction to NO


Strawberry
Fragaria X ananassa E&V WWB SP
Strawberry extract powder Polyphenols >2% (Folin-Ciocalteu) SD00720019
>20% (Folin-Ciocalteu VD00720002
Health positioning: Antioxidant, beauty, reduce oxidative stress, promote nitrite reduction to NO


Tomato
Lycopersicum esculentum L. WWB SP HA
Tomato powder Carotenoids >1500 ppm (as lycopene eq.) SD01510003
Health positioning: Antioxidant, beauty (protection against cutaneous photodamage), bone health (reduce bone
resorption marker), cardiovascular protection (reduce platelet aggregation)
- Naturally contains lycopene (Min 830mg of powder) - Naturally contains lycopene (no min)

Part 3
Premiums

Premium Health Solutions
Innovation and science
Product Source Health Daily Description Studies Suggested claim
positionning dosis
Patented apple extract standardized 4 clinical Contributes to decrease

Glucose regulation 900 mg in Phloridzin. studies glucose absorption
Water extract of brocoli standardized In-vitro studies Helps to promote liver

Liver detoxification 100 mg in glucoraphanin detoxification
Natural source of nitric oxide Literature Helps improve muscle

Sports endurance 18 g precursors from red Beetroot review: 30+ efficiency
clinical studies
High bioavailability Complex of Clinical study Helps improve joint comfort

Joint Comfort and 1.5 g Collagen type II, Chondroitin sulfate, and mobility
Mobility hyaluronic acid.
Cardiovascular : 8.6 g Red beetroot juice powder or Literature Diets containing foods that
blood pressure concentrate standardized in review: 10+ are a good source of
reduction potassium and naturally high in NO clinical studies potassium and that are
precursors low in sodium may reduce
the risk of high blood
pressure and stroke**
**FDAMA approved health claim © DIANA FOOD, Proprietary and confidential information 45
Premium Health Solutions
Innovation and science
Health
Product Source Daily dosis Description Studies Suggested claim
positionning
Concentrated and purified Clinical
Helps maintain urinary
Urinary Tract Health 240 mg proanthocyanidins type-A from study on-
health
American cranberry. going
Proprietary blend of flavonols from

Canadian wild blueberry (V. Clinical Helps improve
Cognitive Health 600 mg
angustifolium) and grape study memory and focus
standardized to flavonols
Glucophenol™ is a mixture of
polyphenols from the Orleans Clinical Helps improve insulin
Glucose Regulation 277-554 mg
variety of strawberries and study sensitivity
cranberries.
Orophenol™ is a standardized
blend of polyphenols obtained from Support naturally oral
Oral Health 62.5 mg In-vitro study
Canadian wild blueberries, health
cranberries, and strawberries

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DIANA FOOD HEALTH & NUTRITION

© DIANA FOOD, Proprietary and confidential information

© DIANA FOOD, Proprietary and confidential information 2

• Over 40 years of agronomic know-how

© DIANA FOOD, Proprietary and confidential information 3

• Food grade processes

© DIANA FOOD, Proprietary and confidential information 4

Vacuum Drying Spray-Drying

© DIANA FOOD, Proprietary and confidential information 5

• Possible bliographic reviews

© DIANA FOOD, Proprietary and confidential information 6

• Understanding of customer applications and constraints

© DIANA FOOD, Proprietary and confidential information 7

© DIANA FOOD, Proprietary and confidential information 8

Range characteristics & key advantages:

• Carefully selected raw material : sustainable, variety screening, full traceability,

© DIANA FOOD, Proprietary and confidential information 9

Denomination Bioactive(s) Specification Carrier Reference

EU claims USA claims

© DIANA FOOD, Proprietary and confidential information 10

Denomination Bioactive Specification Carrier Reference

Acerola juice concentrate Natural vitamin C 17% ± 2 (HPLC) None CC00030001

Acerola juice powder Natural vitamin C 17% ± 1 (HPLC) Maltodextrin SD00030013

Acerola juice powder Natural vitamin C 34% ± 2 (HPLC) MgOH SD00030007

© DIANA FOOD, Proprietary and confidential information 11

Denomination Bioactive Specification Carrier Reference

Acerola juice powder Natural vitamin C ≥ 17% (HPLC) Maltodextrin SD00030029

Acerola juice powder Ratio 7.5:1 Maltodextrin SD00030008

Acerola preparation powder Vitamin C > 25% (HPLC) Maltodextrin SD00030011

© DIANA FOOD, Proprietary and confidential information 12

© DIANA FOOD, Proprietary and confidential information 13

Helps fight free radicals in the body

© DIANA FOOD, Proprietary and confidential information 14

Denomination Bioactive(s) Specification Carrier Reference

Apple cider vinegar powder Acetic acid 6-9% Maltodextrin SD01600001

© DIANA FOOD, Proprietary and confidential information 15

Denomination Bioactive(s) Specification Reference

>15% (UV cyanidin chloride eq.) SD00090001

© DIANA FOOD, Proprietary and confidential information 16

Denomination Bioactive(s) Specification Reference

© DIANA FOOD, Proprietary and confidential information 17

Helps maintain vascular health

© DIANA FOOD, Proprietary and confidential information 18

Denomination Bioactive(s) Specification Reference

Polyphenols 55±10% (UV 280 nm)

© DIANA FOOD, Proprietary and confidential information 19

Denomination Bioactive(s) Specification Carrier Reference

Org. Wild Bilberry juice powder Maltodextrin SD01020001

> 25% (European Pharmacopoeia) Maltodextrin SD01020008

>1% (sepctro, delphinidin eq.) Glucose syrup SD01020009

>25% (European Pharmacopoeia) Glucose syrup SD01020011

>36% (European Pharmacopoeia) none SD01020012

Suggested dosage : 50mg anthocyanins

© DIANA FOOD, Proprietary and confidential information 21

Helps maintain vascular health

Denomination Bioactive(s) Specification Reference

Organic blackcurrant juice powder Polyphenols >2% (UV 280nm) SD00340001

Polyphenols >2% (UV 280nm)

© DIANA FOOD, Proprietary and confidential information 23

Denomination Bioactive(s) Specification Reference

>10% (Folin-Ciocalteu) SD02280001

Polyphenols >2% (Folin-Ciocalteu) SD02280003

Ratio 10:1 SD02280005