88838001EN Manual 883 Basic IC Plus
88838001EN Manual 883 Basic IC Plus
2.883.0020
Manual
8.883.8001EN / 2019-12-12
Metrohm AG
CH-9100 Herisau
Switzerland
Phone +41 71 353 85 85
Fax +41 71 353 89 01
[email protected]
www.metrohm.com
Manual
8.883.8001EN / 2019-12-12
Technical Communication
Metrohm AG
CH-9100 Herisau
[email protected]
Table of contents
1 Introduction 1
1.1 Instrument description ......................................................... 1
1.2 Intended use ......................................................................... 2
1.3 Safety instructions ................................................................ 3
1.3.1 General notes on safety ........................................................... 3
1.3.2 Electrical safety ........................................................................ 3
1.3.3 Tubing and capillary connections ............................................. 4
1.3.4 Flammable solvents and chemicals ........................................... 4
1.3.5 Recycling and disposal ............................................................. 4
1.4 Symbols and conventions .................................................... 5
3 Installation 8
3.1 About this chapter ................................................................ 8
3.2 Initial installation .................................................................. 8
3.3 Installation diagram ........................................................... 11
3.4 Setting up the instrument .................................................. 14
3.4.1 Packaging .............................................................................. 14
3.4.2 Checks .................................................................................. 14
3.4.3 Location ................................................................................ 14
3.5 Capillary connections in the IC system ............................. 14
3.6 Installations on the rear of the instrument ...................... 17
3.6.1 Positioning and connecting the detector ................................ 17
3.6.2 Transport locking screws ........................................................ 18
3.6.3 Installing the drainage tubing ................................................ 19
3.7 Capillary and cable feed-throughs .................................... 21
3.8 Connecting the eluent bottle ............................................. 23
3.9 Installing the high-pressure pump .................................... 27
3.10 Installing an inline filter ..................................................... 27
3.11 Installing the pulsation absorber ...................................... 28
3.12 Injection valve ..................................................................... 29
3.13 Suppressor ........................................................................... 31
3.13.1 General information on the suppressor .................................. 31
3.13.2 Installing the suppressor ........................................................ 31
5 Troubleshooting 89
5.1 ............................................................................................. 89
6 Technical specifications 93
6.1 Reference conditions .......................................................... 93
6.2 Instrument ........................................................................... 93
6.3 Ambient conditions ............................................................ 93
6.4 Housing ............................................................................... 94
6.5 Injection valve ..................................................................... 94
6.6 Suppressor ........................................................................... 94
6.7 Peristaltic pump .................................................................. 95
6.8 Detector ............................................................................... 95
6.9 Power connection ............................................................... 95
6.10 Interfaces ............................................................................. 95
6.11 Weight ................................................................................. 96
7 Accessories 97
Index 98
Table of figures
Figure 1 883 Basic IC plus front ....................................................................... 6
Figure 2 883 Basic IC plus rear ........................................................................ 7
Figure 3 Installation diagram 883 Basic IC plus .............................................. 12
Figure 4 Positioning the detector .................................................................. 17
Figure 5 Drainage tubing .............................................................................. 20
Figure 6 Capillary feed-throughs on the door ................................................ 22
Figure 7 Installing the eluent bottle cap ........................................................ 23
Figure 8 Installing tubing weighting and aspiration filter ................................ 25
Figure 9 High-pressure pump with purge valve .............................................. 27
Figure 10 Inline filter ....................................................................................... 28
Figure 11 Pulsation absorber ........................................................................... 28
Figure 12 Exchanging the sample loop ............................................................ 30
Figure 13 Suppressor – Connection capillaries ................................................. 34
Figure 14 Peristaltic pump ............................................................................... 42
Figure 15 Front of conductivity detector ......................................................... 43
Figure 16 Rear of conductivity detector ........................................................... 44
Figure 17 Connection detector–MSM ............................................................. 45
Figure 18 High-pressure pump – Parts ............................................................. 60
Figure 19 High-pressure pump – Cross-section ................................................ 67
Figure 20 Tool for piston seal (6.2617.010) ..................................................... 67
Figure 21 Removing the piston cartridge from the pump head ........................ 68
Figure 22 Inserting the piston seal into the tool ............................................... 69
Figure 23 Parts of the piston cartridge ............................................................ 70
Figure 24 Inline filter – Removing the filter ...................................................... 74
Figure 25 Parts of the suppressor .................................................................... 77
Figure 26 Pump tubing connection – Replacing the filter ................................. 85
1 Introduction
High-pressure pump
The intelligent and low-pulsation high-pressure pump pumps the eluent
through the IC system. It is equipped with a chip where its technical speci-
fications and "life history" (operating hours, service data, etc.) are stored.
Inline filter
Inline filters protect the separation column reliably from potential contami-
nation from the eluent. The filter pads with 2 µm pore size can be
replaced quickly and easily. They remove particles from the solutions, such
as bacteria and algae.
Pulsation absorber
The pulsation absorber protects the separation column from damage
caused by pressure fluctuations, e.g. when the injection valve is switched,
and reduces interfering pulsations during highly sensitive measurements.
Injection valve
The injection valve connects the eluent path to the sample path. By a
quick and precise switching of the valve, a quantity of sample solution
defined by the size of the sample loop is injected and flushed to the sepa-
ration column with the eluent.
Suppressor
The suppressor consists of the suppressor drive, the MSM Rotor A and an
adapter.
Peristaltic pump
The peristaltic pump is used for pumping sample and auxiliary solutions. It
can rotate in both directions.
Conductivity detector
The conductivity detector continuously measures the conductivity of the
liquid passing through and outputs these signals in digital form (DSP –
Digital Signal Processing). The conductivity detector exhibits outstanding
thermal stability and thus guarantees reproducible measuring conditions.
Separation column
The intelligent separation column separates different components accord-
ing to their interactions with the column. Metrohm separation columns
are equipped with a chip where their technical specifications and history
(start-up, operating hours, injections etc) are stored.
WARNING
WARNING
WARNING
Supply voltage
WARNING
WARNING
CAUTION
Leaks in tubing and capillary connections are a safety risk. Tighten all
connections well by hand. Avoid applying excessive force to tubing con-
nections. Damaged tubing ends lead to leakage. Appropriate tools can
be used to loosen connections.
Check the connections regularly for leakage. If the instrument is used
mainly in unattended operation, then weekly inspections are manda-
tory.
WARNING
More details about the disposal of your old instrument can be obtained
from your local authorities, from waste disposal companies or from your
local dealer.
2.1 Front
3 7
8
4
9 Purge valve
2.2 Rear
3 Installation
NOTICE
8 Initial start-up
(see chapter 3.18, page 47)
■ Switch on the PC and start MagIC Net.
■ Switch on the instrument.
■ Deaerate the high-pressure pump.
■ Adjust the contact pressure of the peristaltic pump.
■ Rinse the instrument without column(s).
15
6
15
5 18 7
4
15
15 16
8 18
15 15
9
17
10 15
3
11 20 19
12
14 2
13
3.4.2 Checks
Immediately after receipt, check whether the shipment has arrived com-
plete and without damage by comparing it with the delivery note.
3.4.3 Location
The instrument has been developed for operation indoors and may not be
used in explosive environments.
Place the instrument in a location of the laboratory which is suitable for
operation and free of vibrations and which provides protection against
corrosive atmosphere and contamination by chemicals.
The instrument should be protected against excessive temperature fluctua-
tions and direct sunlight.
Pressure screws
Three types of pressure screws are used in the IC system:
Pressure screws are tightened and loosened by hand. A tool is not
needed.
Also see: PEEK pressure screws 1x1 video on the Internet http://ic-
help.metrohm.com.
Connection capillaries
PEEK capillaries and PTFE capillaries are used in the IC system.
PEEK capillaries (poly- PEEK capillaries are pressure-stable up to 400 bar (depending on the inner
etheretherketone) diameter), flexible, chemically inert and have an extremely smooth surface.
They can be readily cut down to the desired length with the capillary cut-
ter (6.2621.080).
Use:
PTFE capillaries PTFE capillaries are transparent and enable visual tracing of the liquids to
(poly(tetrafluoroethy- be pumped. They are chemically inert, flexible and temperature-resistant
lene)) up to 80 °C. They can be readily cut down to the desired length with the
capillary cutter (6.2621.080).
Use:
PTFE capillaries (6.1803.0x0) are used for the low-pressure section.
■ PTFE capillaries with an inner diameter of 0.5 mm for sample process-
ing and for the transfer of rinsing solutions (they are not necessarily
included in the scope of delivery of the instrument).
Capillary connections
NOTICE
1 Wipe off the end of the capillary with a cloth dampened with ace-
tone.
2 Slide the pressure screw over the capillary. Ensure that the capillary
protrudes 1 to 2 mm from the tip of the pressure screw.
4 Only then start turning the pressure screw. Hold the capillary in the
stop position while turning it shut.
NOTICE
5
5
5 Capillary feed-throughs
For feeding through the capillaries out of the
detector chamber.
CAUTION
1 Remove all of the transport locking screws with the hex key.
Store the transport locking screws in a safe place. Reinsert the transport
locking screws each time you transport the instrument a significant dis-
tance.
WARNING
3
4
3 Connect the drainage tubing (5-2) and the drainage tubing (5-4) to
each other using the Y connector (5-5).
Do not feed capillaries through the Luer connectors (6-1). The capillaries
are fastened with PEEK pressure screws (6-3) from inside to the Luer con-
nector. From outside, liquid can be aspirated or injected with a syringe.
■ Start by pushing the tubing weighting onto the loose end of the
eluent aspiration tubing.
■ Then push the clamping screw onto the loose end of the eluent
aspiration tubing.
■ Lastly, push the filter holder onto the loose end of the eluent aspi-
ration tubing and screw it onto the tubing nipple.
The end of the tubing should extend approximately 1 cm.
NOTICE
In order to avoid air bubbles after the installation of the aspiration fil-
ter, we recommend pre-rinsing the aspiration filter with water or elu-
ent.
For pre-rinsing, you will need the holder for the eluent aspiration fil-
ter (6.2744.360), a syringe and the purge needle (6.2816.040).
This procedure can also be found in the video "Inserting a new aspi-
ration filter" at the following link: ic-help.metrohm.com
NOTICE
■ Place the loose end of the eluent aspiration tubing into the aspira-
tion filter.
The end of the tubing should reach approximately to the center of
the aspiration filter.
■ Tighten the aspiration filter to the filter holder.
NOTICE
■ Remove the plastic cover from the large opening of the adsorber
tube. Fill the adsorber tube and close it again using the plastic
cover.
■ Insert the adsorber tube into the bottle cap's large opening. Fas-
ten it to the bottle cap using the SGJ clip (6.2023.020).
6.2744.070
6.2744.070
6.2821.120
1
4
3 1
NOTICE
3 Pressure screw
Fastened to Port 3.
NOTICE
3.13 Suppressor
3.13.1 General information on the suppressor
The suppressor is used for chemical suppression during anion analysis. It is
pressure-stable, robust and resistant to solvents. It consists of 3 suppressor
units, which are used for suppression, regenerated with sulfuric acid and
rinsed with ultrapure water in rotation.
Analyzing special environmental samples may require using phosphoric
acid for regeneration.
You need the adapter (6.2842.020) in order to insert the rotor into the
suppressor drive.
CAUTION
■ Clean the sealing surface of the rotor with ethanol using a lint-
free cloth.
■ Insert the rotor into the adapter so that the tubing connections
on the rear of the rotor fit into the corresponding recesses inside
the adapter and one of the 3 holes of the rotor is visible in the
slot of the adapter.
NOTICE
1 out 2 in
Outlet capillary for the eluent. Inlet capillary for the eluent.
Recommended installation
Alternative installation
2 Connect the capillary labeled out to the detector inlet capillary using
one coupling (6.2744.040) and two pressure screws (6.2744.070)
(see manual of the detector).
1 Connect the detector outlet capillary and the capillary labeled rinsing
solution to each other using a coupling (6.2744.040) and two pres-
sure screws (6.2744.070).
3 Connect the PTFE capillary from the rinsing solution bottle to the inlet
of the pump tubing.
6.1826.310 Pump tubing LFL (orange/ PVC (Tygon®) 0.38 mm Pump tubing for bromate deter-
green), 3 stoppers mination using the triiodide
method.
6.1826.320 Pump tubing LFL (orange/ PVC (Tygon®) 0.48 mm For acceptor solutions for Inline
yellow), 3 stoppers Dialysis and for Inline Ultrafiltra-
tion.
6.1826.330 Pump tubing LFL (orange/ PVC (Tygon®) 0.64 mm No special applications.
white), 3 stoppers
6.1826.340 Pump tubing LFL (black/ PVC (Tygon®) 0.76 mm For sample solution in Inline Dial-
black), 3 stoppers ysis.
6.1826.360 Pump tubing LFL (white/ PVC (Tygon®) 1.02 mm For sample transfer.
white), 3 stoppers
6.1826.380 Pump tubing LFL (gray/ PVC (Tygon®) 1.25 mm For Inline Dilution.
gray), 3 stoppers
6.1826.390 Pump tubing LFL (yellow/ PVC (Tygon®) 1.37 mm For sample solution in Inline
yellow), 3 stoppers Ultrafiltration.
1 Select pump tubing suitable for the application (see table 2, page
38).
2 Select an adapter suitable for the pump tubing. The adapters are
included with the pump tubing connection with locking nut and filter
(6.2744.180).
6.1826.310 (orange/green)
6.1826.320 (orange/yellow)
6.1826.330 (orange/white)
6.1826.340 (black/black)
6.1826.360 (white/white)
6.1826.380 (gray/gray)
6.1826.390 (yellow/yellow)
6.1826.420 (orange/yellow)
NOTICE
Pieces of pump tubing are consumables. The service life of the pump
tubing depends on the contact pressure, among other factors.
1 ■ Fully loosen the contact pressure lever , i.e. press it all the way
down.
■ In the software, activate the drive of the peristaltic pump with the
desired speed.
■ Raise the contact pressure lever one step at a time until liquid
flows.
7 Mounting bolt
1
3
3 Type plate
With serial number.
NOTICE
1 3 2
4
Figure 17 Connection detector–MSM
NOTICE
1 Insert the USB cable into the computer connection socket on the rear
of the instrument labeled PC.
WARNING
NOTICE
CAUTION
The separation column and the guard column are not permitted to be
installed at the time of the initial start-up.
Make sure that a coupling (6.2744.040) is being used instead of the
columns.
3 Starting equilibration
■ Start the equilibration in MagIC Net: Workplace ▶ Run ▶ Equi-
libration ▶ Start HW.
■ Push the end of the purge needle (6.2816.040) over the end of
the purge capillary on the purge valve.
■ Insert the syringe (6.2816.020) in the Luer connector of the purge
needle.
■ Open the purge valve using the rotary knob (approx. ½ turn).
■ Switch on the high-pressure pump in MagIC Net.
■ Use the syringe to aspirate eluent until there are no more air bub-
bles in the eluent aspiration tubing.
■ Switch off the high-pressure pump in MagIC Net.
■ Seal the purge valve using the rotary knob.
■ Remove the syringe from the purge needle.
■ Pull the purge needle out of the purge capillary.
NOTICE
NOTICE
CAUTION
New guard columns are filled with a solution and sealed with stoppers
or caps on both sides.
Before inserting the guard column, ensure that this solution can be
mixed with the eluent being used (follow the information provided by
the manufacturer).
NOTICE
The guard column may not be connected until after the instrument has
already been put into operation once (see chapter 3.18, page 47). The
guard column and the separation column have to be replaced by a cou-
pling (6.2744.040) until then.
CAUTION
■ Fasten the inlet of the guard column to the column inlet capillary
using a short pressure screw (6.2744.070).
■ If the guard column is connected to the separation column using
a connection capillary, fasten this connection capillary to the
guard column outlet with a pressure screw.
NOTICE
CAUTION
New separation columns are filled with a solution and sealed with stop-
pers on both sides. Before inserting the column, ensure that this solu-
tion can be mixed with the eluent being used (follow the information
provided by the manufacturer).
NOTICE
Connect the separation column only after the initial start-up of the
instrument. Until that point, insert a coupling (6.2744.040) instead of
the guard column and separation column.
CAUTION
3.21 Conditioning
In the following cases, the system must be conditioned with eluent until a
stable baseline has been reached:
■ After installation
■ After each time the instrument is switched on
■ After each eluent change
NOTICE
CAUTION
Ensure that the configured flow rate is not higher than the flow
rate permitted for the corresponding column (refer to the column
leaflet and chip data record).
3 Starting equilibration
■ Start the equilibration in MagIC Net: Workplace ▶ Run ▶ Equi-
libration ▶ Start HW.
■ Visually inspect whether all capillaries and their connections from
the high-pressure pump to the detector are leak-tight. If eluent is
leaking out anywhere, tighten the corresponding pressure screw
further, or loosen the pressure screw, check the end of the capil-
lary and shorten it using the capillary cutter if necessary and
retighten the pressure screw.
4.1 IC system
4.1.1 Operation
In order to avoid disruptive temperature influences, protect the analysis
system, including the eluent bottle, from direct sunlight.
4.1.2 Care
The instrument requires appropriate care. Excess contamination of the
instrument results in malfunctions and a reduction in the service life of the
sturdy mechanical and electronic components.
The instrument must be cleaned immediately if chemicals or solvents are
spilled on it. In particular, the plug connections (particularly the power
plug) must be protected against contamination.
CAUTION
WARNING
1 In the software, stop the hardware and wait until the pressure in the
high-pressure pump has been released.
2 Remove the guard column and the separation column from the elu-
ent path. Connect the connection capillaries directly with each other
using a coupling (6.2744.040).
5 Rinse the pump tubing of the peristaltic pump used for pumping the
regeneration solution with water for 15 minutes at level 1. Finish by
releasing the contact pressure at the peristaltic pump.
3 Remove the coupling and install the guard column and the separa-
tion column (see Chapter 3.19, page 49 and Chapter 3.20, page 51).
4.3 Door
CAUTION
NOTICE
To change the eluent, remove the guard column and the separation col-
umn. Connect the capillaries using a coupling (6.2744.040) and two
pressure screws (6.2744.070).
CAUTION
■ Ensure that no precipitates can form when changing the eluent. Salt
crystals between the piston and seal cause abrasive particles that can
find their way into the eluent. These particles lead to contaminated
valves, an increase in pressure and, in extreme cases, scratched pistons
and thus to leakages in the high-pressure pump.
Immediately successive solutions must always be miscible. If the system
must be rinsed with organic solvents, use several solvents with rising or
falling lipophilicity.
■ In order to protect the pump seals, ensure that the pump is never oper-
ated dry. Therefore ensure that the eluent supply is correctly connected
and that there is enough eluent in the eluent bottle each time before
turning on the pump.
NOTICE
You can find a video sequence for this task in the Multimedia Guide IC
Maintenance or on the Internet at http://ic-help.metrohm.com/.
Maintenance interval The following parts of the high-pressure pump must be serviced at least
once per year:
■ Inlet valve (6.2824.170)
■ Outlet valve (6.2824.160)
■ Piston seal (6.2741.020)
■ Zirconium oxide piston (6.2824.070)
Maintenance tasks can also be carried out if the following problems occur:
■ Unstable baseline (pulsations, flow fluctuations)
CAUTION
Recommended pro- We recommend the following procedure for the maintenance of the
cedure pump head:
1. Service the inlet valve and the outlet valve.
2. Remove the pump head.
3. Service both pistons, one after the other.
a. Remove piston.
b. Dismantle the piston.
c. Replace the piston seal.
d. Replace the zirconium oxide piston.
e. Assemble the piston.
f. Insert the piston.
4. Reinstall the pump head.
You can find brief video sequences on the following maintenance steps on
the Internet at http://ic-help.metrohm.com/.
Spare parts If the outlet valve cannot be cleaned, you will need a new outlet valve
(6.2824.160) for this step.
■ Insert the outlet valve into the outlet valve holder (the seal must
be visible) (1).
■ Screw the outlet valve holder up into the pump head (2) and
tighten it firmly by hand and then retighten it one additional ¼
turn using the adjustable wrench (3).
■ Tighten the connection capillary to the auxiliary piston back onto
the outlet valve holder.
Spare parts If the inlet valve cannot be cleaned, you will need a new inlet valve
(6.2824.170) for this step.
■ Insert the inlet valve into the inlet valve holder (the seal must be
visible) (1).
■ Screw the inlet valve holder up into the pump head (2) and
tighten it firmly by hand and then retighten it one additional ¼
turn using the adjustable wrench (3).
■ Refasten the connection capillary to the connection of the eluent
aspiration tubing (18-7).
Prerequisites:
■ Is the high-pressure pump switched off?
■ Has the pressure been released?
■ Is the instrument switched off?
1 Release the coupling from the pressure screw and seal it with a stop-
per.
2 Unscrew and remove the pressure screw on the pump head's outlet
(18-8).
3 Loosen and remove the four fastening screws (18-3) using the hex
key.
9 Piston cartridge
1 Tip 2 Sleeve
Spare part For this step, you need a new piston seal (6.2741.020).
CAUTION
Screwing the (6.2617.010) special tool for the piston seal into the
piston seal destroys this completely!
CAUTION
Avoid touching the sealing surface in the pump head with the
tool!
Only screw the tip (20-1) of the tool for the piston seal far enough
into the piston seal that the seal can be pulled out.
Prerequisites:
■ The pump head has been removed (see "Removing the pump head",
page 65).
■ The piston cartridge is removed (see "Replacing the piston seal", page
68).
For this task, you need the following accessories:
■ Zirconium oxide piston (6.2824.070)
CAUTION
Inside the piston cartridge there is a taut spring that can launch
out of the piston cartridge if the tension is released suddenly.
When opening the piston cartridge, counteract the pressure from
the spring and carefully unscrew the cartridge.
■ Rinse the other parts of the piston with ultrapure water and dry
with a lint-free cloth.
NOTICE
The pump head cannot be mounted the wrong way, as it has fastening
bolts with different bore hole depths, i.e., one fastening bolt is longer
than all others. The bore hole with the greatest depth must therefore
be aligned with the longest bolt.
1 ■ Push the pump head onto the four fastening bolts (1).
■ Tighten the four fastening screws using the hex key (6.2621.030)
alternating crosswise.
NOTICE
You can find a video sequence for this task in the Multimedia Guide IC
Maintenance or on the Internet at http://ic-help.metrohm.com/.
Maintenance interval The filter must be replaced at least every 3 months; it may need to be
replaced more frequently, depending on the application.
3 Filter (6.2821.130)
Pack contains 10 pieces.
CAUTION
4.10 Suppressor
4.10.1 Notes for operating the suppressor
To protect the suppressor against foreign particles or bacterial growth, a
pump tubing connection with filter (6.2744.180) (see "Installing the pump
tubing", page 39) must be mounted between the peristaltic pump (see
chapter 3.14.2, page 42) and the inlet capillaries of the suppressor.
NOTICE
The suppressor units must never be regenerated in the same flow direc-
tion the eluent is pumped. Therefore, always mount the inlet and outlet
capillaries according to the diagram outlined in chapter Connecting the
suppressor, page 33.
The suppressor consists of 3 suppressor units, which are used for suppres-
sion, regenerated with regeneration solution and rinsed with ultrapure
water in rotation. In order to record every new chromatogram under com-
parable conditions, you should normally work with a freshly regenerated
suppressor.
CAUTION
CAUTION
CAUTION
NOTICE
CAUTION
Pump tubing made of PVC must not be used for solutions containing
organic solvents.
We recommend using the high-pressure pump for regeneration.
CAUTION
■ Insert the rotor (25-3) into the adapter (see "Inserting the MSM
rotor into the adapter", page 31).
■ Insert the adapter into the suppressor drive (see "Inserting the
adapter into the suppressor drive", page 32).
The rotor's sealing surface is located approx. 4 mm deep inside
the suppressor drive if the adapter with the rotor is inserted cor-
rectly. If this is not the case, the adapter must be moved into the
correct position from below by means of a pointed object.
■ Insert the connecting piece into the suppressor drive so that con-
nector 1 is on top and the 3 pins of the connecting piece fit into
the corresponding recesses on the suppressor drive.
■ Reattach the union nut (25-1) and tighten by hand (do not use a
tool).
CAUTION
■ Insert the rotor (25-3) into the adapter (see "Inserting the MSM
rotor into the adapter", page 31).
■ Insert the adapter into the suppressor drive (see "Inserting the
adapter into the suppressor drive", page 32).
The rotor's sealing surface is located approx. 4 mm deep inside
the suppressor drive if the adapter with the rotor is inserted cor-
rectly. If this is not the case, the adapter must be moved into the
correct position from below by means of a pointed object.
■ Insert the connecting piece into the suppressor drive so that con-
nector 1 is on top and the 3 pins of the connecting piece fit into
the corresponding recesses on the suppressor drive.
■ Reattach the union nut (25-1) and tighten it by hand.
CAUTION
The service life of the pump tubing primarily depends on the contact
pressure.
If the peristaltic pump is switched off for long periods, lift up the tubing
cartridges on the right side by releasing the snap-action levers. This
ensures that the contact pressure will be maintained once it has been
set.
CAUTION
3 Filter screw
You can determine if the applied rinsing time is sufficient by measuring the
sample carry-over directly. Proceed as follows to do this:
NOTICE
5 Troubleshooting
Marked drop in Leak in the system. Check the capillary connections and seal leaks,
pressure. if necessary (see chapter 3.5, page 14).
The baseline has a The eluent path has a leak. Check the eluent path and fix the leak.
large amount of
If necessary, tighten the pressure screws using
noise.
a wrench (6.2739.000).
High-pressure pump – Con- Clean the pump valves (see chapter 4.6, page
taminated pump valves. 60).
The eluent path is blocked. Check the eluent path and eliminate the block-
age.
Contaminations in the elu- Check the quality of the chemicals and the
ent water.
High-pressure pump – Replace the piston seals (see chapter 4.6, page
Defective piston seals. 60).
The baseline is drift- Leak in the system. Check all capillary connections and seal leaks,
ing. if necessary (see chapter 3.5, page 14).
The organic solvent in the ■ Check the eluent bottle cap (see chapter
eluent is evaporating. 3.8, page 23).
■ Constantly stir the eluent.
The pressure in the The inline filter Replace the filter (6.2821.130) .
system markedly (6.2821.120) is blocked.
increases.
The suppressor is blocked. ■ Regenerate the suppressor .
Guard column – Blocked. Replace the guard column (see chapter 3.19,
page 49).
Injection valve – blocked. Have the valve cleaned (by a Metrohm service
engineer).
The retention times Eluent - Incorrect concen- Create eluent with correct concentration.
in the chromato- tration
gram have changed
Separation column – ■ Regenerate the separation column (see
unexpectedly.
Diminished separating effi- chapter 4.14.4, page 88).
ciency. ■ Replace the separation column (see "Con-
necting the separation column", page 53).
The eluent contains gas ■ Deaerate the high-pressure pump (see fig-
bubbles. ure , page 48).
Peak areas are lower Sample – There is a leak in Find and fix the leak in the sample path.
than expected. the sample path.
Sample – The sample path Find and eliminate the blockage in the sample
is blocked. path.
Sample – The sample loop Adjust the transfer time for the sample (see
is not (completely) filled. "Determining the transfer time", page 86).
The peristaltic pump Peristaltic pump – Contact Correctly set the contact pressure (see "Setting
is pumping too little. pressure too weak. the contact pressure correctly", page 41).
Peristaltic pump – Filter Replace the filter (see "Replacing the filter",
blocked. page 85).
Data of the separa- Column chip dirty. Clean the contact surfaces of the column chip
tion column cannot with ethanol.
be read.
Column chip is defective. 1. Save column configuration in MagIC Net.
2. Notify Metrohm Service.
Individual peaks are Sample – Sample carry- Check the rinsing time (see "Checking the rins-
greater than over from previous meas- ing time", page 87).
expected. urements.
The retention times The eluent path has a leak. Check all of the connections along the eluent
are poorly reprodu- path and fix the leak.
cible.
The eluent path is blocked. Check the eluent path and eliminate the block-
age.
Extreme spread of Capillary connections – Check the capillary connections (see chapter
the peaks in the Dead volume in the sys- 3.5, page 14) (use PEEK capillaries with an
chromatogram. tem. inner diameter of 0.25 mm between the injec-
Splitting (dual tion valve and detector).
peaks)
Guard column – Dimin- Replace the guard column (see chapter 3.19,
ished efficiency. page 49).
Precision problems - Injection valve – Sample Check the installation of the sample loop (see
the measured values loop. "Optional: Exchanging the sample loop",
are highly scattered. page 29).
6 Technical specifications
6.2 Instrument
IC system ■ Metal-free IC system
■ Compact system with modular design
Material Painted polyurethane hard foam without CFCs, fire class V0
Operating pres- ■ 0 - 50 MPa (500 bar) high pressure pump
sure range ■ 0 - 35 MPa (350 bar) standard-PEEK system
Intelligent com- iPump, iDetector, iColumn, MagIC Net
ponents
Storage
Ambient tem- –20 - +70 °C
perature
Transport
Ambient tem- –40 - +70 °C
perature
6.4 Housing
Dimensions
Width 262 mm
Height 468 mm
Depth 362 mm
Base tray, housing Polyurethane hard foam (PUR) with flame retardation for fire class
and cover plate UL94V0, CFC-free, coated
material
Controls
Indicators LED standby indicator
On/off switch On the rear of the instrument
6.6 Suppressor
Resistance to sol- No restriction
vents
Switching time Typ. 100 ms
Operating pres- 2.5 MPa (25 bar), valve function prevents damage at overpressure
sure
6.8 Detector
You can find the technical specifications for the detector in the manual for
the detector.
6.10 Interfaces
USB
Entry 1 USB upstream, type B (for connection to the PC)
6.11 Weight
Weight 14.8 kg (without accessories)
7 Accessories
Up-to-date information on the scope of delivery and optional accessories
for your product can be found on the Internet. You can download this
information using the article number as follows:
2 Enter the article number (e.g. 2.883.0020) into the search field.
The search result is displayed.
NOTICE
Index
A D Initial installation ........................ 8
Ambient conditions .................. 93 Detector Injection valve ............................ 1
Anion suppressor Cable connection ............... 17 Installation ......................... 29
Regeneration ...................... 78 Conductivity detector ......... 43 Maintenance ...................... 76
Aspiration filter (6.2821.090) .... 58 Interface ............................. 95 Technical specifications ....... 94
Aspiration tubing for eluent ...... 23 Position .............................. 17 Inline filter ................................ 27
Dimensions .............................. 94 Installation
B Door ........................................ 58 Conductivity detector ......... 43
Baseline Drainage tubing Connections ....................... 14
Condition ........................... 55 Installation ......................... 19 Drainage tubing ................. 19
Unstable ............................. 61 Eluent bottle ....................... 23
E Guard column .................... 49
C Electrostatic charge .................... 4 Injection valve .................... 29
Cable feed-throughs ................. 21 Eluent Peristaltic pump .................. 38
Capillaries Aspirate .............................. 23 Pulsation absorber .............. 28
Installation ......................... 14 Change .............................. 59 Pump tubing ...................... 38
Capillary feed-throughs ............. 21 Manufacturing ................... 58 Separation column ............. 51
Carry-over ................................ 86 Eluent bottle Suppressor ......................... 31
Clean Installation ......................... 23 Interface
Inlet valve ........................... 64 Equilibration ....................... 48, 55 USB .................................... 95
Outlet valve ........................ 62 Interfaces ................................. 95
Suppressor ......................... 80 F
Column Feed-throughs L
see "Separation column" .... 51 Capillaries ........................... 21 Leak-tightness .......................... 48
Column recognition .................. 95 Filter Leakage ................................... 61
Computer connection ............... 45 Aspiration filter ................... 58 Leaky piston seals ..................... 61
Conditioning ............................ 55 see "Inline filter" ................. 27 Loop
Conductivity detector Flow fluctuations ...................... 61 See also "Sample loop" ....... 29
Cable connection ............... 17 Frequency ................................ 95
Capillary connection ........... 43 Front .......................................... 6 M
Position .............................. 17 Front of the instrument .............. 6 Maintenance
Connect High-pressure pump ........... 60
Power grid .......................... 46 G Injection valve .................... 76
To computer ....................... 45 Guard column Peristaltic pump .................. 84
Connection Installation ......................... 49 Suppressor ......................... 76
Mains ................................. 95 Rinse .................................. 50 Material .................................... 94
Connections
Installation ......................... 14 H O
Contamination Heavy metals Operation
High-pressure pump ........... 60 Contamination of suppressor Peristaltic pump .................. 84
Valves for the high-pressure ........................................... 78 Suppressor ......................... 76
pump ................................. 61 High-pressure pump Organic contamination
Contamination of suppressor Maintenance ...................... 60 Suppressor ......................... 78
Heavy metals ...................... 78 Protection .................... 19, 59 Overview of the instrument ........ 6
Organic ............................. 78 Housing ................................... 94
Crystallization Humidity .................................. 93 P
High-pressure pump ........... 60 Peristaltic pump
I Installation ......................... 38
IC column Maintenance ...................... 84
see "Separation column" .... 51 Mode of operation ............. 42