Prostate Cancer Screening With Prostate-Specific Antigen (PSA) Test A Systematic Review and Meta-Analysis
Prostate Cancer Screening With Prostate-Specific Antigen (PSA) Test A Systematic Review and Meta-Analysis
Minnesota, USA
6Department of Health Research Methods, Evidence, and Impact, McMaster University, Hamilton, ON, Canada
7Velindre NHS Trust, Cardiff University Library Services, Velindre Cancer Centre, Cardiff, Wales
8Division of General Internal Medicine and Division of Epidemiology, Department of Internal medicine,
Copyright Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under
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Abstract
Objective
To investigate the efficacy and safety of prostate-specific antigen (PSA) testing to screen for prostate
cancer.
Design
Systematic review and meta-analysis.
Data sources
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Electronic search of Cochrane Central Register of Controlled Trials, Web of Science, Embase, Scopus,
OpenGrey, LILACS, and Medline, and search of scientific meeting abstracts and trial registers to April
2018.
Data extraction
At least two reviewers screened studies, extracted data, and assessed the quality of eligible studies. A
parallel guideline committee (BMJ Rapid Recommendation) provided input on the design and
interpretation of the systematic review, including selection of outcomes important to patients. We used a
random effects model to obtain pooled incidence rate ratios (IRR) and, when feasible, conducted subgroup
analyses (defined a priori) based on age, frequency of screening, family history, ethnicity, and
socioeconomic level, as well as a sensitivity analysis based on the risk of bias. The quality of the evidence
was assessed with the GRADE approach.
Results
Five randomised controlled trials, enrolling 721 718 men, were included. Studies varied with respect to
screening frequency and intervals, PSA thresholds for biopsy, and risk of bias. When considering the
whole body of evidence, screening probably has no effect on all-cause mortality (IRR 0.99, 95% CI 0.98 to
1.01; moderate certainty) and may have no effect on prostate-specific mortality (IRR 0.96, 0.85 to 1.08;
low certainty). Sensitivity analysis of studies at lower risk of bias (n=1) also demonstrates that screening
seems to have no effect on all-cause mortality (IRR 1.0, 0.98 to 1.02; moderate certainty) but may have a
small effect on prostate-specific mortality (IRR 0.79, 0.69 to 0.91; moderate certainty). This corresponds
to one less death from prostate cancer per 1000 men screened over 10 years. Direct comparative data on
biopsy and treatment related complications from the included trials were limited. Using modelling, we
estimated that for every 1000 men screened, approximately 1, 3, and 25 more men would be hospitalised
for sepsis, require pads for urinary incontinence, and report erectile dysfunction, respectively.
Conclusions
At best, screening for prostate cancer leads to a small reduction in disease-specific mortality over 10 years
but has does not affect overall mortality. Clinicians and patients considering PSA based screening need to
weigh these benefits against the potential short and long term harms of screening, including complications
from biopsies and subsequent treatment, as well as the risk of overdiagnosis and overtreatment.
Introduction
Prostate cancer is the second most common cancer and the fifth leading cause of cancer-associated
mortality among men worldwide.1 Screening for prostate cancer with serum prostate-specific antigen
(PSA) aims to detect prostate cancer at an early, intervenable stage amenable to curative treatment and
reduction in overall and disease-specific mortality.2 3 However, the evidence has so far not demonstrated
that screening for prostate cancer saves lives.4 5 Instead, screening may be associated with increased harms
such as overdiagnosis and complications of treatment for indolent disease.2 3 4 5 Nevertheless, screening
for prostate cancer remains highly controversial because of limitations in randomised trials including
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Our prior systematic review and meta-analysis that evaluated the effectiveness of prostate cancer screening
included five studies that enrolled 341 342 patients.5 In this 2013 Cochrane review we demonstrated that
PSA screening led to an increase in prostate cancer diagnoses but did not reduce overall nor disease-
specific mortality. Since the publication of this review, several of the included studies have reported
outcomes at extended follow-up. In addition, a new, large trial has been published.10 Therefore, the
effectiveness of prostate cancer screening based on the current best evidence is uncertain. In contrast to the
recently published USPSTF evidence report, we included and analysed all relevant screening trials using
data from the longest available follow-up.9
This systematic review is part of the BMJ Rapid Recommendations project, a collaborative effort from the
MAGIC research and innovation programme (www.magicproject.org) and The BMJ. The aim of the
project is to respond to new potentially practice changing evidence and provide a trustworthy practice
guideline in a timely manner.11 In our case, the stimulus was the recent Cluster Randomised Trial of PSA
Testing for Prostate Cancer (CAP),10 which randomised over 419 357 men at 573 primary care practices in
the United Kingdom to PSA screening versus usual management.10 In light of this new evidence, we
conducted an update of prior systematic reviews by our group to address the potential benefits and harms
of PSA based screening.4 5 This systematic review informed the parallel guideline published in a multi-
layered electronic format on bmj.com and MAGICapp (see box 1).
Box 1
Linked articles in this BMJ Rapid Recommendation cluster
Tikkinen KAO, Dahm P, Lytvyn L, et al. Prostate cancer screening with prostate-specific antigen
(PSA) test: a clinical practice guideline. BMJ 2018:362:k3581. doi:10.1136/bmj.k3581
Ilic D, Djulbegovic M, Jung JH, et al. Prostate cancer screening with prostate-specific antigen
(PSA) test: a systematic review and meta-analysis. BMJ 2018:362:k3519.
doi:10.1136/bmj.k3519
Systematic review and meta-analysis of all available randomised trials that assessed PSA
based screening for prostate cancer
Vernooij RWM, Lytvyn L, Pardo-Hernandez H, et al. Values and preferences of men for
undergoing prostate-specific antigen screening for prostate cancer: a systematic review. BMJ
Open 2018;0:e025470. doi:10.1136/bmjopen-2018-025470
Systematic review of the values and preference of men considering PSA screening
MAGICapp (https://app.magicapp.org/public/guideline/n32gkL)
Methods
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Protocol registration
The protocol for this systematic review was registered with PROSPERO (CRD42016042347).14
Search strategy
A trained medical librarian performed electronic searches of the Cochrane Central Register of Controlled
Trials (via Wiley), Web of Science, Embase, Scopus, OpenGrey, LILACS, Medline (via Ovid) and
PubMed (via National Library of Medicine) databases from their inception through to April 2018 (see
appendix 1). Additionally, we scanned the reference lists of published narrative and systematic reviews to
identify any potential studies not retrieved by our electronic search. In an effort to find unpublished
studies, we also hand searched abstracts from the annual meetings of American Urological Association,
American Society of Clinical Oncology, and European Urological Association from 2013 (the latest search
date of our most recent systematic review5) through to April 2018. To identify ongoing trials, we used the
International Clinical Trials Registry Platform (ICTRP) and ClinicalTrials.gov search portals.
The randomised controlled trials reported limited data on the harms of screening compared with no
screening. We therefore searched for follow-up evidence from the intervention arms of included trials and
follow-up publications. We used the Finnish arm of the European Randomised Study of Screening for
Prostate Cancer (ERSPC) for quality of life data15 and false positive rates.16 We estimated false negative
rates among men with a low PSA concentration from a follow-up cohort study of the Prostate Cancer
Prevention Trial.17 We extracted complication rates from prostate cancer treatment modalities from the
Prostate Testing for Cancer and Treatment (ProtecT) trial, which enrolled patients from the CAP trial who
were diagnosed with prostate cancer and randomised to active monitoring, radical prostatectomy, or radical
radiotherapy with hormones.18 Similarly, we obtained complication rates from biopsies from the Prostate
Biopsy Effects cohort study nested within the ProtecT trial.19 By modelling the likelihood of elevated PSA
levels, biopsies, cancer diagnoses, and treatment modalities (from the National Institute of Health
Surveillance, Epidemiology, and End Results Program),20 we estimated the absolute number of biopsy and
treatment related complications among men who underwent screening compared with those who did not.
Study selection
After removing duplicates, we imported all citations into Covidence (www.covidence.org). Two study
team members independently reviewed each citation and abstract according to a priori selection criteria.
For studies that advanced beyond this stage, two study team members then performed independent full-text
reviews. To determine eligibility, studies in non-English language were translated. References reporting on
the same trial were mapped to unique study identifiers.
We considered all randomised controlled trials in any language comparing PSA based screening with usual
care in men without a diagnosis of prostate cancer. Outcomes of interest were all-cause mortality, disease-
specific mortality, prostate cancer incidence and stage (categorised as localised and advanced cancer),
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quality of life, false positive and false negative results, and harms related to screening.
To assess the risk of bias of the selected studies, we used Cochrane’s risk of bias tool on an outcome-
specific basis.21 Two team members independently evaluated each randomised controlled trial across
several domains by relying on the information presented in the study report, available protocols, or
secondary publications. No assumptions were made. These domains included sequence generation and
concealment of allocation (selection bias), blinding of participants and personnel (performance bias),
blinding of outcome assessors (detection bias), and completeness of follow-up (attrition bias). In addition,
we assessed rates of adherence to the screening intervention and rates of contamination of the usual care
arm. For each domain, two individual team members judged whether the risk of bias was low, high, or
unclear. Any disagreements were reconciled by a third team member.
We rated the confidence in the estimates of effect for each outcome according to the GRADE (Grading of
Recommendations, Assessment, Development, and Evaluation) approach, taking into account study
limitations (risk of bias), inconsistency, imprecision, indirectness, and publication bias.22 For each
comparison, two team members independently rated the certainty of the effect estimates (that is, quality of
evidence) for each outcome as high, moderate, low, or very low. We resolved discrepancies by consensus
and, if needed, by arbitration by a third team member. The GRADE Summary of Findings table was
generated using the MAGICapp platform (www.magicapp.org). The trials varied in their duration of
follow-up from 10 to 20 years, and we pooled relative estimates of effect at the longest available follow-up
time. To generate absolute effect size estimates, we consistently used the baseline risk from the CAP trial
as it provided the most contemporary and therefore applicable control event rate in the absence of
screening.10 We calculated absolute effect size estimates at 10 years, which we determined to be an
appropriate time horizon to base individuals’ and panel members’ deliberations on. We also explored how
the absolute effect estimates varied according to baseline risks at different time horizons using data from
the ERSPC trial.
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We conducted meta-analyses and pooled the effect estimates using DerSimonian and Laird’s inverse of
variance random effects model and presented the results in forest plots.24 Following GRADE guidance,
statistical heterogeneity was determined using the Q statistic and I2 which was interpreted as follows; (a)
0-40% may not be important, (b) 30-60% may indicate moderate heterogeneity, (c) 50-90% indicates
substantial heterogeneity, and (d) 75-100% indicates considerable heterogeneity.25 Decisions about
downgrading for inconsistency were based on clinical relevance according to the clinical practice guideline
that this systematic review supports.26 27 Regardless of the observed statistical heterogeneity, and when
the evidence was available, we conducted the following pre-specified subgroup analyses: age (50-54, 55-
59, 60-64, 65-69, and ≥70 years), frequency of screening (periodic v one-time), family history (present v
absent), ethnicity (African descent v not), and socioeconomic level (higher v lower) using lower levels of
education (primary education only) as a proxy of lower socioeconomic status.28 For subgroup analyses, we
used the random effects meta-regression approach to test for interaction. We also conducted a sensitivity
analysis limited to studies assessed as having a lower risk of bias. We planned to use funnel plots to
examine the extent of publication bias for outcomes if there were 10 or more studies included, which was
not the case.14 Data were analysed using SAS (version 9.4, Cary, NC, US) and STATA software (Version
14.2, Texas, US). For all aspects of the review, we followed the guidelines of the Preferred Reporting
Items for Systematic reviews and Meta-Analyses (PRISMA) checklist.29
Results
These five randomised controlled trials enrolled a total of 721 718 participants. The CAP study was
conducted in the United Kingdom, recruiting 573 primary care practices.10 The remaining four trials
included the ERSPC study,31 the Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening trial
conducted in the US,32 and trials conducted in Canada (Quebec)33 and Sweden (Stockholm).23 The
ERSPC study was a multicentre study across eight European countries.31
Among the included studies, the age of men enrolled ranged from 40 to 80 years. Screening methods
included PSA alone and PSA combined with digital rectal examination. PSA thresholds to indicate further
investigation via biopsy differed across studies, as did the screening interval (varying from one-time, every
two years or more, to annual screening). Table 1 and appendix 2 provide additional details of the included
studies.
All included studies were assessed as being potentially susceptible to performance bias because of the
nature of the intervention. The CAP study had flawed allocation concealment (general practices consented
after randomisation and group assignment) and adherence (only 40% of those randomised to screening
adhered to it).10 The PLCO study had poor allocation concealment and major contamination.32 The two
other smaller studies had further issues across several domains.23 33 Figure 2 summarises the risk of bias
assessment for each study. The summary of findings for the entire body of evidence is detailed in table 2.
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Of the five included studies, only the ERSPC study was found to have a lower risk of bias than the rest of
the evidence from other trials.31 We therefore present a sensitivity analysis based on selected evidence
from ERSPC in table 3.
Effect of PSA screening on prostate-specific mortality PSA screening may have little or no effect on
prostate cancer-specific mortality based on five trials reporting this outcome (IRR 0.96 (0.85 to 1.08),
I2=58%, low quality evidence; fig 4, table 2).10 23 31 32 33 This corresponds to zero fewer deaths from
prostate cancer (95% CI 0 fewer to 0 more) per 1000 participants screened. We downgraded the quality of
evidence for risk of bias as well as clinically important inconsistency.
Effect of PSA screening on incidence of prostate cancer Based on data from four trials, screening may
increase the detection of prostate cancer of any stage (IRR 1.23 (1.03 to 1.48), I2=99%, low quality
evidence; fig 5, table 2).10 23 31 32 This corresponds to seven more diagnoses of prostate cancer (95% CI 1
more to 15 more) per 1000 men screened. We downgraded the quality of evidence for risk of bias as well
as clinically important inconsistency.
PSA screening may increase the detection of localised (stage I and II) prostate cancer based on evidence
from three randomised controlled trials (risk ratio (RR) 1.39 (1.09 to 1.79), I2=99%, low quality evidence;
fig 6, table 2).10 31 32 This corresponds to seven more localised prostate cancers diagnosed (95% CI 2
more to 15 more) per 1000 participants screened. We downgraded the quality of evidence due to risk of
bias and clinically important inconsistency. Based on the same body of evidence, PSA screening may
modestly decrease the incidence of advanced prostate cancer (stage III and IV) (RR 0.85 (0.72 to 0.99),
I2=87%, low quality evidence; fig 7, table 2). This corresponds to two fewer men diagnosed with advanced
prostate cancer (95% CI 4 fewer to 0 fewer) per 1000 men screened. We downgraded the quality of
evidence for risk of bias and clinically important inconsistency.
Subgroup analysis
We found no evidence of a subgroup effect according to age or screening frequency (appendix 3), but we
did find a subgroup effect according to risk of bias (lower versus higher risk of bias).
Other outcomes
Only a single trial, the Finnish arm of the ERSPC trial15 provided data on quality of life when comparing
PSA screening with no screening.15 31 This was based on a random sample of participants (n=1088) from
both trial arms excluding men with a subsequent diagnosis of prostate cancer. Using the SF-6D instrument
and a scale from 0 to 1.0 (with higher values indicating better quality of life), there was no difference
between the two arms (mean difference 0.01 (95% CI 0.01 lower to 0.02 higher), low quality evidence;
table 2). We downgraded the quality of evidence for risk of bias and indirectness.
Only the PLCO and CAP studies reported complications following biopsy.10 32 The PLCO study reported
75 complications from biopsies, including 29 infectious and 48 non-infectious adverse events. The CAP
study reported three biopsy related complications (1 attributed to biopsy, 2 post-biopsy). Based on the
Prostate Biopsy Effects cohort study, rates of biopsy-related complications ranged from 93%
19
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(haematospermia) to 1.4% (hospital readmissions, most commonly for sepsis)19: this corresponds to 94
men and one more man, respectively, per 1000 men screened. Graded by severity, 64.6% (95% CI 61.6%
to 67.8%) experienced minor complications, 31.8% (28.8% to 35.1%) had moderate complications, and
1.4% (0.8% to 2.4%) had major complications. There were no biopsy related deaths. At six years after
active monitoring, radical surgery, and local radiation, rates of urinary incontinence were 8%, 17%, and
4%, respectively, and rates of erectile dysfunction were 70%, 83%, and 73%, respectively (table 2).18
Comparing screened and unscreened men, we estimated there would be three more men per 1000 screened
presenting with urinary incontinence and 25 more men per 1000 screened with erectile dysfunction
(appendix 4).
Approximately two thirds of men with an elevated PSA level can expect a false positive test result,
meaning they will not be diagnosed with prostate cancer.16 Approximately 15% of men with a PSA level
<4 ng/mL will harbour prostate cancer of any grade consistent with a false negative result. Clinically
meaningful disease with a Gleason score ≥7 can be expected in 2.3% of men with a PSA level <4 ng/mL (
table 2).17
Effect of PSA screening on all-cause mortality Based on the ERSPC trial, PSA screening probably has
no effect on all-cause mortality (IRR 1.0 (0.98 to 1.02), moderate quality evidence; table 3). This
corresponds to zero fewer deaths of any cause (95% CI 3 fewer to 3 more) per 1000 participants screened.
We downgraded the quality of evidence for risk of bias.
Effect of PSA screening on prostate-specific mortality PSA screening probably has a small effect on
prostate cancer-specific mortality (IRR 0.79 (0.69 to 0.91), moderate quality evidence; table 3). This
corresponds to one fewer death from prostate cancer (95 CI 1 fewer to 0 fewer) per 1000 participants
screened. We downgraded the quality of evidence for risk of bias. When using the ERSPC control event
rate at 13 years31 or that of the Göteborg arm of the ERSPC trial34 at 18 years, this translates to one fewer
(95% CI 1 fewer to 2 fewer) or three fewer (95% CI 1 fewer to 4 fewer) deaths from prostate cancer,
respectively, per 1000 men screened.
Effect of PSA screening on incidence of prostate cancer Screening probably increases the detection of
prostate cancer of any stage (IRR 1.57 (1.51 to 1.62), moderate quality evidence; table 3). This
corresponds to 18 more diagnoses of prostate cancer (95% CI 16 more to 20 more) per 1000 men screened.
We downgraded the quality of evidence for risk of bias. Results of this analysis changed little when the
Stockholm trial was excluded in a sensitivity analysis.23 Screening probably also increases the incidence
of localised (stage I and II) prostate cancer (RR 1.75 (1.68 to 1.82)), corresponding to 14 more per 1000
(95% CI 13 more to 16 more), and probably decreases the incidence of advanced (stages III and IV)
prostate cancer (RR 0.75 (0.69 to 0.82)), corresponding to three fewer per 1000 men screened (95% CI 4
fewer to 2 fewer).
Other outcomes Since all available data for these outcomes were from the ERSPC trial the results of the
sensitivity analysis were the same as that of the main analysis.
Discussion
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Based on moderate and low quality evidence, PSA screening seems to increase the detection of prostate
cancer of any stage, increases the detection of stage I and II prostate cancer, and slightly decreases the
detection of stage III and IV prostate cancer. At the same time, it probably modestly reduces prostate
cancer specific mortality but has no effect on overall mortality. While findings from the ERSPC trial
reflects a 21% relative risk reduction of prostate cancer-specific mortality (95% CI 9.0% to 31.0%), this
corresponds to only one less death from prostate cancer (95% CI 1 fewer to 0 fewer) per 1000 men
screened. Meanwhile, PSA screening is associated with considerable biopsy-related and cancer treatment-
related complications. We estimated that, for every 1000 men screened, approximately one, three, and 25
more men will be hospitalised for sepsis, require pads for urinary incontinence, and report erectile
dysfunction, respectively.
The major limitation of this review stems from the included trials themselves. All trials had
methodological limitations that lowered the confidence of their effect size estimates, as summarised in
tables 2 and 3. Except for overall mortality, we found evidence of considerable inconsistency for each
pooled analysis, prompting us to further downgrade the quality of the evidence. The key issue was
considerable clinical and methodological heterogeneity across trials and within the arms of the ERSPC
trial itself. The limited number of available trials precluded many planned secondary analyses of observed
inconsistencies. Potential sources of heterogeneity include different screening intensity (one-time screen in
CAP versus multiple rounds in other trials), different screening intervals (annual in PLCO versus every 2-4
years in different arms of the ERSPC trial, for example), different PSA biopsy thresholds (ranging from
2.5 to 4 ng/mL), as well as various degrees of adherence and control group contamination. In contrast to
other systematic review authors,35 36 we chose not to adjust for these inconsistencies as this would
introduce additional uncertainty. Second, the reported harms evidence was not based on a comprehensive
evaluation of the published literature, but instead used any available follow-up evidence from the
intervention arms of the included trials and respective follow-up publications. This data was used to
estimate the absolute number of biopsy and treatment related complications among men who underwent
screening versus those who did not (see also infographic in linked Rapid Recommendation article12 and
appendix 4).
The most relevant study for comparison is the systematic review and evidence report referenced by the
recently published United States Public Services Task Force (USPSTF) recommendations on prostate
cancer screening, which differs from our review in several important ways.9 38 First, the CAP trial—the
largest and most recent trial of PSA screening to date—is missing from their evidence report. While the
USPSTF guideline developers gave it formal consideration, the CAP trial was published after the
USPSTF’s literature search cut-off date of 1 February 2018. Second, the authors excluded three older,
“poor quality” trials based on methodological grounds. Reasons included low adherence to the screening
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intervention, uncertain levels of contamination in the control arm, and lack of blinding of outcome
assessors. While we appreciate these methodological issues, their severity did not prompt us to disregard
two of the three studies entirely as the USPSTF Task Force did. We excluded the Norköpping trial on a
number of methodological limitations, including quasi-randomisation.30 Third, in contrast to the task
force’s evidence report, we meta-analysed the entire body of evidence. This approach allowed us to
evaluate for inconsistency between individual trials by conducting subgroup analyses (based on age and
screening intervals) and a sensitivity analysis for risk of bias. Fourth, another major distinction lies in how
we handled the control event rates. For the presentation of absolute effects in our summary of findings, we
chose to draw this event rate from the CAP trial because it provides the most contemporary and broadly
applicable estimation of what would occur in the absence of screening. These numbers were ascertained at
a 10-year time horizon (while the effect estimates were pooled at the longest follow-up available in the
included trials). This contributes to the lower absolute effect size estimates that we report compared with
those reported by the USPSTF, but were deemed the most appropriate to inform the accompanying Rapid
Recommendation panel deliberations.12
Prostate-specific antigen (PSA) screening leads to increased prostate cancer diagnoses. Although
it is recommended by some clinical practice guidelines, PSA screening remains controversial
It is unclear whether screening improves overall and disease-specific mortality, the most critical
outcomes for patients, or whether the overall benefits of screening outweigh the potential harms
and costs of overdetection and overtreatment
The recent publication of a large cluster-randomised controlled trial provides new evidence
At best, screening for prostate cancer may result in a small absolute benefit in disease-specific
mortality over 10 years but does not improve overall mortality
These benefits need to be weighed against the potential short and long term harms of PSA
screening, including complications from biopsies and subsequent treatment and the risk of
overdiagnosis and overtreatment
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Web Extra.
Extra material supplied by the author
Appendix 2: Characteristics of patients and trials included in the systematic review of the effects of
PSA screening
Appendix 3: Subgroup analyses based on age, screening frequency, and risk of bias
Appendix 4: Modelling approach used to estimate the absolute number of biopsy and treatment
related complications
Notes
Contributors: DI, MD, and PD were involved in the conception and design of the review. DI, MD, AC, and
PD developed the search strategy and performed study selection. DI, MD, JHJ, ECH, and PD extracted
data from included studies. QZ, TA, and PD were involved in the data analysis. DI, MD, JHJ, ECH, QZ,
TA, and PD were involved in the interpretation and discussion of results. All authors drafted the
manuscript, contributed to the drafting of the review, and revised it critically for important intellectual
content. All authors approved the final version of the article. All authors had access to all of the data in the
study and can take responsibility for the integrity of the data and the accuracy of the data analysis. PD is
guarantor.
Funding: This research received no specific grant from any funding agency in the public, commercial, or
not-for-profit sectors.
Competing interests All authors have completed the Unified Competing Interest form (available on request
from the corresponding author) and declare: no support from any organisation for the submitted work; no
financial relationships with any organisations that might have an interest in the submitted work in the
previous three years; no other relationships or activities that could appear to have influenced the submitted
work.
Transparency: DI and PD affirm that the manuscript is an honest, accurate, and transparent account of the
study being reported; no important aspects of the study have been omitted.
Patient involvement: As further described in the Methods section, three patient representatives helped
inform the questions, outcomes and thresholds of clinical importance for this systematic review.
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Fig 1
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Table 1
Characteristics of studies included in the systematic review
No of men
Setting, Enrolment Study randomised Screening Screening Prim
Study country criteria conducted (intervention/control) method frequency outc
10
CAP Cluster Men aged 2001- 195 912/219 445* PSA. If One-time Pro
RCT, 50-69 2009, 10 PSA ≥3 screening can
multicentre, years year ng/mL spe
UK follow-up standardised mor
prostate
biopsy
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Fig 2
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Table 2
Summary of findings from pooled analysis of all eligible trials (n=5)
Certainty in
Absolute effect estimates
effect
Study results Difference (95% estimates
No PSA
Outcome and and CI) (quality of Plain text
screening screening
timeframe measurements evidence) summary
Mortality and cancer incidence at 10 years*
All-cause IR 0.99 (95% 129/1000 128/1000 1 fewer (3 fewer to Moderate PSA
mortality CI 0.98 to men men 1 more) (serious risk of screening
1.01) based on bias†) probably has
data from 675 little or no
232 patients in effect on all-
4 studies. cause
Follow-up 10- mortality
20 years
Prostate IR 0.96 (0.85 3/1000 3/1000 0 (0) Low (serious PSA
cancer to 1.08) based men men risk of bias and screening may
mortality on data from inconsistency‡) have little or
721 718 no effect on
patients in 5 prostate
studies. cancer
Follow-up 10- mortality
20 years
Incidence of IR 1.23 (1.03 32/1000 39/1000 7 more (1 to 15 Low (serious PSA
prostate to 1.48) based men men more) risk of bias and screening may
cancer (any on data from serious increase
stage) 675 232 inconsistency detection of
patients in 4 (resulting in prostate
studies. large cancer (any
Follow-up 10- imprecision)§) stage)
20 years
Incidence of RR 1.39 (1.09 19/1000 26/1000 7 more (2 to 15 Low (serious PSA
localised to 1.79) based men men more) risk of bias and screening may
prostate on data from serious increase
cancer (stages 647 751 inconsistency detection of
I and II) patients in 3 (resulting in localised
Open in a separate window
†
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†
Risk of bias, serious: inadequate concealment of allocation during randomisation resulting in potential for selection
bias; inadequate or lack of blinding of participants and personnel, resulting in potential for performance bias; some
contamination. Imprecision, not serious: lower border of confidence interval compatible with a relevant yet small
clinical risk reduction.
‡
Risk of bias, serious: inadequate concealment of allocation during randomisation resulting in potential for selection
bias; inadequate or lack of blinding of participants and personnel, resulting in potential for performance bias; some
contamination. Inconsistency, serious: I2 53%; ERSPC trial shows significant reduction while all other trials show no
significant difference.
§
Risk of bias, serious: inadequate concealment of allocation during randomisation resulting in potential for selection
bias; inadequate or lack of blinding of participants and personnel, resulting in potential for performance bias; some
contamination. Inconsistency, serious: confidence intervals of some of the studies do not overlap with those of most
included studies or the point estimate of some of the included studies. Imprecision, not serious: we did not downgrade
for imprecision because it resulted from inconsistency.
¶
Risk of bias, serious: inadequate concealment of allocation during randomisation resulting in potential for selection
bias; inadequate or lack of blinding of participants and personnel, resulting in potential for performance bias;
inadequate or lack of blinding of outcome assessors, resulting in potential for detection bias; some contamination.
Inconsistency, serious: confidence intervals of some of the studies do not overlap with those of most included studies
or the point estimates of some of the included studies.
**
Risk of bias, serious: based on cross-sectional analysis of random sample, self-reported measure of health related
quality of life. Indirectness, serious: patients without prostate cancer were excluded from the screening arm (health
related quality of life of patients screened with no diagnosis of prostate cancer may differ).
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Table 3
Summary of findings from lower risk of bias data (sensitivity analysis based on ERSPC trial)
†
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†
Risk of bias, serious: inadequate concealment of allocation during randomisation resulting in potential for selection
bias; inadequate or lack of blinding of participants and personnel, resulting in potential for performance bias; some
contamination.
‡
Risk of bias, serious: inadequate concealment of allocation during randomisation resulting in potential for selection
bias; inadequate or lack of blinding of participants and personnel, resulting in potential for performance bias;
inadequate or lack of blinding of outcome assessors, resulting in potential for detection bias; some contamination.
§
Risk of bias, serious: based on cross-sectional analysis of random sample, self-reported measure of health related
quality of life. Indirectness, serious: patients without prostate cancer were excluded from the screening arm (health
related quality of life of patients screened with no diagnosis of prostate cancer may differ).
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Fig 3
Forest plot showing the incidence rate ratio (IRR) for all-cause mortality for PSA screening v control groups.
Horizontal bars denote 95% CIs. Studies are represented as squares centred on the point estimate of the result of
each study. The area of the square represents the weight given to the study in the meta-analysis. The pooled IRR
was calculated by DerSimonian–Laird random effects model. The diamond represents the overall estimated effect
and its 95% CI
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29/5/2021 Prostate cancer screening with prostate-specific antigen (PSA) test: a systematic review and meta-analysis
Fig 4
Forest plot showing the incidence rate ratio (IRR) for prostate-specific mortality for PSA screening v control
groups. Horizontal bars denote 95% CIs. Studies are represented as squares centred on the point estimate of the
result of each study. The area of the square represents the weight given to the study in the meta-analysis. The
pooled IRR was calculated by DerSimonian–Laird random effects model. The diamond represents the overall
estimated effect and its 95% CI
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29/5/2021 Prostate cancer screening with prostate-specific antigen (PSA) test: a systematic review and meta-analysis
Fig 5
Forest plot showing the incidence rate ratio (IRR) for the incidence of prostate cancer for PSA screening v control
groups. Horizontal bars denote 95% CIs. Studies are represented as squares centred on the point estimate of the
result of each study. The area of the square represents the weight given to the study in the meta-analysis. The
pooled IRR was calculated by DerSimonian–Laird random effects model. The diamond represents the overall
estimated effect and its 95% CI
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29/5/2021 Prostate cancer screening with prostate-specific antigen (PSA) test: a systematic review and meta-analysis
Fig 6
Forest plot showing relative risk (RR) for the incidence of localised (stages I and II) prostate cancer for PSA
screening v control groups. Horizontal bars denote 95% CIs. Studies are represented as squares centred on the
point estimate of the result of each study. The area of the square represents the weight given to the study in the
meta-analysis. The pooled RR was calculated by DerSimonian–Laird random effects model. The diamond
represents the overall estimated effect and its 95% CI
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Fig 7
Forest plot showing relative risk (RR) for the incidence of advanced (stages III and IV) prostate cancer for PSA
screening v control groups. Horizontal bars denote 95% CIs. Studies are represented as squares centred on the
point estimate of the result of each study. The area of the square represents the weight given to the study in the
meta-analysis. The pooled RR was calculated by DerSimonian–Laird random effects model. The diamond
represents the overall estimated effect and its 95% CI
Articles from The BMJ are provided here courtesy of BMJ Publishing Group
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