Operating Manual - Epmotion 5073
Operating Manual - Epmotion 5073
nual
eEN)
manual
epMotion® 5073
Hardware manual
Copyright© 2014 Eppendorf AG, Hamburg. All rights reserved, including graphics and images. No part of
this publication may be reproduced without the prior permission of the copyright owner.
Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.
Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.
The software of the device (firmware) contains open source software. License information is available on
request from Eppendorf AG.
Only for epMotion M5073, M5073c, 5075m and 5075mc: Limited Use Label License NOTICE TO
PURCHASER; LIMITED LICENSE FOR RESEARCH USE ONLY.
This product and its use may be covered by one or more patents owned by Gen-Probe Incorporated. The
purchase price for this product includes only limited, nontransferable rights under certain claims of certain
patents owned by Gen-Probe Incorporated to use this product for research purposes only. No other rights
are conveyed. Purchaser is not granted any rights under patents of Gen-Probe Incorporated to use this
product for any commercial use. Further information regarding purchasing a license under patents of
Gen-Probe Incorporated to use this product for any other purposes, including, without limitation, for
commercial use, may be obtained by contacting Gen-Probe Incorporated, Attn: Business Development
Department, 10210 Genetic Center Drive, San Diego, California 92121-4362, U.S.A.
5073 900.850-02/112014
Table of contents
epMotion® 5073 3
English (EN)
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.5 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.6 Change history . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.1.1 epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.1.2 EasyCon Tablet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.2.1 epMotion M5073. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.2.2 epMotion P5073 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.3.1 Mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.3.2 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.3.3 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.3.4 ThermoMixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.3.5 Magnetic separation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.3.6 CleanCap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.1.1 Intended use of epMotion P5073 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.1.2 Intended use of epMotion M5073. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.5 Danger symbols and safety equipment on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
4.2 Installing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5 Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1 Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1.1 Design of the epMotion M5073 worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1.2 Design of the epMotion P5073 worktable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.2 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.3 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.3.1 Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.3.2 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.3.3 Equipping the thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Table of contents
4 epMotion® 5073
English (EN)
5.4 ThermoMixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.4.1 Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.4.2 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.4.3 Equipping the ThermoMixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.5 Magnetic finger module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.5.1 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.5.2 Equipping the magnetic finger module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.6 CleanCap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.6.1 HEPA filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.6.2 UV lamp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.7 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.7.1 Dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.7.2 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
6 Dispensing liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.1 Basic procedure for dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.1.1 Operating principle of dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.1.2 Liquid aspiration up to the remaining volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
6.1.3 Bottom tolerance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
6.1.4 Transporting and dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.2 Dispensing modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.2.1 Dispensing mode: Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.2.2 Dispensing mode: Multidispense . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.2.3 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
6.3 Special features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
6.3.1 Liquid aspiration from vessel base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
6.3.2 Liquid aspiration from high vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
6.3.3 Liquid dispensing at the height of the vessel edge. . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
8 Labware accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
8.1 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
8.1.1 Rack for 24 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
8.1.2 Rack for 96 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
8.1.3 Rack LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Table of contents
epMotion® 5073 5
English (EN)
9 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
9.1 First steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
9.1.1 Switching on the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
9.1.2 Using the EasyCon Tablet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
9.1.3 Using the epMotion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
9.2 Equipping the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
9.2.1 Displaying the equipping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
9.2.2 Placing tools on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
9.2.3 Placing the epT.I.P.S. Motion on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
9.2.4 Placing the waste container on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
9.2.5 Placing the labware on the worktable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
9.3 Executing an application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
9.3.1 Executing a test run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
9.3.2 Starting the application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
9.3.3 Controlling the application run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
9.4 Completing your work. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
9.4.1 Cleaning up the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
9.4.2 Switching off the epMotion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
10.1 Decontamination and cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
10.1.1 Carrying out the wipe decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
10.1.2 Carrying out the spray decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
10.1.3 Carrying out the UV irradiation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
10.1.4 Autoclaving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
10.3 Servicing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
10.4 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
10.5 Carrying out firmware updates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
10.6 Checking the dispensing volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
10.7 Maintaining the dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
10.7.1 Exchanging the sealing rings on the eight-channel dispensing tool . . . . . . . . . . . . . . 92
10.8 Customer service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Table of contents
6 epMotion® 5073
English (EN)
11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
11.1 Error search. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
11.2 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
11.2.1 Optical sensor error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
11.2.2 Dispensing error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
11.2.3 Software error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Operating instructions
epMotion® 5073 7
English (EN)
1 Operating instructions
1.1 Using this manual
Your epMotion operating manual consists of hardware instructions and software instructions. Separate
instructions exist for optional software modules.
The operating manual is part of the product.
The current version of the operating manual can be found on our website: www.eppendorf.com.
Read the operating manual in full before using the device.
Store the operating manual at an easily accessible location.
The device may only be transferred with the operating manual.
If the operating manual is lost, replace it immediately. Please contact Eppendorf AG for further details.
The safety instructions of this operating manual indicate the following danger symbols and danger levels:
Depiction Meaning
1. Actions in the specified order
2.
Actions without a specified order
• List
Text Display text or software text
Additional information
CSV
Comma-separated value
DNA
Deoxyribonucleic acid
DWP
Deepwell plate
epT.I.P.S.
Eppendorf Totally Integrated Pipetting System
HEPA
High efficiency particulate air (filter)
LH
Liquid Handling
LIMS
Laboratory information management system
MTP
Micro test plate
PCR
Polymerase chain reaction
PDF
Portable document format
TMX
ThermoMixer
USB
Universal serial bus
UV
Ultraviolet radiation
Operating instructions
epMotion® 5073 9
English (EN)
1.5 Glossary
Application
Program for a specific application. An application includes the procedure and the equipping of the
worktable.
CleanCap
Optional epMotion equipment. The CleanCap includes a HEPA filter and at least one UV lamp.
Destination labware
Rack or plate with destination positions.
Destination position
Position in the labware that exclusively is destination of liquid transfers.
Dispensing tool
Tool that aspirates and dispenses liquid. Single-channel dispensing tools and eight-channel dispensing
tools are available for various volume ranges.
EasyCon Tablet
Eppendorf EasyCon™ Tablet. You can control your epMotion using the tablet and the epBlue software.
epBlue
Software for creating and administering applications and labware. The epMotion is controlled using the
epBlue software.
epT.I.P.S. Motion
Pipette tips of the epMotion. epT.I.P.S.® Motions are available with or without a filter.
Filling volume
Maximum volume of a labware. The filling volume is higher than the working volume. The epMotion
removes liquid from the labware up to the filling volume.
Gripper
Tool that transports labware.
Operating instructions
10 epMotion® 5073
English (EN)
Height adapter
Adapter for low labware. The height differences between the labware will be offset to decrease the tool
holder paths, and therefore decrease the cycle time of the application.
HEPA filter
Air filter. The filter prevents particles such as dust and germs from the environment entering the epMotion
workspace.
Labware
Racks, plates, tips, etc. that are placed on the worktable.
Location
Area where labware is placed on the worktable.
MagSep
System for nucleic acid preparation with the epMotion. The MagSep consists of a magnetic finger module,
a ThermoMixer, a PrepRack and reagients.
Procedure
Sequence of commands that are executed consecutively. Part of an application.
Rack
Mount for tubes or pipette tips.
Random error
Precision. Standard deviation of the average value of the dispensed volumes.
Remaining volume
Volume that cannot be removed from a vessel. Caused by the distance from the pipette tip to the vessel
base, defined in the software, and the defined immersion depth of the pipette tip in the liquid. The pipette
tip cannot aspirate the volume. The remaining volume depends on the vessel geometry.
Reservoir
Reservoirs are used to hold reagents. Reservoirs are hung in a ReservoirRack or placed directly on the
worktable.
SafeRack
Rack with ep.T.I.P.S. Motion pipette tips. The SafeRack features a partition that prevents the contamination
of adjacent tips. If you would like to use tips several times, use the SafeRacks.
Operating instructions
epMotion® 5073 11
English (EN)
Source labware
Rack or plate with source positions.
Source position
Position in the labware that exclusively is a source of liquid transfers.
Systematic error
Accuracy. Deviation of the average value of the dispensed volumes from the selected volume.
Thermal module
Heat plate for labware. The heat plate is integrated in the worktable.
Thermoadapter
Heat conducting adapter for holding plates.
Thermoblock
Thermoadapter that is permanently connected to a PCR plate or PCR vessel.
Thermorack
Temperable rack for smaller vessels, e.g., Safe-Lock tubes for 0.5 mL, 1.5 mL or 2 mL.
Tube
Tube or single well of a plate.
Working volume
Filling volume minus displacement by the immerging pipette tip. The epMotion fills the vessels up to the
working volume.
Worktable
epMotion work surface that labware and tools are placed on. The epMotion worktable will be shown as the
epBlue worktable in the software.
Change history of the epMotion 5073 hardware manual, order no. 5073 900.850.
2 Product description
2.1 Main illustration
2.1.1 epMotion
Abb. 2-1: epMotion main illustration
10 9 8 6 5 10 9 8 5
4 5
Ethernet USB 1
1 Interfaces 4 Ethernet
Port for the cable towards the EasyCon Tablet.
2 Mains/power connection
5 USB
3 Mains/power switch
Port for a USB storage medium for firmware
updates.
Product description
epMotion® 5073 15
English (EN)
The EasyCon Tablet is used for controlling the epMotion. The EasyCon Tablet has a touch-sensitive color
display (touchscreen). A key pad will be shown on the display for data entry.
1 USB 2 Ethernet
Ports for a mouse with USB connection cable or a Port for the epMotion cable
USB storage medium
Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected
to the interfaces.
Product description
16 epMotion® 5073
English (EN)
2.3 Features
The epMotion 5073 is available with differing kinds of equipment. This manual describes all of the available
equipment.
2.3.2 Gripper
A gripper is available for the epMotion. The gripper stacks and transports labware.
2.3.4 ThermoMixer
The epMotion M5073 and epMotion M5073c (with CleanCap) are equipped with one thermal module.
2.3.6 CleanCap
Optionally, the epMotion is equipped with a CleanCap.
The CleanCap contains a UV lamp and HEPA filter.
3 Safety
3.1 Intended use
3.1.1 Intended use of epMotion P5073
The device is intended for use in laboratories for research, development, and industrial and routine work,
as well as for training purposes. The areas of applications include – but are not limited to – the fields of life
sciences, biotechnology, chemistry and clinical research.
The epMotion P5073 automated pipetting system is designed for contamination-free, precise and accurate
measuring and transferring of liquids. The autoclavable dispensing tools work in a volume range of 1 μL to
1000 μL.
Although the product is not registered with the FDA, the device meets the basic, essential requirements of
the EC Directives and Standards that are listed in the declaration of conformity.
These automated pipetting systems are exclusively intended for use indoors and may only be used by
skilled specialists who have received adequate training.
The device is intended for use in laboratories for research, development, and industrial and routine work,
as well as for training purposes. The areas of applications include – but are not limited to – the fields of life
sciences, biotechnology, chemistry and clinical research.
The epMotion M5073 automated pipetting system is designed for contamination-free, precise and accurate
measuring and transferring of liquids. The autoclavable dispensing tools work in a volume range of 1 μL to
1000 μL.
With integrated ThermoMixer, this device will be used for applications that allow for mixing and incubation
with open vessels and which conduct a fully automatic nucleic acid purification with magnetic particles.
Although the product is not registered with the FDA, the device meets the basic, essential requirements of
the EC Directives and Standards that are listed in the declaration of conformity.
These automated pipetting systems are exclusively intended for use indoors and may only be used by
skilled specialists who have received adequate training.
Safety
20 epMotion® 5073
English (EN)
The specialists must have received training on this device. The training must have been conducted by
Eppendorf AG or an authorized partner of Eppendorf AG.
The specialists must have read thoroughly the operating manual of the device. The specialists must have
read thoroughly the operating manuals of all software components.
In the following cases, the designated protection of the device may be compromised. Liability for any
resulting property damage or personal injury is then transferred to the operator:
WARNING! Health risk in the event of skin contact with infectious substances.
Wear protective gloves.
WARNING! Danger due to flammable and infectious liquids in the waste container.
Pipette tips in the waste container may contain flammable or infectious liquids.
Wear personal protective equipment.
Handle the pipette tips and sample materials from the waste container in accordance with
the material safety data sheets, safety regulations and laboratory guidelines.
This section explains the warning symbols on the epMotion and the location of the safety equipment.
Abb. 3-1: Danger symbols and safety equipment on the epMotion 5073
1 1
2 2
3
4 4
Fig. 3-1: Danger symbols and safety equipment on the epMotion 5073
Abb. 3-2: Danger symbols on the epMotion P5073c and epMotion M5073c
UV
CAU TI ON
R I S K G RO U P 2
UV emitted from the device
Eye or skin irritation may
result from exposure
Use appropriate shielding
5
Ethernet USB
Fig. 3-2: Danger symbols on the epMotion P5073c and epMotion M5073c
Safety
24 epMotion® 5073
English (EN)
2 WARNING
The carrier may continue moving after the front panel has
been opened.
Wait until the carrier stops moving before reaching into
the device.
3 DANGER
Risk of burns from the hot surface.
Wait until the surface has cooled before touching it.
5 NOTICE
Danger due to UV radiation.
Wear personal protective equipment (PPE).
6 The label is located on the magnetic finger module and is
only visible to the authorized service.
WARNING
Danger due to powerful magnetic field.
Do not work with the device if you have a cardiac
pacemaker.
4 Installation
4.1 Selecting the location
Information on ambient conditions, dimensions and weights can be found in the technical data (see Weight/
dimensions on p. 101).
Select the location for the device according to the following criteria:
Electrical connections
• Mains connection in accordance with the name plate.
• The device mains/power switch and cutting unit of the power system circuit (e.g., FI protective switch)
can be accessed during operation.
Location
• The ambient conditions match the specifications in the technical data.
• The location is well ventilated.
• The location is protected from direct sunlight.
• The location is not next to heat sources, such as heaters or drier compartments.
• There is an adequate amount of space available for the device. The minimum distances to other devices
and walls is 6 cm.
• The device can be safely and easily operated at this location.
Workplace
• The lab bench is permanently mounted.
• The lab bench is designed for the weight of the device.
• The lab bench has a horizontal, plane work surface.
• The lab bench has a non-slip surface.
• The lab bench is vibration-free.
• There are no vibrating devices on the lab bench.
The epMotion may only be installed and commissioned by service technicians authorized by
Eppendorf.
5 Hardware
5.1 Worktable
5.1.1 Design of the epMotion M5073 worktable
Abb. 5-1: epMotion M5073 worktable
Detector
Diaphragm
Diaphragm Light source
Semi-transparent
mirror
Z Optics
Z0
On the carrier, the optical sensor is located to the right of the tool holder.
The optical sensor records the intensity of the reflected light using a lateral infrared light source,
semi-transparent mirror, fixed lens and the procedure in z-direction. Height z0 is defined as the point with
the highest intensity which enables the detection of the level in a vessel or the presence of labware.
The optical sensor can detect horizontal, plane surfaces. The surface of the liquid must be at an angle of
90° ± 3° to the optical axis.
The surfaces of liquids can be strongly bent by the vessel geometry or the physical properties of the liquid
or vessel. The optical sensor may not detect the level on strongly bent surfaces. If this is the case, the user
must enter the liquid volume.
The optical sensor has a detection limit for vessel levels. The detection limit depends on the vessel
geometry. Generally, levels of 3 mm or higher can be detected.
Hardware
epMotion® 5073 29
English (EN)
Information on setting the optical sensor, and a detailed description of all functions, can be
found in the software operating manual.
Based on the individual model, your epMotion can be equipped with one thermal module.
5.3.2 Configuration
Abb. 5-4: Thermal module
Tab. 5-1: Temperature control duration for labware with thermal module
Labware Water filling Cooling Heating Heating
on the thermal module volume per from 25°C to 4°C from 25°C to 37°C from 25°C to 95°C
well in μL
Temperature min Temperature min Temperature min
setting in °C setting in °C setting in °C
Thermal module 5 2 8
without labware
Thermoadapter with 96-well 50 3 7 38 5 104 11
twin.tec PCR plate skirted
Thermoblock with 96-well 50 3 6 37 4 101 10
twin.tec PCR plate skirted
Thermoadapter with 384-well 10 3 5 37 5 103 8
twin.tec PCR plate skirted
Thermoblock with 384-well 10 3 5 37 5 98 7
twin.tec PCR plate skirted
24-unit thermorack 350 3 24 37 16 101 35
with 0.5 mL Safe-Lock tubes
24-unit thermorack 1200 3 30 38 18 104 44
with 1.5 mL Safe-Lock tubes
24-unit thermorack 1700 3 25 37 17 100 40
with 2.0 mL Safe-Lock tubes
This information applies at an ambient temperature of 25 °C for the border positions in the labware. The
thermal modules were tempered at the target temperature before the labware was positioned.
Information on setting the thermal module and a detailed description of all functions can be
found in the "Operation" chapter of the software instructions.
Hardware
epMotion® 5073 31
English (EN)
When you use a thermoblock or thermoadapter, plates with uneven bases will be optimally tempered, e.g.
PCR plates.
5.4 ThermoMixer
Based on the individual model, your epMotion can be equipped with a ThermoMixer.
NOTICE! Contamination of samples and the device due to excessive mixing speed.
If excessive speeds are set, liquid splashes out of the vessels. Labware can free itself from the
holder and fly around.
Observe the maximum speeds for labware in the operating manual.
Hardware
32 epMotion® 5073
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5.4.2 Configuration
Abb. 5-5: ThermoMixer
Information on setting the ThermoMixer, and a detailed description of all functions, can be
found in the software instructions.
Based on the individual model, your epMotion can be equipped with a magnetic finger module.
5.5.1 Configuration
Abb. 5-6: Magnetic finger module
The magnetic finger module is used for isolating suspended magnetic particles.
The magnetic finger module has 3 fingers that can be moved horizontally. Strong permanent magnets are
located on each finger. The 3 magnetic fingers move toward the vessels via the lateral openings in the
PrepRack. The magnetic field separates the magnetic particles on the tube inner wall. The dispensing tool
can remove liquid from the vessels, or place liquid in the vessels, while the magnetic particles are held in
place.
For a complete nucleic acid preparation, you can use the MagSep kits by Eppendorf AG.
The geometry of the used vessels is important for the quality of the separation. Use 2.0 mL
Safe-Lock tubes.
5.6 CleanCap
The CleanCap is an optional equipment of the epMotion and can only be ordered in combination with a new
device. The CleanCap includes a HEPA filter and a UV lamp.
The HEPA filter can become dirty over time. Have the HEPA filter replaced by the authorized
service partner once per year.
The HEPA filter cleans the air in the epMotion workspace. The HEPA filter reduces the amount of particles
such as dust or germs.
The fan continuously draws air out of the ambience and channels it to the HEPA filter. The filtered air flow
will be routed to the epMotion workspace.
Prerequisites
• The epMotion is ready for operation.
1. Decontaminate the worktable and the lateral surfaces in the workspace of the epMotion.
2. Decontaminate the labware and dispensing tools.
3. Close the front hood.
4. Switch on the HEPA filter using the epBlue software.
5. Let the HEPA filter run for 5 min.
All of the air in the epMotion workspace has been filtered.
6. Equip the epMotion.
7. Close the front hood.
8. Start the application.
The HEPA filter remains switched on after the end of the application.
9. Start another application, or switch off the HEPA filter using the operating software.
Hardware
epMotion® 5073 35
English (EN)
5.6.2 UV lamp
The radiant flux of the UV lamp drops due to aging. Have the UV lamp replaced by an
authorized service partner once every 2 years.
The UV lamp decontaminates the worktable. The UV irradiation lasts 15 min. The UV lamp is controlled by
the software.
Prerequisites
• The epMotion is ready for operation.
1. Remove the labware, waste container and dispensing tools from the worktable.
2. Close the front hood.
3. Switch on the UV lamp using the software (refer to the epMotion software manual).
The UV lamp is switched off when the front hood is opened.
5.7 Tools
The tool holder holds a tool and releases it. The tool holder recognizes the tool by its gold contacts.
50
The dispensing tools are piston-stroke pipettes. The function of the dispensing tools is described in the
corresponding chapter (see Dispensing liquids on p. 39).
For the epMotion, single-channel dispensing tools and eight-channel dispensing tools, with 3 volume
ranges each, are available.
The dispensing tool errors can be found in the technical data (see p. 104).
Help on dispensing errors can be found in the "Maintenance" chapter (see Dispensing error on p. 96)
Hardware
epMotion® 5073 37
English (EN)
5.7.2 Gripper
The gripper transports labware. The gripper uses the mandrels on the gripper arms to hold the labware.
The gripper is put down on the gripper holder.
The gripper transports the following labware: The gripper does not transport the following
labware:
• Plate • Individual vessels
All plates are transported. Plates will be placed • Plate stack
on a worktable, stack, height adapter or The top plate will be transported from a plate
thermoadapter. stack.
• Thermoblock • epT.I.P.S. Motion Racks
Will be transported with the positioned plate. • Racks
The positioned plate alone will not be picked up • ReservoirRacks
by the gripper without the thermoblock. • Reservoirs and ReservoirRack modules
• Thermorack • Height adapter
Will be transported with the inserted reaction • Thermoadapter
vessels. The inserted reaction vessels will not be A plate will be transported if it is inserted in a
picked up by the gripper without the thermoadapter.
thermorack. • ReagentRack
• PrepRack
Will be transported with the inserted reaction
vessels. The inserted reaction vessels will not be
picked up by the gripper without the
thermorack.
• 300 mL Reservoir
• 400 mL Reservoir
Hardware
38 epMotion® 5073
English (EN)
Dispensing liquids
epMotion® 5073 39
English (EN)
6 Dispensing liquids
6.1 Basic procedure for dispensing
6.1.1 Operating principle of dispensing tools
Abb. 6-1: Piston-stroke pipette principle
basic position
end of blow-out
A B C
A Aspiration C Blow-out
The piston moves upward from the home position Dispenses the residual liquid
and aspirates liquid. Blow-out in the Pipette dispensing mode
Remaining stroke in the Multidispense dispensing
B Dispense
mode
Liquid dispensing.
Multidispense: Piston travels to the home position
in separate steps.
Pipette: Piston travels to the home position in one
step.
The dispensing tools are piston-stroke pipettes that function according to the air cushion principle.
If the piston moves upward in the dispensing tool, liquid will be aspirated into the pipette tip. If the piston
moves downward in the dispensing tool, liquid will be dispensed from the pipette tip. The pistons will be
moved by a motor in the tool holder.
0,7 mm 1 mm
Fig. 6-2: Liquid aspiration up to the remaining volume with a constant immersion depth
The pipette tip is immersed 3 mm into the liquid before liquid aspiration. The pipette tip moves downward
during liquid aspiration. The immersion depth of 3 mm remains constant while the liquid level decreases.
If the pipette tip reaches a distance of 1 mm to the vessel base, the pipette tip reduces its immersion depth
to 0.7 mm. If the liquid level continues to sink, the pipette tip will not aspirate any additional liquid. The
remaining volume remains in the vessel.
A remaining volume is present because the pipette tip has a minimum immersion depth in the liquid and a
minimum distance to the vessel base. Under normal conditions, the remaining volume is calculated using a
filling level of 1.7 mm.
The distance of the pipette tip to the vessel base can be changed in the software (refer to the epMotion
software manual).
If the liquid is aspirated up to the remaining volume, the bend of the liquid level can lead to
dispensing errors.
The bottom tolerance is the distance between the vessel base and the lower end of the pipette tip. The
default setting for the bottom tolerance is 1 mm. For 30 mL and 100 mL reservoirs, the default setting for
the bottom tolerance is 2.5 mm.
1. If you remove liquids over a pellet, you increase the bottom tolerance.
2. Do not decrease the bottom tolerance on uneven plates.
3. Check the set bottom tolerance when exchanging pipette tips, plates or vessels.
Dispensing liquids
epMotion® 5073 41
English (EN)
~ 3 - 4 mm ~ 6 - 8 mm
A B
Fig. 6-3: Transporting and dispensing liquid
The liquid from the pipette tip will be dispensed in the free jet. The liquid will be dispensed into the vessel
3 mm – 4 mm above the surface of the liquid. The pipette tip moves upward during dispensing. The
distance from the pipette tip to the surface of the liquid remains constant.
For liquid type Protein C, a distance of 5 mm from the pipette tip to the surface of the liquid is set in the
software. This setting takes into account the tendency of concentrated protein solutions to form foam.
Before the transport, liquid is retracted into the pipette tip. An area filled with air is created in the lower
part of the pipette tip. The liquid does not drip during transport.
Dispensing liquids
42 epMotion® 5073
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The epMotion can be used to dispense liquids in the Pipette and Multidispense modes.
In the Pipette mode, a defined volume of liquid is aspirated and fully dispensed.
Dispensing errors are smaller than in the mode Multidispense (see Dispensing tool errors on p. 104).
The epMotion executes a blow out in the Pipette mode. Residual liquid from the pipette tip will be
dispensed in the destination position. The blow out is part of the dispensing volume. The blow out can be
set in the software (refer to the epMotion software manual).
In the Pipette mode, the smallest dispensing volume for water is 1 μL.
In the Multidispense mode, liquid is aspirated and dispensed in the destination positions in defined partial
quantities.
Dispensing errors are larger than in the mode Pipette (see Dispensing tool errors on p. 104).
The epMotion carries out a remaining stroke in the Multidispense mode. Residual liquid from the pipette tip
is dispensed into the waste container or the source position. The residual liquid is not part of the
dispensing volume.
In the Multidispense mode, the smallest dispensing volume for water is 3 μL.
To create the same physical conditions for all dispensing steps, more liquid must be aspirated in the
Multidispense mode than the sum of the dispensing volumes. This is the result of the reverse stroke and the
remaining stroke.
Reverse stroke
• The reverse stroke occurs after liquid aspiration.
Part of the aspirated volume is immediately returned to the source position.
The reverse stroke is the same size for all liquids.
Remaining stroke
• After the reverse stroke, there is more liquid in the pipette tip than the amount required for the
dispensing steps. This additional aspiration volume will be dispensed as a remaining stroke after
dispensing.
An additional aspiration volume will be dispensed in the source position if the tips are not replaced
before each liquid aspiration.
The additional aspiration is dispensed in the waste container if the tips are replaced before each liquid
aspiration.
Dispensing liquids
epMotion® 5073 43
English (EN)
The volume for the additional aspiration depends on the dispensing tool; the software will take it into
account when calculating the required aspiration volume.
A 96-well plate should be filled with 10 μL water in each well via multidispense. The eight-channel
dispensing tool TM 50 will be used. Aspiration will be completed from one reservoir. The tips are not
changed before a new liquid aspiration.
6.2.3 Mixing
A mixing cycle consists of one upward movement and one downward movement of the piston in the
dispensing tool.
The aspiration level and dispensing level of the liquid can be defined during mixing. A defined height
should only be used for levels below the working volume as the vessel may overflow with larger levels.
During the mixing process, the pipette tip is in the liquid. The blow out will be executed above the surface
of the liquid after the mixing procedure.
Defined mixing speeds are defined in the liquid types. When using your own settings, determine the
optimal mixing speed via experiments.
Dispensing tool Mixing speed of lower Mixing speed of medium Mixing speed of upper
volume range in mm/s volume range in mm/s volume range in mm/s
TS 50 15 – 88 15 – 44 10 – 40
TM 50 15 – 88 15 – 44 10 – 40
TS 300 5 – 15 6 – 16 6 – 16
TM 300 2 – 11 2 – 11 2 – 11
TS 1000 4 – 15 4 – 15 4 – 15
TM 1000 4 – 15 4 – 15 4 – 15
The pipette tip can also be positioned above the vessel base at the height of the bottom tolerance. This
prevents the pipette tip from changing its position during liquid aspiration.
The filling level in the vessels may not exceed the working volume. If the filling level exceeds the working
volume, liquid may overflow.
Liquid aspiration from the vessel base is recommended in the following cases:
• For vessels ≤0.5 mL, 96-well plates and 384-well plates
• For plates with various filling volumes in the wells
• For vessels with low filling volumes
Liquid aspiration from the vessel base can be activated in the software using the Aspirate from bottom
option (refer to the epMotion software manual). The bottom tolerance depends on the labware. You can set
the bottom tolerance in the software.
A B
A Vessel filled up to the working volume B Vessel with maximum immersed tip before
liquid aspiration
Dispensing liquids
epMotion® 5073 45
English (EN)
Observe the following special features when using high vessels, e.g. 15 mL centrifuge tubes.
If you use vessels > 3 mL with high filling levels, do not aspirate liquids from the vessel base. This will result
in the outside of the pipette tip being wetted, which increases the risk of contamination and distorts the
dispensing result. For very large vessels with high filling levels, the entire pipette tip and the tip cone of the
dispensing tool will be wetted.
Fig. 6-6: Liquid aspiration from high vessels with 1000 μL, 50 μL and 300 μL pipette tips
50 μL tips and 300 μL tips do not reach as far into the vessels as 1000 μL tips. In high vessels, 50 μL and
300 μL tips do not reach the vessel base. If you use 50 μL and 300 μL tips, the remaining volume will be
larger than with 1000 μL tips.
Work with the 1000 μL dispensing tool when you use high vessels.
If you use the 50 μL dispensing tool or the 300 μL dispensing tool, and would like to reduce
the remaining volume, select the lower vessels.
Dispensing liquids
46 epMotion® 5073
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In the software, the pipette tip can be positioned at a height of 3 mm – 4 mm below the edge of the vessel
using the Dispense from top option. The height of the pipette tip does not change.
The term labware refers to epMotion consumables and accessories. Consumables are vessels, plates and
pipette tips. Accessories are adapters, blocks and racks. epBlue includes a definition for labware that is
placed on the epMotion worktable. The definition includes data on the geometry, tempering ability, volume
and bottom tolerance of the labware.
The epMotion can be used with a variety of labware from various manufacturers. Information
on how to expand the labware library can be found in the software operating manual.
Labware may not exceed an overall height of 126 mm. The software generates an error
message if this overall height is exceeded.
Abb. 7-1: Reaction vessel with lid
1.5 1
1.0
2
0.5
0.1
3
4
7.1 Tubes
Tubes are inserted into Racks. All tubes which fit in an epMotion Rack can be used.
The level measurement with the optical sensor is more difficult with 0.2 mL and 0.5 mL tubes.
7.2 Plates
Abb. 7-2: Plates: 96-well MTP on the left, 24-well MTP in the middle, 96-well DWP on the right
Fig. 7-2: Plates: 96-well MTP on the left, 24-well MTP in the middle, 96-well DWP on the right
Skirted plates can be placed directly at a worktable location. Plates can be placed on adapters.
Plates are transported with the gripper. Stacked plates will not be transported together.
Stacking plates
7.2.2 Microplates
Abb. 7-4: Microplate with 96 wells
A level measurement with optical sensor cannot be completed for an MTP with 384 wells. A level
measurement with optical sensor is not recommended for an MTP with 96 wells.
96-well PCR plates are available with a border (skirted), a semi-border (semi-skirted) or without a border
(unskirted).
96-well PCR plates with a semi-border or without a border can only be positioned and transported with the
Thermoblock PCR 96.
Tube plates are plates with individual vessels. Tube plates are available in various vessel layouts. Tube
plates are treated as normal plates.
Labware – vessels, plates and pipette tips
50 epMotion® 5073
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7.3 Reservoirs
Abb. 7-6: 100 mL and 30 mL Reservoirs Abb. 7-7: 400 mL Reservoir
Reservoirs make liquid available. Reservoirs are available in 30 mL, 100 mL and 400 mL sizes. Reservoirs
for 30 mL and 100 mL are placed in a ReservoirRack.
Observe the instructions for use for the epT.I.P.S. Motion Racks and epT.I.P.S. Motion Reloads.
Abb. 7-8: epT.I.P.S. Motion 1 000 μL, 50 μL, 300 μL
epT.I.P.S. Motion pipette tips, racks and trays are made of polypropylene (PP). The filter is made of
polyethylene (PE).
The optical sensor detects the size of the pipette tips via the Rack coding. The optical sensor also detects if
the pipette tips have a filter. However, the optical sensor does not use the coding to detect if the pipette tips
are in Racks, SafeRacks or in the TipHolder.
Labware – vessels, plates and pipette tips
52 epMotion® 5073
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epT.I.P.S. Motion Racks are specially designed for applications that require sterile work.
Tab. 7-2: epT.I.P.S. Motion Racks are available in the following sizes:
Size of the pipette tips Dispensing tool Color of the Rack
50 μL TS 50, TM 50 Light gray
300 μL TS 300, TM 300 Yellow
1000 μL TS 1000, TM 1000 Dark blue
Tab. 7-3: epT.I.P.S. Motion Racks are available in the following purity grades:
epT.I.P.S. Motion Racks Purity grade
Pipette tips with filter PCR clean
PCR and Sterile
Pipette tips without filter Eppendorf Quality
Sterile
Labware – vessels, plates and pipette tips
epMotion® 5073 53
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epT.I.P.S. Motion in SafeRacks are intended for multiple uses. If you use the pipette tips in an application,
use epT.I.P.S. Motion SafeRacks, for example, to remove buffers in several washing steps.
epT.I.P.S. Motion SafeRacks have a partition to separate pipette tips. During an application, used pipette tip
are returned to the SafeRack. A partition prevents residual liquid from contaminating adjacent pipette tips.
Contamination of samples due to repeated use is prevented because the software permanently allocates the
pipette tips within a specific application to a source position.
In the software, you can set whether pipette tips should be used multiple times (refer to the epMotion
software manual).
Use epT.I.P.S. Motion SafeRacks for a maximum of 6 usage cycles. One usage cycle consists of
aspiration, dispensing and ejecting the tip.
Tab. 7-4: epT.I.P.S. Motion SafeRacks are available in the following sizes:
Size of the pipette tips Dispensing tool Color of the Rack
50 μL TS 50, TM 50 Light gray
300 μL TS 300, TM 300 Yellow
1000 μL TS 1000, TM 1000 Dark blue
Tab. 7-5: epT.I.P.S. Motion SafeRacks are available in the following purity grades:
epT.I.P.S. Motion SafeRacks Purity grade
Pipette tips with filter PCR clean
Pipette tips without filter Eppendorf Quality
Labware – vessels, plates and pipette tips
54 epMotion® 5073
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Abb. 7-11: epT.I.P.S. Motion Reloads in individual packaging Abb. 7-12: epT.I.P.S. Motion Reloads and TipHolder
Fig. 7-11: epT.I.P.S. Motion Reloads in individual Fig. 7-12: epT.I.P.S. Motion Reloads and TipHolder
packaging
The pipette tips are in trays. The trays will be placed in reusable tip holders, which reduces waste because
no Racks are required.
Tab. 7-6: epT.I.P.S. Motion Reloads are available in the following sizes:
Size of the pipette tips Dispensing tool Tray color
50 μL TS 50, TM 50 Light gray
300 μL TS 300, TM 300 Yellow
1000 μL TS 1000, TM 1000 Dark blue
Tab. 7-7: epT.I.P.S. Motion Reloads are available in the following purity grades:
epT.I.P.S. Motion Reloads Purity grade
Pipette tips with filter PCR clean
PCR and Sterile
Pipette tips without filter Eppendorf Quality
Labware – vessels, plates and pipette tips
epMotion® 5073 55
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1
2
1 Tips 3 TipHolder
2 Tray
8 Labware accessories
Vessels and accessories may not exceed an overall height of 126 mm. If this overall height is
exceeded, the software generates an error message.
8.1 Racks
Racks are holders for mounting the same type of individual vessels.
Various Racks are available for vessels 12 mm – 17 mm in diameter. Racks are available in 2 heights. The
bores for the vessels are numbered.
The Racks cover the same area as plates. Racks can be positioned at any location.
Racks are coded. The optical sensor uses the code to detect if the Rack is correctly positioned.
The Rack for 96 vessels takes up 2 locations on the worktable. The Rack holds 96 vessels without lid with a
content of 1.5 mL or 2 mL.
Labware accessories
58 epMotion® 5073
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The Rack can be equipped with vessels with fastened lids, e.g. Safe-Lock tubes.
If you equip the Rack with vessels with fastened lids, leave each second row of the Rack empty.
Ensure that the lids do not cover the openings of adjacent vessels.
1. Place the Rack on the epMotion worktable so that the opening in the lower panel points toward the user.
2. Place the Rack on the positining pins of the two locations.
8.1.3 Rack LC
Abb. 8-3: Rack LC 100 μL
The Rack LC can hold 96 LightCycler capillaries with a 20 μL filling volume or 96 capillaries with a 100 μL
filling volume. The bores for both capillary sizes are arranged in an alternating pattern.
The Rack LC is labeled on both sides. The label on one side indicates the capillary size of 20 μL. The label
on the other side indicates the capillary size of 100 μL.
Equip the Rack LC. Only use the same size of capillaries for a single application.
Labware accessories
epMotion® 5073 59
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Abb. 8-4: Thermorack 0.5 mL; 1.5 mL; 2 mL Abb. 8-5: Rack 0.5 mL; 1.5 mL; 2 mL
Fig. 8-4: Thermorack 0.5 mL; 1.5 mL; 2 mL Fig. 8-5: Rack 0.5 mL; 1.5 mL; 2 mL
Thermoracks and the 0.5/1.5/2.0 mL Racks have 24 bores for vessels with a volume of 1.5 mL and 2 mL.
Vessels with a volume of 0.5 mL can be inserted with adapter sleeves.
Thermoracks and the 0.5/1.5/2.0 mL Racks have lid holders. The lid holders are on the right, next to the
bore for the vessel. The lid holder vertically clamps the tube lids.
Thermoracks and the 0.5/1.5/2.0 mL Racks are transported using the gripper.
Thermorack TMX
The Thermorack TMX is designed for use with the ThermoMixer. The Thermorack TMX is lighter than a
thermorack and provides for higher speeds during mixing. The Thermorack TMX has a low heat capacity
but a quick heat transfer. The Thermorack TMX quickly reaches the target temperature but cannot maintain
this temperature for a long time after temperature control.
The Thermorack TMX is available with installed adapters for vessels with a volume of 0.5 mL.
Tab. 8-1: Test values for temperature control of thermoracks and thermoadapters
Thermorack/thermoadapter Equipping Filling volume per Time taken to heat
vessel from 0 °C to 10 °C
Thermorack 1.5/2.0 mL 1.5 mL Safe-Lock tube 1000 μL ~ 30 min
Thermoadapter PCR 96 96-well twin.tec PCR plate 150 μL ~ 14 min
Thermoadapter PCR 384 384-well twin.tec PCR plate 25 μL ~ 10 min
Labware accessories
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8.1.5 PrepRack
Abb. 8-6: PrepRack
The PrepRack is specially designed for use with the magnetic finger module. You can also use the PrepRack
as the standard rack.
The PrepRack has 24 vessel bores which are numbered row by row. The corresponding lid holder is at the
right of the vessel bore. The lid holder vertically jams the vessel lid.
You can equip the PrepRack in the same way as the thermorack and the 0.5/1.5/2.0 mL Rack (see p. 59).
The PrepRack is designed to be used on the ThermoMixer The PrepRack is temperature-controlled and
designed to be used on thermal modules.
8.1.6 ReagentRack
Abb. 8-7: ReagentRack
The ReagentRack holds a tray with reagents from the Eppendorf MagSep kits.
The ReagentRack cannot be placed on the TMX.
The optical sensor detects the tray with reagents using its code.
8.1.7 ReservoirRack
Abb. 8-8: Equipped ReservoirRack
1 2
The ReservoirRack (4) is used for holding Reservoirs (1) and ReservoirRack modules (2). The
ReservoirRack columns are numbered (3).
The ReservoirRack can hold a maximum of 7 Reservoirs (1) or 7 different ReservoirRack modules (2). The
ReservoirRack may be equipped in any order.
The ReservoirRack modules in the outer positions are more difficult to temper.
Insert the ReservoirRack modules and Reservoirs in the ReservoirRack with the code facing the rear.
ReservoirRack modules are equipped with vessels and inserted into a ReservoirRack. All of the vessels
equipped in a ReservoirRack module must be of the same type.
The 30 mL TC ReservoirRack module and the 100 mL TC ReservoirRack module must be fastened to the
next ReservoirRack module using 2 connecting bars.
Insert the 2 connecting bars for the TC ReservoirRack modules in the next ReservoirRack modules.
3
2
The ReservoirRack modules have bores of 12 mm, 16 mm and 17 mm in diameter at the front and rear. The
bores are mounted at 50 mm, 60 mm, 70 mm, 80 mm and 90 mm. Use the 2 positioning screws to position
the vessels at these heights.
Screw the 2 positioning screws into the bores at the required height.
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Tab. 8-2: Temperature control duration of ReservoirRack modules and reaction vessels
Temperature change Temperature change
from 23°C to 4°C from 23°C to 37°C
ReservoirRack Tube Set Temperature Set Temperature
module TC temperature control time temperature control time
RR module TC Ø 12 mm Tube Ø 12 mm 3 °C
RR module TC Ø 16 mm Tube Ø 16 mm 3 °C Approx. 30 m Approx. 17 m
38 °C
RR module TC Tube 5 mL 3 °C in in
Eppendorf tubes 5 mL
RR module TC Ø 17 mm Conical tube 15 mL 2 °C
Approx. 39 m Approx. 23 m
3 °C 39 °C
RR module TC Ø 29 mm Conical tube 50 mL in in
Approx. 46 m Approx. 28 m
RR module TC Reservoir 100 mL 1°C 40 °C
in in
for 100 mL Reservoir
The table shows the time it takes to reach the set temperature with specific Reservoir Rack modules and
tubes, if the user has set the set temperature in the software.
Labware accessories
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8.2 Adapter
The height adapters are used to offset the various heights of the labware. The tool holder does not need to
offset the height differences of the labware and remains at one height. The shorter paths of this tool holder
allow the application to run more quickly.
The height of the adapter is specified on the adapter. The combined height of the labware and the adapter
may not exceed 126 mm.
8.2.2 Thermoadapter
Abb. 8-13: Thermoadapter DWP 96
Thermoadapters are adapters for plates that can be tempered. A thermoadapter can be tempered on the
thermal module. Thermoadapters can be placed on the worktable with or without plates.
Thermoadapters will not be transported using the gripper. The gripper can remove a plate from the
thermoadapter or place it on the thermoadapter.
The Thermoadapter LC Samples is a tube holder for automatically filling MagNA Pure LC Sample
Cartridges. Thermoadapters and cartridges are a fixed combination for the epMotion.
The Thermoadapter Frosty is a modified height adapter which includes a PCR-Cooler. A PCR plate is placed
on the Thermoadapter Frosty. The PCR-Cooler cools the samples in this plate. The Thermoadapter Frosty
can only be used with skirted PCR plates.
1. Cool the PCR-Cooler by placing the PCR-Cooler in the lab refrigerator with the upper side down.
2. Insert the PCR-Cooler in the Thermoadapter Frosty.
3. Place a PCR plate on the Thermoadapter Frosty.
The PCR-Cooler changes color if a temperature of 7 °C is exceeded. The color changes from
purple to pink or from dark blue to light blue. The color value in the recesses of the
PCR-Cooler is a decisive factor for cooling samples.
Labware accessories
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8.3 Thermoblock
Abb. 8-16: PCR thermoblock 384 Abb. 8-17: PCR thermoblock 96
A thermoblock consists of a block and a plate. The plate is firmly connected to the block.
Thermoblock Plate firmly connected to the Filling volume per Time taken to heat from
thermoblock vessel 0°C to 10°C
PCR 96 96-well twin.tec PCR plate 150 μL ~ 14 min
PCR 384 384-well twin.tec PCR plate 25 μL ~ 10 min
Fig. 8-18: Tube lid rotated 45° to the surface of the thermoblock
Arrange the vessels in columns. Leave every other column open for the tube lid.
A maximum of 48 vessels can be positioned with the lid in the thermoblock.
Labware accessories
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The waste box can hold up to 300 pipette tips of 1000 μL in size.
Prerequisites
• Disposal bag
• Clamping ring
1. Place the disposal bag in the waste box.
2. Fasten the disposal bag with the clamping ring.
3. Tightly pull the edge of the disposal bag downward.
Labware accessories
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1
2
3
The LiquidWasteTub is a container for liquid waste and is hung in the waste container.The LiquidWasteTub
has a working volume of 115 mL.
The optical sensor detects if the LiquidWasteTub is present. The optical sensor also detects the filling level
of the LiquidWasteTub.
The lid of the LiquidWasteTub prevents contamination due to splashes from the liquid waste. The lid only
can be used with single-channel dispensing tools. If you work with multi-channel dispensing tools, remove
the lid from the LiquidWasteTub.
The LiquidWasteTub is reusable. You can decontaminate and clean the LiquidWasteTub (see
Decontamination and cleaning on p. 85).
You have to define in the software that the waste container is equipped with the LiquidWasteTub.
Labware accessories
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9 Operation
9.1 First steps
This chapter describes how to operate the epMotion and carry out the applications.
Prerequisites
• The epMotion has been installed and commissioned by skilled specialists authorized by Eppendorf.
1. Close the front hood.
2. Switch on the device using the mains/power switch.
• EasyCon Tablet starts and displays the start screen.
• The carrier travels to the home position.
• The epMotion is ready for operation.
The software manual describes in detail how to use the EasyCon Tablet.
9.1.2.2 Mouse
Connect the USB mouse to one of the USB ports of the EasyCon Tablet.
The mouse arrow appears on the screen as soon as the mouse has been recognized.
Operation
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NOTICE! Data loss due to missing data back-up or incorrect storage of data carriers.
User accounts, applications, labware and logs are stored in one database. If this database gets
damaged (e.g. due to a hardware defect), the information is lost.
Eppendorf is not liable for a loss of data and any resulting damage.
Save the database regularly with the Backup function in the software.
Save stored data on external data carriers.
Store the data carriers in accordance with the manufacturer's instructions.
In order to transfer or back up data from the EasyCon Tablet, you need a USB storage medium.
Step 1 is described in the software manual. Steps 2 to 4 are described in the following sections.
Operation
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In order to prepare the epMotion for an application, perform the following steps:
• Displaying the equipping in the software
• Positioning tools
• Placing epT.I.P.S. Motion
• Placing the waste container
• Positioning labware
50
M5073
T1 T2 A1 A2
G1 B1 B2
C1 C2
P5073
The epBlue worktable displays the equipping of the epMotion worktable in a graph.
3. Equip the epMotion worktable as displayed.
Details on equipping can be found in the following chapters.
Operation
76 epMotion® 5073
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When the application is started, epMotion checks if the required tools are available. The order of the
dispensing tools can be user-defined.
Place the dispensing tool with the gold contact to the right rear onto the holders T1 to T2.
9.2.2.2 Gripper
1 2
1
2
4 3
Fig. 9-2: Open (1) and close (2) the gripper arms.
Observe the instructions for use for the epT.I.P.S. Motion Racks and epT.I.P.S. Motion Reloads.
The optical sensor detects the type of pipette tips using the coding on the tray. The epMotion removes the
pipette tips from the Rack column by column. Additional information on the epT.I.P.S is available (see p. 51)
1. When using epT.I.P.S. Motion Reloads, place the tray on the TipHolder.
2. Place the Rack or TipHolder on the worktable location with the label pointing toward the front.
3. Remove the lid.
Operation
78 epMotion® 5073
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Particularities on the placement can be found in the chapter on labware (see Labware accessories on p. 57).
9.2.5.1 Tubes
The labware must not have any play at the epMotion worktable location.
Details on starting and controlling applications can be found in the software manual.
If you are executing an application for the first time, carry out a test run.
Carry out the test run with a liquid having similar physical properties as the liquid to be used
in the application.
1. Fill the labware with the correct dispensing quantity of demineralized water or a test liquid.
2. Equip the epMotion worktable with labware and tools.
3. Activate the optical sensor.
4. Fully execute the application.
5. Check to see if the application was executed without errors.
• The dispensing is precise and correct.
• No liquid sprays out of the reaction vessels. Contamination is unlikely.
Additional information on optimizing the dispensing can be found in the software manual.
Operation
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Prerequisites
• Application is selected.
• Worktable is equipped (see p. 75).
1. Start the application.
2. Select the optical sensor settings for this run.
A detailed description of the optical sensor and its functions can be found in the software manual.
3. For applications with a variable sample quantity, enter the number of samples.
4. If the level measurement is deactivated, enter the filling volume of the vessels using the unit μL.
• The epMotion checks if the required tools are available.
• If necessary, the optical sensor checks the labware and filling levels.
• The application is executed. The software displays the status of the application.
Operation
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To stop the application immediately, e.g. in case of risk of collision, proceed as follows:
Stop the application with the button (refer to the epMotion software manual).
The current movement will be ended. Then the application stops.
You can continue or abort the application.
Prerequisites
• The equipping of the worktable has not been changed.
• The filling levels of the vessels have not been changed.
• The carrier has not been moved manually.
1. Close the front hood.
2. Continue the application with the button (refer to the epMotion software manual).
Operation
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Prerequisites
• The application was stopped or interrupted.
Abort the application with the button (refer to the epMotion software manual).
• The application will be aborted.
• The tool holder returns the tool to its original location.
• The carrier travels to the home position.
• The epMotion is ready for operation.
WARNING! Danger due to flammable and infectious liquids in the waste container.
Pipette tips in the waste container may contain flammable or infectious liquids.
Wear personal protective equipment.
Handle the pipette tips and sample materials from the waste container in accordance with
the material safety data sheets, safety regulations and laboratory guidelines.
10 Maintenance
10.1 Decontamination and cleaning
WARNING! Danger due to flammable and infectious liquids in the waste container.
Pipette tips in the waste container may contain flammable or infectious liquids.
Wear personal protective equipment.
Handle the pipette tips and sample materials from the waste container in accordance with
the material safety data sheets, safety regulations and laboratory guidelines.
Aids
• Decontamination agent
• Deionized water
• Lint-free cloths
• Cotton swabs
Prerequisites
• The device is switched off and disconnected from the mains/power line.
• The ejector sleeve of the single-channel dispensing tool has been removed.
decontamination agent.
2. Clean the surfaces with the lint-free cloth. Clean
hard to access surfaces with cotton swabs.
The surfaces are moistened with decontamination
agents.
3. Allow the decontamination agent to take effect.
4. Wipe the decontamination agent with deionized
water.
5. Allow the surfaces to dry.
NOTICE! Damage to device from the ingress of liquid during spray decontamination.
Do not clean the epMotion by means of spray decontamination.
Remove the labware and tools from the device before cleaning the labware and tools using
spray decontamination.
Aids
• Decontamination agent in spray bottle
• Deionized water
1. Remove the labware and the tools from the device.
2. Spray decontamination agents on the labware and tools.
3. Allow the decontamination agent to take effect.
4. Wipe the decontamination agent with deionized water.
5. Let the labware and tools dry.
Prepare the device and carry out the irradiation as described (see UV lamp on p. 35).
10.1.4 Autoclaving
Prerequisites
• 121 °C at 100 kPa overpressure are set on the autoclave.
• Any chemical decontamination agents have been removed.
1. Autoclave the tools and labware for at least 20 min.
2. Rinse the tools and labware with deionized water.
3. Let the tools and labware dry.
Maintenance
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The Eppendorf AG service team is available to service and certify your device.
10.4 Fuses
Fuses may only be replaced by authorized service technicians. Users must not replace the fuses.
Maintenance
90 epMotion® 5073
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Prerequisites
• The firmware is located on a USB storage medium.
• The device is switched off and disconnected from the mains/power line.
1. Connect the USB storage medium to a USB port on the rear of the epMotion.
2. Switch on the epMotion.
The epMotion automatically installs the firmware.
3. Wait until the epMotion has emitted 3 short signal tones.
4. Exit epBlue (refer to the epMotion software manual).
The EasyCon Tablet shuts down. The display becomes dark.
5. Switch off epMotion.
6. Remove the USB storage medium.
Maintenance
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When you use liquids with a density greatly different from that of water, the dispensing volume may be
incorrect. Physical properties, such as the viscosity, vapor pressure and surface tension of the liquid, affect
the dispensing volume.
For these liquids, correct the dispensing volume. Gravimetrically check the dispensing of the epMotion and
correct the dispensing if necessary.
Changing the density by 10% will change the dispensing result by approx. 0.2%-1%.
Exchange the sealing rings at the described intervals and if the following problems occur:
• Sealing rings are damaged.
• Tips are not attached in parallel.
• Tips are dripping.
• Once a year.
Prerequisites
• New sealing rings
• Auxiliary tool
• Mounting aid
• Damp, lint-free cloth
1. Place the edge of the auxiliary tool at the height of the sealing ring.
2. Use the auxiliary tool to cut the sealing rings on the dispensing tool.
3. Remove the sealing rings with the fingers.
4. Clean all tip cones with a damp, lint-free cloth.
5. Attach the new sealing ring with the mounting aid.
6. Position the sealing rings in the grooves of the tip cones.
Maintenance
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Customer service functions are available to service and check your device.
11 Troubleshooting
If you cannot remedy an error with the recommended measures, please contact your local Eppendorf
partner. The contact address can be found online at: www.eppendorf.com.
Device
• Is the front hood of the epMotion or the safety glass of the cleanbench completely closed?
Tools
• Are the required dispensing tools available?
• Is the gripper available?
• Have the tools been correctly hung in the holder?
Worktable
• Is the labware displayed in the software identical to the labware on the epMotion worktable?
• Is the labware at the location shown on the display?
• Are all locations which are shown as empty in the software actually empty on the epMotion worktable?
Labware
• Is the labware correctly inserted?
• Is the labware inserted laterally reversed?
• Do you use the correct height adapter?
• Are all vessels open?
• Are the epT.I.P.S. Motion Racks sufficiently filled with pipette tips?
• Have the lids of the epT.I.P.S. Motion Racks been removed?
• Are the lids of the Safe-Lock tubes positioned correctly?
• Are the required filling quantities in the source positions?
• Has the volume of the Racks or plates, which will be required later, been entered in the application?
Waste container
• Is the waste container empty?
• Is the disposal bag inserted correctly in the waste container?
• Is the clamping ring flush?
• Does the bag protrude over locations B1 or A1?
• Is the bag able to hold the quantities of tips required for the application?
Troubleshooting
96 epMotion® 5073
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Use the yellow carrying straps to lift the device out of the packing and position it.
12.2 Storage
12.3 Disposal
In case the product is to be disposed of, the relevant legal regulations are to be observed.
Information on the disposal of electrical and electronic devices in the European Community:
Within the European Community, the disposal of electrical devices is regulated by national regulations
based on EU Directive 2012/19/EU pertaining to waste electrical and electronic equipment (WEEE).
According to these regulations, any devices supplied after August 13, 2005, in the business-to-business
sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. To
document this, they have been marked with the following identification:
Because disposal regulations may differ from one country to another within the EU, please contact your
supplier if necessary.
Technical data
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13 Technical data
13.1 Power supply
13.3 Weight/dimensions
13.5 Interfaces
Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected
to the interfaces.
13.7.2 Carrier
13.7.3 Gripper
13.8 Enhancements
13.8.1 Thermal module
13.8.2 ThermoMixer
13.8.5 UV lamp
13.9.2 Dispensing
Test conditions and test evaluation in accordance with ISO 8655, part 6. Test with analytical balance with
evaporation protection, approved by the board of weights and measures.
14 Ordering information
14.1 Tools
14.2.4 Adapter
14.3 Plates
14.4 Reservoirs
14.5 Racks
Order no. Description
(International)
Racks for individual tubes
for use with Eppendorf vessels and glass or plastic test tubes, cannot be tempered
5075 761.009 Ø 17 mm × 100 mm max. length
5075 775.000 Ø 17 mm × 60 mm max. length
5075 760.002 Ø 16 mm × 100 mm max. length
5075 776.006 Ø 16 mm × 60 mm max. length
5075 792.028 Ø 15 mm × 100 mm max. length
5075 792.044 Ø 15 mm × 60 mm max. length
5075 792.001 Ø 14 mm × 100 mm max. length
5075 792.060 Ø 14 mm × 60 mm max. length
5075 762.005 Ø 13 mm × 100 mm max. length
5075 792.087 Ø 13 mm × 60 mm max. length
5075 763.001 Ø 12 mm × 100 mm max. length
5075 792.109 Ø 12 mm × 60 mm max. length
Rack
for 24 Safe-Lock tubes
5075 751.453 0.5 mL/1.5 mL/2.0 mL, cannot be tempered, with adapter sleeves für 0.5 mL
5075 751.275 1.5 mL/2.0 mL, cannot be tempered
Rack
for 24 HPLC vessels
5075 792.125 Ø 12 mm × 40 mm max. length
Rack
5075 791.005 for 96 × 1.5/2.0 mL conical tubes
Rack Smart
5075 790.009
Rack LC
5075 795.000 20 μL/100 μL
ReservoirRack Module TC
for
5075 799.049 PCR 0.2 mL
5075 799.065 PCR 0.5 mL
5075 799.081 Safe-Lock tube 1.5 mL/2 mL
5075 799.103 vessels Ø 12 mm
5075 799.120 vessels Ø 16 mm
5075 799.162 vessels Ø 17 mm
5075 799.189 vessels Ø 29 mm
5075 799.146 Reservoir 30 mL
5075 799.260 Reservoir 100 mL
5075 799.340 Eppendorf Tubes 5.0 mL
ReservoirRack
5075 754.002 for 30 mL and 100 mL reservoirs and ReservoirRack Module TC
ReservoirRack 3
5075 754.070 for 30 mL and 100 mL reservoirs
PrepRack
for 24 Safe-Lock tubes 2 mL
5073 751.006 for nucleic acid preparation with MagSep kits
Ordering information
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14.6 Adapter
14.7 Thermoblocks
14.9 Accessories
Modification kits and enhancements may only be mounted by authorized service technicians.
P 30 mL Reservoir .............................................. 50
400 mL Reservoir ............................................ 50
PCR..........................................................................8
Ordering information..................................... 109
PCR plate ...............................................................49
ReservoirRack module
PDF..........................................................................8 Placement ........................................................ 78
Pipette tips
epT.I.P.S. Motion ..............................................51 S
epT.I.P.S. Motion Rack .....................................52
Safety equipment ............................................ 23, 24
epT.I.P.S. Motion Reload..................................54
epT.I.P.S. Motion SafeRack ..............................53 Service
Multiple use .....................................................53 Dispensing tool................................................ 91
Placement ........................................................77 Dispensing tool sealing rings .......................... 92
TipHolder .........................................................55 Service provisions ........................................... 89
Pipetting ................................................................42 Spray decontamination ......................................... 88
Placement Storage.................................................................. 99
Adapter.............................................................79
Dispensing tool ................................................76
T
Gripper .............................................................76
Plate .................................................................79 Technical data
Rack .................................................................79 Ambient conditions ....................................... 101
ReservoirRack module .....................................78 Carrier ........................................................... 102
Tube .................................................................78 EasyCon Tablet .............................................. 102
Waste container ...............................................78 Gripper .......................................................... 102
HEPA filter ..................................................... 103
Plate.......................................................................48
Magnetic finger module ................................ 103
Deepwell plate .................................................48
Noise level ..................................................... 101
Microplate ........................................................49
Optical sensor................................................ 102
Ordering information .....................................109
Thermal module ............................................ 103
PCR plate..........................................................49
ThermoMixer................................................. 103
Placement ........................................................79
UV lamp......................................................... 104
Test run ................................................................. 80
R
Thermal module.................................................... 29
Rack
Decontamination and cleaning........................ 86
0.5/1.5/2.0 mL Rack .........................................59
Equipping ........................................................ 31
Ordering information .....................................110
Properties ........................................................ 18
Placement ........................................................79
Technical data................................................ 103
PrepRack ..........................................................60
Rack for 24 vessels...........................................57 Thermoadapter
Rack for 96 vessels...........................................57 Thermoadapter Frosty ..................................... 67
Rack LC ............................................................58 Thermoadapter LC Samples ............................ 66
ReagentRack ....................................................60
Thermoblock ......................................................... 68
ReservoirRack ..................................................61
Equipping ........................................................ 68
ReservoirRack module .....................................62
Ordering information..................................... 111
Remaining volume.................................................40
Reservoir
100 mL Reservoir.............................................50
Index
116 epMotion® 5073
English (EN)
U
USB .........................................................................8
UV............................................................................8
UV irradiation........................................................88
UV lamp.................................................................35
Technical data ................................................104
W
Waste box ..............................................................69
Waste container
LiquidWasteTub ...............................................70
Waste box.........................................................69
Wipe decontamination ..........................................87
Workflow ...............................................................74
Worktable
Cleaning up ......................................................84
Decontamination and cleaning ........................85
Display of equipping .......................................75
Equipping ........................................................75
Gripper placement ...........................................76
Locations..........................................................27
Placing dispensing tools ..................................76
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