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CCBC, Spring 2021 School of Mathematics and Science

Biology Department, Dundalk Campus
BIOL 230: Microbiology Section(s) DR1 CRN#s: 22331
Course Description and Prerequisites1:

Introduces and discusses concepts related to the study of bacteria, viruses, protozoa, fungi and both the
beneficial and pathogenic interrelationships with humans; emphasizes basic laboratory techniques such
as microscopy, staining, and aseptic technique. This course is often required for allied health pre-nurs-
ing programs and may be taken by prospective Biology major transfer students.

3 lecture and 3 laboratory hours per week.

Prerequisites: Completion of BIOL 110 with a final grade of "C" or higher and (ENGL 052 or ESOL
052 or LVE 2 or LVE 3 or LVE 4) and (MATH 082 or MATH 013 or LVM2 or LVM3 or LVM 4 or
LVM5)...

I. Basic Course Information

A. Instructor: Dr. Kelly Basi
B. Office: Building MASH: 211
Contact Information: Phone: 443-840-3968 E-mail: [email protected]
C. Office Hours: Monday (Virtual)- 8:45 PM -9:45 PM and Friday (Virtual) – 5:00 PM – 5:45 PM
via Microsoft Teams ; Or by appointment
D. Department/School Phone Number: Dundalk Science Department: 443-840-3292.
E. Class Times, Days, and Locations:

Lecture: Synchronous Virtual (Microsoft Teams) - Monday 5:45 PM-8:45 PM

Lab: Face-to-Face - MASH 210 – Wednesday 5:45 PM-8:45 PM

F. Statement of Student Out-of-Class Work Expectations. These expectations are the minimal
requirements based on national standards and may not be decreased: in a standard 16 week
semester, students are expected to spend 2 hours per week, per credit hour on work outside of class.
This is a 4 credit course taught in a 14-week semester, and so you are expected to complete at least
(8) hours of work per week outside of the class including reading, class preparation, homework,
studying, etc If this is an online section, an additional 4 hours are required per week. Students:
please note that these are minimal requirements for any course, and that many students require more
time than this for science courses.
G. Materials:
Required: For laboratory courses, appropriate clothing (including shoes which cover the tops of
the feet and have good traction) is required. See “Course Procedures” for more information:
Required Textbooks:
 Foundations in Microbiology, 10th Edition .Talaro:McGraw Hill Publishers.
 BIOL 230 Microbiology Lab Mannual, Biology Department, Essex and Dundalk by
Gilinger, Trueba, Madike, Laing and Moure
 Lab Coat and Goggles

OPTIONAL Texts and resources:

 Microbiology: A Photographic Atlas for the Laboratory. Alexander and Strete; Addison Wesley Pub-
lishers.
 Microbiology Laboratory Theory and Application Brief Edition.Leboffe and Pierce: Mor-
ton Publishing.
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II. Course Goals Overall

A. Course Objectives: Upon completion of this course the student will be able to:

1. classify organisms using appropriate terminology related to their structure, metabolism, genetics, and
ecology;
2. explain the functions of key microbial structures in the prokaryote cell and their contribution to disease
development;
3. explain the differences among Gram negative cell walls, Gram positive cell walls, and atypical cell
walls, and their effects on differential staining in the Gram stain;
4. differentiate the metabolic processes of aerobic respiration, anaerobic respiration, and fermentation;
5. differentiate gene regulation through quorum sensing and gene regulation via an operon;
6. delineate the processes of the three different types of horizontal gene transfer and the different mecha-
nisms that allow bacteria to generate diversity and acquire resistance elements;
7. discuss the differences between opportunistic and pathogenic microorganisms and host-pathogen inter-
actions that result in infection and/or disease;
8. use proper terminology to describe various types of infectious disease, the stages of a disease, and means
of transmission;
9. describe the general steps in a productive and a latent viral replication cycle using proper terminology to
identify key viral structures in those cycles;
10. explain innate immune responses such as phagocytosis, complement, and inflammation as they apply to
the infectious disease process;
11. explain which cells and cytokines are involved in adaptive responses and how their interactions lead to a
primary response;
12. describe the concept of memory and how it allows for the acquisition of immunity, both natural and arti-
ficial;
13. explain the principles of certain physical and chemical methods used in the control of microorganisms;
14. describe the modes of action that enable antibiotics to inhibit or kill bacteria, as well as the common
mechanisms that enable bacteria to resist these antibiotics;
15. demonstrate appropriate laboratory skills and techniques related to the isolation and staining of micro-
organisms;
16. demonstrate proper use and care of the compound light microscope;
17. identify an unknown microorganism using phenotypic methods; and
18. develop the ability to work both independently and with others in the laboratory and draw appropriate
conclusions from laboratory results.

B. Major Topics
I. Introduction to microbiology
II. Prokaryote cell anatomy
III. Classification of microorganisms: bacteria, viruses, protozoans, and fungi
IV. Microbial growth and reproduction
V. Microbial metabolism
VI. Microbial genetics
VII. Microbial mechanisms of pathogenicity
VIII. Innate and adaptive immune responses
IX. Control of microorganisms
X. Microorganisms and human diseases
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XI. Laboratory techniques

a. Microscopy
b. Aseptic technique
c. Staining of microorganisms
d. Identification of bacteria and viruses
e. Control of Microorganisms
C. Rationale: The rationale of the course is to meet the needs of students who are interested in the biology of
microorganisms and the laboratory skills needed to culture, manipulate and control bacteria; or who may choose
an allied health field; may wish to pursue a biology, physical education, zoology, pre-medical or nursing major;
or are actively engaged in an allied health field and which to reinforce or update their knowledge. This course
contains a laboratory component. This course is a Science core course, Science elective, and an Arts and
Sciences elective.

III. Evaluation
A. Requirements1: This course consists of fourteen lecture chapter quizzes, four lecture exams, and
two lab exams. To successfully complete the course students will be required to take all quizzes,
exams in both lecture and lab on the scheduled dates.

Lecture Points:
3 Lecture Exams (100 points each) 300 points
Final Exam 150 points
Lecture chapter quizzes (10, 5 points each on Blackboard) 50 points
Interactive Participation (10 points each) 40 points
2 Case Studies (2, 50 points each) 100 points

Total Lecture Points: 640 Points

Lab Points:
Morphological Unknown Lab Identifications 50 points
2 Identification of Unknown bacteria (30pts each) 60 points
2 Practical Exams (75pts each) 150 points
Pre-lab Weekly lab quizzes 10 points each (Blackboard) 50 points

Total Lab Points: 310 Points

Total Lecture/Lab Points: 950 Points
TOTAL POINTS: 870 Points
A = (90-100%)
B = (80-89%)
C = (70-79%)
D = (60-69%)
F = (59% and lower)

i. Extra Credit: There may be extra credit assignments for a total of 30 pts

IV. Course Procedures

A. Course-related policies and procedures

Instructor's grading policy: Course evaluation procedure is based upon the premise that a student's regular
attendance and ability to answer test and practical examination questions is an approximation of that student's
mastery of the specific course objectives. Examination questions are therefore designed to objectively measure
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both the scope and depth of the student's mastery. The instructor will assign letter grades based on the
following calculation:

Attendance: You are expected to attend all lecture classes and labs regularly. You are also responsible for
materials covered during your absences. Instructors may be willing to consult with you for make-up
assignments, but it is your responsibility to contact the instructor. Class attendance is monitored daily.

In addition, Roll will be taken but attendance is your responsibility. I expect students to attend all classes.
Good class attendance is absolutely essential to do well in this course! It is a good idea to exchange names
and phone numbers with others in the class so that you can obtain the information you missed as quickly as
possible. In case of a prolonged absence (2 or more class meetings), the instructor should be notified as soon as
possible.

Assignments:
Reading homework may be assigned. You are expected to read and take notes and be prepared to participate in
class discussions. Read the PowerPoints before coming to class! It will make learning a lot easier! Work is
due on the assigned day. Late work will not be accepted.
Lecture Exams/Quizzes:

All lecture exams/quizzes will consist of questions that may include multiple choice, fill-in-the-blank, True-
False, matching, and/or short answer questions.

All lecture chapter quizzes will be online (Blackboard) and you have 30 minutes to complete it. Only two
attempts will be allowed and the higher of the two grades stays in your gradebook. So take the quiz once you
have read the chapter. Quizzes must be completed on the due date, no extensions will be given. Since there are
no make-up’s for quizzes, you will be given a zero for that quiz.

Lab Exams/Quizzes:

All lab quizzes will be given at the beginning of lab and should take approximately 15 minutes. If you come
into lab late, you will only be given the remaining amount of time to finish the quiz. If you come into class
after the last quiz has been picked up, you will not be given a quiz and since there are no make-up’s for quizzes,
you will be given a zero for that quiz. Bottom line - DON’T BE LATE TO A QUIZ!!!!

All lab exams/quizzes will consist of questions that may include multiple choice, fill-in-the-blank, True-False,
matching, and/or short answer questions.

Exam and Quiz policy: 1. Exams will be given at the beginning of class time and/or a deadline will be set. All
Lecture exams will be administered through Blackboard and requires Respondus Lockdown Browser. This
means that your computer must have a camera. You will have 100 minutes to complete the exam.

Make-up Exams/Quizzes:
No make-up exams or quizzes will be given unless the instructor is notified in advance and only for emergency
situations approved by the instructor. Students will be required to submit a doctor’s or hospital note to the
instructor before a make-up exam will be administered. Under no circumstances will retests be administered.
Participation in all laboratories is mandatory. No Make-up labs will be given. If you are going to miss a lab,
you need to contact your instructor to see if space is available in another lab section during the SAME week.
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Allowing you to attend a different laboratory section is up to the discretion of that lab’s instructor and
APPROVAL IS NOT GUARENTEED! You will still be held responsible for any & all material that you
missed.

IN CLASS TECHNOLOGY (Cell Phones, Laptops, etc.):

All phones must be turned off or be in silent (courtesy) mode. Please do not text or access you phone during
class time or in the laboratory. You may not leave the room during a test to answer a phone call. Should this
occur your test will be taken up immediately. I will allow the use of laptops in class, but only if you are using
them for note taking purposes. The use of computers in class for email, facebook or any other purpose beyond
appropriate course work is strictly prohibited.

INSTRUCTORS’S ASSURANCE:

Instructor will return all email correspondence (if you email me, please include your name and what class you
are in) within a 24-hour period Monday through Friday. All exams and quizzes given in lecture or lab will be
graded and scores given within a week of the exam date. Instructor will provide the students with the
opportunity to review and discuss their exams.

BEHAVIOR AND DECORUM:

Unless the students are asked by the instructor to conduct discussions, talking is not allowed in lectures or labs.
Communication among students during lab for group work is allowed with noise level kept to a low level. The
instructor will ask a student who disturbs lectures/labs to leave the room, and be marked “absent” for the day.

QUESTIONS/PROBLEMS:

Please make sure that if you have any questions or problems at any time, that you first contact me as soon as
possible. The worst thing you can do is wait to contact me or to not take advantage of the resources available to
you. By taking an active part in your education, you will make your academic experience much more
rewarding and exciting!!

B. College wide syllabus policies: For college wide syllabus policies such as the Code of Conduct related to
Academic Integrity and Classroom Behavior or the Audit/ Withdrawal policy, please go to the
‘MySyllabiPolicies’ Tab on the MyCCBC page. Please pay particular attention to the following sections of
MySyllabiPolicies:
 Attendance Policy
 Code of Conduct for Academic Integrity
Grades – AU (Last day to switch to an audit for this semester is: Friday – February 19th, 2021

 Grades -W (Last day to withdraw for this semester is: Wednesday– April 14th, 2021

C. Contact information for course-related concerns: See endnote2.

D. Additional Procedures: No food or drink is allowed in any science lab at any time. Feet should be covered
with closed-toed shoes to provide protection from broken glass, spilled chemicals, and other laboratory
hazards. Eye, hand, skin and clothing protection may be required when chemical or biological hazards are
present. Failure to abide by laboratory safety policies will result in removal of students from the class.

Add additional material as appropriate.

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E. Course calendar/schedule: See the printed schedule appended to this document. Optional links to
online calendars. (Modify/delete as appropriate.)

For all science lab courses, face shields will be provided to students and instructors due to the closer inter-
actions that takes place within the lab. 
 
All students and instructors within the lab must wear their personal face covering, and either a face shield
or goggles at all times during lab.  If the course requires microscope work, OSHA approved goggles (sold in
bookstore) will be required to be worn for this portion of the class rather than a face shield. 
 
Required PPE will include:
1.        Gloves (provided by college)
2.        Personal face covering (mask), 
3.        A face shield (provided by college) and/or OSHA approved goggles if microscope work is required
(Goggles must have a flat surface front for microscope use)
 
If students do not meet these PPE requirements they will not be permitted to enter the lab – no exceptions.

This syllabus may be changed with notification to the class.

Note that the content in these sections is dictated by the Common Course Outline for this course, as approved at the college-wide
level: [http://www.ccbcmd.edu/cco/home.html]
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Students should first attempt to take concerns to the faculty member. If students are unable to resolve course-related concerns with
the instructor, they should contact Dr. Robin Minor, Dundalk Science Coordinator, at [email protected] or 443-840-3756.
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Spring 2021 Lecture Schedule (M/W)

Date Chapter Text Book Reading Topic Quiz Dates
2/1 1 2-22 The Main Themes of Microbiology (Group #1)
4 90-118 Survey of Prokaryotic cells, microbes (Group #2)

2/8 8 Microbial Metabolism (Group #3) QUIZ #1 (1,4)

7 190-216 Microbial Nutrition and Growth (Group #4)
Group #1 and #2 present questions on CH 1 and 4

2/15 Review for Exam 1 (745-845PM) QUIZ #2 (8,7)

Group #3 and #4 present on questions on CH 8 and CH 7
Exam 1 (Chapters: 1, 4, 8 and 7) – Due by 11:59PM on 2/21

2/22 9 262-292 Microbial Genetics (Group #1)

3/1 10 300-319 Genetic Engineering (Group#2)

13 399-342 Microbe-Human interaction (Group #3/4) QUIZ #3 (9,10)

3/8 Case Study #1 is Due Review QUIZ #4 (13)

Group #1-#4 present on questions
Exam 2 (Chapters 9, 10, & 13) – Due by 11:59PM 3/14

3/15 5 126-155 Survey of Eukaryotic Cells (Group #1)

3/22 6 161-183 Introduction to Viruses (Group #2/3)

18 557-572 Medically Important Gram + Microbes (Group #4) QUIZ #5
Group #1 present on questions from CH5

SPRING BREAK – March 27 – April 6

4/12 20 619-632 Medically Important Gram -ve Microbes

Groups #2-4 present on questions from CH 6 and 18
Exam 3 (Chapters 5, 6, 18, & 20) – Due by 11:59PM 4/18

4/19 11 329-345 Chemical/Physical Control agents (Group #1)

12 361-391 Antibiotics (Group #2)

4/26 14 468-488 Innate Defenses (Group #3)

Groups #1 and 2 present on questions

5/3 15 468-495 Adaptive Immunity (Group #4) QUIZ #6, #7 (11,12)

QUIZ #8 (14)
Group #3 present on questions

5/10 16 503-527 Immune Disorders / Immunodeficiency QUIZ #9/10 (15/16)

Group #4 present on questions

5/12 Case Study #2 is DUE!

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5/17 Exam 4-Final (Chapters: 11, 12, 14, 15) – Due by11:59PM on Wednesday, 5/19

Note: The Lecture schedule is tentative and subject to change.

Microbiology Biol. 230 Spring 2021 Laboratory Schedule (M/W)

Week Session Experiment Title

1 (2/3) Lab 1 Lab Safety, Survey of Microbes
Lab 2 Introduction to Microscope

2 (2/10) Lab 3 Introduction Eukaryotes

Lab 4 Aseptic Transfer

3 (2/17) Lab 6 Intro to Bacterial Staining (Simple Staining).

Lab 7 Differential Staining: The Gram Stain

4 (2/24) Lab 8 Differential Staining: The Acid-Fast Stain

Lab 10 Structural Staining (Endospore Stain) / Make up

5 (3/3) Lab 10 Structural Staining (Negative and Capsule Stain)

Lab 5 Bacteria Isolation of Bacteria-Streak/spread Plate

6 (3/10) Morphological Unknown

Lab 11 Intro to Selective and Differential Plated Media

7 (3/17) Lab 20 Urinalysis Lab

Disinfectants, Antiseptics and Antimicrobial Agents / Review

8 (3/24) Lab 19 Interpret

Lab Exam 1 – In Class

9 (3/29-4/5) Spring Break-No Lab 3/29, 3/30, 4/1, & 4/5

10 (4/7) Hand Out Physical Control Methods: UV Radiation

Hand Out Microbes in Water: Multiple-Tube Technique

11 (4/14) Lab 22 Microbes in Food: Bacterial Count of Food

Lab 17 Isolation and ID of (GPC) Staphylococcus

12 (4/21) Lab 18, and 21 Isolation and ID of Gram-Positive Streptococcus

Labs 12, 13, and 14 Carbohydrate Fermentation; Protein Catabolism

13 (4/28) Interpretation, Transformation

Bacteria Unknown Project (GNR ID)

1 (5/5) Bacterial Identification Project

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15 (5/12) Review
Lab Practical 2 (Take Home)
Note: The lab schedule is tentative and subject to change or modification with notification to students.