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Worksheet 1

Advanced Cell and Molecular Biology

PART C. Use your codon chart to determine the amino acid sequence. Remember
to read through the strand and ONLY start on AUG and STOP when it tells you to
stop. Follow example below:

Example:

DNA  AGA CGG TAC CTC CGG TGG GTG CTT GTC TGT ATC CTT CTC AGT ATC

mRNA  UCU GCC AUG GAG GCC ACC CAC GAA CAG ACA UAG GAA GAG UCA UAG

protein  start - glu – ala –thre – hist – asp –glu – threo - stop

1. DNA  CCT CTT TAC ACA CGG AGG GTA CGC TAT TCT ATG ATT ACA CGG TTG
CGA TCC ATA ATC

mRNA 

protein 

2. DNA  AGA ACA TAA TAC CTC TTA ACA CTC TAA AGA CCA GCA CTC CGA TGA
ACT GGA GCA

mRNA 

protein 

3. DNA  TAC CTT GGG GAA TAT ACA CGC TGG CTT CGA TGA ATC CGT ACG GTA
CTC GCC ATC

mRNA 

protein 

4. DNA  TAA ACT CGG TAC CTA GCT TAG ATC TAA TTA CCC ATC

mRNA 

protein 

5. DNA  CTA TTA CGA TAC TAG AGC GAA TAG AAA CTT ATC ATC
mRNA 

protein 

6. DNA  TAC CTT AGT TAT CCA TTG ACT CGA ATT GTG CGC TTG CTG ATC

mRNA 

protein 

7. DNA  ACC CGA TAC CTC TCT TAT AGC ATT ACA AAC CTC CGA GCG

mRNA 

protein 

8. DNA  TAC AGA CGG CAA CTC TGG GTG CTT TGT TCT CTT CTC AGT ATC

mRNA 

protein 

Part D. Replication, Transcription & Translation Thinking Questions

1. Draw a DNA nucleotide & an RNA nucleotide. Label each of the 3 major parts.

2. What are the three major differences between DNA & RNA?

a)

b)

c)

3. What is the point of DNA replication? ____________________________

4. When & where does replication occur? _____________________________

5. What are three nucleotides together called on mRNA? _________


6. The mRNA codons can be used in a chart to find: ____________________

7. What molecule contains an anti-codon? _____________________________

8. Why is this (answer to #13) molecule so important?

9. Translation takes place in the ______________ on a ________________.

10. Transcription and translation together is the process of _______________________

11. What is any change in the DNA sequence called? ______________________________

12. What are some examples of mutations in gene expression?

13. If you replaced a base at the beginning of mRNA during transcription, what
would be the effect on the resultant protein? What if you removed a base entirely?

14. Are there instances when the DNA>RNA>Protein sequence of information is not
adhered to? What are these instances?

15. How does reverse transcription differ from transcription?

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