Galaxy 48 R/48 S CO Incubators: Operating Manual
Galaxy 48 R/48 S CO Incubators: Operating Manual
gnual
EN) S CO Incubators
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Copyright © 2014 Eppendorf AG, Germany. No part of this publication may be reproduced without the
prior permission of the copyright owner.
The company reserves the right to change information in this document without notice. Updates to
information in this document reflect our commitment to continuing product development and
improvement.
Trademarks
Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.
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Eppendorf has attempted to identify the ownership of all trademarks from public records. Any omissions or
errors are unintentional.
6705 900.025-00-/032014
Table of contents
Galaxy® 48 R/48 S CO2 Incubators 3
English (EN)
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Hazard symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Degrees of danger. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.1 Galaxy 48 R/48 S CO2 Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 48 R control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.3 48 S control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.4 Accessories provided . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.5 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.5.1 Operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.5.2 Direct heating system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.5.3 Infrared sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.5.4 Controlled humidity tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.5.5 Seamless chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.5.6 Multiple options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.6 Stacking devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.3 Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.3.1 Description of ATEX Guideline (94/9EC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.5.1 Personal injury and damage to device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.1 Utilities requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.3 Initial setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.3.1 Installing the feet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.3.2 Setting up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.3.6 Replacing the autozero filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5 Operation - Galaxy 48 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.1 Preparing for operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.2 Using the humidity tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.3 Programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.3.1 Programming CO2, O2 and temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.3.2 User access code. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.3.3 Removing user access code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.4 Referencing the CO2 sensor with autozero. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.5 USER settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Table of contents
4 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
6 Operation - Galaxy 48 S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
6.1 Preparing for operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
6.2 Using the humidity tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
6.3 Setting temperature and CO2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
6.4 Autozero system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.5 Programming the alarm system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.5.1 Setting the high and low temperature alarms. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.5.2 Setting the CO2 high and low alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.5.3 Door open alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.5.4 Alarm duration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Table of contents
Galaxy® 48 R/48 S CO2 Incubators 5
English (EN)
7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
7.1 O2 sensor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
8 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
8.1 Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
8.1.1 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
8.1.2 Daily Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
8.1.3 Weekly checks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
8.1.4 Monthly checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
8.1.5 CO2 Sampling with analyzer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
8.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
8.3 Disinfection/Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
8.4 High temperature disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
10 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.1 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.1.1 Equipment dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.1.2 Internal dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.1.3 Transporting dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.1.4 Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.2 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.2.1 Mains/electrical supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.3 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.4 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.4.1 Temperature management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.4.2 CO2 control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.4.3 Relative humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.4.4 Altitude limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
10.4.5 Storage temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
10.5 Oxygen sensor specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
10.6 CO2 recovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
11 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
11.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
11.2 Available options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Table of contents
6 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
13 Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Operating instructions
Galaxy® 48 R/48 S CO2 Incubators 7
English (EN)
1 Operating instructions
1.1 Using this manual
Carefully read this operating manual before using the device for the first time.
Also observe the operating manual enclosed with the accessories.
The operating manual should be considered as part of the product and stored in a location that is easily
accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our website
www.eppendorf.com.
Inhalation Crush
The following degree levels are used in safety messages throughout this manual. Acquaint yourself with
each item and the potential risk if you disregard the safety message.
Example Meaning
You are requested to perform an action.
1. Perform these actions in the sequence described.
2.
• List.
References useful information.
Product description
Galaxy® 48 R/48 S CO2 Incubators 9
English (EN)
2 Product description
2.1 Main illustration
2.1.1 Galaxy 48 R/48 S CO2 Incubators
Abb. 2-1: Front and back view of the 48 R CO2 Incubators
1 2 3 4 5 6 7
13 12 11 10 9 8
1 2 3 45 6 7
10 9 8
The control panel consists of an LCD display, 5 function keys and 4 direction keys.
The control panel consists of 2 individual 3-digit LED displays, and 4 function keys:
1 2
37.0
Temp °C
5.0
% CO2
enter
If you accidentally press both the up and down keys simultaneously, you will engage
Engineering Mode: press the Programming key immediately to exit.
Product description
Galaxy® 48 R/48 S CO2 Incubators 13
English (EN)
2.5 Features
The Galaxy 48 R/48 S CO2 incubator is microprocessor-controlled and designed to ensure accurate and
reliable operation.
The incubator incorporates a sophisticated control system that allows for easy programming, control and
monitoring of the chamber conditions.
A direct heating system, using a thermal heating element, completely surrounds the incubator, providing
an even temperature within the chamber. The independently and directly heated outer door is designed to
ensure an even distribution of heat. This system ensures a rapid, controlled return to optimum chamber
conditions after a door opening while also preventing any overshoot. The incubator’s direct heat system
provides for optimal use of laboratory space by allowing the most efficient internal volume for the footprint
of the instrument.
Product description
14 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
A solid-state infrared sensor is used to control the level of CO2 providing excellent reliability, while
remaining unaffected by humidity. The CO2 system has a semi automatic zero system (autozero) to
re-reference the sensor baseline to atmospheric CO2 levels at regular intervals. This provides for accurate
CO2 control.
An independently controlled water tray at the bottom of the incubator allows a high, uniform relative
humidity while preventing condensation in other parts of the chamber. Perforated shelves are provided as
standard to facilitate recovery of RH conditions in the chamber.
The 48 liter chamber is seamless, to provide a sanitary and easy-to-clean environment, and all internal
components are manufactured from polished stainless steel. The shelves (which are non-tip), shelf racks
and humidity tray are easily removed without tools for thorough cleaning and are capable of being
sterilized. Air circulation is achieved without the use of a fan, eliminating ductwork (a potential source of
contamination), simplifying cleaning, eliminating vibration, and facilitating use of microplates and
low-volume culture.
The Galaxy 48 R incubator features multiple options that can be installed to simplify maintenance and
provide superior control over experimental conditions. For example, high-temperature disinfection quickly
and conveniently disinfects the incubator’s chamber at 120 °C, without the need to remove interior
components or the CO2 sensor. A humidity tray warning system warns the user before the humidity tray
runs out of water, preventing dehydration of samples. The humidity tray must be emptied and the device
must be clean and dry prior to running high-temperature decontamination. Oxygen control provides for
conditions that require above- or below-ambient oxygen levels. These and other options and accessories
provide for a uniquely flexible CO2 incubator capable of meeting the most demanding requirements.
The incubator is not designed to be directly stackable. A second incubator may be safely stacked on top of
another identical incubator by using the custom-designed stacking stand available as an accessory. It is not
possible to put any other type of incubator or heavy apparatus on top, as the top cover and stacking stand
were not designed to support any other device.
Safety
Galaxy® 48 R/48 S CO2 Incubators 15
English (EN)
3 Safety
3.1 Intended use
Eppendorf CO2 Incubators are microprocessor-controlled instruments designed for cell culture. The
direct-heated, fanless chambers are designed to provide high humidity levels, minimal vibration and
precisely-regulated atmosphere of temperature and gas(ses) required for cell growth in T-flasks,
microplates, and other cultureware. They are intended for indoor laboratory use only.
The device may only be operated by trained lab personnel who have carefully read the operating manual
and are familiar with the device functions.
Do not operate the device in areas where work is completed with explosive substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which could create an explosive
atmosphere.
Due to its design and the ambient conditions in its interior, the device is not suitable for use in potentially
explosive atmospheres.
The device may only be used in a safe environment, e.g., the open atmosphere of a ventilated lab or fume
hood.
The use of substances which may contribute to a potentially explosive atmosphere is not permitted.
The final decision regarding the risks associated with using these types of substances is the user's
responsibility.
Safety
16 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
In the following cases, the designated protection of the device may be compromised.
The liability for the function of the device passes to the operator if:
• The device is not used in accordance with this operating manual.
• The device is used outside of the range of application described in the succeding chapters.
• The device is used with accessories or consumables that were not approved by Eppendorf.
• Service or maintenance is completed on the device by people who are not authorized by Eppendorf.
• The owner has made unauthorized modifications to the device.
Before using the device, read the operating manual and observe the following general safety instructions.
Elevated levels of CO2 may be found in and around the operating area of the CO2
incubator.
Wear personal protective equipment (PPE).
Do not touch the equipment during the high temperature disinfection cycle.
Do not open equipment door during the cycle.
Never try to lift the incubator by its door; this would cause permanent damage to the
incubator.
To avoid possible damage to the CO2 sensor, never leave water in the humidity tray while
the incubator is switched off, or when a high temperature disinfection cycle is initiated
(optional feature).
Allow a clearance of 15 – 20 cm (6 – 8 in) to allow access for oxygen sensor removal.
Safety
Galaxy® 48 R/48 S CO2 Incubators 17
English (EN)
CO2 gas pressure must not exceed 5 PSI (0.35 bar) unless operating with N2 or O2.
The instrument or equipment, and its external connections, to be used inside the chamber
should be specified as suitable for use in a humid environment, and at 37 °C (see also
“Using Powered Equipment within the Chamber”). If in doubt, consult with the
manufacturer of the equipment.
Always ensure the connections are properly and securely made.
Be sure to switch OFF the green illuminated switch on the front left of the IP66 enclosure
before connecting or disconnecting equipment inside the chamber.
The Sealing Cap must always be in place when the socket is not in use.
Both the incubator and the IP66 enclosure must be plugged into an electrical supply
protected by an RCD device. Any device chosen must be a self-resetting type which will
automatically reconnect power to the incubator as soon as power is restored following a
power failure.
Safety
18 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
Installation
Galaxy® 48 R/48 S CO2 Incubators 19
English (EN)
4 Installation
4.1 Utilities requirements
CO2 gas pressure must not exceed 5 PSI (0.35 bar) unless operating with N2 or O2.
Select a level surface capable of withstanding the operating weight of the incubator. Actual operating
weight will be dependent on both the options installed, and the material stored in the incubator.
The incubator is designed to operate at a chamber temperature of 4.0 °C above ambient, and at an absolute
minimum ambient temperature of 15 °C if the incubator is being operated at 37 °C.
Avoid placing the incubator in areas that may affect performance, such as those listed below.
To ensure adequate airflow for correct operation of the relative humidity control system the incubator feet
must be installed.
1. If they are not already installed, install the locking nuts onto each of the 4 feet provided.
2. Beginning with the front pair: tilt the incubator toward the back, and screw the feet in, to the required
depth.
3. Tilt the incubator forward to install the rear pair of feet.
4. Place an anti-slip pad (provided) on each foot.
4.3.2 Setting up
1. Install the power cord by inserting it into its receptacle on the back of the incubator and pressing it
firmly into its socket.
Connect the CO2 gas supply (see Connect the CO2 gas supply on p. 20).
Elevated levels of CO2 may be found in and around the operating area of the CO2
incubator.
Wear personal protective equipment (PPE).
1. Connect the incubator to the CO2 supply using the ~ 6 mm (1/4 in) plastic tubing (with installed filter) by
attaching the tubing from the 2-stage regulator (or in-line regulator) to the matching CO2 inlet on the
rear of the incubator.
It is highly recommended that an in-line regulator be used at the incubator’s gas inlet(s).
For proper incubator operation, CO2 gas pressure must not exceed 5 PSI (0.35 bar).
Installation
Galaxy® 48 R/48 S CO2 Incubators 21
English (EN)
4.3.4 Replacing the shelf racks and shelves, and level the incubator
The shelf racks and shelves are pre-installed. Perform the following steps if replacement is required.
1 Anti-tip tab
The humidity tray is pre-installed. Perform the following steps if replacement is required:
1
with its front and rear edges resting just above
the shelf rack supports.
1. Press the autozero filter gently into the filter socket at the top of the rear panel of the incubator.
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 23
English (EN)
5 Operation - Galaxy 48 R
5.1 Preparing for operation
1. Remove the black protective cover from the CO2 sensor, taking care not to remove the white porous
cover.
Store the black cover on the sensor cover holder on the back of the incubator. The sensor cap should be
placed back on the sensor when the incubator is to be cleaned.
2. Ensure that the white porous sensor cover remains in place.
3. Using the mains/power cord provided, connect the incubator to a earthed/grounded mains/power
supply.
4. Switch the incubator ON using the on/off switch at the rear of the cabinet.
The display will illuminate immediately.
5. Turn on the CO2 gas supply with the pressure regulator set to 5 PSI (0.35 bar) unless operating with N2
or O2.
6. The chamber setpoints are pre-programmed at 37.0 °C and 5 % CO2. Leave the incubator on until the
programmed chamber temperature and CO2 concentration have been reached.
• The incubator’s CO2 valve is disabled until the incubator reaches the temperature setpoint.
After the temperature setpoint is reached, the CO2 valve is activated, allowing the
incubator to reach the CO2 setpoint.
• If power is interrupted to the incubator long enough for the temperature to drop below
setpoint, the CO2 valve will be deactivated until temperature setpoint is again achieved.
(This serves to avoid spurious CO2 readings while the incubator is below its temperature
setpoint)
7. Leave the incubator running for at least two hours (preferably overnight) to allow conditions to stabilize.
To avoid possible damage to the CO2 sensor, never leave water in the humidity tray while
the incubator is switched off, or when a high temperature disinfection cycle is initiated
(optional feature).
Allow a clearance of 15 – 20 cm (6 – 8 in) to allow access for oxygen sensor removal.
If humidification is required:
1. Fill the humidity tray with 0.5 liters of warm (~37.0 °C) distilled water.
2. For cell culture work, we recommend adding a very small amount of copper sulphate in the humidity
tray. Tests have shown that, in addition to inhibiting bacterial growth in the tray, this can reduce
contamination on the chamber walls. Add one small teaspoonful (~0.11 oz or 3.6 g) of copper sulphate
to the water in the humidity tray.
3. To reduce the possibility of contamination, every 10 to 14 days, empty the tray, clean it with a solution of
70 % isopropyl alcohol and 30 % distilled water, and then refill it with 0.5 liters of warm distilled water.
The internal chamber will reach ~95 % relative humidity at 37 °C using the 0.5 liter humidity
tray.
5.3 Programming
5.3.1 Programming CO2, O2 and temperature
Perform the following steps to set the desired operating temperature, O2 and CO2 level.
2. In the PROG screen that appears, press the desired function key, TEMP, O2 or CO2, then use the and
direction keys to adjust the value.
If the incubator is supplied with the option of oxygen control, the setpoint for the oxygen level
can be selected and changed like the temperature and CO2 setpoints.
3. When the desired setpoint is displayed, press the ENTER function key.
4. After making adjustments (if any were made), allow the incubator to stabilize at the setpoints before
continuing.
If the chamber temperature goes above the temperature setpoint by 1 °C, the
over-temperature system will activate.
Program the required oxygen level in the PROG screen, following the onscreen instructions.
If you are running an O2 level programmed between 0.1 - 0.9 %, you should know that the
control system is set to operate in the following way to minimize N2 consumption after the
glass door has been opened:
• The N2 valve is switched on continuously until the O2 level is within 0.1 % of setpoint.
• The CO2 valve is then switched on to allow the CO2 level to reach setpoint. If the O2 level is
above setpoint 15 minutes after the N2 valve has been switched off, it is switched back on
for 40 seconds and the CO2 valve is switched on for 20 seconds. The CO2 valve will then
pulse until setpoint is reached.
• The process described above will repeat itself until the O2 setpoint is reached.
• The same process will also repeat if the O2 level rises above setpoint, and if the O2 level
should rise toward 0.2 % above setpoint, the N2 valve will open again continuously until
the O2 level returns to setpoint.
• The CO2 autozero, which would normally take place after a CO2 alarm, will be cancelled to
avoid the introduction of additional O2 into the chamber. For the same reason, we
recommend canceling the programmed CO2 autozero.
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 25
English (EN)
Programmable user access code allows you to restrict access to the PROG, USER, and ALARM screens
(where settings can be changed) to authorized persons only.
If the access code has been misplaced, you will be unable to make changes to your incubator’s
settings. Contact customer service or your service representative for instructions on how to
regain access to your incubator.
Prior to using the incubator, you should manually perform a CO2 autozero:
1. Perform a CO2 autozero by pressing the USER function key (see 48 R control panel on p. 11), selecting
PROGRAMMABLE CO2 AUTOZERO, and pressing the START key.
2. The incubator will display a countdown as the autozero is running.
3. When the countdown is complete, the incubator is ready to use.
Operation - Galaxy 48 R
26 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
In the USER screen, you can adjust the features called out on the screen.
1 Use the and direction keys to move the 2 Use the ENTER function key to select an option
cursor
This section explains each of the USER screen features. There are other USER options that may be
displayed on screen if they are installed on your incubator. For a list of available options (see Available
options on p. 77).
The date and time is factory set and will only require adjustment if you are in a different time zone, or when
you change your clocks to daylight saving time and back again to standard time. You may also select the
style of display for the date.
When you select this feature, the PROGRAM CO2 AUTOZERO screen (see Fig. 5-2 on p. 27) allows you to
program the autozero frequency and time, or to run the autozero function manually.
The autozero system automatically re-references the CO2 sensor to atmospheric CO2 in the following way:
1. A pump activates for two minutes, pumping atmosphere at 0.3 liters/minute into the sensor’s measuring
chamber. This displaces the chamber atmosphere completely from the sensor.
Abb. 5-2: PROGRAM CO2 AUTOZERO screen
This procedure does not affect the internal chamber environment and will not affect your cell
culture as it is being performed.
2. At the end of the two minutes, the control system adjusts the autozero Factor to reference the sensor to
0.05 % CO2, which is the approximate atmospheric level.
3. The pump switches off and the chamber atmosphere diffuses back into the sensor’s measuring
chamber. This takes three minutes, after which the normal CO2 control system takes over.
4. The result of the autozero (listed as A/Z on some screens) is sent to the DATALOGGER ALARM EVENTS
screen so that a record of the results will be kept.
The frequency of autozeroing can be set in steps between once a day and once every 28 days. The default
setting is once every 28 days. If not required, it can be disabled (see DISABLE on p. 28).
The default time setting is 7:00 am. This can be altered to suit your requirements. We recommend that you
only change the time setting shortly before you start to use the incubator.
The autozero will only occur if the temperature is at setpoint. If the temperature is not at
setpoint, the system will postpone autozero until the setpoint is achieved.
If the autozero function is to be run manually, simply press the START function key, within the PROGRAM
CO2 AUTOZERO window.
5.5.4 DATALOGGER
You can adjust the power frequency to either 50 or 60 Hz to match the local mains/electrical supply. Use the
or direction key until the correct frequency is displayed, then press the ENTER function key.
5.5.6 DISABLE
This feature allows you to inform the control system to ignore certain sensors if their function is not
required. The standard item on this menu is the CO2 PRESSURE SWITCH (for autozeroing). Additional
Disable Options appear on this screen according to the options installed on your incubator.
To disable a feature, scroll to OFF using the and direction keys, then press the ENTER function key.
Make sure that the humidity tray is empty and dry, and that the O2 sensor is removed
before running high temperature disinfection.
If the incubator is supplied with the high temperature disinfection option, the menu item DISINFECTION
will be displayed. This feature activates the disinfection cycle of the incubator.
The disinfection cycle heats the inner chamber to 120 °C, holds that temperature for 4 hours, then cools the
chamber to the selected temperature setpoint. All of the interior components (with the exception of the O2
sensors, if present) can be left in place during the cycle to ensure that everything within the chamber is
disinfected prior to resumption of activity. For a full explanation of this feature, (see High temperature
disinfection on p. 36).
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 29
English (EN)
The following alarm events are recorded in the order in which they occurred, with the most recent event
displayed at the top:
• Power ON/OFF
• Chamber temperature high/low (programmed value)
• CO2 level high/low (programmed value)
• CO2 supply failure
• All system alarms
• CO2 autozero (A/Z) adjustments
• Oxygen and relative humidity (R/H) alarms (where these options are installed)
The capacity is 99 events, after which the earliest event is overwritten and a later event is added.
The date and the time are also recorded for each event, (see Fig. 5-4 on p. 30):
Operation - Galaxy 48 R
30 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
When you select this from the DATALOGGER screen, the Door Open bar chart is shown at the top of the
screen to associate it with a temperature disturbance (see Fig. 5-5 on p. 31). A temperature reading is
recorded every 18 seconds while the temperature is outside the specification of ±0.1 °C and each reading is
shown as a single pixel.
When the temperature has settled within specification, the recording is compressed to one pixel
representing (10) 18-second readings (as long as the temperature remains in specification). This allows up
to 10 hours of readings to be displayed on one screen. When the temperature moves outside specification,
for instance if the door is opened, the graph reverts to individual 18-second readings until temperature is
within specification again.
When the data is compressed or decompressed, a light dotted line is displayed vertically on the screen to
signify that the time axis is changing from 18-second to 10 x 18-second increments or vice versa (see
Fig. 5-6 on p. 31).
A heavy dotted line (not shown) is displayed when the incubator is switched on.
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 31
English (EN)
Compressing data allows memory space to be maximized. Once the memory space has been filled, the
earliest events are overwritten as they are replaced by the latest recording. Graphical recording can be
extended, however, to a number of years if your incubator is equipped with an RS-232 port, by connecting
the port to a PC loaded with BioCommand SFI Software (see RS-232 interface on p. 51).
These graphs record in a way similar to the chamber temperature graphs. The specification for CO2 is ±
0.1 %.
Both CO2 and temperature graphs share the same time axis. If the time axis changes to
accommodate data in one graph, it will also change in the other graph.
Operation - Galaxy 48 R
32 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
This graph records chamber element temperature over time to assist troubleshooting.
This graph records the door’s inner surface temperature over time to assist troubleshooting.
This graph records door element temperature over time to assist troubleshooting.
This feature removes the current graph and begins a new one. The data cannot be recovered once it is
deleted.
To enter the CHAMBER ALARMS menu screen, press the ALARM function key on the main display. The
CHAMBER ALARMS programming screen (see Fig. 5-7 on p. 32) allows the various alarm options to be
selected and modified. Press the or direction key to move around the options and the or
direction key to adjust values. The temperature and CO2 High and Low Alarm setpoints automatically adjust
to within ± 0.5 of the temperature and CO2 setpoints. The alarm setpoints can also be manually adjusted.
Alternatively, the alarm system can be set to re-arm only after the original temperature and CO2 setpoints
have been achieved:
1. Choose the option ARM ALARMS WHEN AT SETPOINT.
2. Select YES for both TEMP and CO2.
When YES is selected for this function, the DELAY IN ARMING AFTER DOOR OPEN is ignored.
A DOOR OPEN ALARM: can be adjusted, choosing from seven preset durations (45 seconds in (Fig. 5-7 on
p. 32)) to warn of an improperly closed door.
The AUDIBLE and VISUAL alarms can be adjusted from OFF to ON (which means the alarm will be on
continuously until it is acknowledged) in seven preset time increments.
In the OFF position, any Chamber Alarms that occur will be displayed on the screen without flashing and
with the audible alarm inhibited (see Fig. 5-8 on p. 33).
When the incubator is switched ON, or after the temperature and CO2 levels have been re-programmed, the
alarm system is inactive until the setpoint values are achieved (within ± 0.1), after which the alarm system
is armed. CO2 and temperature alarms are individually armed.
If temperature and/or CO2 levels deviate more than the programmed setpoints, the display flashes, the
audible alarm sounds and a message appears on the screen (see Fig. 5-8 on p. 33). Acknowledge the alarm
by pressing any key.
After setpoints have been achieved for the first time, when the outer door is opened, the alarm system is
disabled; on closing the door, if selected, a programmable alarm delay starts:
Operation - Galaxy 48 R
34 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
• If chamber conditions recover within the programmed alarm delay time, the alarm system is
immediately re-armed. After the delay, the alarm system is armed and if the temperature and CO2 are
outside the alarm high and low settings, the alarm will be activated.
• If an alarm occurs and the chamber subsequently recovers, the alarm stops and the system is re-armed.
Details of the alarm event are stored in the datalogger.
If the CO2 valve is opened and no pressure is detected, an alarm occurs and a warning message appears on
the screen, alerting you to CHECK CO2 SUPPLY (see Fig. 5-9 on p. 34).
The alarm levels are set automatically to ± 0.5 % above and below the programmed value, but
these points can be altered in the ALARM screen. In addition, the re-arming of the alarm can
be delayed until the Programmed Value is achieved by selecting the relevant option in the
ALARM screen.
For example, it is possible at low oxygen levels that the CO2 and O2 levels might not fully
recover within the Alarm Limits after the 15-minute DELAY IN ARMING AFTER DOOR
OPENING period has elapsed, so this time period can be increased in the ALARM screen to
suit individual circumstances.
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 35
English (EN)
The DIAGNOSTICS screen contains technical information regarding the status of many of the system
components found on the incubator. This screen is mainly for technical service use, and can be used to
troubleshoot the incubator systems before service is scheduled. This information allows technical support
to optimize the service support required, and to shorten service time.
The HELP MENU screen provides user-selectable categories of abbreviated information found in the user
manual. All the major systems are covered in the help menu, including help on installing the incubator. If
the user manual is misplaced, information about the CO2 incubator and its functions can always be found
on-screen.
The high temperature disinfection option is designed to heat the internal chamber to 120 °C, maintain that
temperature for 4 hours, and then allow the chamber to cool down to 37 °C or to the programmed
temperature (if different from 37 °C) when normal control takes over. The cycle is designed to disinfect all
internal surfaces and components, with the exception of the oxygen control sensor where supplied (see
High temperature disinfection option with oxygen control on p. 38).
Prerequisites
• The incubator should be cleaned, disinfected, and dried thoroughly before starting the cycle, (see
Cleaning on p. 68).
• The black protective cover must be removed (the white porous cover can remain in place).
• The shelves, shelf racks, humidity tray and silicone rubber feet and sleeves should all be in place during
the cycle.
• The incubator MUST be clean and dry.
• The humidity tray MUST be empty, clean and dry.
Do not touch the equipment during the high temperature disinfection cycle.
Do not open equipment door during the cycle.
To avoid possible damage to the CO2 sensor, never leave water in the humidity tray while
the incubator is switched off, or when a high temperature disinfection cycle is initiated
(optional feature).
Allow a clearance of 15 – 20 cm (6 – 8 in) to allow access for oxygen sensor removal.
1. Press the USER menu button, select DISINFECTION and press START. The incubator will then prompt:
IS CHAMBER CLEAN & DRY? Answer YES if it is clean and dry.
The cycle will start automatically, unless the incubator is fitted with oxygen control, in which case the
incubator will also prompt: IS O2 SENSOR REMOVED? Ensure that the O2 sensor has been removed and
answer YES to begin the cycle.
2. To cancel the cycle, press CANCEL. The incubator will cool down to the programmed level where
normal control takes over.
If an autozero is scheduled to begin prior to a disinfection cycle, the autozero will abort until
the cycle is complete. A user initiated autozero will also abort but will not resume after
completion of the disinfection cycle.
3. If the incubator door is opened during a disinfection cycle, the process will continue as normal, a failure
message will occur due to low temperature.
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 37
English (EN)
Certain areas of the glass door and inner door seal surface temperatures will be ± 5° of 120 °C.
4. After completion of the process, one of the following status messages will be displayed. If the cycle:
was completed successfully, DISINFECTION COMPLETED OK is shown.
was cancelled by the user, DISINFECTION WAS ABORTED is shown.
failed for any reason, DISINFECTION FAILED [CODE: XX] is shown.
The following tables lists the disinfection failure codes and descriptions, (see Tab. on p. 37) and (see
Tab. on p. 37). If this happens, note the failure code and contact your service representative for advice.
• If the incubator power is cycled OFF then ON during a disinfection cycle due to a power
outage, the incubator will power up as normal. This condition will be indicated by the
absence of a completed disinfection status message (DISINFECTION COMPLETED OK).
• If the chamber temperature is above the setpoint or the element temperature is greater
than a factory-preset control point, cool down will be entered until these conditions are
satisfied.
• It is recommended that the autozero function be run following each disinfection cycle.
To avoid possible damage to the CO2 sensor, never leave water in the humidity tray while
the incubator is switched off, or when a high temperature disinfection cycle is initiated
(optional feature).
Allow a clearance of 15 – 20 cm (6 – 8 in) to allow access for oxygen sensor removal.
The oxygen sensor is an electrochemical device that will be destroyed by the high temperature used to
disinfect the incubator if left in place. For this reason, the oxygen sensor must be removed from the
incubator prior to a high temperature disinfection cycle. The sensor can be accessed from the rear panel of
the incubator.
Detailed removal and installation instructions are provided (see Oxygen sensor removal on p. 38).
Prior to beginning a disinfection cycle, remove the oxygen sensor. For detailed instructions, (see Removing
and replacing O2 sensor on p. 47).
1. Remove the black plastic plug that covers the oxygen sensor located on the back of the incubator.
2. Disconnect the sensor cable by unplugging the electrical connector (grasp the white connector body,
not the wire leads).
3. Unscrew the oxygen sensor by turning it counter-clockwise. Never use excessive force or metal tools.
4. Store the sensor in a clean, safe place until the disinfection cycle is over.
5. The incubator is now ready to perform a disinfection cycle.
The BMS (Building Management System) relay contact alarm allows a signal from a central alarm system to
be switched ON or OFF to indicate an alarm condition at the incubator.
• Over-temperature
• Under-temperature
• System failure
• CO2 high
• CO2 low
As an integral option, the alarm can be programmed to indicate when the power fails (perhaps due to an
electrical fault) or is switched off. If the power failure warning is active, the relay contacts will be reversed
(pin 4, which is normally open, becomes normally closed and pin 6, which is normally closed, becomes
normally open). The alarm will also respond to other types of alarms, depending on the options installed on
the incubator.
The system is connected at the rear of the incubator via a standard 6-pin DIN socket (see Fig. 5-12 on p. 39)
for location. The matching plug is provided, when the option is installed.
2
4
1 5
Pin Designation
1 48R: 24 V DC To power external equipment such as a remote buzzer or light
unregulated (100 mA maximum current available).*
48S: 12 V DC
2 0V
3 5 V DC Via 10 K Ω pull-up resistor, for a logic signal to directly control an
auxiliary control system.*
4 Normally closed To access the relay contacts. Contact limits are 3 Amps @ 24 V DC
5 Common and 3 Amps @ 34 V AC.
6 Normally open
*Cable length should not exceed 3 m (9.8 ft) to comply with EMC requirements.
Operation - Galaxy 48 R
40 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
The default of Galaxy 48R setting for the alarm system is ON. To deactivate the relay using the incubator
keypad:
1. Press USER.
2. Select BMS ALARM RELAY.
3. Select MAKE ALARM RELAY ACTIVE YES/NO.
4. Toggle to NO and then press ENTER.
To enable or disable BMS on the Galaxy 48S model, simply go to the Engineering menu, scroll down to
RELAY, then press ENTER. Using the up / down arrow keys, choose either YES or NO.
The default setting for the power failure warning is ON. To make the alarm system ignore any power
outage:
1. Press USER.
2. Select BMS ALARM RELAY.
3. Select MAKE ALARM RELAY ACTIVE AT POWER SWITCH OFF/FAILURE YES/NO.
4. Toggle to NO and then press ENTER.
5.13 O2 control
5.13.1 Setting up the N2 tank
Before setting up O2 control, ensure that you have the proper equipment for your nitrogen supply:
1. Inspect the tanks to ensure that there are no leaks or other damage.
2. Check that the two-stage pressure regulator valve and the inline pressure regulator valve are closed by
trying to turn their knobs in a counter-clockwise direction.
The knobs should be at the stop point.
3. Place the inline pressure regulator close to the incubator.
4. Securely attach the two-stage pressure regulator to the nitrogen tank’s outlet and to the inline pressure
regulator’s inlet with tubing, securing both ends with clips.
5. Connect the inline pressure regulator’s outlet to the incubator’s N2 inlet on the right side of the control
box at the rear of the incubator; secure both ends with clips.
6. Set up oxygen control based on your option: 1 – 19 %, 0.1 – 19 %, or 1 – 95 %.
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 41
English (EN)
Oxygen control is tailored such that both the O2 and the CO2 levels are achieved at approximately the same
time, via control of the N2 valve's duty cycle (but within the range of 0.1 – 19 % O2)
1. Remove the black plastic protective cover (making sure that the hydrophobic filter cap is not removed
with it) from the port inside the chamber. Retain the cover for use when you clean the chamber.
2. Open the nitrogen gas supply from the tank and set the N2 tank’s outlet pressure gauge to 1.5 bar.
3. Set the nitrogen regulator to 7 PSI (0.5 bar). The gas flow rate is approximately 20 liters/minute.
When working at high O2 levels approx > 90 %, you may adjust the O2 pressure up to 10 PSI
(0.7 bar).
• If the programmed O2 level is close to the ambient oxygen, it may be necessary to reduce
the cylinder pressure below 1 bar to stop the oxygen level from undershooting the
programmed value.
• When working at 0.1 % O2, set the CO2 pressure to 10 PSI (0.7 bar).
Be sure to humidify the incubator and leave it overnight to stabilize before proceeding further.
To automatically calibrate the sensor to atmospheric oxygen levels, select OXYGEN SENSOR – REF TO
ATMOSPHERE in the USER menu, and then follow the onscreen instructions.
The oxygen reading is automatically adjusted to 19.7 %, which is the true reading taking into account the
relative humidity in the chamber.
Under normal humidity conditions (95 - 99 % RH), the sensor is unaffected. If for any reason
(such as a large spill inside the chamber or the incubator being switched off while fully
humidified) liquid condenses around the sensor, the result may be restriction of gas flow and a
low sensor signal. Should such condensation appear on the chamber walls, normal operation
can be easily restored by removing the humidity tray, drying the chamber completely, and
then running the incubator at 37 °C for one hour. This will dry out the sensor. After the hour
has elapsed, the humidity tray can be reinstalled and the incubator humidified again.
This oxygen control option is designed to cover the 1 – 19 % range by adding nitrogen to bring the level
below ambient. If you have the 0.1 – 19 % oxygen control option, (see Setting up oxygen control (0.1 –
19 %) on p. 41). If you have the 1 – 95 % oxygen control option, (see Setting up oxygen control (1 – 95 %)
on p. 43).
1. Remove the black plastic protective cover from the hydrophobic filter cap (making sure that the
hydrophobic filter cap is not removed with it), located in the rear wall of the incubator chamber. Retain
the cover for use when you clean the chamber.
2. Open the nitrogen gas supply from the tank. Set the N2 tank’s outlet pressure gauge to 1.5 bar
3. Set the nitrogen regulator to 14.5 PSI (1 bar). The gas flow rate is approximately 20 liters/minute.
Operation - Galaxy 48 R
42 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
If the programmed O2 level is close to the ambient oxygen, it may be necessary to reduce the
cylinder pressure below 1 bar to stop the oxygen level from undershooting the programmed
value.
4. After the incubator has been humidified and left overnight to stabilize, select the USER menu; then,
using the or direction key, select OXYGEN SENSOR-REF TO ATMOSPHERE and follow the
onscreen instructions to automatically calibrate the oxygen sensor to atmospheric oxygen levels. The
oxygen reading is automatically adjusted to 19.7 %, which is the true reading taking into account the
relative humidity level.
Abb. 5-13: Selecting OXYGEN SENSOR-REF TO ATMOSPHERE
7. The alarm levels are automatically set to ± 0.5 % above or below the programmed value, but you can
modify them in the ALARM screen. Re-arming the alarm can be delayed until the programmed value is
achieved: select the relevant option in the ALARM screen.
At low oxygen levels, the CO2 and O2 levels may not have fully recovered within the Alarm
limits after the 15-minute “Delay in arming after door opening.” This time period can be
increased (in the ALARM screen) to suit individual circumstances.
8. By controlling the duty cycle of the N2 valve, oxygen control can be tailored to achieve programmed
oxygen and carbon dioxide levels at approximately the same time.
We recommend that you repeat the OXYGEN SENSOR-REF TO ATMOSPHERE procedure (see Referencing to
atmosphere on p. 45) Step 3, and further details once a month to ensure that any long-term drift in sensor
output will be corrected. Be sure to do it at the chamber operating temperature.
When you are cleaning the chamber, be very careful not to wet the oxygen sensor or CO2 sensor. Never use
solvents on the sensor membrane; rather, be sure to cap the hydrophobic filter before you clean. It is good
practice to replace the filter each time you clean the incubator chamber, to avoid the possibility of filter
contamination.
Under normal relative humidity conditions (95 – 99 %), the oxygen sensor’s performance should not be
affected. If, however, liquid condenses around the sensor, gas flow may become restricted, giving the
sensor a low signal. This may occur if there is a large liquid spill inside the chamber or if the incubator is
turned off while it is fully humidified. Should such condensation appear, normal operation can be restored
by:
This will dry out the sensor(s). The humidity tray can then be reinstalled and the incubator can be safely
re-humidified.
This option uses the controlled addition of oxygen to increase the level above ambient and the controlled
addition of nitrogen to reduce the oxygen level below ambient. If you have the 1 – 19 % control option, . If
you have the 0.1 – 19 % control option, .
Operation - Galaxy 48 R
44 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
Setting the oxygen to levels > 24 % can greatly increase the chance of fire and explosion.
Operators planning to set oxygen at 24 – 95 % should therefore take all appropriate
precautions to minimize the risk. Eppendorf does not recommend using external
(heat-generating) equipment inside the chamber when oxygen conditions will be > 24 %.
Before opening the incubator door, make sure no flames (Bunsen burner, etc.) are in the
vicinity.
Avoid the presence of other combustible gases (hydrogen, methane, etc.)
Ensure that all solenoid valves and pressure regulators and gauges controlling the gas
supply are grease-free for use with oxygen.
If your incubator is operating in the 3 – 95 % range of O2 control, only the N2 valve will operate for
setpoints below 19 %; both the N2 and O2 valves will operate for setpoints between 19 and 80 %; and for
setpoints above 80 %, only the O2 valve will operate.
If you plan to maintain O2 levels of 80 – 95 %, please read the important notes at the end of
the following procedure.
Oxygen control is tailored such that both the O2 and the CO2 levels are achieved at approximately the same
time, via control of the O2 or N2 valve’s duty cycle.
1. Remove the black plastic protective cover (making sure that the hydrophobic filter cap is not removed
with it) from the port inside the chamber. Retain the cover for use when you clean the chamber.
2. Open the nitrogen gas supply from the tank and set the N2 tank’s outlet pressure gauge to 22 PSI (1.5
bar).
3. Set the nitrogen regulator to 7 PSI (0.5 bar). The nitrogen will be fed into the incubator through a filter
that is already installed in the gas line. The gas flow rate is approximately 20 liters/minute.
If the programmed O2 level is close to the ambient oxygen, it may be necessary to reduce the
cylinder pressure below 1 bar to stop the oxygen level from undershooting the programmed
value.
4. Install the oxygen cylinder in the same manner as outlined for the nitrogen cylinder installation (see
Setting up the N2 tank on p. 40), using the incubator’s O2 inlet, of course. For this application, you will
need another two-stage pressure regulator and another inline pressure regulator.
Be sure to humidify the incubator and leave it overnight to stabilize before proceeding further.
To automatically calibrate the sensor to atmospheric oxygen levels, select OXYGEN SENSOR – REF TO
ATMOSPHERE in the USER menu, and then follow the onscreen instructions.
The oxygen reading is automatically adjusted to 19.7 %, which is the true reading taking into account the
Relative Humidity in the chamber.
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 45
English (EN)
CO2 regulator should be set to 7 PSI (0.5 bar) if O2 is set at or above ambient (i.e.
approximately 19%) in a humidified atmosphere, 5 PSI (0.35 bar) if at levels below ambient.
If you are running the incubator with a dry chamber and no CO2, the recommended maximum
O2 level is 95 %. In addition, open samples inside the chamber can cause RH to increase, and
the O2 sensor may require recalibration to work properly in a completely dry atmosphere.
For a humidified chamber with no CO2, the recommended maximum O2 level is 92 %, while
for a humidified chamber with 5 % CO2, we recommend no more than 87 % O2.
When the incubator is running at normal humidity level (i.e., 95 % RH), there is
approximately 6 % (by volume) water vapor present in the chamber. If 5 % CO2 is also
present, the maximum achievable level of O2—without using excessive quantities of oxygen—
is approximately 87 %. Oxygen direct from a cylinder has a dewpoint temperature of <-45°C,
corresponding to 0.06 % water vapor. As it enters the incubator, therefore, its temperature is
very low and even as it is warmed, its RH level is very low. Both of these factors affect the
performance of the O2 detector. To minimize these effects, O2 is fed continuously until it
arrives at 0.4 % below setpoint. To allow the detector signal to recover and the RH level to
build, there is a 3-minute delay. If O2 is still required, it is fed in for 10 seconds, with an
additional 3-minute delay until setpoint is achieved.
As the humidity level rises, the O2 content in the chamber will diminish. The use of O2 will rise
considerably as the setpoint approaches the sum contents of water wapor and CO2 in the
chamber. In the short term, it is possible to achieve higher levels of O2, but as humidity rises
and O2 content is pushed down, there will be a continuous demand for O2 and RH will remain
too low. At the same time, as O2 is pumped in, the CO2 level will also diminish, causing the
system to add CO2, which in turn causes the O2 level to drop again.
If the O2 setpoint is too high, the incubator can consume some 2,500 liters of O2 per day—
which does not include the extra oxygen consumed each time the door is opened (about 500
liters).
The oxygen sensor is a self-powered electrochemical cell with a finite life that is dependent on the ambient
oxygen level. A typical lifespan is 1 – 2 years at atmospheric levels. During the sensor's lifespan, the signal
produced will slowly degrade until it is ultimately unuseable. For this reason, we recommend that you
reference the sensor to atmospheric oxygen levels on a monthly basis.
To reference the sensor to atmosphere, enter the USER menu and select OXYGEN SENSOR-REF TO
ATMOSPHERE.
1. The test was completely successful, and no further action is needed until the next month's test.
2. The sensor needs to be replaced soon (see Replace sensor soon on p. 46).
The sensor needs to be replaced immediately (see Replace sensor now on p. 46).
Operation - Galaxy 48 R
46 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
If you complete a referencing procedure and the O2 sensor is not functioning at 100%, you will recieve 1 of
2 messages: O2 REFERENCE OK BUT SENSOR REQUIRES REPLACEMENT SHORTLY (see Replace sensor
soon on p. 46), or O2 REFERENCE FAILED (see Replace sensor now on p. 46) if the sensor is no longer
functioning.
If the referencing procedure was successful but the sensor is nearing the end of its working life, the
following message will appear in the display:
When you press the ENTER function key, the message will change to this:
O2 SENSOR
THE RESULT OF THE O2 REFERENCE PROCESS SHOWS THAT THE SIGNAL FROM THE O2 SENSOR HAS
REDUCED INDICATING IT IS APPROACHING THE END OF ITS LIFE.
If the referencing procedure failed, oxygen control will be disabled. The incubator will appear to be
functioning as normal until a new sensor is installed and referenced to atmospheric level. The following
message will appear in the display:
O2 REFERENCE FAILED
When you press the ENTER function key, the message will change to this:
O2 SENSOR
THE RESULT OF THE O2 REFERENCE PROCESS SHOWS THAT THE SIGNAL FROM THE O2 SENSOR HAS
REDUCED BELOW AN ACCEPTABLE LEVEL AND HAS REACHED THE END OF ITS LIFE.
When you press the NEXT function key, the message will change to this:
O2 SENSOR
OXYGEN CONTROL HAS BEEN DISABLED AS A RESULT BUT THE INCUBATOR IS OTHERWISE FULLY
OPERATIONAL.
When you press the EXIT function key, you will return to the USER screen and normal operation.
Prerequisites
• Oxygen sensor removal tool (see Fig. 5-16 on p. 48)
To remove and replace the oxygen sensor, you will need the sensor removal tool provided (see Fig. 5-16 on
p. 48).
1. Pull the rear access cover off the rear outside wall of the incubator to gain access to the oxygen sensor.
Abb. 5-15: Oxygen sensor rear access cover
2. Reach inside and disconnect the sensor by unplugging the connector: be sure to grasp the white
connector body.
3. Using the sensor removal tool (see Fig. 5-16 on p. 48), unscrew the oxygen sensor by turning it
counter-clockwise. Because the oxygen sensor contains lead, be sure to dispose of it according to local
regulations.
4. Using the sensor removal tool, install the new oxygen sensor by turning it clockwise until it is
finger-tight. Do not use excessive force or any metal tool.
Abb. 5-16: Oxygen sensor removal tool
5. Reconnect the sensor by plugging the white connector body back in.
6. Make sure that the sensor wires are inside the metal sensor tube to protect them from damage, then
press the rear access cover snugly back in place.
7. After replacing the sensor, humidify the incubator and allow it to stabilize overnight.
8. Calibrate the sensor with reference to the atmospheric oxygen level (see Referencing to atmosphere on
p. 45).
The hydrophobic filter installed on your oxygen control system helps prevent condensation from reaching
the sensor.
1. Carefully pull the complete hydrophobic filter holder away from the oxygen sensor holder on the rear
wall of the chamber.
2. With a fingertip or a 10 - 11 mm (½ in) rod, from the rear of the filter holder, gently push the filter
membrane disc and the filter cap out of the holder.
3. Clean and dry the filter holder and cap.
4. Wearing gloves to avoid contaminating the filter disc, gently place the new filter membrane disc into the
filter holder recess. The filter disc works in both directions, so there is no right or wrong side.
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 49
English (EN)
1 2 3
5 4
5. Make sure the O-rings (between the cap & filter holder and between the filter holder & oxygen sensor
holder) are undamaged and securely in place.
6. Gently press the filter cap back in.
7. Press fit the filter assembly back onto the oxygen sensor holder.
The humidity alert package includes a humidity tray warning system and humidity display and alarm
function, both providing optimal feedback on relative humidity inside the incubator chamber.
The humidity tray warning system is designed to prevent the water level in the humidity reservoir from
becoming too low. The water level is continuously measured by an optical water level sensor. If the water
level in the humidity tray become too low a HUMIDITY WATER LOW ALARM is displayed. This alarm can be
cancelled by pressing any key, but it will re-activate when the door is opened and closed.
Operation - Galaxy 48 R
50 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
Never leave the humidity warning sensor on the bottom surface of the incubator during a
high temperature decontamination cycle. Place it on the shelf above the humidity tray and
ensure that the cable is not resting on the base of the incubator chamber.
1. Press USER.
2. Select DISABLE and toggle HUMIDITY WARNING from ON to OFF using the keys.
3. Press ENTER.
4. Refill the humidity tray with 0.5 liters of warm (~37.0 °C) distilled water.
When the water level has been replenished and the door is closed the system will re-arm.
The humidity display and alarm system is designed to measure the relative humidity level (rH) in the
chamber and to display the chamber rH on the display screen. Under normal operating conditions and
using the supplied humidity tray, the rH level will reach a maximum of 95 - 96 % after being left overnight.
The humidity sensor will activate an alarm if relative humidity falls below the preset limit of 88 %. The
sensor is located on the rear wall of the chamber, below the CO2 Sensor. The humidity display is always
active, but the alarm system can be deactivated as follows:
1. Press USER.
2. Select DISABLE, and toggle RELATIVE HUMIDITY from ON to OFF using the keys.
3. Press ENTER.
The alarm system is disarmed for 1 hour or when it achieves 88% R/H, when the incubator is switched on,
or if the door is opened and closed.
An alarm is signaled by a flashing RH LOW ALARM message. If the humidity level rises above 88 % during
the 1 hour time out period, the humidity alarm system will be armed. The alarm will then be triggered when
the R/H level falls below 88 % and will be recorded by the alarm log. The alarm can be acknowledged by
pressing any key. No further alarms will occur unless the door is opened and closed, or power to the
incubator is removed then restored.
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 51
English (EN)
The humidity sensor is protected by a white porous plastic cover, care must be taken not to
spill any liquid into the sensor. The white porous cover should be protected with the black
plastic sleeve provided when cleaning the incubator. The white porous cover can be removed
and autoclaved but care should be taken not to touch or wet the sensor when the cover is
removed.
An external computer can be connected to the serial interface for data logging using optional BioCommand
SFI software, or by remote control with a communication program. Data from the incubator can be
downloaded for record keeping or validation documentation. The device can be controlled remotely and
operating parameters can be transmitted and recorded using third party software. Information on installing
and operating the communication program can be found in the corresponding software documentation.
The RS-232 interface port provides a connection for the incubator to a personal computer or terminal. This
allows the incubator’s operating status to be viewed on the screen.
The computer should be connected to a mains/power supply outlet as close as possible to the
incubator.
Prerequisites
• A PC or terminal, capable of RS-232 communication, with one serial com port free for this connection or
a serial interface box (see Main illustration on p. 9).
• A null-modem screened cable of suitable length. To ensure reliable communication, the cable should
not exceed 15 m (49 ft) in length. One end must have a 9-pin female D connector, to connect to the
incubator’s RS-232 port, and the other end must have either a 9-pin or 25-pin female D connector,
whichever mates to your PC or terminal (see Main illustration on p. 9).
• Suitable communications software, such as New Brunswick BioCommand SFI or HyperTerminal
(included with Windows® NT, 95, 98, ME, 2000 and XP).
1. Connect the null-modem cable to the RS-232 port at the rear of the incubator.
2. Connect the other end of the cable to either the 25-pin male D serial com port or the 9-pin male D serial
com port on the PC or terminal.
3. Turn on the computer and start the communications software.
Operation - Galaxy 48 R
52 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
4. Select an unused serial port to enable communications between the incubator and the PC or terminal. If
you know which port to use, continue to Step 5 and then skip to Step 7. If not, continue to Steps 5 and 6.
5. Using the communications software, select the unused port (COM1, COM2, COM3, etc.) then press the
ENTER key on the PC/terminal keyboard (refer to BioCommand SFI operating manual for instructions).
If you have successfully chosen an unused port, the message Type ? for help should appear on the
computer screen.
6. If you see any other message, or nothing happens, select the next port and press the ENTER key again.
If you still do not see the expected message, change the Flow Control setting from Hardware to None,
then try again.
7. When the connection between the incubator and PC/terminal has been successfully established, and the
message indicated in Step 5 appears, type (as prompted by the message onscreen) a ? in the
communications software, then press the ENTER key.
8. This menu will appear on the computer screen:
This menu allows the incubator temperature, CO2 and alarm setpoints to be programmed remotely. A
running status report can be generated at user-defined intervals, and all reports can be sent to a printer. For
details on these features, consult the documentation supplied with your communications software.
Remote programming: all commands beginning with P can be used to program the incubator from the
remote computer. These commands are not case sensitive: lower case or upper case letters work exactly
the same way. The following are examples of how each of the above commands might be used:
Operation - Galaxy 48 R
Galaxy® 48 R/48 S CO2 Incubators 53
English (EN)
In each case, the change is confirmed by a message from the incubator (e.g., Program Temperature 37.5 °C
OK, or Program Alarm Temperature High 38.0 °C OK, etc.).
Status commands: all commands beginning with S can be used to query the incubator from the remote
computer, and to display the current status of the incubator. As with the program commands, these
commands are not case sensitive: lower case or upper case letters work exactly the same way. The
following are examples of how each of the status commands might be used:
Other commands: As with the Program and Status commands, the V (Version number report) and ? (Help)
commands are not case sensitive. To use these two commands:
Alarm/Event Messages: a number of messages are preset to appear on the computer screen to inform you
of either an alarm condition or a certain event. The following table recaps those messages:
After displaying any of the above messages, a status report message relative to the alarm or event will be
displayed, e.g.:
6 Operation - Galaxy 48 S
6.1 Preparing for operation
1. Remove the black protective cover from the CO2 sensor, taking care not to remove the white porous
cover.
Store the black cover on the sensor cover holder on the back of the incubator . The sensor cap should be
placed back on the sensor when the incubator is to be cleaned.
2. Ensure that the white porous sensor cover remains in place.
3. Using the mains/power cord provided, connect the incubator to a earthed/grounded mains/power
supply.
4. Switch the incubator ON using the on/off switch at the rear of the cabinet.
The display will illuminate immediately.
5. Turn on the CO2 gas supply with the pressure regulator set to 5 PSI (0.35 bar) unless operating with N2
or O2.
6. The chamber setpoints are pre-programmed at 37.0 °C and 5 % CO2. Leave the incubator on until the
programmed chamber temperature and CO2 concentration have been reached.
• The incubator’s CO2 valve is disabled until the incubator reaches the temperature setpoint.
After the temperature setpoint is reached, the CO2 valve is activated, allowing the
incubator to reach the CO2 setpoint.
• If power is interrupted to the incubator long enough for the temperature to drop below
setpoint, the CO2 valve will be deactivated until temperature setpoint is again achieved.
(This serves to avoid spurious CO2 readings while the incubator is below its temperature
setpoint)
7. Leave the incubator running for at least two hours (preferably overnight) to allow conditions to stabilize.
To avoid possible damage to the CO2 sensor, never leave water in the humidity tray while
the incubator is switched off, or when a high temperature disinfection cycle is initiated
(optional feature).
Allow a clearance of 15 – 20 cm (6 – 8 in) to allow access for oxygen sensor removal.
If humidification is required:
1. Fill the humidity tray with 0.5 L of warm (around 37.0 °C) distilled water.
2. For cell culture work, we recommend adding a very small amount of copper sulphate in the humidity
tray. Tests have shown that, in addition to inhibiting bacterial growth in the tray, this can reduce
contamination on the chamber walls. Add one small teaspoonful (around 0.11 oz or 3.6 g) of copper
sulphate to the water in the humidity tray.
3. To reduce the possibility of contamination, every 10 to 14 days, empty the tray, clean it with a solution of
70 % isopropyl alcohol and 30 % distilled water, and then refill it with 1.5 liters of warm distilled water.
The internal chamber will reach approximately 95 % relative humidity at 37 °C using the 1.5 L
humidity tray.
Perform the following steps to program the temperature and CO2 setpoints. Temperature and CO2 may be
set within the ranges shown in the following table:
2. Press the Up ( ) or Down ( ) key until the desired value appears in the left-hand display.
3. Press the Enter key to save the setpoint.
The temperature display will stop flashing, and the CO2 display will flash.
Set the CO2 level.
1. Press the up ( ) or down ( ) key until the desired value appears in the right-hand display.
2. Press the Enter key to save the setpoint.
CO2 gas will NOT be injected until the incubator reaches its temperature setpoint.
Allow the incubator to stabilize at the setpoints selected before continuing.
Operation - Galaxy 48 S
Galaxy® 48 R/48 S CO2 Incubators 57
English (EN)
The purpose of the autozero system is to reference the CO2 sensor to an atmospheric CO2 level of 0.05 %.
Over a period of time, the sensor baseline may drift, causing an inaccuracy in the CO2 level at the
programmed value.
1. If you have not already done so, set the CO2 level (see Setting temperature and CO2 on p. 56). There
should be an audible click when the programmed level is entered: this is the CO2 valve opening.
Autozero can be performed with the incubator programmed at a particular set point and the
chamber is immersed in CO2, or whether the incubator is programmed at 0.0 % and the
chamber is immersed in ambient air.
2. Press the and Enter keys simultaneously.
There is a magnetic switch above the door, and a magnet mounted in the door, to switch off
the CO2 valve when the door is opened.
3. The door (and inner doors, if present) must be opened and closed according to the displayed
instructions, after which the process is completely automatic.
4. Open the door for 60 seconds to completely degas the chamber. A countdown is shown on the display.
5. When the display instructs you to do so, close the door. An automatic 10-minute countdown will start, to
allow the chamber conditions to recover and the CO2 sensor signal to stabilize.
6. At the end of the countdown, the sensor signal will automatically be re-referenced. If successful the
display will flash. Press Programming key to accept.
The incubator will reopen the CO2 valve to allow the CO2 level to recover to the setpoint value.
Pressing any key, or opening the door during the autozero will automatically terminate the
process and control of CO2 will restart.
1. Press the Enter and keys simultaneously to enter the alarm menu.
The display will show: °C.AL.
2. Press the Enter key to display the High Temperature Alarm, HI 37.5. The factory setting is the setpoint
value (37.0 °C) + 0.5 °C.
Operation - Galaxy 48 S
58 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
3. Use the Up ( ) or Down ( ) key to adjust the High Temperature Alarm. The minimum setting is 0.5 °C
from setpoint.
4. Press the Enter key to save the setting.
The Low Temperature Alarm is displayed, LO 36.5. The factory setting is the setpoint value (37.0 °C) -
0.5 °C.
1. Press the Enter and keys simultaneously to enter the alarm menu.
The display will show: °C.AL.
When the door is opened, an alarm will sound after a preset time delay. To adjust the time delay:
2. Press the Enter key and the and keys to adjust the time (as you scroll through the available
choices, you will see 15, 30, 45, 60, 75, 90 seconds, then OFF).
3. Press the Enter key to save the desired value.
1. From the alarm menu, press the key until the display shows PEr .Iod (reading across both displays).
2. Press the Enter key and the and keys to adjust the Alarm duration.
Operation - Galaxy 48 S
Galaxy® 48 R/48 S CO2 Incubators 59
English (EN)
As you scroll through the available choices, you will see OFF, 10 SEc, 30 SEc, 60 SEc, 600 SEc, 1 HR,
then On).
3. Press the Enter key to select the desired value.
The alarm arming delay is the length of time that is allowed for the Temperature and CO2 to recover after
opening the incubator before the Alarm System is armed again. This helps to prevent unnecessary alarms
from occurring.
1. From the alarm menu, press the key until the display shows dr .dEL (across both windows).
2. Press the Enter key and the and keys to adjust the Alarm Arming time.
As you scroll through the available choices, you will see 0.15 Hr, 0.20, 0.30, 1.00 , then OFF.
3. Press Enter to select the desired value.
4. Press to return to the main alarm programming menu, and press again to return to the main
display.
When alarm delay is set to OFF, the Alarms will arm only when the programmed setpoint is
reached.
When the incubator is switched ON, or after any values have been reprogrammed, the Alarm System is
inactive until the setpoint values (± 0.1) are achieved, after which the Alarm System is armed.
If temperature and/or CO2 levels deviate more than the programmed amount, the display flashes, the
audible alarm sounds and a message appears on the screen. You can acknowledge (and cancel) the alarm
by pressing any key.
When the outer door is opened, the Alarm System is disabled. When you close the door, the preset Alarm
Arming Delay starts. When the delay time expires, the Alarm System is re-armed; if the temperature and/or
CO2 fall below or above the alarm setpoints, the alarm will be activated. If chamber conditions recover
within the Alarm Arming Delay time, the Alarm System will be re-armed but no alarm will be activated.
If an alarm is not acknowledged but the chamber conditions subsequently recover, the audible alarm will be
cancelled but the alarm message will remain on the screen to alert the user to the fact that an alarm has
occurred. You can cancel this alarm message by pressing any key. The duration of the audible alarm can be
adjusted from inactive to continuous (see Programming the alarm system on p. 57).
There are four temperature sensors: two in the chamber, and two in the door. If any of these sensors should
fail, the following message will appear: ºC FAIL.
Operation - Galaxy 48 S
60 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
Because the incubator can no longer control temperature properly without the failed sensor, the heating
will switch off and the incubator will cool down to room temperature.
If, however, a sensor fails but subsequently corrects itself, the temperature control will restart and an alarm
message will remain on the temperature side of the display: SAL … (meaning Sensor Alarm).
This alarm only occurs if the chamber temperature exceeds the temperature setpoint by 1 °C or if one of the
heating elements exceeds a specific activation threshold. The activation threshold is set at the factory and
cannot be adjusted.
Following activation, the over-temperature cut-out and alarm system operates in two sequential modes:
Mode 1: The over-temperature cut-out and alarm activates and cuts power to the heating elements and the
CO2 control valve. The condition is shown on the display by the message °Ctrip. When the chamber
temperature has fallen to the programmed setpoint, the system changes to Mode 2.
Mode 2: The control system then tries to maintain the chamber temperature at the programmed level by
switching the heating elements on and off, using an emergency control method that is, however, less
precise. The message TAL will flash on-screen to signal that an over-temperature fault occurred and the
incubator is being controlled by the emergency control system. This message cannot be cancelled via the
keypad.
Normal temperature control can be regained and the over-temperature cutout and alarm can be cancelled
by reprogramming the temperature, opening and closing the glass door, or switching the incubator off and
back on.
If the problem persists, the alarm will recur; if this happens, please contact your service representative or
your distributor immediately.
This alarm only occurs if the CO2 AutoZero cannot reference the sensor signal to atmosphere. If this is the
case, at the end of the autozero procedure, the following message will appear: CO2 AUTO ZERO FAI LED.
This means that the CO2 sensor is defective and requires replacement.
For easy reference, the following table provides an overview of all alarm messages that you may encounter
across both displays.
An external computer can be connected to the serial interface for data logging using optional BioCommand
SFI software, or by remote control with a communication program. Data from the incubator can be
downloaded for record keeping or validation documentation. The device can be controlled remotely and
operating parameters can be transmitted and recorded using third party software. Information on installing
and operating the communication program can be found in the corresponding software documentation.
The RS-232 interface port provides a connection for the incubator to a personal computer or terminal. This
allows the incubator’s operating status to be viewed on the screen.
The computer should be connected to a mains/power supply outlet as close as possible to the
incubator.
Prerequisites
• A PC or terminal, capable of RS-232 communication, with one serial com port free for this connection or
a serial interface box (see Main illustration on p. 9).
• A null-modem screened cable of suitable length. To ensure reliable communication, the cable should
not exceed 15 m (49 ft) in length. One end must have a 9-pin female D connector, to connect to the
incubator’s RS-232 port, and the other end must have either a 9-pin or 25-pin female D connector,
whichever mates to your PC or terminal (see Main illustration on p. 9).
• Suitable communications software, such as New Brunswick BioCommand SFI or HyperTerminal
(included with Windows NT, 95, 98, ME, 2000 and XP).
Operation - Galaxy 48 S
62 Galaxy® 48 R/48 S CO2 Incubators
English (EN)
1. Connect the null-modem cable to the RS-232 port at the rear of the incubator.
2. Connect the other end of the cable to either the 25-pin male D serial com port or the 9-pin male D serial
com port on the PC or terminal.
3. Turn on the computer and start the communications software.
4. Select an unused serial port to enable communications between the incubator and the PC or terminal. If
you know which port to use, continue to Step 5 and then skip to Step 7. If not, continue to Steps 5 and 6.
5. Using the communications software, select the unused port (COM1, COM2, COM3, etc.) then press the
ENTER key on the PC/terminal keyboard (refer to BioCommand SFI operating manual for instructions).
If you have successfully chosen an unused port, the message Type ? for help should appear on the
computer screen.
6. If you see any other message, or nothing happens, select the next port and press the ENTER key again.
If you still do not see the expected message, change the Flow Control setting from Hardware to None,
then try again.
7. When the connection between the incubator and PC/terminal has been successfully established, and the
message indicated in Step 5 appears, type (as prompted by the message onscreen) a ? in the
communications software, then press the ENTER key.
8. This menu will appear on the computer screen:
Operation - Galaxy 48 S
Galaxy® 48 R/48 S CO2 Incubators 63
English (EN)
This menu allows the incubator temperature, CO2 and alarm setpoints to be programmed remotely. A
running status report can be generated at user-defined intervals, and all reports can be sent to a printer. For
details on these features, consult the documentation supplied with your communications software.
Remote programming: all commands beginning with P can be used to program the incubator from the
remote computer. These commands are not case sensitive: lower case or upper case letters work exactly
the same way. The following are examples of how each of the above commands might be used:
In each case, the change is confirmed by a message from the incubator (e.g., Program Temperature 37.5 °C
OK, or Program Alarm Temperature High 38.0 °C OK, etc.).
Status commands: all commands beginning with S can be used to query the incubator from the remote
computer, and to display the current status of the incubator. As with the program commands, these
commands are not case sensitive: lower case or upper case letters work exactly the same way. The
following are examples of how each of the status commands might be used:
Other commands: As with the Program and Status commands, the V (Version number report) and ? (Help)
commands are not case sensitive. To use these two commands:
Operation - Galaxy 48 S
64 Galaxy® 48 R/48 S CO2 Incubators
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Alarm/Event Messages: a number of messages are preset to appear on the computer screen to inform you
of either an alarm condition or a certain event. The following table recaps those messages:
After displaying any of the above messages, a status report message relative to the alarm or event will be
displayed, e.g.:
7 Troubleshooting
7.1 O2 sensor
If the oxygen sensor fails suddenly, it is very likely that the sensor inlet membrane has become blocked by
condensation. This can be seen on the DATALOGGER screen as a sudden drop from the programmed value
to nearly zero.
1. Remove the hydrophobic filter holder assembly (see Replacing the filter disc on p. 48) by unscrewing it
(counter-clockwise).
2. Program the incubator for a temperature of at least 37 °C (or higher if you normally operate the
incubator at a higher temperature).
3. Close the door and allow the temperature to recover.
4. Reopen the door for 15 seconds to release any build-up of humidity.
5. Repeat steps 3 and 4 every 30 minutes while monitoring the DATALOGGER O2 Graph. The oxygen level
should recover after a few hours.
6. Leave the incubator for a few more hours to be ensure that the sensor membrane has thoroughly dried
out.
7. Replace the hydrophobic filter disc (see Replacing the filter disc on p. 48).
8. Re-humidify the incubator.
9. After 2 to 3 hours, carry out an OXYGEN SENSOR-REF TO ATMOSPHERE (see Referencing to atmosphere
on p. 45). When the referencing has been successfully completed, the incubator is ready for use.
Troubleshooting
66 Galaxy® 48 R/48 S CO2 Incubators
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Maintenance
Galaxy® 48 R/48 S CO2 Incubators 67
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8 Maintenance
8.1 Routine maintenance
8.1.1 General
To ensure that chamber conditions remain stable, minimize the length of time that the door is open. The
magnetic door catches are specifically designed to make door opening and closing as easy as possible.
When you open the door, wipe off any small drops of condensate that may have formed on the inner seal.
This will avoid a build-up of condensation.
If you are using the humidity tray for humidification, (see Using the humidity tray on p. 23).
1. Check that the temperature and CO2 levels are reading within specification.
2. Check the reserve pressure in the CO2 cylinder (normally 725 PSI (50 bar) when full). The design of the
incubator ensures very low consumption of CO2. During normal working conditions, a typical large
cylinder should last approximately 12 months (frequent door openings will deplete the supply more
rapidly). If there is a significant drop at the cylinder pressure of 725 PSI (50 bar), it means that the
cylinder is almost empty and should be replaced. Ensuring that there are no leaks at any of the
connections will ensure a greater lifetime to the CO2 supply and will help avoid accidentally running out
of CO2.
3. Any spills in the chamber should be cleaned immediately.
4. Check the DATALOGGER screen for any alarms or events that may have occurred overnight (48R only).
Use distilled water only in the humidity tray. Use of any other types of water including
deionized water will cause corrosion inside the incubator.
Refill the humidity tray with 0.5 liters of distilled water. The use of warm water will ensure a rapid return to
optimum chamber conditions.
We recommend routine replacement of the water in the humidity tray, and that you clean the tray at the
same time.
If required, you can take a sample of the gas inside the chamber using the CO2 sample port, and check it
using a CO2 gas analyzer (see CO2 Sampling with analyzer on p. 68).
Maintenance
68 Galaxy® 48 R/48 S CO2 Incubators
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Displayed chamber CO2 level will drop during sampling, but it will recover once the sampling
is complete. This is merely a sensor characteristic; the CO2 level in the chamber is actually not
affected.
We recommend that you perform a CO2 autozero prior to sampling. We also recommend that
you autozero the CO2 system at least once every 28 days to ensure that CO2 level is correct.
The CO2 sample port is located on the rear of the incubator, near the top (see Main illustration on p. 9).
• Turn off the CO2 gas by re-programming the setpoint for CO2 to 0.0 % to prevent CO2 from being
injected into the chamber and giving a false reading.
• A flow rate ≤ 0.5 liters/minute is used to take a sample.
• The door is kept closed.
• Reset the CO2 setpoint to the desired level after sampling.
We recommend that you perform a CO2 autozero prior to sampling. We also recommend that
you autozero the CO2 system at least once every 28 days to ensure that CO2 level is correct.
8.2 Cleaning
Switch the device off and pull the power plug out of the socket before beginning work.
1. Routinely clean the exterior of the incubator by wiping it over with a soft cloth, moistened with soapy
water.
2. Rinse the soap from the cloth in clean water, and wipe the exterior surfaces again.
Maintenance
Galaxy® 48 R/48 S CO2 Incubators 69
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8.3 Disinfection/Decontamination
Never use any of the following substances to clean the stainless steel, or damage will
result: Sodium Azide, Aqua Regia, Iodine, Ferric Chloride, Sulphuric Acid or Chlorine
based solvents.
It is very important to ensure that no liquid is spilled onto the white porous CO2 sensor
cover at the rear of the chamber. Failure to use the protective cover(s) could result in
damage to the sensor(s) and may affect your warranty.
Use distilled water only in the humidity tray. Use of any other types of water included
deionized water will cause corrosion inside the incubator.
The recommended disinfecting agent for use with the incubator is a solution of 70 % isopropanol
(isopropyl alcohol) and 30 % distilled water. Be sure to follow appropriate safety regulations while you are
using this solution.
To best protect yourself, your incubator and your work area, follow these instructions:
1. Program 0.0 % CO2 and switch off the incubator. Unplug the incubator from the mains/power supply.
2. Dampen a clean cloth with the alcohol solution and wipe down all external surfaces, taking care to keep
the alcohol solution from coming into contact with any mains/electrical outlets or assemblies.
3. Remove all of the shelves, the humidity tray, and the shelf racks.
4. Place the black protective cover over the CO2 sensor. Also protect any additional sensors, such as
Oxygen or Humidity, with the cover(s) supplied.
5. You can clean the humidity tray by rinsing it in sterile water, wiping it down with the alcohol solution,
and then rinsing it again with sterile water.
6. Wipe down the inside of the chamber with the alcohol/water solution, and leave it to dry completely.
Maintenance
70 Galaxy® 48 R/48 S CO2 Incubators
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7. Wipe the internal components of the chamber twice with the alcohol/water solution. Wipe off excess
liquid and leave it to dry completely.
8. Reassemble the shelf racks, shelves, and humidity tray before switching the incubator on. Wipe the
inner door seal with the alcohol solution, rinse and leave it to dry.
9. Ensure the protective cover(s) are removed from all sensor(s) and replaced in the holder for
safekeeping. Be very careful, as you remove the black CO2 sensor cover, not to accidentally remove the
white porous sensor cover. This must remain in place.
10. Refill the humidity tray (see Using the humidity tray on p. 23). When you reinstall it, ensure that the
humidity tray is pushed fully back.
11. Leave the incubator on for at least two hours (preferably overnight) to allow conditions to stabilize.
12. When the incubator has stabilized, carry out an autozero and reprogram the desired CO2 level. It may be
necessary to open the glass door briefly if, after performing an autozero, the CO2 level is too high.
If your incubator is supplied with the high temperature disinfection option, follow the guidelines outlined
(see Using the high temperature disinfection on p. 36) for information on how to correctly and safely operate
this option.
Transport, storage and disposal
Galaxy® 48 R/48 S CO2 Incubators 71
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Never try to lift the incubator by its door; this would cause permanent damage to the
incubator.
9.2 Disposal
In case the product is to be disposed of, the relevant legal regulations are to be observed.
Information on the disposal of electrical and electronic devices in the European Community:
Within the European Community, the disposal of electrical devices is regulated by national regulations
based on EU Directive 2012/19/EU pertaining to waste electrical and electronic equipment (WEEE).
According to these regulations, any devices supplied after August 13, 2005, in the business-to-business
sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. To
document this, they have been marked with the following identification:
Because disposal regulations may differ from one country to another within the EU, please contact your
supplier if necessary.
In Germany, this is mandatory from March 23, 2006. From this date, the manufacturer has to offer a
suitable method of return for all devices supplied after August 13, 2005. For all devices supplied before
August 13, 2005, the last user is responsible for the correct disposal.
9.3 Storage
10 Technical data
10.1 Weight/dimensions
10.1.1 Equipment dimensions
10.1.4 Shelves
10.3 Fuses
Fuse for Galaxy 48 R (120 V or 230 V) 5A
Fuse for Galaxy 48 S(120 V or 230 V) 4A
• Digital programming via microprocessor control in 0.1 °C increments. Measurement of chamber and
door temperature via 4 RT (Resistance Temperature curve) matched thermistors (sensitivity 0.01 °C)
• Adjustable independent control of door heater
• “Out of Limits” temperature protection system independent of microprocessor control.
If ambient temperature is close to the programmed value, control settings may need adjusting.
Please consult Eppendorf Service for instructions.
Solid-state infrared CO2 sensor operating independent of humidity. Programmable, fully automatic zeroing
function.
Range 0.2 - 20 %
Control ± 0.1 %
Stability ± 0.2 %
Uniformity ± 0.1 %
Recovery rate (see CO2 recovery on p. 75)
Gas connections 6 mm tubing
Required gas pressure 5 PSI (0.35 bar)
Air temperature 10 - 50 °C
After a door opening, it should be possible for the actual CO2 level to recover to around 4.8% in 2 to 2.5
minutes.
The displayed CO2 level takes 30 to 45 seconds more to catch up with the actual level. This is because it
takes approximately 45 seconds from the time the gas enters the chamber to fully mix and become uniform
inside the sensor.
This graph shows typical results for door openings between 10 and 45 seconds. The control system does
this is by speeding the sensor reaction time when the door is opened and then slowing it down to produce
a more accurate reading after the setpoint level is achieved.
Technical data
76 Galaxy® 48 R/48 S CO2 Incubators
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1 2 3
6
4
CO2 Level (% CO2)
0
-1 0 1 2 3 4 5 6
Time (Minutes)
Temperature:37.0 Deg. C
CO2 Level: 5.0% Actual CO2 Level
Humidified to 90 % RH Displayed CO2 Level
Door Opening 10 - 45 Secs Displayed and Actual CO2 Level
6 5 4
11 Ordering information
11.1 Accessories
For information regarding accessories not listed, contact a customer service representative.
Some option combinations are not possible, others may incur extra cost. Please inquire before ordering.
• O2 Control, 1 – 19 %
• O2 Control, 0.1 – 19 %
• O2 Control, 1 – 95 %
• High temperature disinfection
• Humidity alert and monitoring package
• Split inner door
• Building management system relay
• Internal power receptacle
• Upper stacking frame
• Lower and upper stacking frame
• Under-bench stand, 200 mm high with feet
Ordering information
78 Galaxy® 48 R/48 S CO2 Incubators
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Installation instructions for stacking stand kit
Galaxy® 48 R/48 S CO2 Incubators 79
English (EN)
1 2
4 3
From this point on, this incubator will be referred to as the bottom incubator.
Installation instructions for stacking stand kit
82 Galaxy® 48 R/48 S CO2 Incubators
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1 2
4 3
12.1.3 Specifications
Abb. 12-3: 2 Galaxy 48 R/48 S units stacked
3
4 5
13 Declaration of conformity
Abb. 13-1: 48 R declaration
Declaration of Conformity
The product named below fulfills the requirements of directives and standards listed. In the case of
unauthorized modifications to the product or an unintended use this declaration becomes invalid.
Product name:
Galaxy® 48 R
including accessories
Product type:
CO2 Incubator
2004/108/EC: EN 61326-1
2011/65/EU
Your local distributor: www.eppendorf.com/contact ISO 9001 ISO 13485 ISO 14001
Eppendorf AG · 22331 Hamburg · Germany
Certified Certified Certified
[email protected]
Eppendorf® and the Eppendorf Logo are registered trademarks of Eppendorf AG Hamburg/Germany.
All rights reserved incl. graphics and pictures. Copyright 2013 © by Eppendorf AG.
www.eppendorf.com
Declaration of Conformity
The product named below fulfills the requirements of directives and standards listed. In the case of
unauthorized modifications to the product or an unintended use this declaration becomes invalid.
Product name:
Galaxy® 48 S
including accessories
Product type:
CO2 Incubator
2004/108/EC: EN 61326-1
2011/65/EU
Your local distributor: www.eppendorf.com/contact ISO 9001 ISO 13485 ISO 14001
Eppendorf AG · 22331 Hamburg · Germany
Certified Certified Certified
[email protected]
Eppendorf® and the Eppendorf Logo are registered trademarks of Eppendorf AG Hamburg/Germany.
All rights reserved incl. graphics and pictures. Copyright 2013 © by Eppendorf AG.
www.eppendorf.com
T
Temperature sensor system alarms ......................59
Trademarks .............................................................2
Troubleshooting the O2 sensor .............................65
U
User requirement ..................................................15
User settings .........................................................26
Using this manual....................................................7
Utilities requirements............................................19
W
Warning, explanation of ..........................................7
Weekly checks .......................................................67
Working with cell culture ................................24, 56
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