MALDI Biotyper®

MBT Sepsityper® IVD Workflow

Direct pathogen identification from
positive blood culture bottles within 15-20 minutes
Innovation with Integrity

IVD
 When every
minute counts
Early identification and appropriate treatment
of blood stream infections and sepsis has
tremendous benefits for patient outcomes
and healthcare economy.
To address this topic, Bruker offers the
MBT Sepsityper solution, which is performed
in conjunction with the Bruker IVD MALDI
Biotyper system, saving precious time when
every minute counts.
The Sepsityper pathogen identification work-
flow reduces the turnaround time resulting
from traditional positive blood culture (PBC)
identification workflows by up to 48 hours.
Identification results can therefore be
reported significantly faster to ICU consul-
tants, compared to traditional methods.
Within an hour after the PBC alert, the ICU
consultant can be informed about a clear and
actionable pathogen identification result,
possibly leading to therapy optimization.

Fast pathogen identification, up to 48 hours quicker than 1

traditional methods
Urgent Blood Sample
from Patient
While taking even less than 10 minutes hands-on-time, the identifica-
tion result is available in only 15-20 minutes after the positive blood
culture (PBC) alert.
2
Accurate, specific and efficient
Positive Blood Culture
Pathogen identification, based on the extensive IVD MALDI Biotyper
library covering ~4,200 species of bacteria and yeast, yields actionable
results – all in one run.
3
Improving patient care and supporting antimicrobial Fast identification of
causative microorganism
stewardship within less than 10 minutes
hands-on-time
Fast pathogen identification enables early adjustment of antibiotic
therapy in sepsis patients.
4
Enables early adjustment
Cost-effective of antibiotic therapy

Earlier therapy change enables cost savings for the hospital by

improved patient management.

2
 Have your patient in focus

Be smart - be faster Be smart - be better

The Sepsityper solution helps identifying Implementing the Sepsityper workflow is not only resulting in saving
microorganisms from positive blood cultures valuable time, but also offers a higher identification success rate, lead-
considerably faster than the traditional work- ing to a better informed decision making when it comes to adjusting
flows based on short subculture. patient therapy.

Saving hours can make the difference when The Rapid Sepsityper workflow was compared to short subculturing
a patient is facing bacteraemia, sepsis and methods which represented the routine standard of three different
other conditions related to the presence of clinical microbiology laboratories. In this study, the Rapid workflow
microorganisms in the bloodstream. proved to be superior to the subculturing methods, while additionally
benefiting from a significantly shorter time-to-report. A higher rate of
correct identifications was observed, both in terms of total identifica-
tions as well as for each different microbial group.

Whereas the short subculture methods require just a minimal sample

handling, they are slower than the Rapid Sepsityper workflow, and are
not suitable for slow-growing and fastidious species.

With an investment of less than 10 minutes hands-on-time, the Rapid

Sepsityper workflow enables the identification of virtually every
species included in the MALDI Biotyper IVD-CE reference library.
Moreover, the samples can be processed in batches, speeding up the
workflow even more.

100
Rapid Sepsityper Short subculture
90

80
Percentage of correct identifications

70

60

50

40

30

20
For more details on the data shown, have
a look at our Application Note: 10

0
Gram-neg Gram-pos Anaerobes Yeasts

Comparison of Rapid Sepsityper results with those of short subculture for gram-
negatives, gram-positives, anaerobes and yeasts (% of correct ID)

App-Note 1889554 Arthur B. Pranada et al., Journal of Medical Microbiology 2022, Vol. 71, Issue 8.
MBT Sepsityper® IVD Kit
https://doi.org/10.1099/jmm.0.001571

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 Fast and easy workflow

MBT Sepsityper IVD Kit – enabling fast answers for

your patients
Transfer 1 mL of blood culture
fluid to a microcentrifuge tube The implementation of the Sepsityper ® solution in the laboratory
routine is highly justified by the benefit of successfully identifying
microorganisms only 15-20 minutes after a positive blood culture
1 min alert. Acting fast may change the patient’s outcome.
Moreover, the samples can be processed in batches, speeding up
the workflow even more.
Add 200 µL of lysis
buffer and mix The dedicated kit design and optimized formulation enable the
handling of slimy clots (e.g. Bacteroides fragilis), leading to a higher
identification success rate.
30 sec

MBT HT Sepsityper IVD Module – increasing the

Centrifuge 2 min and identification success rate
discard supernatant

2 min Microorganism identification from PBC does not only require special
sample preparation, but also a dedicated software providing adapted
data processing taking the complexity of a blood-derived sample into
account.
Add 1 mL of washing
buffer and mix Additionally, the software will provide a warning in case a mixed
culture is detected, with an indication of the detected species.

30 sec

Centrifuge 1 min and

discard supernatant

1 min

Direct Transfer or extended

Direct Transfer of the pellet,
add 1 µL of matrix

« Rapid Sepsityper Workflow »

If no ID:
ID on IVD MALDI Biotyper Standard extraction,
equipped with MBT HT Sepsityper spot 1 μL of extract onto the
IVD Module, covering ~4,200 species MALDI target, add 1 μL of
in one go matrix
ID on IVD MALDI Biotyper ®
10 min “Full Sepsityper Workflow”

4
Get even more out of your PBC sample

An early resistance warning system - aiding Fast phenotypic detection of

antimicrobial stewardship beta-lactamase activity - aiding
antimicrobial stewardship
Whenever the MALDI Biotyper routine identification workflow
results in successful identification of Klebsiella pneumoniae, The bacterial pellet resulting from the
Escherichia coli or Bacteroides fragilis, the optional MBT HT Sepsityper workflow can subsequently
Subtyping IVD Module automatically looks for specific resistance be used for phenotypic detection of
marker peaks in the identified mass spectrum. As a result, the carbapenemase and cephalosporinase
MBT HT Subtyping IVD Module quickly detects bla KPC expressing activity, within 60-90 minutes of the PBC
K. pneumoniae and E. coli, and distinguishes cfiA positive/ alert, by using the MBT STAR® -Carba IVD
negative B. fragilis strains, giving an early resistance warning Kit, respectively MBT STAR® -Cepha IVD
to the clinical microbiologist - without any additional work. Kit. The use of both kits is supported by
the dedicated MBT HT STAR® -BL IVD
Module. Contact us for more information!

IVD

HT

IVD
Covering thousands of species

HT

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 Clinical impact of a fast workflow

Improving patient outcomes

The Sepsityper solution shortens the identification time after a positive blood culture alert by up to 48 hours,
by eliminating the time-consuming step of culturing the microorganisms followed by fast identification using
MALDI-TOF mass spectrometry.

In combination with antibiotic stewardship programs, this rapid identification allows a much faster
optimization of antibiotic therapy in patients with sepsis, compared to conventional workflows.
Early results, which physicians can act upon to manage bloodstream infections, enforce the fight
against resistance, and improve patient outcomes.

Morning Batching

Standard Positive
Blood Culture Result ID next Day
Workflow

Actionable Result: 75% of

Sepsityper Result ID ICU informed
treatments can change*

Ward Rounds Sepsityper results communicated to ICU during ward round

››
am

am
am
am

10

12
11
9

* Stoneking LR, The Journal of Emergency Medicine, Vol. 44, No. 1, pp. 1–8, 2013​

Mr. Martin McGill

MSc, Lead specialist in Microbiology, University Hospital
Crosshouse, Scotland
“Blood cultures are far more important to patients than other
samples. In the case of a positive blood culture, time is freed
up for the Sepsityper Kit, all staff are fully trained and provided
protected time to run the Sepsityper test without interruptions.
Fast identification helps our biomedical scientists focus on
important samples and move only clinically significant samples
to AST”
“As for the Sepsityper, I think 4 hours is a long time to wait to
see if the patient has a Staphylococcus aureus or Group A strep;
an E. coli or Pseudomonas aeruginosa sepsis. For seriously ill
patients, every second counts.”

6
 Overall impact

Benefits of an early pathogen identification directly from positive blood cultures

 Earlier de-escalation and/or administration of targeted antimicrobial treatment helps improving the
patient outcome and supports antimicrobial stewardship
 Improving patient outcome may reduce the length of hospital stays and associated costs

 Early detection of contaminations avoids unnecessary antibiotic susceptibility testing,

saving time and costs

 Early detection of alert organisms helps improving overall hospital hygiene

 Clear identification results allow more efficient communication between microbiologists and treating physicians

Afternoon Batching

Result ID next Day

Actionable Result: 75% of

Result ID ICU informed
treatments can change*

Sepsityper results communicated to treating physician

››
pm

pm

pm

pm

pm
1

5
Prof. Dr. Alexia Verroken
University Hospital Saint-Luc, Brussels, Belgium

››
“Reducing turn-around-times of positive blood cultures with
MALDI-TOF MS ID accelerated prescription of targeted
antimicrobial treatment thereby potentially improving the
patient’s clinical outcome.”

Ms. Rushana A. Hussain

Section Manager Microbiology Dept., Royal Bolton Hospital
Foundation Trust, England
“The implementation of Sepsityper has resulted in a positive
impact on the direct management and treatment for patients.
Although it is difficult to calculate all the savings across the
patient pathway, the data clearly show that there are significant
financial benefits being achieved by the reduced length of
hospitalisation and reduction in antibiotics.”

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Order information

MBT Sepsityper IVD Kit

Part No. 1834338
Containing all reagents and consumables
required for microorganism isolation from
50 positive blood culture samples.

MBT HT Sepsityper IVD Module

Part No. 1877011
The MBT HT Sepsityper IVD Module
allows the definition of appropriate
samples as blood culture samples in the
MBT Compass HT IVD software (Part No
1877017).

No. 1814272 © 02-2023 Bruker Daltonics

Please contact your local representative for availability in your country.

Not for sale in the USA.

MALDI Biotyper ® is a registered trademark of the Bruker group of companies. Online information
Sepsityper ® is a registered trademark of the Bruker group of companies, bruker.com/microbiology
in the EU, Australia, China, Japan and Great Britain.

Bruker Daltonics GmbH & Co. KG

Bremen · Germany
Phone +49 (0) 421-2205-0

[email protected]