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Syllabus BSC Hons Microbiology
Syllabus BSC Hons Microbiology
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April 2015
Structure of B. Sc. Honours Microbiology under CBCS
Core Course
C-1: Introduction to Microbiology and Microbial Diversity
C-2: Bacteriology
C-3: Biochemistry
C-4: Virology
C-5: Microbial Physiology and Metabolism
C-6: Cell Biology
C-7: Molecular Biology
C-8: Microbial Genetics and Genomics
C-9: Environmental Microbiology
C-10: Food and Dairy Microbiology
C-11: Industrial Microbiology
C-12: Immunology
C-13: Medical Microbiology
C-14: Recombinant DNA Technology
Discipline Centric Elective (Any Four)
DSE-1: Bioinformatics
DSE-2: Microbial Biotechnology
DSE-3: Advances in Microbiology
DSE-4: Plant Pathology
DSE-5: Biomathematics and Biostatistics
DSE-6: Inheritance Biology
DSE-7: Microbes and Sustainable Development
DSE-8: IPR and Biosafety
DSE-9: Project Work
Generic Electives (Any Four)
GE-1: Introduction and Scope of Microbiology
GE-2: Bacteriology and Virology
GE-3: Microbial Metabolism
GE-4: Industrial and Food Microbiology
GE-5: Microbes in Environment
GE-6: Medical Microbiology and Immunology
GE-7: Genetic Engineering and Biotechnology
GE-8: Microbial Genetics and Molecular Biology
Ability Enhancement Compulsory Courses
AE-1: Environmental Sciences
AE-2: English/MIL Communication
Skill Enhancement Elective Courses (Any Two)
SE-1: Microbial Quality Control in Food and Pharmaceutical Industries
SE-2: Microbial Diagnosis in Health Clinics
SE-3: Biofertilizers and Biopesticides
SE-4: Food Fermentation Techniques
SE-5: Management of Human Microbial Diseases
SE-6: Microbiological analysis of air and water
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-1: INTRODUCTION TO MICROBIAL WORLD (THEORY)
SEMESTER –I
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Tortora GJ, Funke BR and Case CL. (2008). Microbiology: An Introduction. 9th edition. Pearson
Education
2. Madigan MT, Martinko JM, Dunlap PV and Clark DP. (2014). Brock Biology of Microorganisms.
14th edition. Pearson International Edition
3. Cappucino J and Sherman N. (2010). Microbiology: A Laboratory Manual. 9th edition. Pearson
Education Limited
4.Wiley JM, Sherwood LM and Woolverton CJ. (2013) Prescott’s Microbiology. 9th Edition. McGraw
Hill International.
5. Atlas RM. (1997). Principles of Microbiology. 2nd edition. WM.T.Brown Publishers.
6. Pelczar MJ, Chan ECS and Krieg NR. (1993). Microbiology. 5th edition. McGraw Hill Book
Company.
7. Stanier RY, Ingraham JL, Wheelis ML, and Painter PR. (2005). General Microbiology. 5th
edition. McMillan.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-2: BACTERIOLOGY (THEORY)
SEMESTER –I
TOTAL HOURS: 60 CREDITS: 4
1. Preparation of different media: synthetic media BG-11, Complex media-nutrient agar, McConkey
agar, EMB agar.
2. Simple staining
3. Negative staining
4. Gram’s staining
5. Acid fast staining-permanent slide only.
6. Capsule staining
7. Spore staining.
8. Isolation of pure cultures of bacteria by streaking method.
9. Preservation of bacterial cultures by various techniques.
10. Estimation of CFU count by spread plate method/pour plate method.
11. Motility by hanging drop method.
SUGGESTED READINGS
1. Atlas RM. (1997). Principles of Microbiology. 2nd edition. WM.T.Brown Publishers.
2. Black JG. (2008). Microbiology: Principles and Explorations. 7th edition. Prentice Hall
3. Madigan MT, and Martinko JM. (2014). Brock Biology of Micro-organisms. 14th edition. Parker J.
Prentice Hall International, Inc.
4. Pelczar Jr MJ, Chan ECS, and Krieg NR. (2004). Microbiology. 5th edition Tata McGraw Hill.
5. Srivastava S and Srivastava PS. (2003). Understanding Bacteria. Kluwer Academic Publishers,
Dordrecht
6. Stanier RY, Ingraham JL, Wheelis ML and Painter PR. (2005). General Microbiology. 5th edition
McMillan.
7. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An Introduction. 9th edition Pearson
Education.
8. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th edition.
McGraw Hill Higher Education.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-3: BIOCHEMISTRY (THEORY)
SEMESTER –II
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Campbell, MK (2012) Biochemistry, 7th ed., Published by Cengage Learning
2. Campbell, PN and Smith AD (2011) Biochemistry Illustrated, 4th ed., Published by Churchill
Livingstone
3. Tymoczko JL, Berg JM and Stryer L (2012) Biochemistry: A short course, 2nd ed., W.H.Freeman
4. Berg JM, Tymoczko JL and Stryer L (2011) Biochemistry, W.H.Freeman and Company
5. Nelson DL and Cox MM (2008) Lehninger Principles of Biochemistry, 5th Edition., W.H. Freeman
and Company,
6. Willey MJ, Sherwood, LM & Woolverton C J (2013) Prescott, Harley and Klein’s Microbiology
by. 9th Ed., McGrawHill
7. Voet,D. and Voet J.G (2004) Biochemistry 3rd edition, John Wiley and Sons,
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-4: VIROLOGY (THEORY)
SEMESTER –II
TOTAL HOURS: 60 CREDITS: 4
Unit 3 Viral Transmission, Salient features of viral nucleic acids and Replication
No. of Hours: 20
Modes of viral transmission: Persistent, non-persistent, vertical and horizontal
Salient features of viral Nucleic acid : Unusual bases (TMV,T4 phage), overlapping genes (ɸX174,
Hepatitis B virus), alternate splicing (HIV), terminal redundancy (T4 phage), terminal cohesive ends
(lambda phage), partial double stranded genomes (Hepatitis B), long terminal repeats (retrovirus),
segmented (Influenza virus), and non-segmented genomes (picornavirus), capping and tailing (TMV)
Viral multiplication and replication strategies: Interaction of viruses with cellular receptors and entry
of viruses. Replication strategies of viruses as per Baltimore classification (phi X 174, Retroviridae,
Vaccinia, Picorna) , Assembly, maturation and release of virions
1. Study of the structure of important animal viruses (rhabdo, influenza, paramyxo hepatitis B and
retroviruses) using electron micrographs
2. Study of the structure of important plant viruses (caulimo, Gemini, tobacco ring spot, cucumber
mosaic and alpha-alpha mosaic viruses) using electron micrographs
3. Study of the structure of important bacterial viruses (ɸX 174, T4, λ) using electron micrograph.
4. Isolation and enumeration of bacteriophages (PFU) from water/sewage sample using double agar
layer technique
5. Studying isolation and propagation of animal viruses by chick embryo technique
6. Study of cytopathic effects of viruses using photographs
7. Perform local lesion technique for assaying plant viruses.
SUGGESTED READING
1. Dimmock, NJ, Easton, AL, Leppard, KN (2007). Introduction to Modern Virology. 6th edition,
Blackwell Publishing Ltd.
2. Carter J and Saunders V (2007). Virology: Principles and Applications. John Wiley and Sons.
3. Flint SJ, Enquist, LW, Krug, RM, Racaniello, VR, Skalka, AM (2004). Principles of Virology,
Molecular biology, Pathogenesis and Control. 2nd edition. ASM press Washington DC.
4. Levy JA, Conrat HF, Owens RA. (2000). Virology. 3rd edition. Prentice Hall publication, New
Jersey.
5. Wagner EK, Hewlett MJ. (2004). Basic Virology. 2nd edition. Blackwell Publishing.
6. Mathews. (2004). Plant Virology. Hull R. Academic Press, New York.
7. Nayudu MV. (2008). Plant Viruses. Tata McGraw Hill, India.
8. Bos L. (1999) Plant viruses-A text book of plant virology by. Backhuys Publishers.
9. Versteeg J. (1985). A Color Atlas of Virology. Wolfe Medical Publication.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-5: MICROBIAL PHYSIOLOGY AND METABOLISM (THEORY)
SEMESTER –III
TOTAL HOURS: 60 CREDITS: 4
Unit 1 Microbial Growth and Effect of Environment on Microbial Growth No. of Hours: 12
Definitions of growth, Batch culture, Continuous culture, generation time and specific growth rate
Temperature and temperature ranges of growth
pH and pH ranges of growth
Effect of solute and water activity on growth
Effect of oxygen concentration on growth
Nutritional categories of microorganisms
1. Study and plot the growth curve of E. coli by tubidiometric and standard plate count methods.
2. Calculations of generation time and specific growth rate of bacteria from the graph plotted with the
given data
3. Effect of temperature on growth of E. coli
4. Effect of pH on growth of E. coli
5. Demonstration of alcoholic fermentation
6. Demonstration of the thermal death time and decimal reduction time of E. coli.
SUGGESTED READINGS
1. Madigan MT, and Martinko JM (2014). Brock Biology of Microorganisms. 14th edition. Prentice
Hall International Inc.
2. Moat AG and Foster JW. (2002). Microbial Physiology. 4th edition. John Wiley & Sons
3. Reddy SR and Reddy SM. (2005). Microbial Physiology. Scientific Publishers India
4. Gottschalk G. (1986). Bacterial Metabolism. 2nd edition. Springer Verlag
6. Stanier RY, Ingrahm JI, Wheelis ML and Painter PR. (1987). General Microbiology. 5th edition,
McMillan Press.
7. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th edition.
McGraw Hill Higher Education.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-6: CELL BIOLOGY (THEORY)
SEMESTER –III
TOTAL HOURS: 60 CREDITS: 4
Unit 5 Cell Cycle, Cell Death and Cell Renewal No. of Hours: 12
Eukaryotic cell cycle and its regulation, Mitosis and Meiosis
Development of cancer, causes and types
Programmed cell death
Stem cells
Embryonic stem cell, induced pleuripotent stem cells
SUGGESTED READING
1. Hardin J, Bertoni G and Kleinsmith LJ. (2010). Becker’s World of the Cell. 8th edition. Pearson.
2. Karp G. (2010) Cell and Molecular Biology: Concepts and Experiments. 6th edition. John Wiley &
Sons. Inc.
3. De Robertis, EDP and De Robertis EMF. (2006). Cell and Molecular Biology. 8th edition. Lipincott
Williams and Wilkins, Philadelphia.
4. Cooper, G.M. and Hausman, R.E. (2009). The Cell: A Molecular Approach. 5th Edition. ASM
Press & Sunderland, Washington, D.C.; Sinauer Associates, MA.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-7: MOLECULAR BIOLOGY (THEORY)
SEMESTER –III
TOTAL HOURS: 60 CREDITS: 4
1. Study of different types of DNA and RNA using micrographs and model / schematic
representations
2. Study of semi-conservative replication of DNA through micrographs / schematic
representations
3. Isolation of genomic DNA from E. coli
4. Estimation of salmon sperm / calf thymus DNA using colorimeter (diphenylamine
reagent) or UV spectrophotometer (A260 measurement)
5. Estimation of RNA using colorimeter (orcinol reagent) or UV spectrophotometer
(A260 measurement)
6. Resolution and visualization of DNA by Agarose Gel Electrophoresis.
7. Resolution and visualization of proteins by Polyacrylamide Gel Electrophoresis (SDS-PAGE).
SUGGESTED READINGS
1. Watson JD, Baker TA, Bell SP, Gann A, Levine M and Losick R (2008) Molecular Biology of the
Gene, 6th edition, Cold Spring Harbour Lab. Press, Pearson Publication
2. Becker WM, Kleinsmith LJ, Hardin J and Bertoni GP (2009) The World of the Cell, 7th edition,
Pearson Benjamin Cummings Publishing, San Francisco
3. De Robertis EDP and De Robertis EMF (2006) Cell and Molecular Biology, 8th edition. Lippincott
Williams and Wilkins, Philadelphia
4. Karp G (2010) Cell and Molecular Biology: Concepts and Experiments, 6th edition, John Wiley &
Sons. Inc.
5. Sambrook J and Russell DW. (2001). Molecular Cloning: A Laboratory Manual. 4th Edition, Cold
Spring Harbour Laboratory press.
6. Krebs J, Goldstein E, Kilpatrick S (2013). Lewin’s Essential Genes, 3rd Ed., Jones and Bartlett
Learning
7. Gardner EJ, Simmons MJ, Snustad DP (2008). Principles of Genetics. 8th Ed. Wiley-India
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-8: MICROBIAL GENETICS AND GENOMICS (THEORY)
SEMESTER –IV
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Klug WS, Cummings MR, Spencer, C, Palladino, M (2011). Concepts of Genetics, 10th Ed.,
Benjamin Cummings
2. Krebs J, Goldstein E, Kilpatrick S (2013). Lewin’s Essential Genes, 3rd Ed., Jones and Bartlett
Learning
3. Pierce BA (2011) Genetics: A Conceptual Approach, 4th Ed., Macmillan Higher Education
Learning
4. Watson JD, Baker TA, Bell SP et al. (2008) Molecular Biology of the Gene, 6th Ed., Benjamin
Cummings
5. Gardner EJ, Simmons MJ, Snustad DP (2008). Principles of Genetics. 8th Ed. Wiley-India
6. Russell PJ. (2009). i Genetics- A Molecular Approach. 3rd Ed, Benjamin Cummings
7. Sambrook J and Russell DW. (2001). Molecular Cloning: A Laboratory Manual. 4th Edition, Cold
Spring Harbour Laboratory press.
8. Maloy SR, Cronan JE and Friefelder D(2004) Microbial Genetics 2nd EDITION., Jones and Barlett
Publishers
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-9: ENVIRONMENTAL MICROBIOLOGY (THEORY)
SEMESTER –IV
TOTAL HOURS: 60 CREDITS: 4
1. Analysis of soil - pH, moisture content, water holding capacity, percolation, capillary action.
2. Isolation of microbes (bacteria & fungi) from soil (28ºC & 45ºC ).
3. Isolation of microbes (bacteria & fungi) from rhizosphere and rhizoplane.
4. Assessment of microbiological quality of water.
5. Determination of BOD of waste water sample.
6. Study the presence of microbial activity by detecting (qualitatively) enzymes (dehydrogenase,
amylase, urease) in soil.
7. Isolation of Rhizobium from root nodules.
SUGGESTED READINGS
1. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications. 4th edition.
Benjamin/Cummings Science Publishing, USA
2. Madigan MT, Martinko JM and Parker J. (2014). Brock Biology of Microorganisms. 14th edition.
Pearson/ Benjamin Cummings
3. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2nd edition, Academic
Press
4. Okafor, N (2011). Environmental Microbiology of Aquatic & Waste systems. 1st edition, Springer,
New York
5. Singh A, Kuhad, RC & Ward OP (2009). Advances in Applied Bioremediation. Volume 17,
Springer-Verlag, Berlin Hedeilberg
6. Barton LL & Northup DE (2011). Microbial Ecology. 1st edition, Wiley Blackwell, USA
Campbell RE. (1983). Microbial Ecology. Blackwell Scientific Publication, Oxford, England.
7. Coyne MS. (2001). Soil Microbiology: An Exploratory Approach. Delmar Thomson Learning.
8. Lynch JM & Hobbie JE. (1988). Microorganisms in Action: Concepts & Application in Microbial
Ecology. Blackwell Scientific Publication, U.K.
9. Martin A. (1977). An Introduction to Soil Microbiology. 2nd edition. John Wiley & Sons Inc. New
York & London.
10. Stolp H. (1988). Microbial Ecology: Organisms Habitats Activities. Cambridge University Press,
Cambridge, England.
11. Subba Rao NS. (1999). Soil Microbiology. 4th edition. Oxford & IBH Publishing Co. New Delhi.
12. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th edition.
McGraw Hill Higher Education.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-10: FOOD AND DAIRY MICROBIOLOGY (THEORY)
SEMESTER –IV
TOTAL HOURS: 60 CREDITS: 4
Unit 5 Food borne diseases (causative agents, foods involved, symptoms and preventive
measures) No. of Hours: 10
Food intoxications: Staphylococcus aureus, Clostridium botulinum and mycotoxins;
Food infections: Bacillus cereus, Vibrio parahaemolyticus, Escherichia coli, Salmonellosis,
Shigellosis, Yersinia enterocolitica, Listeria monocytogenes and Campylobacter jejuni
Unit 7 Cultural and rapid detection methods of food borne pathogens in foods and introduction
to predictive microbiology. No. of Hours: 5
SUGGESTED READINGS
1. Adams MR and Moss MO. (1995). Food Microbiology. 4th edition, New Age International (P)
Limited Publishers, New Delhi, India.
2. Banwart JM. (1987). Basic Food Microbiology. 1st edition. CBS Publishers and Distributors,
Delhi, India.
3. Davidson PM and Brannen AL. (1993). Antimicrobials in Foods. Marcel Dekker, New York.
4. Dillion VM and Board RG. (1996). Natural Antimicrobial Systems and Food Preservation. CAB
International, Wallingford, Oxon.
5. Frazier WC and Westhoff DC. (1992). Food Microbiology. 3rd edition. Tata McGraw-Hill
Publishing Company Ltd, New Delhi, India.
6. Gould GW. (1995). New Methods of Food Preservation. Blackie Academic and Professional,
London.
7. Jay JM, Loessner MJ and Golden DA. (2005). Modern Food Microbiology. 7th edition, CBS
Publishers and Distributors, Delhi, India.
8. Lund BM, Baird Parker AC, and Gould GW. (2000). The Microbiological Safety and Quality of
Foods. Vol. 1-2, ASPEN Publication, Gaithersberg, MD.
9. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An Introduction. 9th edition. Pearson
Education.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-11: INDUSTRIAL MICROBIOLOGY (THEORY)
SEMESTER –V
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READINGS
1. Patel A.H. (1996). Industrial Microbiology. 1st edition, Macmillan India Limited
2. Okafor N. (2007). Modern Industrial Microbiology and Biotechnology. 1st edition. Bios Scientific
Publishers Limited. USA
3. Waites M.J., Morgan N.L., Rockey J.S. and Higton G. (2001). Industrial Microbiology: An
Introduction. 1st edition. Wiley – Blackwell
4. Glaze A.N. and Nikaido H. (1995). Microbial Biotechnology: Fundamentals of Applied
Microbiology. 1st edition. W.H. Freeman and Company
5. Casida LE. (1991). Industrial Microbiology. 1st edition. Wiley Eastern Limited.
6. Crueger W and Crueger A. (2000). Biotechnology: A textbook of Industrial Microbiology. 2nd
edition. Panima Publishing Co. New Delhi.
7. Stanbury PF, Whitaker A and Hall SJ. (2006). Principles of Fermentation Technology. 2nd edition,
Elsevier Science Ltd.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-12: IMMUNOLOGY (THEORY)
SEMESTER –V
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READINGS
1. Abbas AK, Lichtman AH, Pillai S. (2007). Cellular and Molecular Immunology. 6th edition
Saunders Publication, Philadelphia.
2. Delves P, Martin S, Burton D, Roitt IM. (2006). Roitt’s Essential Immunology.11th edition Wiley-
Blackwell Scientific Publication, Oxford.
3. Goldsby RA, Kindt TJ, Osborne BA. (2007). Kuby’s Immunology. 6th edition W.H. Freeman and
Company, New York.
4. Murphy K, Travers P, Walport M. (2008). Janeway’s Immunobiology. 7th edition Garland Science
Publishers, New York.
5. Peakman M, and Vergani D. (2009). Basic and Clinical Immunology. 2nd edition Churchill
Livingstone Publishers, Edinberg.
6. Richard C and Geiffrey S. (2009). Immunology. 6th edition. Wiley Blackwell Publication.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-13: MEDICAL MICROBIOLOGY (THEORY)
SEMESTER –VI
TOTAL HOURS: 60 CREDITS: 4
Unit 1 Normal microflora of the human body and host pathogen interaction
No. of Hours: 8
Normal microflora of the human body: Importance of normal microflora, normal microflora of skin,
throat, gastrointestinal tract, urogenital tract
Host pathogen interaction: Definitions - Infection, Invasion, Pathogen, Pathogenicity, Virulence,
Toxigenicity, Carriers and their types, Opportunistic infections, Nosocomial infections. Transmission
of infection, Pathophysiologic effects of LPS
Unit 6 Antimicrobial agents: General characteristics and mode of action No. of Hours: 8
Antibacterial agents: Five modes of action with one example each: Inhibitor of nucleic acid synthesis;
Inhibitor of cell wall synthesis; Inhibitor of cell membrane function; Inhibitor of protein synthesis;
Inhibitor of metabolism
Antifungal agents: Mechanism of action of Amphotericin B, Griseofulvin
Antiviral agents: Mechanism of action of Amantadine, Acyclovir, Azidothymidine
Antibiotic resistance, MDR, XDR, MRSA, NDM-1
SUGGESTED READING
1. Ananthanarayan R. and Paniker C.K.J. (2009) Textbook of Microbiology. 8th edition, University
Press Publication
2. Brooks G.F., Carroll K.C., Butel J.S., Morse S.A. and Mietzner, T.A. (2013) Jawetz, Melnick and
Adelberg’s Medical Microbiology. 26th edition. McGraw Hill Publication
3. Goering R., Dockrell H., Zuckerman M. and Wakelin D. (2007) Mims’ Medical Microbiology. 4th
edition. Elsevier
4. Willey JM, Sherwood LM, and Woolverton CJ. (2013) Prescott, Harley and Klein’s Microbiology.
9th edition. McGraw Hill Higher Education
5. Madigan MT, Martinko JM, Dunlap PV and Clark DP. (2014). Brock Biology of Microorganisms.
14th edition. Pearson International Edition
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-14: RECOMBINANT DNA TECHNOLOGY (THEORY)
SEMESTER –VI
TOTAL HOURS: 60 CREDITS: 4
Unit 5 Construction and Screening of Genomic and cDNA libraries No. of Hours: 6
Genomic and cDNA libraries: Preparation and uses, Screening of libraries: Colony hybridization and
colony PCR, Chromosome walking and chromosome jumping
SUGGESTED READING
1. Brown TA. (2010). Gene Cloning and DNA Analysis. 6th edition. Blackwell Publishing, Oxford,
U.K.
2. Clark DP and Pazdernik NJ. (2009). Biotechnology: Applying the Genetic Revolution. Elsevier
Academic Press, USA
3. Primrose SB and Twyman RM. (2006). Principles of Gene Manipulation and Genomics, 7th edition.
Blackwell Publishing, Oxford, U.K.
4. Sambrook J and Russell D. (2001). Molecular Cloning-A Laboratory Manual. 3rd edition. Cold
Spring Harbor Laboratory Press
5. Wiley JM, Sherwood LM and Woolverton CJ. (2008). Prescott, Harley and Klein’s Microbiology.
McGraw Hill Higher Education
6. Brown TA. (2007). Genomes-3. Garland Science Publishers
7. Primrose SB and Twyman RM. (2008). Genomics: Applications in human biology. Blackwell
Publishing, Oxford, U.K.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-1: BIOINFORMATICS (THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Saxena Sanjay (2003) A First Course in Computers, Vikas Publishing House
2. Pradeep and Sinha Preeti (2007) Foundations of Computing, 4th ed., BPB Publications
3. Lesk M.A.(2008) Introduction to Bioinformatics . Oxford Publication, 3rd International Student
Edition
4. Rastogi S.C., Mendiratta N. and Rastogi P. (2007) Bioinformatics: methods and applications,
genomics, proteomics and drug discovery, 2nd ed. Prentice Hall India Publication
5. Primrose and Twyman (2003) Principles of Genome Analysis & Genomics. Blackwell
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-2: MICROBIAL BIOTECHNOLOGY (THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Ratledge, C and Kristiansen, B. (2001). Basic Biotechnology, 2nd Edition, Cambridge University
Press.
2. Demain, A. L and Davies, J. E. (1999). Manual of Industrial Microbiology and Biotechnology, 2nd
Edition, ASM Press.
3. Swartz, J. R. (2001). Advances in Escherichia coli production of therapeutic proteins. Current
Opinion in Biotechnology, 12, 195–201.
4. Prescott, Harley and Klein’s Microbiology by Willey JM, Sherwood LM, Woolverton CJ (2014),
9th edition, Mc Graw Hill Publishers.
5. Gupta PK (2009) Elements of Biotechnology 2nd edition, Rastogi Publications,
6. Glazer AN and Nikaido H (2007) Microbial Biotechnology, 2nd edition, Cambridge University
Press
7. Glick BR, Pasternak JJ, and Patten CL (2010) Molecular Biotechnology 4th edition, ASM Press,
8. Stanbury PF, Whitaker A, Hall SJ (1995) Principles of Fermentation Technology 2nd edition.,
Elsevier Science
9. Crueger W, Crueger A (1990) Biotechnology: A text Book of Industrial Microbiology 2nd edition
Sinauer associates, Inc.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-3: ADVANCES IN MICROBIOLOGY (THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Fraser CM, Read TD and Nelson KE. Microbial Genomes, 2004, Humana Press
2. Miller RV and Day MJ. Microbial Evolution- Gene establishment, survival and exchange, 2004,
ASM Press
3. Bull AT. Microbial Diversity and Bioprospecting, 2004, ASM Press
4. Sangdun C. Introduction to Systems Biology, 2007, Humana Press
5. Klipp E, Liebermeister W. Systems Biology – A Textbook, 2009, Wiley –VCH Verlag
6. Caetano-Anolles G. Evolutionary Genomics and Systems Biology, 2010, John Wiley and Sons
7. Madigan MT, Martink JM, Dunlap PV and Clark DP (2014) Brook’s Biology of Microorganisms,
14th edition, Pearson-Bejamin Cummings
8. Wilson BA, Salyers AA Whitt DD and Winkler ME (2011)Bacterial Pathogenesis- A molecular
Approach, 3rd edition, ASM Press,
9. Bouarab K, Brisson and Daayf F (2009) Molecular Plant-Microbe interaction CAB International
10. Voit EO (2012) A First Course in Systems Biology, Ist edition,Garland Science
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-4: PLANT PATHOLOGY (THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READINGS
1. Agrios GN. (2006). Plant Pathology. 5th edition. Academic press, San Diego,
2. Lucas JA. (1998). Plant Pathology and Plant Pathogens. 3rd edition. Blackwell Science, Oxford.
3. Mehrotra RS. (1994). Plant Pathology. Tata McGraw-Hill Limited.
4. Rangaswami G. (2005). Diseases of Crop Plants in India. 4th edition. Prentice Hall of India Pvt.
Ltd., New Delhi.
5. Singh RS. (1998). Plant Diseases Management. 7th edition. Oxford & IBH, New Delhi.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-5: BIOMATHEMATICS AND BIOSTATISTICS (THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
Sets. Functions and their graphs : polynomial, sine, cosine, exponential and logarithmic functions.
Motivation and illustration for these functions through projectile motion, simple pendulum, biological
rhythms, cell division, muscular fibres etc.
Simple observations about these functions like increasing, decreasing and, periodicity.
Sequences to be introduced through the examples arising in Science beginning with finite sequences,
followed by concepts of recursion and difference equations. For instance, the Fibonacci sequence
arising from branching habit of trees and breeding habit of rabbits. Intuitive idea of algebraic
relationships and convergence.
Infinite Geometric Series. Series formulas for ex, log (1+x), sin x, cos x. Step function. Intuitive idea
of discontinuity, continuity and limits.
Differentiation. Conception to be motivated through simple concrete examples as given above from
Biological and Physical Sciences. Use of methods of differentiation like Chain rule, Product rule and
Quotient rule. Second order derivatives of above functions.
Integration as reverse process of differentiation.
Integrals of the functions introduced above. Differential Equations of first order, Linear Differential
Equations.
Points in plane and space and coordinate form. Examples of matrices arising in Biological Sciences
and Biological networks. Sum and Produce of matrices upto order 3.
Unit 2 No of Hours: 30
Measures of central tendency, Measures of dispersion; skewness, kurtosis; Elementary Probability and
basic laws; Discrete and Continuous Random variable, Mathematical Expectation; Curve Fitting;
Correlation and Regression. Emphasis on examples from Biological Sciences;
Mean and Variance of Discrete and Continuous Distributions namely Binomial, Poisson, Geometric,
Weibull, Logistic and Normal distribution. Fitting of Distributions;
Statistical methods: Scope of statistics: utility and misuse. Principles of statistical analysis of
biological data. Sampling parameters. Difference between sample and Population, Sampling Errors,
Censoring, difference between parametric and non-parametric statistics;
Sampling Distributions, Standard Error, Testing of Hypothesis, Level of Significance and Degree of
Freedom;
Large Sample Test based on Normal Distribution, Small sample test based on t-test, Z- test and F
test; Confidence Interval; Distribution-free test - Chi-square test;
Basic introduction to Multivariate statistics, etc.
SUGGESTED READINGS
1. H. S. Bear: Understanding Calculus, John Wiley and Sons (Second Edition); 2003.
2. E. Batschelet : Introduction to Mathematics for Life Scientists,Springer Verlag, International
Student Edition, Narosa Publishing House, New Delhi (1971, 1975)
3. A. Edmondson and D. Druce : Advanced Biology Statistics, Oxford University Press; 1996.
4. W. Danial : Biostatistics : A foundation for Analysis in Health Sciences, John Wiley and Sons Inc;
2004.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-6: INHERITANCE BIOLOGY (THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Gardner EJ, Simmons MJ, Snustad DP (2008). Principles of Genetics. 8th Ed. Wiley-India
2. Snustad DP, Simmons MJ (2011). Principles of Genetics. 6th Ed. John Wiley and Sons Inc.
3. Weaver RF, Hedrick PW (1997). Genetics. 3rd Ed. McGraw-Hill Education
4. Klug WS, Cummings MR, Spencer CA, Palladino M (2012). Concepts of Genetics. 10th Ed.
Benjamin Cummings
5. Griffith AJF, Wessler SR, Lewontin RC, Carroll SB. (2007). Introduction to Genetic Analysis. 9th
Ed. W.H.Freeman and Co., New York
6. Hartl DL, Jones EW (2009). Genetics: Analysis of Genes and Genomes. 7th Ed, Jones and Bartlett
Publishers
7. Russell PJ. (2009). i Genetics - A Molecular Approach. 3rd Ed, Benjamin Cummings
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-7: MICROBES IN SUSTAINABLE AGRICULTURE AND DEVELOPMENT
(THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READINGS
1. Agrios GN. (2006). Plant Pathology. 5th edition. Academic press, San Diego,
2. Singh RS. (1998). Plant Diseases Management. 7th edition. Oxford & IBH, New Delhi.
3. Glick BR, Pasternak JJ, and Patten CL (2010) Molecular Biotechnology 4th edition, ASM Press,
4. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications. 4th edition.
Benjamin/Cummings Science Publishing, USA
5. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2nd edition, Academic
Press
6. Barton LL & Northup DE (2011). Microbial Ecology. 1st edition, Wiley Blackwell, USA
Campbell RE. (1983). Microbial Ecology. Blackwell Scientific Publication, Oxford, England.
7. Coyne MS. (2001). Soil Microbiology: An Exploratory Approach. Delmar Thomson Learning.
8. Altman A (1998). Agriculture Biotechnology, ist edition, Marcel decker Inc.
9. Mahendra K. Rai (2005). Hand book of Microbial biofertilizers, The Haworth Press, Inc. New
York.
10. Reddy, S.M. et. al. (2002). Bioinoculants for sustainable agriculture and forestry, Scientific
Publishers.
11. Saleem F and Shakoori AR (2012) Development of Bioinsecticide, Lap Lambert Academic
Publishing GmbH KG
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-8: BIOSAFETY AND IPR (THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
Unit 1 No of Hours: 8
Biosafety: Introduction; biosafety issues in biotechnology; Biological Safety Cabinets & their types;
Primary Containment for Biohazards; Biosafety Levels of Specific Microorganisms
Unit 2 No of Hours: 12
Biosafety Guidelines: Biosafety guidelines and regulations (National and International);
GMOs/LMOs- Concerns and Challenges; Role of Institutional Biosafety Committees, RCGM, GEAC
etc. for GMO applications in food and agriculture; Environmental release of GMOs; Risk Analysis;
Risk Assessment; Risk management and communication; Overview of International Agreements -
Cartagena Protocol.
Unit 3 No of Hours: 4
AERB/RSD/RES guidelines for using radioisotopes in laboratories and precautions.
Unit 4 No of Hours: 12
Introduction to Intellectual Property: Patents, Types, Trademarks, Copyright & Related Rights,
Industrial Design and Rights, Traditional Knowledge, Geographical Indications- importance of IPR –
patentable and non patentables – patenting life – legal protection of biotechnological inventions –
world intellectual property rights organization (WIPO).
Unit 5 No of Hours: 12
Grant of Patent and Patenting Authorities: Types of patent applications: Ordinary, PCT, Conventional,
Divisional and Patent of Addition; An introduction to Patent Filing Procedures; Patent licensing and
agreement; Patent infringement- meaning, scope, litigation, case studies, Rights and Duties of patent
owner.
Unit 6 No of Hours: 12
Agreements and Treaties: GATT, TRIPS Agreements; Role of Madrid Agreement; Hague Agreement;
WIPO Treaties; Budapest Treaty on international recognition of the deposit of microorganisms;
UPOV & Brene conventions; Patent Co-operation Treaty (PCT); Indian Patent Act 1970 & recent
amendments.
Suggested Reading
1. Bare Act, 2007.Indian Patent Act 1970 Acts & Rules, Universal Law Publishing Co. Pvt. Ltd., New
Delhi.
2. Kankanala C (2007). Genetic Patent Law & Strategy, 1st Edition, Manupatra Information Solution
Pvt. Ltd. New Delhi.
3. Mittal, D.P. (1999). Indian Patents Law, Taxmann, Allied Services (p) Ltd.
4. Singh K K (2015). Biotechnology and Intelectual Property Rights: Legal and Social Impliocations,
Springer India.
5. Goel D & Prashar S (2013). IPR, Biosafety and Bioethics. Pearson
6. Senthil Kumar Sadhasivam and Mohammed Jaabir, M. S. 2008. IPR, Biosafety and biotechnology
Management. Jasen Publications, Tiruchirappalli, India.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
GE-1: INTRODUCTION AND SCOPE OF MICROBIOLOGY (THEORY)
SEMESTER –I
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Tortora GJ, Funke BR and Case CL. (2008). Microbiology: An Introduction. 9th edition. Pearson
Education
2. Madigan MT, Martinko JM, Dunlap PV and Clark DP. (2014). Brock Biology of Microorganisms.
14th edition. Pearson International Edition
3. Cappucino J and Sherman N. (2010). Microbiology: A Laboratory Manual. 9th edition. Pearson
Education Limited
4.Wiley JM, Sherwood LM and Woolverton CJ. (2013) Prescott’s Microbiology. 9th Edition. McGraw
Hill International.
5. Atlas RM. (1997). Principles of Microbiology. 2nd edition. WM.T.Brown Publishers.
6. Pelczar MJ, Chan ECS and Krieg NR. (1993). Microbiology. 5th edition. McGraw Hill Book
Company.
7. Stanier RY, Ingraham JL, Wheelis ML, and Painter PR. (2005). General Microbiology. 5th
edition. McMillan.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
GE-2: BACTERIOLOGY AND VIROLOGY (THEORY)
SEMESTER – II
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Atlas RM. (1997). Principles of Microbiology. 2nd edition. WM.T.Brown Publishers
2. Madigan MT, Martinko JM, Dunlap PV and Clark DP (2014). Brock Biology of Micro-organisms.
14th edition. Pearson Education, Inc.
3. Stanier RY, Ingraham JL, Wheelis ML and Painter PR. (2005). General Microbiology.
5th edition. McMillan
4. Carter J and Saunders V(2007). Virology; principles and Applications. John Wiley and Sons
5. Flint SJ, Enquist, LW, Krug, RM, Racaniello, VR Skalka, AM (2004) Principles of Virology,
Molecular Biology, Pathogenesis and Control.2nd edition.ASM Press
6. Shors Teri (2013) Understanding Viruses 2nd edition Jones and Bartlett Learning Burlington USA
7. Pelczar Jr MJ, Chan ECS, and Krieg NR. (2004). Microbiology. 5th edition Tata McGraw Hill.
8. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An Introduction. 9th edition Pearson
Education.
9. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th edition.
McGraw Hill Higher Education.
10. Dimmock, NJ, Easton, AL, Leppard, KN (2007). Introduction to Modern Virology. 6th edition,
Blackwell Publishing Ltd.
11. Cann AJ (2012) Principles of Molecular Virology, Academic Press Oxford UK
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
GE-3: MICROBIAL METABOLISM (THEORY)
SEMESTER – III
TOTAL HOURS: 60 CREDITS: 4
Unit 1 Microbial Growth and Effect of Environment on Microbial Growth No. of Hours: 12
Definitions of growth, Batch culture, Continuous culture, generation time and specific growth rate
Temperature and temperature ranges of growth
pH and pH ranges of growth
Effect of solute and water activity on growth
Effect of oxygen concentration on growth
Nutritional categories of microorganisms
1. Study and plot the growth curve of E. coli by tubidiometric and standard plate count methods.
2. Calculations of generation time and specific growth rate of bacteria from the graph plotted with the
given data
3. Effect of temperature on growth of E. coli
4. Effect of pH on growth of E. coli
5. Demonstration of alcoholic fermentation
6. Demonstration of the thermal death time and decimal reduction time of E. coli.
SUGGESTED READINGS
1. Madigan MT, and Martinko JM (2014). Brock Biology of Microorganisms. 14th edition. Prentice
Hall International Inc.
2. Moat AG and Foster JW. (2002). Microbial Physiology. 4th edition. John Wiley & Sons
3. Reddy SR and Reddy SM. (2005). Microbial Physiology. Scientific Publishers India
4. Gottschalk G. (1986). Bacterial Metabolism. 2nd edition. Springer Verlag
6. Stanier RY, Ingrahm JI, Wheelis ML and Painter PR. (1987). General Microbiology. 5th edition,
McMillan Press.
7. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th edition.
McGraw Hill Higher Education.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
GE-4: INDUSTRIAL AND FOOD MICROBIOLOGY (THEORY)
SEMESTER – IV
TOTAL HOURS: 60 CREDITS: 4
Unit 5 Principles and methods of food preservation and food sanitation No. of Hours: 9
Physical methods - high temperature, low temperature, irradiation, aseptic packaging
Chemical methods - salt, sugar, benzoates, citric acid, ethylene oxide, nitrate and nitrite
Food sanitation and control – HACCP
SUGGESTED READING
1. Crueger W and Crueger A. (2000). Biotechnology: A textbook of Industrial Microbiology. 2nd
Edition. Panima Publishing Company, New Delhi
2. Patel AH. (1996). Industrial Microbiology .1st Edition. MacMillan India Limited Publishing
Company Ltd. New Delhi, India
3. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An introduction.9th Edition. Pearson
Education
4. Willey JM, Sherwood LM AND Woolverton CJ (2013), Prescott, Harley and Klein’s
Microbiology.9th Edition. McGraw Hill Higher education
5. Casida LE. (1991). Industrial Microbiology. 1st edition. Wiley Eastern Limited.
6. Stanbury PF, Whitaker A and Hall SJ. (2006). Principles of Fermentation Technology. 2nd edition,
Elsevier Science Ltd.
7. Adams MR and Moss MO. (1995). Food Microbiology. 4th edition, New Age International (P)
Limited Publishers, New Delhi, India.
8. Banwart JM. (1987). Basic Food Microbiology. 1st edition. CBS Publishers and Distributors,
Delhi, India.
9. Frazier WC and Westhoff DC. (1992). Food Microbiology. 3rd edition. Tata McGraw-Hill
Publishing Company Ltd, New Delhi, India.
10. Jay JM, Loessner MJ and Golden DA. (2005). Modern Food Microbiology. 7th edition, CBS
Publishers and Distributors, Delhi, India.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
GE-5: MICROBES IN ENVIRONMENT (THEORY)
SEMESTER – IV
TOTAL HOURS: 60 CREDITS: 4
1. Analysis of soil - pH, moisture content, water holding capacity, percolation, capillary action.
2. Isolation of microbes (bacteria & fungi) from soil (28ºC & 45ºC ).
3. Isolation of microbes (bacteria & fungi) from rhizosphere and rhizoplane.
4. Assessment of microbiological quality of water.
5. Determination of BOD of waste water sample.
6. Study the presence of microbial activity by detecting (qualitatively) enzymes (dehydrogenase,
amylase, urease) in soil.
7. Isolation of Rhizobium from root nodules.
SUGGESTED READINGS
1. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications. 4th edition.
Benjamin/Cummings Science Publishing, USA
2. Madigan MT, Martinko JM and Parker J. (2014). Brock Biology of Microorganisms. 14th edition.
Pearson/ Benjamin Cummings
3. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2nd edition, Academic
Press
4. Okafor, N (2011). Environmental Microbiology of Aquatic & Waste systems. 1st edition, Springer,
New York
5. Singh A, Kuhad, RC & Ward OP (2009). Advances in Applied Bioremediation. Volume 17,
Springer-Verlag, Berlin Hedeilberg
6. Barton LL & Northup DE (2011). Microbial Ecology. 1st edition, Wiley Blackwell, USA
Campbell RE. (1983). Microbial Ecology. Blackwell Scientific Publication, Oxford, England.
7. Coyne MS. (2001). Soil Microbiology: An Exploratory Approach. Delmar Thomson Learning.
8. Lynch JM & Hobbie JE. (1988). Microorganisms in Action: Concepts & Application in Microbial
Ecology. Blackwell Scientific Publication, U.K.
9. Martin A. (1977). An Introduction to Soil Microbiology. 2nd edition. John Wiley & Sons Inc. New
York & London.
10. Stolp H. (1988). Microbial Ecology: Organisms Habitats Activities. Cambridge University Press,
Cambridge, England.
11. Subba Rao NS. (1999). Soil Microbiology. 4th edition. Oxford & IBH Publishing Co. New Delhi.
12. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th edition.
McGraw Hill Higher Education.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
GE-6: MEDICAL MICROBIOLOGY AND IMMUNOLOGY (THEORY)
SEMESTER – IV
TOTAL HOURS: 60 CREDITS: 4
Unit 1 Normal microflora of the human body and host pathogen interaction
No. of Hours: 8
Normal microflora of the human body: Importance of normal microflora, normal microflora of skin,
throat, gastrointestinal tract, urogenital tract
Host pathogen interaction: Definitions - Infection, Invasion, Pathogen, Pathogenicity, Virulence,
Toxigenicity, Carriers and their types, Opportunistic infections, Nosocomial infections. Transmission
of infection,
Unit 7 Antimicrobial agents: General characteristics and mode of action No. of Hours: 7
Antibacterial agents: Five modes of action with one example each: Inhibitor of nucleic acid synthesis;
Inhibitor of cell wall synthesis; Inhibitor of cell membrane function; Inhibitor of protein synthesis;
Inhibitor of metabolism
Antifungal agents: Mechanism of action of Amphotericin B, Griseofulvin
Antiviral agents: Mechanism of action of Amantadine, Acyclovir, Azidothymidine
SUGGESTED READING
1. Ananthanarayan R. and Paniker C.K.J. (2009) Textbook of Microbiology. 8th edition, University
Press Publication
2. Brooks G.F., Carroll K.C., Butel J.S., Morse S.A. and Mietzner, T.A. (2013) Jawetz, Melnick and
Adelberg’s Medical Microbiology. 26th edition. McGraw Hill Publication
3. Goering R., Dockrell H., Zuckerman M. and Wakelin D. (2007) Mims’ Medical Microbiology. 4th
edition. Elsevier
4. Willey JM, Sherwood LM, and Woolverton CJ. (2013) Prescott, Harley and Klein’s Microbiology.
9th edition. McGraw Hill Higher Education
5. Abbas AK, Lichtman AH, Pillai S. (2007). Cellular and Molecular Immunology. 6th edition
Saunders Publication, Philadelphia.
6. Delves P, Martin S, Burton D, Roitt IM. (2006). Roitt’s Essential Immunology.11th edition Wiley-
Blackwell Scientific Publication, Oxford.
7. Goldsby RA, Kindt TJ, Osborne BA. (2007). Kuby’s Immunology. 6th edition W.H. Freeman and
Company, New York.
8. Richard C and Geiffrey S. (2009). Immunology. 6th edition. Wiley Blackwell Publication.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
GE-7: GENETIC ENGINEERING AND BIOTECHNOLOGY (THEORY)
SEMESTER –VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Brown TA. (2010). Gene Cloning and DNA Analysis. 6th edition. Blackwell Publishing, Oxford,
U.K.
2. Clark DP and Pasternik NJ. (2009). Biotechnology: Applying the Genetic Revolution. Elsevier
Academic Press, USA
3. Primrose SB and Twyman RM. (2006). Principles of Gene Manipulation and Genomics, 7th edition.
Blackwell Publishing, Oxford, U.K.
4. Sambrook J and Russell D. (2001). Molecular Cloning-A Laboratory Manual. 3rd edition. Cold
Spring Harbor Laboratory Press
5. Wiley JM, Sherwood LM and Woolverton CJ. (2013). Prescott, Harley and Klein’s Microbiology.
8th edition, McGraw Hill Higher Education
6. Brown TA. (2007). Genomes-3. Garland Science Publishers
7. Primrose SB and Twyman RM. (2008). Genomics: Applications in human biology. Blackwell
Publishing, Oxford, U.K.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
GE-8: MICROBIAL GENETICS AND MOLECULAR BIOLOGY (THEORY)
SEMESTER – IV
TOTAL HOURS: 60 CREDITS: 4
1. Study of different types of DNA and RNA using micrographs and model / schematic
representations
2. Study of semi-conservative replication of DNA through micrographs / schematic
representations
3. Estimation of salmon sperm / calf thymus DNA using colorimeter (diphenylamine
reagent) or UV spectrophotometer (A260 measurement)
4. Resolution and visualization of DNA by Agarose Gel Electrophoresis.
5. Resolution and visualization of proteins by Polyacrylamide Gel Electrophoresis (SDS-PAGE).
6. Study the effect of chemical (HNO2) and physical (UV) mutagens on bacterial cells
7. Study survival curve of bacteria after exposure to ultraviolet (UV) light
8. Demonstration of Bacterial Transformation and calculation of transformation efficiency.
SUGGESTED READINGS
1. Watson JD, Baker TA, Bell SP, Gann A, Levine M and Losick R (2008) Molecular Biology of the
Gene, 6th edition, Cold Spring Harbour Lab. Press, Pearson Publication
2. Becker WM, Kleinsmith LJ, Hardin J and Bertoni GP (2009) The World of the Cell, 7th edition,
Pearson Benjamin Cummings Publishing, San Francisco
3. De Robertis EDP and De Robertis EMF (2006) Cell and Molecular Biology, 8th edition. Lippincott
Williams and Wilkins, Philadelphia
4. Karp G (2010) Cell and Molecular Biology: Concepts and Experiments, 6th edition, John Wiley &
Sons. Inc.
5. Sambrook J and Russell DW. (2001). Molecular Cloning: A Laboratory Manual. 4th Edition, Cold
Spring Harbour Laboratory press.
6. Krebs J, Goldstein E, Kilpatrick S (2013). Lewin’s Essential Genes, 3rd Ed., Jones and Bartlett
Learning
7. Gardner EJ, Simmons MJ, Snustad DP (2008). Principles of Genetics. 8th Ed. Wiley-India
8. Klug WS, Cummings MR, Spencer, C, Palladino, M (2011). Concepts of Genetics, 10th Ed.,
Benjamin Cummings
9. Maloy SR, Cronan JE and Friefelder D(2004) Microbial Genetics 2nd EDITION., Jones and Barlett
Publishers
10. Russell PJ. (2009). i Genetics- A Molecular Approach. 3rd Ed, Benjamin Cummings
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
SE-1: Microbial Quality Control in Food and Pharmaceutical Industries
SEMESTER – IV
TOTAL HOURS: 30 CREDITS: 2
Unit 4 HACCP for Food Safety and Microbial Standards No. of Hours: 4
Hazard analysis of critical control point (HACCP) - Principles, flow diagrams, limitations
Microbial Standards for Different Foods and Water – BIS standards for common foods and drinking
water
SUGGESTED READING
1. Harrigan WF (1998) Laboratory Methods in Food Microbiology, 3rd ed. Academic Press
2. Garg N, Garg KL and Mukerji KG (2010) Laboratory Manual of Food Microbiology I K
International Publishing House Pvt. Ltd.
3. Jay JM, Loessner MJ, Golden DA (2005) Modern Food Microbiology, 7th edition. Springer
4. Baird RM, Hodges NA and Denyer SP (2005) Handbook of Microbiological Quality control in
Pharmaceutical and Medical Devices, Taylor and Francis Inc.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
SE-2: MICROBIAL DIAGNOSIS IN HEALTH CLINICS
SEMESTER – IV
TOTAL HOURS: 30 CREDITS: 2
SUGGESTED READING
Suggested Readings
Suggested Readings
1. Hui YH, Meunier-Goddik L, Josephsen J, Nip WK, Stanfield PS (2004) Handbook of food and
fermentation technology, CRC Press
2. Holzapfel W (2014) Advances in Fermented Foods and Beverages, Woodhead Publishing.
3. Yadav JS, Grover, S and Batish VK (1993) A comprehensive dairy microbiology, Metropolitan
4. Jay JM, Loessner MJ, Golden DA (2005) Modern Food Microbiology, 7th edition. Springer
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
SE-5: MANAGEMENT OF HUMAN MICROBIAL DISEASES
SEMESTER – IV
TOTAL HOURS: 30 CREDITS: 2
Suggested Readings
1. Ananthanarayan R. and Paniker C.K.J. (2009) Textbook of Microbiology. 8th edition, University
Press Publication
2. Brooks G.F., Carroll K.C., Butel J.S., Morse S.A. and Mietzner, T.A. (2013) Jawetz, Melnick and
Adelberg’s Medical Microbiology. 26th edition. McGraw Hill Publication
3. Goering R., Dockrell H., Zuckerman M. and Wakelin D. (2007) Mims’ Medical Microbiology. 4th
edition. Elsevier
4. Willey JM, Sherwood LM, and Woolverton CJ. (2013) Prescott, Harley and Klein’s Microbiology.
9th edition. McGraw Hill Higher Education
5. Madigan MT, Martinko JM, Dunlap PV and Clark DP. (2014). Brock Biology of Microorganisms.
14th edition. Pearson International Edition
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
SE-5: MICROBIOLOGICAL ANALYSIS OF AIR AND WATER
SEMESTER – III/IV
TOTAL HOURS: 30 CREDITS: 2
Bioaerosols, Air borne microorganisms (bacteria, Viruses, fungi) and their impact on human health
and environment, significance in food and pharma industries and operation theatres, allergens
Suggested Reading
1. da Silva N, Taniwaki MH, Junqueira VC, Silveira N, Nascimento MS, Gomes RAR (2012)
Microbiological Examination Methods of Food and WaterA Laboratory Manual, CRC Press
2. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications. 4th
edition. Benjamin/Cummings Science Publishing, USA
3. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2nd edition,
Academic Press
4. Hurst CJ, Crawford RL, Garland JL, Lipson DA (2007) Manual of Environmental
Microbiology, 3rd edition, ASM press
SCHEME FOR CHOICE BASED CREDIT SYSTEM IN B.Sc.
MICROBIOLOGY HONOURS
Bacteriology
Cell Biology
Molecular Biology
V Industrial DSE-1
Microbiology
Immunology DSE-2
Generic GE-4 2
Elective -
4
Practical
Total: 140