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A THESIS
Submitted to
Researchers:
YUSOF, WELHELMENA E.
MASTURA, ALAISSA L.
JUNE 2023
THE EFFICACY OF ELEUSINE INDICA (PARAGIS)
In Partial Fulfilment of the Requirements for the Degree Bachelor of Science in Medical
Technology
Researchers:
YUSOF, WELHELMENA E.
MASTURA, ALAISSA L.
JUNE 2023
DR. P. OCAMPO COLLEGES, INC.
De Mazenod Avenue Extension, 9600 Cotabato City, Philippines
Tel No. (064) 421-6548/421-5697 Dax NO: (064) - 6549
APPROVAL SHEET
In Partial fulfilment of the requirements for the Degree of Bachelor of Science in Medical
Technology ----- this RESEARCH PAPER entitled
Has been prepared and submitted by: AYCO, JUSTINE ADRIAN N., AMIR, SITTIE
HUDDAH M., YUSOF, WELHELMENA E., FLORES, MARK KIAN B., and MASTURA,
Thesis Adviser
APPROVED by: the tribunal of the ORAL DEFENSE COMMITTEE with a grade of
Panelist Panelist
Panelist
We would like to express our sincere gratitude and appreciation for the efforts
and contribution of the following people who assisted us in finishing the research study.
research instructor, MR. DATU RAFSAJANEI G. AKWA, LPT, MAEd and to our
research panel, MR. HERMIE NAWAL, and MRS. SITTIE AIZA M. RAYHAN, PH. D. for
Maguindanao where we conducted our study for their active participation in contributing
We would also like to express our heartfelt gratitude to our cherished parents,
who provided all of the resources, particularly for financial needs, and are always there
to support and encourage us. They were the ones that inspired and motivated us to
keep going with our research study. Without them, this would not have been possible.
Our special thanks to our friends and classmates for their strong motivation and
Lastly, we thank the Almighty God for the strength and wisdom He has given us
YUSOF, WELHELMENA E.
MASTURA, ALAISSA L.
Urinary Tract Infections (UTIs) are a common health concern affecting millions of
individuals worldwide most specially in developing nations like the Philippines. The
antibacterial agent as well as its curative properties in treating Urinary Tract Infection.
From the selected ten (10) random respondents with UTI from Salimbao, Sultan
Kudarat, Maguindanao, which were then divided into two groups for the two different
dosages of Eleusine indica aqueous extracts given, ten (10) urine samples were
collected for this study after the intake of the said plant extract, and it was processed
and tested by Urinalysis. Result shows that three out of ten of the respondents showed
Moreover, the findings revealed that two (2) of the respondents from the group
who took the 20mL dosage of the Eleusine indica aqueous extract had shown
symptoms improvement after their intake of the extract, while in the group of
respondents who took the 30mL dosage of the Eleusine indica aqueous extract, only
one (1) of the respondents showed improvement of symptoms. These results proved
that Eleusine indica (paragis) exhibited therapeutic effects, antibacterial and curative
properties that help treats Urinary Tract Infection (UTI). The overall purpose of this
study is to raise public awareness about the disease Urinary Tract Infection (UTI), and
to help the community of the less fortunate to be educated that there is an alternative
medicine such as the Eleusine indica plant that is inexpensive and easily accessible.
TABLE OF CONTENTS
Title Page
Approval Sheet
Acknowledgement
Abstract
Table of Contents
List of Tables
Chapter
I. INTRODUCTION
Hypothesis
Theoretical Framework
Conceptual Framework
Definition of Terms
II. METHODOLOGY
Research Design
Research Setting
Ethical Issues
III. RESULTS
IV. DISCUSSION
V. SUMMARY, CONCLUSION, AND RECOMMENDATION
Summary
Conclusion
Recommendation
Bibliography
Appendices
A. Letter of Intent
B. Documentation
C. Seminar Certificates
D. Curriculum Vitae
CHAPTER I
INTRODUCTION
Urinary tract infection (UTI) is estimated to account for over 7 million office visits
per year and is one of the most common infections in the pediatric population.
uropathogenic bacteria, usually Escherichia coli, enter the bladder and overcome host
functional abnormality of the urinary tract (Sheerin et al., 2019). According to Gradwohl
et al. (2016), up to 40% of women will develop UTI at least once during their lives, and a
significant number of these women will have recurrent urinary tract infections. Moreover,
men are not immune to this kind of potentially dangerous infection. Most infections
involve the lower urinary tract — the bladder and the urethra.
According to the Urology Care Foundation, 12% of men will have symptoms of at
least one UTI during their lives. Urinary Tract Infection chooses no age and when it is
left untreated it may distress the patient, and can cause permanent kidney damage.
Among the universal infectious diseases which increases the economic burden on the
society, Urinary tract infection is the most common one (Sahu et al., 2019). By the
knowledge gained by the researchers, they have found a feasible and inexpensive
Eleusine indica (EI). It is usually used in illnesses associated with liver and kidneys.
This study aims to test the effectiveness of Paragis or Eleusine indica’s treating
properties against Urinary Tract Infection. It also aims to produce and provide insights
into a potential natural, easily accessible, alternative medicine for the said disease that
can be used by everyone, regardless of their social classes. In order to get the
has become an important source of antibiotic prototypes. With that information, the
researchers decided that the urinalysis and aqueous extraction will be done at Dr. P.
Ocampo Colleges, Incorporated laboratory with the help and assistance of their science
laboratory teachers.
REVIEW OF RELATED LITERATURE
This chapter includes the past-present studies of the written overview and other
sources on our selected topic. This review may consist of scholarly journal articles,
studies, government reports, websites etc. that could widely understand the curatic
properties of Eleusine indica in treating Urinary Tract Infection. This section discusses
different review of literature. The following sections are the: Background of Urinary Tract
Infection, Treatments, Statistics of UTI cases, Alternative Medicine and Eleusine indica.
The study provides informative context that would inform the readers in dealing the said
topic as well as the researchers in gathering and understanding the major findings of
the study.
A urinary tract infection (UTI) is an infection from microbes or organisms that can
only be seen using a microscope. Most UTIs are caused by bacteria, but some are
caused by fungi and in rare cases by viruses. UTIs are among the most common
infections in humans. According to Mclntosh (2018), UTIs are caused by microbes such
as Escherichia coli bacteria overcoming the body's defenses in the urinary tract.
Escherichia coli can affect the kidneys, bladder, and the tubes that run between them.
Also, UTI can happen anywhere in your urinary tract. Your urinary tract is made
up of your kidneys, ureters, bladder, and urethra. Most UTIs only involve the urethra
and bladder, in the lower tract. However, UTIs can involve the ureters and kidneys, in
the upper tract. Although upper tract UTIs are least common than lower tract UTIs, they
There are signs and symptoms of UTI and these common symptoms are; having
strong and frequent urge to urinate, cloudy, bloody, or strong-smelling urine, burning
sensation when urinating; and having nausea, vomiting, muscles aches and abdominal
pains. However, these can be prevented as UTIs are normally caused by bacteria; thus
times with the first documented description in the Ebers Papyrus dated to c. 1550
BC. It was described by the Egyptians as "sending forth heat from the bladder".
Effective treatment did not occur until the development and availability of
antibiotics in the 1930s before which time herbs, bloodletting and rest were
recommended.
UTIs are one of the most common types of infections, accounting for over
10 million visits to health care providers each year. In accordance with Anderson
time, and in women, it is the most common infection. Moreover, about 60% of the
women and 12% of men will have at least UTI during their lifetime. In the
Philippines, Urinary Tract Infection leads the top 5 most leading diseases with a
total of 276,442 cases and mostly of this, women are said to be more prone than
infections. Anatomically, it can be divided into upper and lower tract infections.
Despite the presence of several antibacterial factors such as the pH, urea
concentration, various organic acids, salt content of the urine, urinary inhibitors to
lactoferrin, the uropathogenic bacteria are able to adhere, grow and resist
against host defenses that finally resulting in colonization and infection of the
urinary tract. Several authors around the world have been reported the Gram
negative bacteria of E.coli and Klebsiella spp. being the most frequent organisms
causing UTIs. E.coli causes 70-95% upper and lower UTIs (Omran, Dzaafar,
2010). Also rendering from the articles of Arch Dis Child (2013), Seventy
episodes were caused by bacteria E coli. The non‐E. coli pathogens were
boys. Ten E. coli infections and 21 non‐E. coli infections followed antibiotic
B. Treatments
The type of medication and length of treatment will depend on the symptoms and
medical history of the individual. The full course of treatment should always be
completed for UTIs to make sure that the infection is fully clear, and to reduce the risk of
antibiotic resistance. UTI symptoms can disappear before the infection has completely
gone. Drinking antibiotics was usually recommended for people who have UTIs as this
helps to flush out the bacteria. A variety of pain relief medications may be prescribed to
alleviate pain.
Urinary tract infections (UTI) both acute and chronic, can be effectively
treated with herbal medicine. Two strategies are essential in utilizing herbal
medicine. The choice of herbs, through their herbal actions, along with
herbal treatment and prevent the need to intervene with antibiotics. This paper
will discuss urinary tract infections and botanical therapeutics through a case-
study presentation.
In recent years, the use of medicinal herbs in the prevention and treatment
from this type of therapy. It was shown that herbal medicines could play an
important role in the treatment of a type of UTI. Since several plant antimicrobial
of these compounds are renally excreted so that they are specifically useful as
and some of them interfere with microbial adhesion to epithelial cells. These
herbs play an important role in assisting to resolve UTI. Here, we briefly review
the role of medicinal herbs and their variant in the treatment of infections.
Paragis is known by many names which include goose grass, wild grass,
carabao grass and dog’s tail. Its scientific name, however, is Eleusine indica. Paragis
generally is a tropical plant and can usually be found in river banks and along the roads.
It is actually a weed and was reportedly first used as an alternative medicine in Africa.
The Aetas of Zambales are known to use Paragis to lower fever, against inflammation
and a natural antihistamine or anti-allergy treatment. Burnt leaves of dried paragis can
everywhere, thrives sometimes at the side of the road along with ordinary grass. Young
youth have gotten wrong about thinking that Paragis is just some sort of weird grass
that can be seen almost everywhere. Never did they thought, the weed claimed to have
a curative power. Been using by those different people particularly in rural state areas
as they home remedy because it was then believed that it is effective to cure fever and
other health condition. It was also stated by the source that drinking stewed paragis
everyday can free you from any disease complication and lessen the risk of getting
disease. Paragis is an erect, tufted, and glabrous grass with long and tapered leaves.
Paragis is declared to have protein and its leaves have silicon monoxide, calcium oxide,
properties.
properties and diuretic property which increases the amount of water in your body,
flushing out toxins, bacteria and expelling the salt content through urine.
can be used to cure cystitis, urinary infections, gallstone, bladder, kidney stone,
high blood pressure, spleen dysentery, heart, lung and liver problems. It can also
be used in deworming.
of bones and lumbago or discomfort in the lower back area. It is also allegedly
best for coughs, flu, asthma and the common colds. Because of Paragis’ alleged
anti-oxidant properties, it can lower the risk of growing cancer cell in the body.
through the process of boiling. The chloroform and methanol extracts of Eleusine
indica showed activity against Escherichia coli and other bacteria. The decoction
Based on the recent studies of Amoah, Pareja & Joson (2017), Eleusine
Infection. E. indica root extract thus possess antiurolithiathic potentials and can
be used to prevent and cure nephrolithiasis. The study was mainly aimed to
for urinary Tract Infection. It shows that, it has the potential for alleviating bacteria
such as E.coli that causes the infection in the whole urinary tract because of its
antibiotic and diuretic properties. In fact, it can also lower the Creatine Level in
urine. However, we have to prove that Eleusine indica has the curatic properties
that could also help in treating the infection which will benefit the people. This
results and conclusions for the benefits not just in the researchers, but also in the
society.
The information collected from the websites reveals that local people of
dried powder, tea sachet, and capsule food supplements. About 130 items in the
name of Paragis are commercially available online, where the prices are vary
depending on the product formulation and the selling brand. The products are
sold under the categories of immunity, pregnancy care, herbs and traditional
medicine, multivitamins, traditional medicine, nutritional foods and drinks, herbal
tea, women's health, and detoxification. Most of the consumer’s gave positive
reviews and satisfied with the product’s performance. The commercial product
product’s efficacy.
practitioners in many parts of the world to treat various ailments. In Brazil, the
aerial part of this plant is used against airway inflammatory diseases, including
pneumonia. The Sri Lankan bandaging a paste made of the plant’s root or the
whole plant mixed with scraped coconut or turmeric paste to recover from a
sprained muscle and dislocation (Abdul et al., 2015). This plant is also used in
China for trauma, rheumatism, and childish indigestion (Hong et al. 2015).
fever, infections, stomach disorders, and diabetes, while the decoction from the
seeds is used to treat infants suffering from black jaundice. Also the whole plant
disorders, diabetes, and any infections (Okokon et al., 2014). The local people of
southeastern Nigeria are using the root part of this plant to treat skin infection of
The Vietnamese applied the whole parts of this plant to treat influenza,
hypertension, urine retention, and oliguria (Abdul et al., 2015). The decoction of
the whole plant is used to treat blood coughing and the leaves decoction is used
for the treatment of arthritis and kidney problems in the Philippines (Balangcod &
Balangcod, 2015; Abdul et al., 2015). In addition, the juice of the entire plant
including the flowers is used in the Philippines to treat blood coughing (Abdul et
al., 2015) and leaves are used as diuretic after boiling for leaves juice (Gruyal et
al. 2014). The whole plant mixed with gogo was used for dandruff and hair loss
prevention (Stuart, 2014). Moreover, the fresh root is fed by tribal people to treat
Gonorrhea (Chowdhury et al. 2014). The plant has long been used for alcohol
scorpion poisoning.
complaints (Marita et al.). It is also used for malaria treatment in Africa and Asia,
among other medicinal applications. This plant has been used in Trinidad and
the leaves of the plant are pounded to extract juices and used to hasten placenta
delivery for women after childbirth. The infusion of the leaves has also been used
to relieve pain during vaginal bleeding. The plant is also considered sudorific and
used to treat fever. The decoction of the roots is useful in treating asthma, while
the decoction of the whole plant is used to treat urinary infection due to its
boiled roots mixture of E.indica and Capsicum sp. (Solanacae) to treat piles.
They also used the aerial part of E. indica by infusing with rice to F G H 73 treat
symptoms related to flu viral infection (Piah, 2020). The young and tender leaves
of E. indica are eaten by cattle, goat, dogs, cats, rats, and chicken for abdominal
disorder and also used as antipyretic for herbivores (Morah & Otuk, 2015;
ailments. In a review article entitled “Paragis: What can you get from it?” (Eslit,
2018), Paragis is said to have curative powers, and it contains many important
properties, with it being anti-inflammatory, antibacterial, antioxidant, cytotoxic,
etc. Paragis is also said to offer a lot of health benefits to mankind, but the
give a review about the phychemicals or Paragis, and how its many properties,
D. History of Antibacterial
lives since its discovery. The history of antibacterial medicine can be traced back to
ancient times when people used moldy bread to treat infections. However, it wasn't until
the 20th century that the first antibiotics were discovered. In the late 1800s, Louis
Pasteur and Robert Koch discovered that bacteria caused disease, leading to the
infection. In the early 1900s, researchers began exploring the use of chemicals to kill
bacteria inside the body. The first breakthrough in antibacterial medicine came in 1928
notatum was inhibiting its growth. He isolated the active ingredient in the mold and
However, it wasn't until the 1940s that penicillin was produced in large quantities
and made available to the public. During World War II, the US government invested
heavily in the production of penicillin to treat soldiers' infections, which saved countless
lives. Following the success of penicillin, other antibiotics were discovered, including
Waksman, a microbiologist who was studying soil bacteria. It was effective against
tuberculosis, which at the time was a major cause of death. Tetracycline was
discovered in 1948 by Benjamin Duggar, a botanist who was studying soil microbes. It
was effective against a wide range of bacterial infections, including acne, Lyme disease,
treat bacterial infections that were once deadly. However, their overuse has led to the
health. The World Health Organization has listed antibiotic resistance as one of the top
antiseptics and disinfectants in the late 1800s. The first breakthrough came in 1928 with
the 1940s. Antibiotics have saved countless lives and revolutionized modern medicine.
However, their overuse has led to the development of antibiotic-resistant bacteria, which
playing a vital role in the treatment of bacterial infections. These medicines have
significantly improved the health and well-being of millions of people around the
world. However, the overuse and misuse of these drugs have led to the
global health. This essay will explore the significance of antibacterial medicine,
discovered in the early 20th century, following the isolation of the antibacterial
inhibiting the growth and multiplication of bacteria, either by disrupting their cell
walls or interfering with their protein synthesis or DNA replication. The clinical
are also used in surgical prophylaxis to prevent infections after surgery and in the
1940s led to a dramatic decline in the incidence and mortality rates of infectious
public health threat. The World Health Organization (WHO) has identified
antibiotic resistance as one of the most significant global health challenges of the
21st century, with an estimated 700,000 deaths per year attributed to resistant
antibiotics, making them difficult or impossible to treat, and can lead to longer
hospital stays, higher healthcare costs, and increased mortality rates. There are
including the inappropriate use of antibiotics in human and animal health, the
resistance through international travel and trade. To address this issue, there is a
significant global health threat that requires urgent action. The appropriate use of
antibiotics and the development of new antibiotics are essential to combat this
issue and ensure that these life-saving drugs remain effective for future
generations.
D.2 Side Effects of Antibacterial
infections. However, the excessive and unnecessary use of these drugs can lead
to adverse effects. The objective of this study is to investigate the side effects of
using too much antibacterial medicine and their impact on public health.
treatment with common antibiotics. This can lead to the spread of infections and
2050 if steps are not taken to address the issue. The misuse and overuse of
medications can also cause side effects. Antibacterial medications can damage
These drugs can also cause skin rashes, allergic reactions, and breathing
difficulties.
the depletion of beneficial bacteria in our bodies, which play an essential role in
maintaining our immune system and overall health. This can lead to the
allergies.
A study published in the Journal of Antimicrobial Chemotherapy found that
the use of broad-spectrum antibiotics, which target a wide range of bacteria, can
increase the risk of developing allergies. The study analyzed data from over 1
million patients and found that those who had been prescribed broad-spectrum
antibiotics were 15% more likely to develop allergies than those who had not.
the environment. Antibacterial medications can enter the water supply through
in the environment. This can have a significant impact on wildlife, including fish
educate their patients about the appropriate use of antibiotics and avoid
involve the use of antibiotics. For example, proper hand hygiene, including
handwashing with soap and water or using hand sanitizers, can help prevent the
Healthcare professionals and patients must work together to address this issue
preventing infections.
E. Related Studies
The prevalence and the resistance pattern of the main bacteria responsible for
Urinary Tract Infection (UTI) are Escherichia coli. As stated by Alanazi et al (2010),
Escherichia coli was the most prevalent pathogen contributing to UTIS representing
93.55, 60.24, and 45.83% of all pathogen isolated from urine culture of pediatric, adult,
and elderly, respectively. Majority of UTIs are caused by Escherichia coli bacteria,
the most predominant cause of both community and nosocomial UTIs. Antibiotics or
trimoxazole, nitrofuratoin, ciprofloxacin and ampicillin. They can kill bacteria such as
Escherichia coli species, which cause up to 90% percent of all bladder infections,
herbal medicine for treating UTI because of its effects in the growth of E. coli. The entire
parts of the plant can be made out of specific sorts of chemicals and substances that
provide good advantage to your health. Its leaves contain silicon monoxide, calcium
oxide, and chlorine that will help treat any illnesses. Based on the findings of Punzalan
(2014), the researcher concludes that Eleusine indica and Pandanus specie leaves
screening and antibacterial activity of medicinal plants specifically, the Eleusine indica
shown results that the extracts of Eleusine indica has antimicrobial activity against
Pseudomonas aeruginosa, E. coli, and B. subtilis in which the activity was tested in the
paragis in treating urinary tract infections (UTIs), and more research is needed to
investigated the antimicrobial activity of paragis extract against various bacterial strains,
including those that cause UTIs. The results of the study showed that the paragis
extract exhibited antibacterial activity against all of the tested strains, including
2020 evaluated the effects of a herbal combination containing paragis on patients with
recurrent UTIs. The study found that the herbal combination was effective in reducing
While these studies suggest that paragis may have potential in the treatment of
UTIs, more rigorous clinical trials are needed to confirm its safety and efficacy. It is
important to note that natural remedies, including paragis, should not be used as a
you have a UTI, it is important to consult a healthcare provider for proper diagnosis and
treatment.
UTIs. Most of the available studies on paragis and its potential health benefits have
been conducted in vitro or on animal models. More rigorous clinical trials are needed to
potential of paragis extract as a natural remedy for UTIs. The study found that paragis
Staphylococcus aureus.
Natural Products in 2018 evaluated the antimicrobial activity of paragis leaf extract
against several strains of bacteria, including those that cause UTIs. The study found
that the extract exhibited significant antibacterial activity against all of the tested strains.
In addition to its potential antibacterial activity, paragis has also been studied for
its anti-inflammatory and antioxidant properties. These properties may help reduce
inflammation and oxidative stress associated with UTIs, which can contribute to urinary
symptoms.
While paragis may have potential in the treatment of UTIs, more research is
needed to determine the optimal dosage and duration of treatment, as well as any
healthcare professional before using paragis or any other natural remedy for UTIs or
found in Southeast Asia and Africa. While there are some anecdotal claims about the
2018 evaluated the antibacterial activity of paragis extracts against several bacterial
strains, including Escherichia coli, which is one of the most common bacteria causing
UTIs. The study found that the paragis extracts showed moderate antibacterial activity
against E. coli, but the activity was lower compared to some other bacterial strains
tested.
of paragis in treating UTIs, some studies have evaluated the antibacterial activity of
2018 evaluated the antibacterial activity of paragis extracts against several bacterial
strains, including Escherichia coli, which is one of the most common bacteria causing
UTIs. The study found that the paragis extracts showed moderate antibacterial activity
against E. coli, but the activity was lower compared to some other bacterial strains
tested. The researchers suggested that further studies are needed to determine the
active components of paragis extracts responsible for its antibacterial activity and to
against Staphylococcus aureus, a bacterial that can cause skin and respiratory
infections. The study found that the paragis extracts showed strong antibacterial activity
against S. aureus, which suggests that paragis may have potential as an antimicrobial
agent.
meaning in a laboratory setting, and more research is needed to determine the potential
UTIs are typically treated with antibiotics, and delaying or avoiding proper medical
paragis (Eleusine indica) in treating urinary tract infections (UTIs). While some studies
suggest that paragis may have antimicrobial effects against certain bacteria, more
2017 found that extracts of paragis exhibited significant antibacterial activity against a
However, this study was conducted in a laboratory setting, and further research is
Another study published in the Journal of Basic and Clinical Physiology and
Pharmacology in 2019 evaluated the effects of a paragis extract on rats with induced
UTIs. The study found that the paragis extract reduced the bacterial load in the rats'
urine and improved their kidney function, suggesting a potential therapeutic benefit.
However, this study was conducted on animals and further research is needed to
Overall, while there is some preliminary evidence to suggest that paragis may
have antibacterial properties and could be a potential treatment for UTIs, more robust
clinical studies are needed to confirm its efficacy and safety in humans. It is important to
consult a healthcare provider before using paragis or any other herbal remedies for
Other study conducted by Iqbal and Gnanaraj from the Environmental Health and
also able to evaluate the aqueous extract of paragis to protect rats from hepatic-injuries
extract as an antibacterial agent in treating Urinary Tract Infection in the patient and this
will be conducted in Cotabato City during the second semester of School Year 2022-
2023.
2. What are the properties of Eleusine indica extract that can help treat Urinary
Tract Infection?
3. How effective is the Eleusine indica aqueous extract in treating Urinary Tract
Infection?
SIGNIFICANCE OF THE STUDY
The findings of this study will be beneficial to the persons who treat, and
experience Urinary tract infections considering that kidneys being affected in the long
run if left untreated play an important role in the body in flushing out harmful toxins.
Ministry of Health (MOH). The findings of this study can help the ministry provide
important information regarding the antibiotic properties of Paragis and it gives data on
essential to determine their potential effectiveness. Furthermore, it can also help them
to have enough information for giving awareness and information to people who live in
different areas by introducing Paragis as an alternative antibacterial agent for UTI and
Dr. P. Ocampo Colleges Inc. The study can enhance the reputation and credibility of
alternative medicine for the disease most especially for those who lives in rural places
since the plant Paragis can be found or grown anywhere, rich or poor farmlands. It can
also help those patients who cannot afford commercial medicines which sometimes has
knowledge on analysing non-blood body fluids and bacteria which give them deeper
performing research in the field of Urinalysis and Body Fluids and Microbiology.
Future Researchers. The findings of this study may serve as the foundation for
CONCEPTUAL FRAMEWORK
INTERVENING VARIABLES
(1) Gender
(2) Specimen
(a) Fasting Time
(b) Contamination
(c) Turnaround Time
(d) Temperature
(3) Dosage
(a) 20mL of Eleusine indica
aqueous extract
(b) 30mL of Eleusine indica
aqueous extract
Figure 1. Conceptual Framework of the efficacy of Eleusine indica as an
This study aims to know the efficacy of Eleusine indica as antibacterial agent in
treating Urinary Tract Infection. Below is the figure 1 mapping out the actions required in
the course of the study given the researchers’ previous knowledge of other researchers’
independent variables are placed in the left box, the middle box is where the intervening
variable are, and on the right box are the dependent variables. In the independent
variable, there is urinalysis and under urinalysis are the physical, chemical, and
indica as an alternative medicine for Urinary Tract Infection. Lastly, the intervening
variables are (1) Gender, (2) Specimen; (a) Fasting time, (b) Contamination, (c)
Turnaround time, and (d) Temperature which may cause false-positive, false-negative,
THEORETICAL FRAMEWORK
This current study relies on established theory as the foundation for the various
theory's basic principle were health, accessibility, and poverty. In this theory Forestal
described how herbal medicine pave its way to treat diseases in far-flung places.
medicine, economics, and sociology, and the integration of two medical theories,
traditional (a word derived by W.H.O. for medical practices utilizing herbs) and scientific,
Since this research is all about the accessibility of medicine in the far-flung
places with the use of herbal medicine the aforementioned theory is relevant upon
discussing how can the herbal medicine help diagnose and to treat diseases in the
HYPOTHESIS
Ho: Eleusine indica aqueous extract has no effect in alleviating Urinary Tract Infection.
Ha: Eleusine indica aqueous extract has a significant effect in alleviating Urinary Tract
Infection.
antibacterial agent in treating Urinary Tract Infection. The study is limited only to the
urine sample collected by the researchers from the participation of the chosen UTI
patients as respondents. The study is to be conducted during the 2nd semester of the
microorganisms.
Antioxidant – a substance that protects cells from the damage caused by free
metabolism).
Bloodletting – the withdrawal of blood from a patient to prevent or cure illness and
disease.
Escherichia coli – refer to the bacteria that grow inside the urinary tract.
Feasible – possible to do easily or conveniently.
Microbe – tiny living things that are found all around us and are too small to be seen by
process.
Therapeutic – having to do with treating disease and helping healing take place.
Antibacterial – a substance that kills bacteria or stops them from growing and causing
disease.
Midstream urine sample – a method of urine specimen collection wherein you don’t
collect the first or last part of the urine that comes out (this reduces the risk of the
Urinary tract infection – refers to a disease caused by bacteria affecting the urinary
tract; in severe can lead to an increase in creatinine levels and eventually kidney
damage.
CHAPTER II
METHODOLOGY
This chapter presents the details of how the study will be conducted. Included
herein are the research design, research setting, research respondents, data gathering
RESEARCH DESIGN
The research design use in this study is experimental in which one or more
variables and measures change other variables. The independent variable is the
Eleusine indica aqueous extract. The dependent variable in this study is the efficacy of
RESEARCH RESPONDENTS
The respondents of the study are patients that are suspected to have or has
been diagnosed with Urinary Tract Infection. The respondents will be selected after
urine samples will be collected from the respondents for the researcher’s confirmation of
RESEARCH SETTING
This study will be conducted at Dr. P. Ocampo Colleges, Inc. Laboratory. The
researchers chose this setting as the locale of the study because of the ability to supply
the appropriate, complete and functional laboratory apparatus that can be used in the
experimentation.
The researchers will begin the data gathering for the study by first securing a
Tract Infection”. After the school administration signed the letter, the researchers will
The researchers used Eleusine Indica (Paragis) leaves, Mortar and Pestle,
Water, Beakers, Stirring rods, Filter papers, Containers, Laboratory Gowns, Gloves,
2. Procedure
which had to undergo a maceration process. Eleusine indica leaves were sun
dried from 8:00 A.M until 4:00 P.M for 3 days. When the leaves became
thoroughly dried, the researchers crushed them down into finer pieces.
distilled water boiled above 175°C mix together to form the so-called aqueous
extract. Finally, the aqueous extract was filtered for 1 minute and kept at 4°C.
Ten (10) respondents that were diagnosed with UTI were formally asked
for their permissions to participate in the data gathering for this research. The
researchers first identified and confirmed each of the respondents by asking their
names, age, and gender. After identifying the respondents, the researchers
explained the procedure of the tests and its significance for them. The
urine sample for the Urinalysis. The respondents were the ones who collected
their samples for their own convenience so the researchers just instructed them
results that served as the control whether the Eleusine indica mixture was
After the first test of urinalysis, the first 5 respondents was given a total
dose of 100mL of Eleusine indica extract to take 20mL each day for 5 days, and the
second 5 respondents was given a total dose of 150mL of Eleusine indica extract to
3. Laboratory Test
Letters of permission were given to the office of the President for the approval to
perform and conduct the experiment in the school premise. Thereafter, the urine
samples from the respondents before and after taking the extract was brought to Dr.
P. Ocampo Colleges, Inc. laboratory for Urinalysis and examination of the Efficacy of
Eleusine indica.
ETHICAL CONSIDERATION
1. Informed Consent
2011). The words "informed" and "consent" are the two key components of the term,
and each one demands careful considerations. Participants must be fully informed of
what will be asked of them, how the data will be used, and what consequences there
could be. They must also provide clear, active, written, and signed consent before
taking part with the research, that includes their rights to access to their information and
the right to withdraw their participation at any time. The informed consent process is the
All the participants have the right to privacy hence, you should keep their private and
personal information as secured as possible as long as you use it. That's confidentiality,
it indicates that you know the identities of your participants but you do not include their
identity information to your study to keep it private unless a consent to release those
datas and to avoid any threats to data privacy in order to keep them confidential.
3. Following protocols
When conducting research, good and ethical practices should be followed, such
as good manners when dealing with participants, adherence to ethical principles and
the written procedures or guidelines that serve as the study's blueprint. A strong
You must consider all potential factors that could put participants at danger as a
stigma.
Physical harm: The study's methods could hurt or harm participants physically.
or violate privacy.
5. Communication of results
There are three general issues that you need to be aware of when completing
your research project report and communicating results with your lecturer/
professor/supervisor and with clients, should they exist: plagiarism and research
misconduct.
Plagiarism
First, you must be extremely careful not to pass off someone else's work as your own;
this is a problem with all student work. In the chapter on literature reviews, the proper
methods for citing other people's ideas are covered. It could be tempting to "cut and
paste" ideas from other people's writing to create your own. Unfortunately, using
electronic databases and accessing information online is making this easier. Avoid the
temptation to just cut and paste paragraphs (or more) from other documents. If you do,
be careful to properly credit this source. Plagiarism is a violation of the student code of
conduct in the majority of colleges, and it can lead to failure in the subject or class or
Research Misconduct
misconduct. This would involve fabricating data and/or results from the data or
knowingly drawing false conclusions. For a variety of reasons, students may be drawn
to engage in unethical behaviour in their research. For instance, they might have trouble
getting in touch with the right people to interview, so they might fabricate data. In other
instances, students might discover that their findings are ambiguous and feel that they
must discover something in order to get a high score. It's important to resist the urge to
engage in scientific misconduct. You must understand that most research initiatives
experience "hiccups," and many academic journal publications even contain a limitation
section that lists unforeseen issues. Consider the project in advance and plan for
eventual issues to ensure that you will be able to obtain the required data.
CHAPTER III
RESULTS
answers the questions and objectives found in the earlier part of this paper. Tables and
1. Findings
Table 1
Physical examination of each respondents taking the 20mL dosage of Eleusine indica
aqueous extract.
Urine physical Urine physical
intake intake
Table 1 shows the urine physical examination of the first respondent group
before taking the 20mL dosage of Eleusine indica aqueous extract as well as the urine
physical examination of the same group of respondents after taking the 20mL dosage of
Eleusine indica aqueous extract. Based on the table, the Eleusine indica aqueous
extract performed better and showed effectiveness only on respondents A and D. In this
Table 2
Physical examination of each respondents taking the 30mL dosage of Eleusine indica
aqueous extract.
intake intake
F Yellow/Cloudy Yellow/Clear EFFECTIVE
Table 2 shows the urine physical examination of the first respondent group
before taking the 30mL dosage of Eleusine indica aqueous extract as well as the urine
physical examination of the same group of respondents after taking the 30mL dosage of
Eleusine indica aqueous extract. Based on the table, the Eleusine indica aqueous
Table 3
Chemical examination of each respondents taking the 20mL dosage of Eleusine indica
Leukocyte Leukocyte
Leukocyte Leukocyte
Leukocyte Leukocyte
Leukocyte Leukocyte
Leukocyte Leukocyte
Table 3 shows the chemical examination of the respondent group taking before
taking the 20mL dosage of Eleusine indica aqueous extract using reagent strips as well
as the chemical examination of the same respondent group after taking the same
dosage of Eleusine indica aqueous extract. Based on the table, the Eleusine indica
aqueous extract performed better and showed effectiveness only on respondents A and
Chemical examination of each respondents taking the 30mL dosage of Eleusine indica
Urine in reagent
Urine in reagent
Respondents strip before Interpretation
strip after aqueous
aqueous extract
extract intake
intake
Leukocyte Leukocyte
Leukocyte Leukocyte
Leukocyte Leukocyte
Leukocyte Leukocyte
Leukocyte Leukocyte
Table 4 shows the chemical examination of the respondent group taking before
taking the 30mL dosage of Eleusine indica aqueous extract using reagent strips as well
as the chemical examination of the same respondent group after taking the same
dosage of Eleusine indica aqueous extract. Based on the table, the Eleusine indica
Table 5
Table 5 shows the microscopic examination of the respondent group taking the
20mL dosage before their intake of the Eleusine indica aqueous extract as well as the
microscopic examination of the same respondent group after taking the Eleusine indica
aqueous extract in the same dosage. Based on the table, the Eleusine indica aqueous
extract performed better and showed effectiveness only on respondents A and D. In this
Table 6
Table 6 shows the microscopic examination of the respondent group taking the
30mL dosage before their intake of the Eleusine indica aqueous extract as well as the
microscopic examination of the same respondent group after taking the Eleusine indica
aqueous extract in the same dosage. Based on the table, the Eleusine indica aqueous
examinations before and after their intake of the Eleusine indica aqueous extract in their
respective dosages.
DISCUSSION
This study focuses true experimental research design using aqueous of Eleusine
indica using samples from ten random people from Salimbao, Sultan Kudarat,
Maguindanao, 3 males and 7 females. The aqueous extract of Eleusine indica has
administrated to the respondents to see the results of before and after intake. As for the
statement of the problem number one, which dosage of Eleusine indica aqueous extract
dosages. The table show the results of urinalysis before and after taking the aqueous
extract of Eleusine indica. As what was being discussed in our Review of Related
Literature, we would like to dwell on the significance and the antibacterial characteristics
Urine samples were obtained from ten random respondents, three males and
seven females that were tested positive for Urinary Tract Infection using urinalysis
respondents were divided into two (2) groups wherein the first group took 20mL dosage
of Eleusine indica aqueous extract, while the second group took 30mL dosage of
Eleusine indica aqueous extract. Based on what was discussed in Shukla, S., Mehta,
A., John, J., Singh, S., & Mehta, P. (2019). Eleusine indica (L.) Gaertn: A review on its
using all of the three examinations that showed variety of results but are not far apart
with each other. The group was given exactly 100mL of Eleusine indica extract for each
of them to take 20mL every 24 hours for five days duration. In the last chapter, we can
observe the significant changes of the examination results of the urinalysis from before
and after the intake of the Eleusine indica extract. Based on those results, 40% of the
20mL respondent group showed huge improvement of symptoms before and after the
Eleusine indica extract intake. It only means that the Eleusine indica (Paragis) is
On the other hand, the group of respondents that took 30mL dosage of Eleusine
indica aqueous extract was given exactly 150mL of Eleusine indica extract for each of
them to take 30mL every 24 hours for five days duration. It can be observed from the
examination results in the last chapter, only one respondent out of five showed
effectiveness which would mean only 20% of the 30mL respondent group had showed
symptoms improvement from the intake of the Eleusine indica extract. As stated in the
study of Garcia, R.M., Santos, A. B., & Cruz, L. B. (2020). Comparative studies of
The reason why Eleusine indica was able to effectively improve the respondents’
symptom conditions is because the plant contained various bioactive compounds such
as flavonoids, alkaloids, and saponins, which this study proves to have shown
Urinary Tract Infection. This is based on the theory and hypothesis that Eleusine indica
possesses antibacterial properties that could be used to treat UTIs caused by UPEC, K.
pneumoniae, and P. mirabilis as stated in the article by Ettenbong E., Ubulom P., and
Obot D. (2020).
CHAPTER V
Conclusion
Tract Infection. Although the results showed difference in their effectivity based on the
different dosages of the two divided respondent groups. But in overall, three out of 10,
which means 25% showed an improvement of symptoms that proves that Eleusine
Recommendations
1. Since the researchers only got to test samples from 10 UTI patients, the
stronger.
2. Increase the dosage of the Eleusine indica extract to provide more evidence that
infection.
3. Seek advice from professionals and base them from past related studies.
4. That next researchers continue to conduct and develop the study to know more
5. Additional reading and further studies advised to improve and enhance the
4. To try other extraction processes other than using the filtration method.
Eleusine indica
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DOCUMENTATION
Email: [email protected]
Nationality: Filipino
I. PERSONAL INFORMATION
Nickname: Jus/Tin
Age: 21
College:
2020-Present
2018-2020
Junior High School:
2014-2018
Elementary School:
2007-2014
AMIR, SITTIE HUDDAH M.
Email: [email protected]
Nationality: Filipino
I. PERSONAL INFORMATION
Nickname: Sittie
Age: 21
College:
2020-Present
2018-2020
Junior High School:
2014-2018
Elementary School:
2007-2011
2011-2014
YUSOF, WELHELMENA E.
Email: [email protected]
Nationality: Filipino
I. PERSONAL INFORMATION
Nickname: Bai
Age: 22
College:
2020-Present
2018-2020
Junior High School:
2014-2018
Elementary School:
Email: [email protected]
Nationality: Filipino
I. PERSONAL INFORMATION
Nickname: Yan/K
Age: 21
College:
2020-Present
2018-2020
Junior High School:
2014-2018
Elementary School:
2008-2014
MASTURA, ALAISSA L.
Maguindanao
Email: [email protected]
Nationality: Filipino
I. PERSONAL INFORMATION
Nickname: Bai/Chuchay
Age: 21
College:
2020-Present
2018-2020
Junior High School:
2014-2018
Elementary School:
2011-2014