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01/17/2024 PhysioEx Exercise 6 Activity 5

PhysioEx Lab Report


Exercise 6: Cardiovascular Physiology
Activity 5: Examining the Effects of Various Ions on Heart Rate
Name: Roland Calipayan Jr.
Date: 17 January 2024
Session ID: session-6b88988f-12e6-dc9d-648b-3fb1f6e545y8

Pre-lab Quiz Results


You scored 100% by answering 4 out of 4 questions correctly.

1 Which organelle in the cardiac muscle cell stores calcium?

You correctly answered: sarcoplasmic reticulum.

2 Verapamil is a calcium-channel blocker. Its effects could be described as

You correctly answered: negative chronotropic and negative inotropic.

3 When the cardiac muscle cell is at rest, where is most of the potassium found? You

correctly answered: in the cytosol.

4 Resting cardiac muscle cells are most permeable to

You correctly answered: potassium.

Experiment Results
Predict Questions

1 Predict Question 1: Because calcium-channel blockers are negative chronotropic and negative
inotropic, what effect do you think increasing the concentration of calcium will have on heart rate?

Your answer: positive chronotropic, positive inotropic.

2 Predict Question 2: Excess potassium outside of the cardiac cell decreases the resting potential
of the plasma membrane, thus decreasing the force of contraction. What effect (if any) do you
think it will initially have on heart rate?

Your answer: decrease heart rate.

Stop & Think Question


1 Where in the cardiac muscle cell is most of the sodium normally found?

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01/17/2024 PhysioEx Exercise 6 Activity 5

You correctly answered: outside of the cell.

Experiment Data

Solution Heart Rate (beats/min)


---- 62
Calcium 72
Sodium ~34
Potassium ~28

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01/17/2024 PhysioEx Exercise 6 Activity 5

Post-lab Quiz Results


You scored 100% by answering 4 out of 4 questions correctly.

1 The addition of most of the ions resulted in

You correctly answered: an erratic, decreased heart rate.

2 The effect of potassium on the heart is

You correctly answered: negative chronotropic, negative inotropic.

3 The ion that had the most pronounced effect on heart rate was You

correctly answered: potassium.

4 Ectopic pacemakers can be caused by excessive leakage of potassium into cardiac cells,
resulting in pacemakers appearing in abnormal locations in the heart muscle. This hyperkalemia
(excess potassium) decreases the resting potential of the cardiac muscle cell. What effect do you
think this would have on the force of contraction?

You correctly answered: decrease, negative inotropic.

Review Sheet Results


1 Describe the effect that increasing the calcium ions had on the heart in this activity. How well did
the results compare with your prediction?

Your answer:

The blood is pumped more forcefully and the heart beats more quickly as expected due to an
increase in heart rate and contraction pressure.

2 Describe the effect that increasing the potassium ions initially had on the heart in this
activity. Relate this to the resting membrane potential of the cardiac muscle cell. How well i
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01/17/2024 PhysioEx Exercise 6 Activity 5

did the results compare with your prediction?

Your answer:

As expected, high potassium ions lower the contraction pressure and resting potential.

3 Describe how calcium channel blockers are used to treat patients and why?

Your answer:

They obstruct the flow of calcium through the channels in every action potential path. Reduced
heart rate, peripheral resistance, and contraction pressure are all brought on by decreased
calcium.

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