Plant-Based Fish Analogs-A Review
Plant-Based Fish Analogs-A Review
Plant-Based Fish Analogs-A Review
sciences
Review
Plant-Based Fish Analogs—A Review
Malgorzata Nowacka 1, * , Magdalena Trusinska 1 , Paulina Chraniuk 2 , Jagoda Piatkowska 1 , Anna Pakulska 1 ,
Karolina Wisniewska 2 , Anna Wierzbicka 1 , Katarzyna Rybak 1 and Katarzyna Pobiega 2
1 Department of Food Engineering and Process Management, Institute of Food Sciences, Warsaw University of
Life Sciences—SGGW, Nowoursynowska 159c, 02-776 Warsaw, Poland
2 Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life
Sciences—SGGW, Nowoursynowska 159c, 02-776 Warsaw, Poland
* Correspondence: [email protected]
Abstract: Recently, plant-based food has become important in the global food market. The increasing
demand for plant-based food is a consequence of the increase in both environmental awareness
and care for animal welfare as well as the changes in nutritional recommendations. Therefore, food
producers are concentrating on fulfilling consumer needs by creating alternatives to animal-based
products with comparable nutritional and sensory qualities but from plant-based sources. One
promising trend is the production of plant-based fish. Thus, this work aimed to summarize the
possibilities of creating plant-based fish analogs, including a review of alternatives to fish products
currently available on the market and the possible use of the various ingredients to produce plant-
based fish analogs like fillets, slices, as well as sticks, or burgers. Furthermore, the plant-based
ingredients were characterized for potential use in fish analogs production. Additionally, the study
includes technologies used for plant-based fish analogs production, e.g., texturization, 3D and 4D
printing, electrospinning, etc. Furthermore, future perspectives were given considering the challenges
and limitations in this range.
Keywords: fish analogs; plant-based products; fish analog markets; texturization; 3D printing
Figure 1. Reasons
Figure for interest
1. Reasons in fish
for interest inanalogs.
fish analogs.
Furthermore,
Plant-based animalananalog
increasing
food part of thecan
products population is giving
be classified up eating
into several fish for as
categories, health
reasons.
follows For example, the contamination of fish by microplastics poses a significant threat.
[15–17]:
Exposure to it, alone or in combination with other pollutants, can lead to various health
issues in fish [9,10]. Furthermore, although fish contains many valuable ingredients and is
an essential protein source for human growth and development, issues related to mercury,
dioxins, microplastics, lead, and arsenic have recently been raised [11,12]. Heavy metals
are a major source of pollution in aquatic environments and can accumulate in the tissues
of fish, posing a risk to human health when consumed [13]. Considering the above reasons,
in the last few years, both large companies and start-ups have tried to meet the needs
of consumers—the Fact.MR report predicts a CAGR increase of the global plant-based
fish market in 2021–2031 by 28%, which means that the projected market value in 2031 is
1.3 billion dollars [14].
Plant-based animal analog food products can be classified into several categories, as
follows [15–17]:
- Dairy analogs. These plant-based products mimic dairy products like milk, cheese,
and yogurt. They are typically made from nuts, soy, chickpea, oats, seeds, tapioca,
yeasts, etc.
Appl. Sci. 2023, 13, 4509 3 of 22
- Meat analogs. These are plant-based products that mimic the meat’s taste, texture,
and nutritional profile. They are usually made from soy, wheat, or pea protein and
can be prepared in different forms, such as burgers, sausages, and meatballs.
- Seafood analogs. These plant-based products are usually made from algae, seaweed,
soy protein, and gluten and can be used in a variety of dishes, such as sushi, crab
cakes, and fish and chips.
- Egg analogs. These are plant-based products that replicate the taste and texture of
eggs. They are typically made from tofu, chickpea flour, or aquafaba.
Commonly, plant-based fish products are a sub-category of seafood analogs and
are specifically designed to mimic the taste, texture, and nutritional profile of fish [16].
Such products usually have a longer shelf life and better food safety than animal-origin
food [18] due to plant-derived protein, eliminating the risk of zoonotic diseases. Moreover,
in order to achieve a meat-like texture, the plant-derived ingredients undergo multiple
processing steps, including heat treatment (high-temperature cooking or extrusion), during
which microbial contamination can be significantly reduced. However, high-temperature
processing of protein products can lead to the formation of harmful substances such as
heterocyclic aromatic amines, polycyclic aromatic hydrocarbons, and advanced glycation
end products from the Maillard reaction. On the other hand, analogs are multi-component,
so each ingredient introduced into the final product (e.g., dyes or spices) can be a source of
pathogenic microflora. Additionally, meals prepared with meat analogs can be more prone
to spoilage during storage due to their neutral pH. This can complicate the supply chain and
requires strict control during release for consumption, compared to their meat-containing
counterparts [19–22].
To prevent contamination, the packaging and storage of meat analogs should be simi-
lar to meat products. Chilled or frozen conditions in closed plastic containers or flexible bags
are recommended for storing plant-based analogs [18]. Additionally, Priyadarshini et al. [23]
noted that the quality of shrimp analog prepared from Pangasionodon hypophthalmus surimi
was better under vacuum packaging than without vacuum (standard packaging). The study
found that psychrophilic microorganisms were detected later in the vacuum-packaged
samples, and the amount of total volatile base nitrogen was lower compared to standard
packaging. Generally, for plant-based analogs packaging with the modified atmosphere can
reduce oxidation and expand shelf life [18]. However, similarly to meat and fish products,
numerous studies have explored the utilization of natural antioxidants to prolong their
shelf life. Thus, it can also be used to prolong the shelf life of plant-based analogs [24].
According to the above-mentioned aspects, this article attempts to compare vegan fish
analogs available on the market, ingredients used for the production of vegan meat and
fish analogs, as well as methods of their preparation.
Waitrose’s Vegan Fishless Fingers. However, they also contain wheat flour, durum wheat
semolina, corn barley flour, wheat gluten, and tapioca starch [40].
Furthermore, Good Catch’s company evaluated their own food additive for plant-
based products, which contains different sources of proteins, such as pea protein isolate,
soy protein concentrate, chickpea flour, faba protein, lentil protein, soy protein isolate, navy
bean powder [27]. Furthermore, to improve the texture of the fish sticks, some companies
use thickener-modified potato starch or xanthan gum, as in Paluszki a’la rybne [37] and
Good Catch’s Fish Sticks [27]. In addition, different sources of oils or fats (olive oil, rapeseed
oil, sunflower oil, and linseed oil) are essential to use in such products because fat is a
carrier of flavor and taste in plant-based analogs [18].
The next type of plant-based fish analog is fish burgers. In comparison to previously
described products, burgers are not as diverse when it comes to their composition. The
main ingredients are water and pea proteins. For example, Salmon Burgers Original and
Whitefish Burgers by Sophie’s Kitchen [36] are made from pea protein, sunflower oil, hemp
oil, potato starch, emulsifier, yeast extract, and spices.
In addition, researchers conducted studies to obtain high-quality products using
different food components. For example, shrimp analogs were obtained from sunflower
oil, alginate, egg white powder, sucrose, glycine, and CaCO3 . Furthermore, shrimp analogs
were incorporated with ethanolic extract of coconut husk as well as cluster bean extract to
extend the shelf-life of ready-to-eat vegan products from 12 days of refrigerated storage to
21 and 16 days, respectively [41].
Type of Name of
Region Producer Ingredients Nutritional Value References
Product the Product
Rice. Water. Flour (wheat). Energy: 690 kJ/165 kcal;
Rapeseed oil. Salt. Thickener Fat: 6.8 g. of which
(methylcellulose). Yeast. Aroma. saturates: 0.5 g;
Breaded Fiber (sugar cane, pea). Buffered Carbohydrates: 19.7 g.
Fish fillets Europe Novish [28]
Fish Fillets vinegar powder (preservative of which sugars: 1.6 g;
ingredient). Modified starch Fibers: 6.6 g; Protein:
(oxidized starch). Food acid (citric 3.1 g; Sodium: 0.46 g;
acid). Spices (paprika powder). Salt: 1.2 g
Water. Breadcrumb Coating (Wheat
Flour. (Flour Calcium Carbonate.
Energy: 568 kJ/136 kcal;
Niacin. Iron. Thiamine). Rapeseed
Fat: 5.7 g. of which
Oil. Water. Salt. Yeast. Konjac
Breaded saturates: 0.4 g;
Flour. Tapioca Starch. Wheat
Fish fillets Fishless Europe Vbites Carbohydrate: 17.8 g. [30]
Starch. Salt. Natural Flavouring.
Steaks of which sugars: 1.1 g;
Sugar. Wheat Fibre. Yeast Extract.
Fibre: 2.7 g; Protein: 2 g;
Thickener: Carrageenan.
Salt: 1.2 g
Preservative: Potassium Sorbate.
Color: Titanium Dioxide.
Energy: 670 kJ/160 kcal;
Water. Soy Protein Concentrate. Fat: 9 g. of which
Canola Oil. Potato Starch. Corn saturates: 0.5 g.
Starch. Methylcellulose. Salt. polyunsaturates: 1.5 g.
Classic
Fish fillets Asia OmniFoods Natural Flavor. Cane Sugar. Yeast monounsaturates: 3 g; [26]
Fillet
Extract. Pea Protein. Rice Protein. Carbohydrate: 9 g.
Shiitake Mushroom Powder. Oats. of which sugars: 1 g;
Wheat Starch. Fiber: 3 g; Protein: 11 g;
Salt: 0.3 g
Appl. Sci. 2023, 13, 4509 6 of 22
Table 1. Cont.
Type of Name of
Region Producer Ingredients Nutritional Value References
Product the Product
Fillets [Water. Soy Protein
Concentrate. Canola Oil. Potato
Starch. Methylcellulose. Salt.
Natural Flavor. Cane Sugar. Yeast Energy: 670 kJ/160 kcal;
Extract. Pea Protein. Rice Protein. Fat: 10 g. of which
Shiitake Mushroom Powder. Oats]. saturates: 0.5 g.
Batter [Water. Enriched polyunsaturates: 1.5 g.
Golden
Fish fillets Asia OmniFoods Unbleached Wheat Flour (Flour. monounsaturates: 3 g; [42]
Fillet
Niacin. Reduced Iron. Thiamine Carbohydrate: 11 g.
Mononitrate. Riboflavin. Folic of which sugars: >1 g;
Acid). Wheat Starch. Cane Sugar. Fiber: 2 g; Protein: 6 g;
Salt. Baking Powder (Sodium Acid Salt: 0.3 g
Pyrophosphate. Sodium
Bicarbonate. Corn Starch.
Monocalcium Phosphate)].
Textured vegetable protein (pea
Energy: 180 kcal; Fat: 8 g.
protein, pea starch). Canola oil.
North of which saturates: 1 g;
Rice flakes (from brown rice).
Plant Based and Sophie’s Carbohydrate: 20 g.
Fish fillets Konjac powder. Seaweed powder. [36]
Fish Fillets South Kitchen of which sugars: 3 g;
Potato starch. Powdered cellulose.
America Protein: 8 g; Fiber: 4.6 g;
Organic agave nectar. Turmeric.
Salt: 0.4 g
White pepper. Sea salt. Ginger.
Water. Soy protein concentrate.
Rapeseed oil. Wheat flour.
Modified corn starch. Tapioca
starch. Potato starch. Wheat gluten.
Methylcellulose. Isolated soy Energy:870 kJ/208 kcal.
North protein. Maltodextrin. Natural Fat: 13.5 g. of which
and flavors. Corn flour. Sea salt. Salt. saturates: 1 g;
Fish fillets Fish Fillets Gardein [43]
South Spices. Onion powder. Yeast Carbohydrate: 12.5 g;
America extract. Yeast. Garlic powder. Soy Fiber: 2.1 g;
flour. Algae oil. Baking powder. Protein: 9.4 g; Salt: 0.4 g
Xanthan gum. Sugar. Seasoning.
Soybean oil. Cane sugar. Citric
acid. Color: Pea protein. Carrot
powder. Beet fiber.
Water. Good Catch 6-Plant Protein
Blend (pea protein isolate. Soy
protein concentrate. Chickpea flour.
Energy: 740 kJ/177 kcal;
Faba protein. Lentil protein. Soy
Fat: 9.7 g. of which
North protein isolate. Navy bean
Plant -Based saturates: 0.9 g;
and Good powder). Wheat flour. Sunflower
Fish fillets Breaded Carbohydrate: 12.4 g. [27]
South Catch oil. Corn starch. Natural flavors.
Fish Fillets of which sugars: 0.9 g;
America Methylcellulose. Yeast extracts.
Fiber: 0.9 g;
Sugar. Salt. Corn maltodextrin.
Protein: 10.6 g
Garlic powder. Onion powder.
Corn flour. Yeast. Spices. Xanthan
gum. Annatto extract. Acetic acid.
Energy: 1071 kJ/257 kcal;
Wheat fiber. Soy fiber. Water. Soy
Fat: 17 g. of which
protein. Soy sauce (soy, wheat, salt,
saturates: 2.8 g;
Vegan Vantastic sugar). Tapioca starch. Sugar. Salt.
Fish fillets Europe Carbohydrate: 17.8 g. [44]
Salmon Foods White pepper. Color: Paprika
of which sugars: 1.1 g;
extract; Seaweed
Fiber: 2.7 g; Protein: 2 g;
(Laminaria japonica).
Salt: 1.2 g
Appl. Sci. 2023, 13, 4509 7 of 22
Table 1. Cont.
Type of Name of
Region Producer Ingredients Nutritional Value References
Product the Product
Energy: 268 kJ/64 kcal;
Fat: 4.5 g. of which
Vegan Soy protein. Wheat protein. Salt.
saturates: 0 g;
Fish fillets Salmon Asia May Wah Soy sauce. Canola oil. Five spice. [32]
Carbohydrate: 3 g. of
Fillet Pepper. Seaweed.
which sugars: 0.5 g;
Protein: 4 g; Salt: 0.3 g
Energy:1381 kJ/330 kcal;
Soybean Protein. Water. Modified Fat: 27 g. of which
TKC
TKC Potato Starch. Soybean Oil. saturates: 5 g;
Fish fillets Vegetarian Asia [33]
Food Seaweed. Sugar. Salt. Carbohydrate: 11 g. of
Cod Fillet
Spices. Seasoning. which sugars: 0 g;
Protein: 12 g; Salt: 0.7 g
Energy: 365 kJ/87 kcal;
Fat: 0.4 g. of which
Isolated soy protein. Textured soy
Vegan Cod saturates: 0.2 g;
Fish fillets Asia Ahimsa protein. Tapioca starch. Vegetable [45]
Fish Carbohydrate: 4 g;
oil. Salt. Sugar. Seaweed.
Protein: 10.5 g; Fiber:
12.2 g; Salt: 0.5 g
Isolated soy protein. Water. Soy. Energy: 812 kJ/194 kcal;
Vegetarian
Fish fillets Asia Everbest Modified tapioca starch. Yeast Protein: 13.2 g; Fat: 11 g; [34]
Goby Fish
extract. Seaweed. Carbohydrate: 11 g
Energy: 546 kJ/123 kcal;
Water. Rapeseed Oil. Soya Protein.
Fat: 9.5 g. of which
Thickeners: Carrageenan. Konjac
Smokey saturates: 0.8 g;
Flour. Potato Starch. Salt. Natural
Slices Salmon Europe VBites Carbohydrate: 5.1 g. of [46]
Flavouring. Onion Powder. Sugar.
Slices which sugars: 0.8 g;
Preservative: Potassium Sorbate;
Fiber: 1.7 g;
Color: Annatto. Iron Oxide.
Protein: 5.6 g; Salt: 1.9 g
Water. Pea protein. Vegetable oils
(rapeseed oil, linseed oil). Flavors. Energy: 401 kJ/96 kcal;
Gelling agent: calcium alginate; Fat: 5.4 g. of which
Revo
thickeners: carrageenan. Konjac; saturates: 0.8 g;
Slices Smoked Europe Revo [35]
Sea salt. Modified starch. Beetroot Carbohydrate: 5.1 g. of
Salmon
concentrate. Vitamins (vitamin B2, which sugars: 0.5 g;
vitamin B6, vitamin B12, vitamin Protein: 5.7 g; Salt: 3 g
D2). Smoke flavor. Color: carotene.
Water. Olive oil. Konjac powder.
Energy: 669 kJ/160 kcal;
Pea starch. Potato starch. Pea
North Fat: 4 g. of which
protein. Sea salt. Organic agave
Smoked and Sophie’s saturates: 0 g;
Slices nectar. Seaweed powder. [47]
Salmon South Kitchen Carbohydrate: 32 g;
Fenugreek. Alginate (from
America Fiber: 6 g; Protein: >2 g.
seaweed). Paprika.
Salt: 0.5 g
Calcium hydroxide.
Water. Breadcrumbs (water,
rapeseed oil, wheat flour, wheat
gluten, salt, corn flour, rice flour,
Energy: 891 kJ/213 kcal;
yeast, olive oil, paprika extract,
Fat: 8.3 g. of which
color: paprika extract). Wheat
saturates: 0.7 g;
Paluszki protein. Oil rapeseed. Isolated soy
Fish Sticks Europe Polsoja Carbohydrates: 21.3 g. of [37]
a’la rybne protein. Salt. Wheat starch. Flavors.
which sugars: 1.8 g;
Wheat fiber. Thickener: Modified
Fiber: 3 g; Protein: 11.5 g;
potato starch. Emulsifier:
Salt: 1.6 g
Methylcellulose. Dried onion juice
concentrate. Dried vegetables
(garlic, carrot, parsley, onion).
Appl. Sci. 2023, 13, 4509 8 of 22
Table 1. Cont.
Type of Name of
Region Producer Ingredients Nutritional Value References
Product the Product
Rehydrated wheat protein. Wheat
flour. Sunflower oil. Water. Spirit Energy: 1029 kJ/245 kcal;
vinegar. Salt. Thickener: Fat: 9.3 g. of which
methylcellulose; natural flavorings. saturates: 1.1 g;
Sea Style Wheat starch. Wheat fibers. Herbs. Carbohydrate: 26 g. of
Fish Sticks Europe Veganz [38]
Sticks Spices. Linseed oil. Potato fibers. which sugars: 1.4 g;
Maltodextrin. Maize Starch. Protein: 13 g; Salt: 1.6 g;
Preservative: Sodium diacetate; Iron: 2.1 g;
Onion powder. Garlic powder. Iron Vitamin B12: 0.38 g
gluconate. Cyanocobalamin.
Tofu (water, soya beans). Water.
Fortified wheat flour (wheat flour,
calcium carbonate, iron, niacin, Energy: 918 kJ/220 kcal;
thiamin). Durum wheat semolina. Fat: 12.7 g. of which
Barley flour. Maize starch. saturates: 1.8 g;
Vegan
Seaweed. Salt. Sunflower oil. Carbohydrate: 13.1 g. of
Fish Sticks Fishless Europe Waitrose [40]
Wheat gluten. Tapioca starch. which sugars: 0.7 g;
Fingers
Lemon zest. Dill. Garlic powder. Fibre: 3.1 g;
Onion powder. Dextrose. Black Protein: 11.8 g;
pepper. Turmeric extract. Salt: 0.65 g
Flavoring. Paprika extract. Maize
maltodextrin. Lemon purée. Yeast.
Reconstituted wheat protein.
Breadcrumbs (wheat flour. Yeast,
salt, paprika powder). Sunflower Energy: 1029 kJ/245 kcal;
oil. Water. Wheat flour. Spirit Fat: 9.3 g. of which
vinegar. Thickener saturates: 1.1 g;
Vegan
(methylcellulose). Natural flavors. Carbohydrate: 26 g. of
Fish Sticks Crispy Europe Vivera [39]
Wheat starch. Wheat fiber. Salt. Sea which sugars: 1.4 g;
Sticks
salt. Linseed oil. Potato fiber. Fiber: 3.2 g; Protein: 13 g;
Maltodextrin. Corn starch. Herbs Salt: 1.6 g; Iron: 2.1 g;
and spices. Preservative (sodium Vitamin B12: 0.38 g
diacetate). Onion powder. Garlic
powder. Iron. Vitamin B12.
Water. Wheat flour. Good Catch
6-Plant protein blend (pea protein
isolate, soy protein concentrate,
Energy: 813 kJ/195 kcal;
chickpea flour, faba protein, lentil
Fat: 9.7 g. of which
North protein, soy protein isolate, navy
saturates: 0.9 g;
Breaded and Good bean powder). Sunflower oil. Corn
Fish Sticks Carbohydrate: 15 g. of [48]
Fish Sticks South Catch starch. Natural flavors.
which sugars: 0.9 g;
America Methylcellulose. Yeast extracts.
Protein: 10.6 g;
Sugar. Corn maltodextrin. Salt.
Fiber: 0.9 g; Salt: 0.4 g
Onion powder. Yeast. Corn flour.
Garlic powder. Spices. Xanthan
gum. Annatto. Acetic acid.
Water. Good Catch 6-Plant Protein
Blend (pea protein isolate, soy protein
Energy: 592 kJ/142 kcal;
concentrate, chickpea flour, faba
Fat: 4.4 g. of which
North protein, lentil protein, soy protein
Fish saturates: 0.4 g;
and Good isolate, navy bean powder). Celery.
Burgers Burgers Carbohydrate: 7.9 g. of [49]
South Catch Green onions. Sunflower oil. Natural
Classic Style which sugars: 0.88 g;
America vegan flavors. Methylcellulose.
Protein: 18.6 g;
Lemon juice. Corn starch. Onion
Fiber: 1.8 g; Salt: 1.8 g
powder. Salt. Yeast extract. Algal oil.
Garlic powder. Spice.
Appl. Sci. 2023, 13, 4509 9 of 22
Table 1. Cont.
Type of Name of
Region Producer Ingredients Nutritional Value References
Product the Product
Water. Pea protein. Safflower oil.
Hempseed oil. Potato starch. Dill. Energy: 740 kJ/177 kcal;
North Contains or less methylcellulose. Fat: 8.8 g. of which
Whitefish and Sophie’s Pea protein isolate. Yeast extract. saturates: 0.9 g;
Burgers [50]
Burgers South Kitchen Salt. Organic cane sugar. Garlic Carbohydrate: 7.1 g. of
America powder. Parsley. Natural flavors. which sugars: 0.9 g;
Lemon juice concentrate. Protein: 16.8 g; Salt: 0.7 g
Lemon oil.
Water. Good Catch 6-Plant Protein
Blend (pea protein isolate, soy
protein concentrate, chickpea flour,
Energy: 740 kJ/177 kcal;
faba protein, lentil protein, soy
Fat: 9.3 g. of which
North protein isolate, navy bean powder).
Salmon saturates: 6.2 g;
and Good Coconut oil. Natural vegan flavors.
Burgers Burgers Carbohydrate: 8.8 g. of [51]
South Catch Sunflower oil. Methylcellulose.
Classic Style which sugars: 0.9 g;
America Yeast extract. Corn starch. Onion
Protein: 14.2 g;
powder. Salt. Lemon juice. Lemon.
Fiber: 1.8 g; Salt: 0.5 g
Orange. Shallot. Spice. Sugar.
Garlic powder. Annatto extract.
Vegetable juice.
Water. Pea protein. Safflower oil.
Hempseed oil. Potato starch. Dill. Energy: 669 kJ/160 kcal;
North Contains or less methylcellulose. Fat: 4 g. of which
Salmon
and Sophie’s Pea protein isolate. Yeast extract. saturates:0.9 g;
Burgers Burgers [52]
South Kitchen Salt. Organic cane sugar. Garlic Carbohydrate: 7.1 g. of
Original
America powder. Vegetable juices (color). which sugars: 1.8 g;
Natural flavors. Lemon juice Protein: 15.9 g; Salt: 0.7 g
concentrate. Lemon oil.
Fish and plant-based analogs are designed to mimic the taste, texture, and appearance
of real fish. However, they differ in their functional properties from animal-origin products.
Fish flesh is composed of water (70–80%), proteins (15–20%), lipids (2–5%), carbohydrates
(2%), minerals, and vitamins [20,25]. Compared to animal-based proteins, plant-based
proteins are easier to produce, but most plant proteins are deficient in essential amino
acids, making them nutritionally incomplete [53]. Therefore, combining plant proteins
from various sources makes it feasible to attain a very high PDCAAS comparable to fish
protein [20]. Nevertheless, the nutritional value of the plant-based products on the market
is far from their animal origin [54]. Figure 3 presents the protein, fats, and carbohydrate
content of salmon and cod [55] and their chosen analogs. Companies try to produce
products that mimic the texture, appearance, and taste of the fish species; however, there is
still a long way to go to reach nutritional levels similar to those of fish species. For example,
salmon (Revo) fish analogs are characterized by low amounts of proteins and fat and high
amounts of carbohydrates. In contrast, cod analogs (TKC Veg Cod Fillet) contain higher
amounts of fats, carbohydrates, and lower protein than medium quantities in fish meat.
Furthermore, fish analogs made from plant-based proteins have higher levels of dietary
fiber. However, plant-based analogs may have lower levels of omega-3 fatty acids, which
are abundant in real fish [25]. Thus, the seafood substitutes are enriched in micronutrients,
such as omega-3 fatty acids and vitamins (A, B, and D), in order to ensure nutritional parity
with traditional seafood products [54].
(TKC Veg Cod Fillet) contain higher amounts of fats, carbohydrates, and lower protein
than medium quantities in fish meat. Furthermore, fish analogs made from plant-based
proteins have higher levels of dietary fiber. However, plant-based analogs may have lower
levels of omega-3 fatty acids, which are abundant in real fish [25]. Thus, the seafood
Appl. Sci. 2023, 13, 4509 substitutes are enriched in micronutrients, such as omega-3 fatty acids and vitamins (A, 10 of 22
B, and D), in order to ensure nutritional parity with traditional seafood products [54].
Figure
Figure 3. Comparison
3. Comparison of fish
of fish andand
fishfish analogs
analogs (salmon
(salmon andand
codcod nutritional
nutritional value—[55],
value—[55], salmon
salmon
analog—Revo, CodCod
analog—Revo, analog—TKC
analog—TKC VegVeg
CodCod
Fillet).
Fillet).
FishFish analogs
analogs mademade from
from plant
plant proteins
proteins havehave a different
a different texture
texture thanthan
realreal
fishfish
duedue to to
the absence of muscle fibers [15]. Various techniques are being
the absence of muscle fibers [15]. Various techniques are being explored for obtaining explored for obtaining
goodgood texture
texture of the
of the plant-based
plant-based analogs;
analogs; however,
however, research
research is still
is still ongoing
ongoing [20].
[20]. Still,
Still, most most
products on the market do not replicate the structure and texture of fish or seafood butbut
products on the market do not replicate the structure and texture of fish or seafood
rather
rather reproduce
reproduce thethe sensory
sensory characteristics
characteristics of processed
of processed fishfish products,
products, including
including their their
appearance, texture, aroma, and flavor [25]. However, fish analogs
appearance, texture, aroma, and flavor [25]. However, fish analogs made from plant-based made from plant-based
proteins
proteins maymayhavehave a slightly
a slightly different
different tastetaste compared
compared to fish
to real real due
fish to
due
thetobitter
the bitter
taste of taste
of some plant-based proteins. Furthermore, the specific taste of the
some plant-based proteins. Furthermore, the specific taste of the plant-based proteins can plant-based proteins
can be masked
be masked and flavored
and flavored to mimictothemimic
tastethe taste of different
of different types
types of fish of Generally,
[53]. fish [53]. Generally,
plant-
based fish analogs and animal-origin products differ in their functional properties,properties,
plant-based fish analogs and animal-origin products differ in their functional which
which is currently
is currently under investigation.
under investigation.
3. The Possible Use of Various Ingredients to Produce Plant-Based Fish Analogs and
Their Characteristics
Separate nutrients such as proteins, carbohydrates, lipids, and whole products such as
legumes can be used to produce plant-based fish alternatives. Fish are a valuable source of
polyunsaturated fatty acids, including omega-3 acids essential for the proper functioning
of the human body. Therefore, vegetable fats rich in omega-3 fatty acids should be used in
plant-based fish replacements. Fish are also characterized by high protein content, which
depends on the species, the conditions of existence, and the type of muscle—it is important
for proteins to be digestible and contain essential amino acids. Fish meat proteins are
distinguished by their high biological value due to the presence of essential amino acids
in a nutritionally favorable proportion. Conversely, vegetable proteins are considered
less valuable. However, proteins from cereals and legumes can be mixed to achieve an
appropriate essential amino acid profile. It is also worth mentioning that vegetable proteins
are usually characterized by good emulsifying, thickening, and foaming properties [15,56].
Appl. Sci. 2023, 13, 4509 11 of 22
3.1. Soya
Soya (Glycine L.) is characterized by its high-fat content, including polyunsaturated
fatty acids. It is also distinguished by its high protein content, which accounts for up to
46% of grains’ total weight. The carbohydrate content, on the other hand, ranges from
approximately 22.8% to 27.9%, and lipids account for a maximum of 23% of the total
weight [57]. There are five principal fatty acids in soybean oil: palmitic (11%), stearic
(4%), oleic (23%), linoleic (54%), and linolenic (8%) [58]. Soy protein has a significant
amino acid composition as it complements cereal protein. Although soy is deficient in
methionine, it contains enough lysine to overcome the lysine deficiency in cereals [59].
For example, the composition of the essential amino acids in defatted soya seed flour
(g/100 g protein) is histidine 2.58; isoleucine 4.45; leucine 7.82; lysine 5.91; threonine 3.61;
tryptophan 0.33; valine 4.58 [60]. Soybean flour, concentrates, and isolates are characterized
by emulsification, fat absorption, viscosity, and water absorption [1]. The high amount of
soy β-conglycinin (7S) is responsible for moisturizing properties, including emulsifying
and foaming. There is a relationship between the proportion of soy glycinin (11S) and soy
β-conglycinin (7S) which affects the gel and foaming properties [61]. Soybean is one of the
leading food and oil crops, growing for over 5000 years. In 2018, the global soybean yield
was 397.9 million tons [62].
3.2. Wheat
Wheat (Triticum L.) is a widely grown cereal [63]. The predominant constituents in
wheat grains are carbohydrates, mainly starch, accounting for 58% of the grains’ dry matter.
The protein content is 11%, while lipids account for 2%. The proteins found in wheat can
be divided into four classes. Storage, metabolic, protective, and proteins have different
functions [64]. Due to its rheological and viscoelastic properties, wheat protein allows for
obtaining the consistency of muscle fibers. However, wheat proteins are allergenic, and
one of the main allergens is the globulin-1 S allele, a glycoprotein [63,65]. Wheat grains are
a valuable source of B vitamins, as well as vitamin E. They contain significant amounts of
potassium, phosphorus, magnesium, and calcium. In addition, they have a nutritionally
favorable ratio of omega-6 to omega-3 acids [64]. The amino acid composition of wheat
lacks essential amino acids such as lysine (1.1%), threonine (1.8%), and methionine (0.7%).
Newly introduced wheat varieties are characterized by increased lysine content [66,67]. In
2018, global wheat production was 762.2 million tons [68].
3.3. Legumes
There is a big group of different proteins based on legumes sources such as peas (Pisum
sativum L.), beans (Phaseolus spp.), chickpeas (Cicer arietinum L.), lentils (Lens culinaris),
lupin (Lupinus albus L.), etc. [69].
The most common and available on the market are pea proteins, characterized by
their high nutritional value. Fresh pea seeds contain 20–50% starch, 17–22% of other car-
bohydrates, 6.2% to 6.5% protein, and 0.4% fat. Pea proteins have no allergenic effect
and are not genetically modified. Pea flour is marked by whipping ability, water, fat
binding, emulsification, gelling, texture property, and foaming [1,70]. Pea proteins are
divided into globulins, albumins, prolamins, and glutelins. Globulin accounts for 55 to
65% of the total protein content. During seed germination, globulin is degraded to legumin
and vicilin. Pea protein is characterized by a high lysine content (6.7 g/100 g protein).
Compared to grain proteins, pea protein is characterized by a high content of leucine
(7.6 g 100 g protein) and phenylalanine (5.7 g/100 g protein) but a lower content of methio-
nine (0.9 g/100 g protein) and cysteine (1.0 g/100 g protein). The content of individual
amino acids also differs between pea protein types. Pea globulin is characterized by a high
content of arginine, while pea albumin contains a high amount of threonine [71,72]. In
2017, the global pea protein market was valued at USD 32.09 million. World production of
dry seeds, according to 2019 FAOSTAT data was about 22 million tons and fresh peas were
about 14.5 million tons [73].
Appl. Sci. 2023, 13, 4509 12 of 22
The common bean (Phaseolus vulgaris L.) is included in the legume group. It is an
important source of protein, as well as energy and dietary fiber. Raw (unprocessed) beans
contain 1.9% lipids, 21.7% protein, and 58.8% carbohydrates. The seeds of this species are
not oilseeds but have a high proportion of unsaturated fatty acids. The primary fatty acids
are linoleic and linolenic (62–83% of total acids). The high proportion of PUFAs in the beans
provides a favorable ratio of omega-3 to omega-6 acids [74]. Among all amino acids, beans
(regardless of the method of pretreatment: autoclaved at 103 kPa, freeze-dried, and also
ground) contain the highest amount of aspartic acid (10.6% in protein) and glutamic acid
(13.6% in protein), as well as significant amounts of essential amino acids, especially leucine
(7.2% in protein), lysine (6.2% in protein), and phenylalanine (5.4% in protein). The grains
also contain small amounts of sulfur amino acids (methionine 0.7% and cysteine 0.3% in
protein), characteristic of legumes. The flour of beans has the same functional properties as
pea flour. Production volume in 2016 in the United States was 140 million tons, of which
114 million tons were directed to consumption [1,60,75].
Chickpea (Cicer arietinum) is one of the most widely grown legumes sold as seeds,
flours, and canned foods. It is a good source of protein (17–22%) that can be extracted
wet and dry. Extruded chickpea protein is rich in essential amino acids such as lysine
(1.45% dry matter), leucine (1.75% dry matter), and phenylalanine (1.29% dry matter).
The limiting amino acid is tryptophan (0.23% dry matter) [76,77]. Chickpeas contain
40–60% low-digestible carbohydrates and 4–8% essential fats. Chickpeas have a higher fat
content than other legumes [78]. The fatty acid composition of chickpeas is 66% PUFA, 19%
MUFA, and 15% SFA. They contain the most linoleic acid (51.2%) and oleic acid (32.6%). In
2018 global chickpea production was 17.19 million tons, of which 27.53% of the crop was
produced in India [79].
Lentils are classed as legumes and are high in protein as well as low in fat. The seeds
of lentils can be black, orange, yellow, or green. The dry matter contains 20.6–31.4% protein.
Lentil proteins are categorized as having a high nutritional value and the right ratios of leucine
to isoleucine and leucine to lysine. These proteins are rich in lysine (6.7 ± 0.6 g/16 g N),
whereas the limiting amino acids are sulfur amino acids (methionine 0.9 ± 0.2 g/16 g N;
cysteine 1.1 ± 0.3 g/16 g N). The content of carbohydrates ranges from 43.4 to 69.9 g per
100 g of dry matter. Lentils also have a high amount of fiber, totaling as much as 26.9 g per
100 g of dry matter. Lentil flour is characterized by similar properties to the flour of peas or
beans. The global production of lentil grains was 63.15 billion tons in 2021 [1,80–82].
Lupin seed has a protein content of 44%. It contains most of the essential amino acids,
with lysine (2.1%) being the most abundant. Lupin seeds are nearly devoid of starch but
contain oligosaccharides, such as raffinose, stachyose, and polysaccharides [66,83]. Lupin
has a relatively low oil content of 18%. The most abundant fatty acids are oleic, palmitic,
linolenic, and erucic acids [70].
3.4. Rice
Rice (Oryza sativa L.) is a cereal rich in carbohydrates, mainly starch. The grain of rice
is characterized by starch amounts to 75–80%. The content of water is 12%, and the content
of protein is only 7%. Still, it is worth mentioning that this protein is highly digestible
and has a high biological value due to its lysine content (about 4%). Rice bran has extra
nutritional compounds, including cellulose, pectin, hemicellulose, lignin, polyphenolics,
vitamin B9, and vitamin E isoforms [84]. Rice is characterized by a low lipid content. Oil
consists mainly of bran, and rice bran oil consists of 36% oleic acid and 37% linoleic acid.
In addition, the content of palmitic acid is considerable and amounts to 18% [85]. Paddy
rice which is hulled, is known as brown rice, whereas white rice is obtained after milling
and polishing. In 2017, rice production totaled about 693 million tons in Asia alone, and
worldwide production was about 770 million tons [86].
Appl. Sci. 2023, 13, 4509 13 of 22
3.5. Corn
Corn (Zea mays, maize) is one of the most important cereal crops in the world. It
exhibits high tolerance to weather conditions. It can be grown in both tropical and temperate
climates. Corn cob is used as a vegetable, and its grains are used as animal feed [87,88]—the
fat content of corn averages 4%. Corn oil contains a high content of linoleic acid. The other
primary fatty acid in corn is oleic acid, which ranges in some corn varieties, like the amount
of linoleic acid [89]. Corn grains contain an average of 7.3% protein. Corn protein–zein
which is one of the primary storage proteins—contains alanine (10%), proline (10%), and
leucine (20%) in the highest amounts [88–90]. Each year, global corn production exceeds 1
billion tons, thus overtaking rice and wheat production by 25%. About 17% of the world’s
corn production undergoes bioconversion to ethanol [91].
Generally, sunflower seeds also contain around 25% oil, but the content has increased to
40% through plant breeding. The main constituents of sunflower oil are linoleic and oleic
acids. The differences in the fatty acid content of sunflower oil are due to the heat treatment
used in its production. It contains approximately 5% palmitic acid, 6% stearic acid, 30%
oleic acid, and 59% linoleic acid. Sunflower seeds that are processed are characterized
by their low carbohydrate content. Furthermore, it is worth mentioning that sunflower
flour can absorb oil, make emulsions, and is characterized by foaming properties and
whipping—but the last property is only as the flour is without chlorogenic acid. In 2018/19,
global sunflower oil production was 51.46 billion tons [1,105].
Rapeseed is another source of protein and contains about 45% protein as well as
around 42.8% fat. Compared to soya and other legumes, rapeseed protein has an excellent
balance of essential amino acids. It is characterized by high levels of sulfur-containing
amino acids, including cysteine and methionine [106]. In turn, the fatty acid profile is as
follows: 12% oleic acid, 13% linoleic acid, 8–9% linolenic acid, 8–9% eicosenoic acid, and
50–51% erucic acid [107]. Rapeseed flour has the same properties as the flour of sunflow-
ers [1]. World rapeseed production in 2018/19 was 70.91 billion tons [105].
ated by the electric potential gradient from the ground electrode. Consequently, the fine
stream exiting the nozzle, shaped like a Tylor cone, becomes a fine fiber. At the same time,
the solvent evaporates and is finally collected on the manifold connected to the electrode.
For electrospinning to occur, the spun proteins must be in an unfolded conformation or
internally unstructured. Vegetable proteins are usually spherical in their native state, so
they should be unfolded (usually using heating) prior to electrospinning, avoiding the
formation of insoluble aggregates. In order to obtain structures resembling animal proteins,
an acidic solution of spirulina protein [18,25,124].
One of the new technologies that can find industrial application in producing meat
and fish analogs is wet spinning [18]. In this process, the protein solution is forced into a
coagulation bath containing a solvent that lowers the solubility of the protein or promotes
cross-linking and fiber formation. The solvent can cause the protein to precipitate, and
with the force of the nozzle, they align the proteins, forming fibers. Binders, e.g., Ca2+ , are
added to the solvent to allow the proteins to cross-link. It is also possible to change the
acidity of the solution, which favors the formation of inter- and intra-molecular chemical
bonds between the protein chains. The products obtained this way are then purified from
the solvent [25,125]. For this technology to be introduced to the food industry, new solvents
are constantly being sought that will be safe for both products and the environment [126].
Therefore, the production of wet spinning fibers also uses raw materials such as soy, wheat,
okra protein, and zein [125].
Directional freezing is based on the use of the freezing process to obtain multilayer
protein structures. The process involves producing microstructures from two-phase systems
(aqueous or organic solutions, suspensions, and slurries). Variable solution concentrations
and the directional freezing rate regulate the shape of the protein structures. In this method,
soy proteins can be used to prepare protein products [127,128].
The shear cell technology is another technique in which a combination of shear and
heat is used to form plant-based meat and fish analogs with layered fibrous structures that
resemble the mouthfeel and texture of real animal products [36]. The shear device used
in this technology is called a shear chamber, where intense shear can be applied. There
are two types of shear cells: conical cells based on cone-plate rheometer and cylindrical
shape—Couette cells, which can be used on an industrial scale. The shear cell technology is
characterized by a lower variation in product quality compared to extrusion. Examples
of proteins that can be subjected to this process are dairy proteins (calcium caseinate), soy
protein isolate, and vital wheat gluten [85,129–131].
plant proteins and other compounds, e.g., oleogels, algae, or fungus, may be used in fish
analogs and affect their quality.
Author Contributions: Conceptualization, M.N., M.T. and K.P.; writing—original draft preparation,
M.N., M.T., P.C., A.P., K.W., A.W., J.P., K.R. and K.P.; writing—review and editing, M.N., M.T. and
K.P.; visualization, M.N.; supervision, M.N.; project administration, M.N. and K.P. All authors have
read and agreed to the published version of the manuscript.
Funding: This study was supported by the Ministry of Education and Science (Poland) from the state
budget within the program “Student research clubs create innovations” in the years 2022–2023 (grant
number SKN/SP/534683/2022).
Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.
Data Availability Statement: Data sharing not applicable.
Conflicts of Interest: The authors declare no conflict of interest.
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