Practice Oxidative Phosphorylation Worksheet11
Practice Oxidative Phosphorylation Worksheet11
Background Material: NADH and FADH2 made during the previous steps of aerobic respiration
of glucose, fatty acids, or amino acids are ultimately transformed into ATP during Oxidative
Phosphorylation in the mitochondria!
Mcatzone.com
https://www.bioscience.org/2009/v14/af/3509/fig5.jpg
Sources Used:
Nelson DL; Cox MM (April 2005). Lehninger Principles of Biochemistry (4th ed.). W. H. Freeman. ISBN 978-0-7167-4339-2.
White D. (September 1999). The Physiology and Biochemistry of Prokaryotes (2nd ed.). Oxford University Press.
ISBN 978-0-19-512579-5.
Overall Equation:
C6H12O6 + 6 O2 6 CO2 + 6 H2O + 38 ATP
ATP PRODUCTION
I. GLYCOLYSIS:
- 2 ATP in energy investment phase
+ 2 ATP
+ 4 ATP in energy pay-off phase *substrate-level phosphorylation
+2 NADH X 3 ATP/NADH = + 6 ATP
WATER PRODUCTION
Each NADH and FADH2 ½ H20 at the end of the ETC
Therefore, for the 10 NADH + 2 FADH2 made = 6 H2O
For each ACETYL-co A ________ NADH X _____ turn X _______ ATP/NADH = _______ ATP
A. Imagine a drug blocks H+ passive diffusion back through protein V, ATP synthase.
How many ATP would each Acetyl-coA make in this scenario? ________________
1 Acetyl-co A turns CAC _____ NADH X _____ turn X _____ ATP/NADH = ________ ATP
How many ATP would each Acetyl-coA make in this scenario? _______________
1 Acetyl-co A turns CAC _____ NADH X _____ turn X _____ ATP/NADH = ________ ATP
C. Imagine a drug damages the inner cell membrane of the mitochondria and causes “pores” to
form between these two domains.
How many ATP would each Acetyl-coA make in this scenario? _________________
1 Acetyl-co A turns CAC _____ NADH X _____ turn X _____ ATP/NADH = ________ ATP
A. Step 1: Fatty Acid Primed (-1 ATP per fatty acid) in cytoplasm
- 1 ATP
Fatty acid + Coenzyme A Fatty acyl-co A (now imported into mitochondria)
Stearic Acid: 18-C fatty acid (use the tally sheet below to determine the number of total ATP
Each cleave produces: _____ NADH X _____ cleaves X _____ ATP/NADH = ________ ATP
_____ FADH2 X _____ cleaves X _____ ATP/FADH2 = ________ ATP
Imagine additional proteins in the electron transport chain evolved in an organism’s mitochondria as
illustrated below. Assume protein I is the site of NADH oxidation, and protein III is the site of FADH 2
oxidation. Also assume that proteins I, II, IV, V and VI “pump” 1 Hydrogen against the concentration
gradient.
A. How many ATP would be generated for the oxidation of 1 Acetyl-co A for this organism?
1 Acetyl-coA turns CAC _____ NADH X _____ turn X _____ ATP/NADH = ________ ATP
B. How many ATP would be generated for the oxidation of 1 Acetyl-co A if treated with a
toxin that blocked NADH binding to protein I, NADH dehydrogenase?
1 Acetyl-co A turns CAC _____ NADH X _____ turn X _____ ATP/NADH = ________ ATP
_____ FADH2 X _____ turn X _____ ATP/FADH2 = ________ATP
_____ ATP X _____ turn = + ________ATP
Total yield = ___________ATP
C. How many ATP would be generated upon the oxidation of 1 Acetyl-co A if treated with a
toxin that blocked protein VII function?
1 Acetyl-coA turns CAC _____ NADH X _____ turn X _____ ATP/NADH = ________ ATP
_____ FADH2 X _____ turn X _____ ATP/FADH2 = ________ATP
_____ ATP X _____ turn = + ________ATP
Total yield = ___________ATP
For each ACETYL-co A __3_NADH X _1___ turn X ___3____ ATP/NADH = ___9____ ATP
A. Imagine a drug blocks H+ passive diffusion back through protein V, ATP synthase.
How many ATP would each Acetyl-coA make in this scenario? 1 ATP (all ATP made from oxidative phosphorylation negated, just
from substrate-level phosphorylation during the CAC)
1 Acetyl-co A turns CAC __3___ NADH X ___1__ turn X ___0__ ATP/NADH = ____0____ ATP
How many ATP would each Acetyl-coA make in this scenario? ____10 ATP (all the ATP made from FADH2 oxidation negated!)
1 Acetyl-co A turns CAC ___3__ NADH X __1___ turn X __3___ ATP/NADH = _____9___ ATP
C. Imagine a drug damages the inner cell membrane of the mitochondria and causes “pores” to form between these two
domains.
How many ATP would each Acetyl-coA make in this scenario? 1 ATP, again if there is no difference in H+ concentration between
the two sides of the mitochondrial matrices, there will be no chemiosmosis specifically
1 Acetyl-co A turns CAC __3___ NADH X ___1__ turn X ___0__ ATP/NADH = ____0____ ATP
Stearic Acid: 18-C fatty acid (use the tally sheet below to determine the number of total ATP
I. Fatty acid priming? (how many ATP needed?) ___- 1 ATP____ ATP
Each cleave produces: __1___ NADH X __8___ cleaves X ___3__ ATP/NADH = ___24_____ ATP
Each Acetyl-co A turns CAC: ___3__ NADH X __9___ turns X __3___ ATP/NADH = ___27_____ ATP
IV. A triglyceride is made up of __3__ fatty acids. Just from the fatty acid components, how
A. How many ATP would be generated for the oxidation of 1 Acetyl-co A for this organism? 19 ATP, If 5 H are “pump”ed per
NADH 5 ATP/NADH; if 3 H+ are “pump”ed per FADH2, then 3 ATP per FADH2
1 Acetyl-coA turns CAC __3___ NADH X __1___ turn X __5___ ATP/NADH = ___15_____ ATP
B. How many ATP would be generated for the oxidation of 1 Acetyl-co A if treated with a toxin that blocked NADH binding to
protein I, NADH dehydrogenase? 4 ATP
1 Acetyl-coA turns CAC __3___ NADH X __1___ turn X __0___ ATP/NADH = ___0_____ ATP
C. How many ATP would be generated upon the oxidation of 1 Acetyl-co A if treated with a toxin that blocked protein VII
function? 1 ATP
1 Acetyl-coA turns CAC __3___ NADH X __1___ turn X __0___ ATP/NADH = ___0_____ ATP