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M SC Medical Biochemistry 2017 18
M SC Medical Biochemistry 2017 18
SYLLABUS FOR
M.SC. MEDICAL BIOCHEMISTRY
( SEMESTER PATTERN )
( For Candidates admitted in the Colleges affiliated to
Periyar University from 2017-2018 onwards )
PERIYAR UNIVERSITY
Illuminate students the principles governing the structure, functional and interaction
of biomolecules. At the same time new revision reflects tremendous development in
Biochemistry. Significantly emphasize is placed on the interrelationship of ideas so
the students could evaluate diagnostic data and provide an expert opinion to the
medical team.
4. At completion of the new Medical Biochemistry curriculum the graduate would have a
thorough knowledge of all aspects of medical biochemistry science relevant to the
discipline, and competences to organize laboratory practice and apply current
techniques, to evaluate the diagnostic data and to provide an expert opinion in the
medical team, or to pursue a career in the fundamental and applied scientific
research. The new concept comprises relevant knowledge in clinical cytology,
microbiology, clinical immunology, blood banking, analytical toxicology, molecular
diagnostics thus concurring with the current trends in Medical Biochemistry and
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M.Sc. MEDICAL BIOCHEMISTRY
Laboratory Medicine.
Provide a working knowledge of Medical Biochemistry that will be applicable for the
practice of Clinical Diagnostic Laboratory and could even run diagnostic lab on their
own.
1) Recognize and explain the functions of the key molecular components and steps of the
synthesis, assembly, and degradation of biological macromolecules;
2) Recall and relate the molecular structures and chemical properties of biological
macromolecules to their functions including ligand/substrate recognition, enzyme
reactions, formation of multi-molecular complexes; and regulation;
3) Relate digestive processes and body production of usable and storable chemical
energy to the chemical composition of foodstuffs, including vitamin and nutrient
requirements;
4) Describe the inputs and outputs of human intermediary metabolism, and relate
mechanisms of metabolic regulation by hormones, feedback loops and other
mechanisms to body organ systems and their demands for energy and metabolites
5) Recognize and explain the molecular basis of major body mechanisms for self-
recognition and self-defense including blood factors, antibodies, anti-oxidants,
hemostasis, and glucose homeostasis;
6) Describe key features and operating principles of the organization of the human
genome, control of gene expression and cell cycle regulation;
SKILLS
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PERIYAR UNIVERSITY
2) Recognize and explain the sources, detection and consequences of genetic defect(s)
underlying diseases;
Attitudes/Behaviors:
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M.Sc. MEDICAL BIOCHEMISTRY
REGULATIONS
1. PREAMBLE AND OBJECTIVES OF THE COURSE:
The syllabus aims to provide the strength to acquire an advanced knowledge and
understanding of the core principles of Biochemistry.
The course for the degree of Master of Science shall consist of two academic years
divided into four semesters.
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PERIYAR UNIVERSITY
4. COURSE OF STUDY:
The course of study for the M.Sc. Degree in the Branch Medical Biochemistry
shall comprise the following subjects according to the syllabus,
ii) Integrated and Skilled Based Elective Course and Non Major Elective Course
subjects
Non major elective course subjects may be chosen by the respective colleges and
the same must be communicated to the University.
SEMESTER I
SEMESTER II
SEMESTER III
SEMESTER IV
5. EXAMINATIONS
There shall be four examinations- two in the first year, two in the second year.
Candidates failing in any subject / subjects will be permitted to appear for such failed
subject / subjects at subsequent examinations. The Syllabus has been divided into
four semesters. Examinations for I and III semesters will be held in November/
December and for II and IV semesters will be held in April / May.
The practical examination I&II will be held in semester-I. Practical III & IV will be held
at the semeste-II. Practical- V & VI will be held in semester –III and in semester-IV
individual Research Project should be performed and dissertation should be and
their report shall be evaluated in viva voce examination conducted before the
examiners at the end of IV - semester.
6. PASSING MINIMUM
A candidate shall be declared to have passed the examination if he /she secure not
less than 50% of the marks in each paper / practical. Candidates who do not secure
the required minimum marks for a pass in a paper / practical shall be required to
appear for and pass the same at a subsequent appearance. For practical, the
minimum for a pass includes the record notebook marks also. There is no passing
minimum for the record notebook. However submission of a record notebook is a
must.
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M.Sc. MEDICAL BIOCHEMISTRY
Candidates who secure not less than 60% of the aggregate marks in the whole
examination shall be declared to have passed the examination in First Class. All
other successful candidates shall be declared to have passed in the Second Class.
Candidates who obtain 75% and above in the aggregate shall be declared to have
passed the examination in First Class with Distinction provided they pass all the
examinations prescribed for the course at the first appearance.
8. RANKING
Candidates who pass all the examinations prescribed for the course in the first
instance and within a period of three academic years from the year of admission to
the course only are eligible for University Ranking.
The maximum duration for completion of the PG Programme shall not exceed
EIGHT semesters.
These regulations shall take effect from the academic year 2017-2018, i.e. for
students who are to be admitted to the first year of the course during the academic
year 2017-2018 and thereafter.
Candidates who were admitted to the PG course of study before 2017-2018 shall be
permitted to appear for the examinations under those regulations for a period of three
years i.e. up to and inclusive of the Examination of April/May 2021. Thereafter, they
will be permitted to appear for the examination only under the regulations then in
force.
SEMESTER IV
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PERIYAR UNIVERSITY
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M.Sc. MEDICAL BIOCHEMISTRY
University
Exam Hours
Instruction
Hrs / Week
Examination
S.No.
Credits
External
Internal
Subject Subject Title
(25%)
(75%)
Total
I SEMESTER
1. Core I CHEMISTRY OF BIOMOLECULES 6 3 25 75 100 4
II SEMESTER
1. Core IV BIOENERGITICS AND INTER 5 3 25 75 100 4
MEDIARY METABOLISM
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PERIYAR UNIVERSITY
University
Exam Hours
Instruction
Hrs / Week
Examination
S.No.
Credits
External
Internal
Subject Subject Title
(25%)
(75%)
Total
III SEMESTER
1. Core VII IMMUNOLOGY 5 3 25 75 100 5
2. Core VIII PHARMACEUTICAL BIO - 5 3 25 75 100 4
-CHEMISTRY AND TOXICOLOGY
3. Core IX CLINICAL AND NUTRITIONAL 5 3 25 75 100 5
BIOCHEMISTRY
4. Elective III BIOSTATISTICS & MEDICAL 4 3 25 75 100 3
BIOINFORMATICS
5. EDC CHOICE OF PAPER SELECTED 4 3 25 75 100 4
BY THE COLLEGE OR DEPARTMENT
6. Core LAB COURSE - V 6 6 40 60 100 3
Practical V
7. Core LAB COURSE - VI 6 6 40 60 100 3
Practical VI
IV SEMESTER
1. Core X BIOMEDICAL INSTRUMNTATION 5 3 25 75 100 5
2. Elective IV MOLECULAR BIOLOGY 4 3 25 75 100 3
AND BIOTECHNOLOGY
3. Project 100 5
4. TOTAL 112 2300 90
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M.Sc. MEDICAL BIOCHEMISTRY
SEMESTER I
UNIT – II
UNIT – III
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PERIYAR UNIVERSITY
and unsaturated fatty acids; Essential and Non essential fatty acids.
Sterols –Structure, Function and Properties of Cholesterol, Bile acids [no structure] ,
and lipoproteins biological production and significance.. Eicosanoids,
Prostaglandins, Thromboxanes, Leukotrienes,
DNA – Watson & Crick Model, A, B and Z forms of DNA.Properties of DNA - byoyant
density, viscosity, chromic effect, Tm, denaturation, renaturation, hybridization and
Cot analysis.Chemical properties
Major classes of RNA – mRNA, rRNA, tRNA, snRNA, hnRNA – structure and biological
functions.
UNIT – V
Vitamins and Minerals : Classification of Vitamins - Fat soluble and water soluble.
Dietary source, structures, RDA, functions and deficiency states. Macro and micro
elements – Dietary source, structures, RDA, functions and deficiency of Iron,
calcium, phosphorus, magnesium, iodine, Zinc and copper.
REFERENCE BOOKS
3. Biochemistry. lubert stryer, 3rd Edn., 1994.W H freeman and co, Sanfrancisco.
7. Text book of biochemistry, Thomas M Devlin, A 1987, 4th edition John Wiley, Inc
publication, New York.
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M.Sc. MEDICAL BIOCHEMISTRY
SEMESTER I
Organ and tissue slice technique, cell disruption and homogenization technique,
Microtomy – Staining and fixation. Cell sorting and cell counting of various tissue
culture collections. Cryopreservation and manometric techniques.
UNIT – II
UNIT – III
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PERIYAR UNIVERSITY
UNIT – IV
Tracer techniques: Radioactive isotopes and half life of isotopes, Principles and
applications of tracer techniques in biology and medical sciences, measurement of
alpha, beta and gamma radiations. Radiation dosimeter, Autoradiography; Principle,
Biological applications. Geiger Muller Counter and Liquid Scintillation counter.
REFERENCES:
1. Principles and techniques of practical Biochemistry, Keith Wilson and John Walker,
1995.Cambridge University Press.
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M.Sc. MEDICAL BIOCHEMISTRY
SEMESTER I
Origin of single cell – theories and concepts. Cell cycle: Prokaryotic and eukaryotic cell
cycle, cell growth and extracellular signal molecular basis of cell cycle regulation, cell
cycle check points, cyclin and cyclin dependent kinases, Apoptosis: Survival and
death facts cell death receptors, cell – cell interactions in cell rescue and death,
erythropoietin in RBC development. Molecular apoptotic events in C. elegans and
mammals, bcl family of proteins, caspases, significance of apoptosis
UNIT-II
Kinetochore architecture and spindle assembly focal adhesion points,. Major types of
cell adhesion molecules (CAMs) – Cadherin, Integrins, Selectins and super family
Immunoglobulin
UNIT-III
UNIT IV
Composition of Cell membrane: Lipid Bilayer, Peripheral and Integral proteins. Fluid
mosaic model. Membrane transport types: Uniport, Synport,Antiport. Active
transport: P-types[Na+K+ATPases, F-Type ATPases (ATP synthetases),Ionophores,
Ion channels and ligand/ voltage gated channels
Protein sorting: Golgi and endoplasmic reticulum and lysosome complex in protein
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PERIYAR UNIVERSITY
UNIT V
Cancer Biology: Etiological factors, primary, secondary tumors benign and malignant
tumors. Oncogene: proto oncogenes and viral oncogenes, oncogene activation, tumor
suppressor genes, DNA tumorviruses, tumor specific antigens and tumor evasion.
Metastasis: Molecular events in migration, extravasation, chemokines, role of ECM in
metastasis. Angiogenesis: angiogenetic and antiangiogenetic factors, vasculogenesis.
Types of cancer cells and morphological alterations
REFERENCE BOOKS
2. Molecular Biology of cells, B. Alberts, Alexander Johnson, ,Julian Lewis, Martin Raff,
Keith Roberts, Peter Walter,5thEdn. 2008. G.S. Garland Sciences, Taylor & Francis
Group,New york
6. Cell and Molecular Biology 3 Edn. Gerald Karp, John Wiley & Sons Inc.
rd
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M.Sc. MEDICAL BIOCHEMISTRY
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PERIYAR UNIVERSITY
UNIT IV
Digestive system: Secretion of digestive juices, digestion and assimilation of
Carbohydrates, Proteins Fats and vitamins. Gastro intestinal hormones.
Nervous system: Structure of neuron, resting potential and action potential,
Propagation of nerve – impulses, Structure of synapse, synaptic transmission
(electrical and chemical theory). Structure of Neuro muscular junction and
mechanism of neuro muscular transmission, Second messengers,Neuro
transmitters.
UNIT V
Reproductive systems
Function of reproductive system. Male reproductive system - functions of testis,
spermatogenesis site and stage factors influencing semen, Endocrine functions of
testis, Androgens - Testosterone - structure and functions.
Female Reproductive system - Ovulation, Menstrual cycle, physiological changes
during pregnancy - Actions of oestrogen, progesterone, functions of placenta.
Lactation - Composition of milk and factors controlling lactation
REFERENCE BOOKS
1. Human Physiology – Volume I & II, Chatterjee, C.C - 11 edition, 1992.
th
5. Agarwal physiological T.B. of Biochemistry ,Agarwal G.R & Agarwal B.P. Chemistry.
6. Harper's Biochemistry, Murray. R.G. et al., 2009, 24 edition.
th
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M.Sc. MEDICAL BIOCHEMISTRY
SEMESTER I
3. Microtomy
4. Histochemical Techniques
6. Cell Fractionation
REFERENCE BOOKS
Publications
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PERIYAR UNIVERSITY
SEMESTER I
REFERENCE BOOKS
Publications
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M.Sc. MEDICAL BIOCHEMISTRY
UNIT II
Carbohydrate Metabolism
Introduction to metabolism of cells, glycolysis and its regulation, citric acid cycle, its
function in energy generation and regulation of TCA cycle, Gluconeogenesis and its
regulation, Metabolism of glycogen and its regulation. Hexose Monophosphate
Pathway, Uronic acid pathway, Cori cycle, Metabolism of other hexoses – Fructose,
Galactose. Hormonal influence and regulation of Carbohydrate metabolism.
UNIT III
Lipid Metabolism :
Biosynthesis of Fatty acid - Palmitic acid, Stearic acid ,Oleic acid, linoleic acid and
Arachidonic acid, Oxidation of saturated and unsaturated fatty acids. Oxidation of
fatty acids-alpha ,beta and omega oxidation in even and odd numbered fatty acids.
Metabolism of Triacyl glycerol, phospholipids and sphingolipids. Cholesterol
biosynthesis and regulation. Catabolism transport and excretion of cholesterol,
lipoprotein metabolism. Ketone bodies formation and utilization.
UNIT IV
Aminoacid Metabolism
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PERIYAR UNIVERSITY
UNIT V
Nucleotide Metabolism
REFERENCE BOOKS
3. Outlines of Biochemistry, Eric E.Conn, P.K. Stumpf, G.Brueins and Ray H.Doi, 1987.
John Wiley & Sons, NY
4. Biochemistry, Lubert stryer, 1994. 3rd Edn., W H freeman and co, Sanfrancisco.
8. Harper's Biochemistry, R.K. Murray and others, 25 ed 2009. Appleton and Lange,
th
Stanford
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M.Sc. MEDICAL BIOCHEMISTRY
SEMESTER II
IUB system of classification and nomenclature, Enzyme units , Active site, Enzyme
Kinetics-Activation energy, Derivation of Michaelis Menten equation, Factors
affecting enzyme activity, Enzyme assay, Coenzymes, Isoenzymes and mutlienzyme
complex, Mechanism and regulation of enzyme action- Allosteric and feedback
regulation.
UNIT II
UNIT III
UNIT IV
UNIT V
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PERIYAR UNIVERSITY
REFERENCE BOOKS
1. Enzymes By Dixon , E.C Webb, CJR Thorne and K.F. Tipton, Longmans , London.
4. Protein Biotechnology, Gary Walsh and Denis Headon, 1994.John Wiley and Sons,
5. Protein Biochemistry and Biotechnology, Gary Walsh,2002, John Wiley and Sons
Ltd.
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M.Sc. MEDICAL BIOCHEMISTRY
SEMESTER II
Cell surface and intracellular (cytoplasmic and nuclear) receptors, G protein coupled
receptors, Pharmacological receptors – Neurotransmitter receptors. Second
messenger system – Ca cAMP, cGMP, DAG, and IP3.
2+
UNIT II
UNIT III
UNIT IV
UNIT V
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PERIYAR UNIVERSITY
REFERENCE BOOKS :
Publishing Co.
5. Harpers Biochemistry, Murray et al., 2003. 2nd Edition, Mc Graw Hill Publications,
USA.
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M.Sc. MEDICAL BIOCHEMISTRY
SEMESTER II
UNIT - II
Medical Bacteriology
UNIT -III
Medical Mycology
UNIT – IV
Medical Parasitology
Morphology, life cycle, pathogenicity, lab diagnosis treatment and control measures
of Intestinal amoebae – Entamoeba histolytica. Intestinal and genital flagellates –
Giardia intestinalis and Trichomonas vaginalis. Blood flagellates – Trypanosoma.
Haemosporina –Plasmodium. Coccidian – Toxoplasma gondii. Helminthic parasites –
Cestodes – Taenia solium. Trematode – Fasciola hepatica. Nematodes – Ascaris
lumbricoidesand Wuchereria bancrofti
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PERIYAR UNIVERSITY
UNIT – V
Medical Virology
REFERENCE BOOKS
2. Medical Virology Morag C and Timbury, M.C 1994. 10 Edition. Churchill Livingston,
th
London
5. A text book of Medical Mycology , Jegadish Chander ,1996. Interprint. New Delhi
6. Text book of Medical Parasitology Protozoology and Helminthology Text and colour
Atlas.Subash Chandra Parija 2013. 4 Edition. All India Publishers and Distributers,
th
New Delhi.
7. Medical Microbiology and Immunology. Warren Livingstone and Ernest Jawetz 2000.
6 Edition. McGraw Hill companies. Inc.
th
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M.Sc. MEDICAL BIOCHEMISTRY
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M.Sc. MEDICAL BIOCHEMISTRY
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PERIYAR UNIVERSITY
hybrid lymphoid cell lines. Protein labelling techniques. Antigen - Antibody reactions
– Agglutination and precipitation, Immuno-electrophoresis, Immuno - blotting
technique, RIA, ELISA - principle, types and applications. Immuno-fluorescence,
Avidin-biotin mediated assay, Flow cytometry.
REFERENCE BOOKS
1. Kuby Immunology,Thomas J. Kindt, Richard A Goldsby, 2013. 7 Edn., Publisher W
th
H Freeman & Co
2. Roitt's Essential Immunology- Ivan Roitt, Peter Delves, et al., 2011, 12 Edition,
th
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M.Sc. MEDICAL BIOCHEMISTRY
SEMESTER III
UNIT II
UNIT III
UNIT IV
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PERIYAR UNIVERSITY
UNIT V
REFERENCE BOOKS
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M.Sc. MEDICAL BIOCHEMISTRY
SEMESTER III
Specimen: Whole blood, plasma, serum, CSF and gastric fluid collection methods and
preservation
UNIT II
UNIT III
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PERIYAR UNIVERSITY
UNIT IV
UNIT V
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M.Sc. MEDICAL BIOCHEMISTRY
5. Modern Nutrition in health and disease, Robert S Goodhart, 2012, 11th edition,
Lippincott Williams and Wilkins.
6. Food facts and principles, N Shakuntala, O Manay, 2001, New Age International Pvt
Ltd.
7. Practical Clinical Biochemistry , Harold Varley, 2006. 4th and 6th editions, CBS
publishers
8. Clinical Chemistry in diagnosis and treatment, Mayne,1999, ELBS,
9. Clinical Biochemistry- Metabolic and clinical aspects, William J.Marshall, Marta
Lapsley,Andrew P. Day, Ruth M. Ayling,2014. Churchill Livingstone, Elsevier.
10. Food Science , B. Srilakshmi,2003.3rd Edn. New Age International
11. Modern Nutrition in health and disease, Robert S Goodhartand Maurice E.
Shils,1974.5th Edn. Lea & Febiger US
12. Food facts and principles, Sakuntala N.Manay and M. Shadaksharaswamy;
2009.3rd Edition New Age International
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PERIYAR UNIVERSITY
SEMESTER III
Organizing a statistical survey, Planning and executing the survey. Source of data -
Primary and secondary data collection. Classification and tabulation of data.
Diagrammatic and graphic presentation of data.
UNIT II
Measure of central tendency - arithmetic mean, median, mode, quartiles, deciles and
percentiles. Measure of variation - range, quartile deviation, mean deviation,
standard deviation, Coefficient of variation. Correlation analysis - Scatter diagram,
Karl's Pearson's coefficient of correlation and Spearman's rank method. Regression
analysis.
UNIT III
UNIT IV
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M.Sc. MEDICAL BIOCHEMISTRY
UNIT V
REFERENCE BOOKS
5. Biochemical calculation and biostatistics, Dr. E.Padmini, 2010.2 Edn. Wiley India
nd
Pvt.Limited
11. An Introduction to Computational Biochemistry, C.S. Tsai, 2002. Wiley& Liss, New
York.
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PERIYAR UNIVERSITY
SEMESTER III
2. Differential count
3. Estimation of Haemoglobin
a) Colorimetric method
b) Sahli's method
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M.Sc. MEDICAL BIOCHEMISTRY
3. Estimation of Phosphate
4. Estimation of Na & K, Ca
REFERENCE BOOKS
3. Text book of Practical Biochemistry, David T.Plummer, 2010. 3 Edn., Book Vistas,
rd
NewDelhi
Publishers.
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PERIYAR UNIVERSITY
SEMESTER III
2. RA test
3. CRP test
4. Pregnancy Test
II. Precipitation
2. Immuno Electrophoresis
5. Immunoprecipitation test
1. ELISA
1. Western Blotting
REFERENCE BOOKS
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M.Sc. MEDICAL BIOCHEMISTRY
SEMESTER IV
UNIT - II
UNIT - III
UNIT - IV
UNIT - V
44
PERIYAR UNIVERSITY
REFERENCE BOOKS :
Publishing Company.
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M.Sc. MEDICAL BIOCHEMISTRY
UNIT - II
UNIT - III
UNIT - IV
Animal cell culture: Culture media – role of carbon dioxide, serum, growth factors,
glutamine in cell culture. Types of cell culture – primary and established culture,
organ culture, tissue culture. Disaggregation of tissue and primary cell culture, cell
separation, cryopreservation..
.UNIT - V
Transgenic animals and plants – monoclonal and polyclonal antibodies – vaccines and
diagnosis – edible vaccines – humulins- interferons
Stem cells – History – types- culturing of stem cells – Embyonic stem cells, cord blood,
adult stem cells- cloning- stem cell banking – Stem cell therapy- Ethics.
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PERIYAR UNIVERSITY
REFERENCE BOOKS
3. Cell and Molecular Biology, 2000. 3 edn. Gerald Karp, John Wiley and Sons Inc
rd
4. Molecular Cell Biology – Lodish, Baltimore et al., 1995, Scientific American Book,.
7. Molecular Biotechnology. Glick B.R. and Pasternak J.J. 2010,4 Edn. ASM Press,
th
USA.
9. Cloning 1 and 2, Glover D.M. and Hames B.D. 1995 IRL Press .
10. Recombinant DNA ,J.Watson, 1992 2 edition, W.H, Freeman and Co., NY.
nd
11. Essential Molecular Biology A Practical approach, T.A. Brown, 2007. 2 edition, IRL
nd
Press, Oxford.
12. Gene Cloning, An introduction, T.A. Brown 1995, 3 Edn. Chapman and Hall.
rd
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M.Sc. MEDICAL BIOCHEMISTRY
UNIT - II
UNIT - III
Fatty acids - source of fats and importance of fats and lipids in living organism and.
Role of LDL, VLDL, HDL and chylomicrons in human body. Normal levels of
cholesterol hypercholesterolemia and role of cholesterol in Blood pressure.
Atherosclerosis and Heart attack' Prevention and control of heart related diseases
UNIT – IV
Water structure, physical and chemical properties. Vitamins- water soluble and fat
soluble vitamins; Sources, chemical composition, Biological function and property,
Deficiency diseases in human.
UNIT - V
REFERENCE BOOKS
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PERIYAR UNIVERSITY
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M.Sc. MEDICAL BIOCHEMISTRY
Digestive system: outline of digestive system. Buccal, gastric and Intestinal digestion.
Role of liver in digestion. Intestinal- Absorption and assimilation- hormonal influence
in digestion.
UNIT - II
UNIT - III
Nervous and Muscular system: outline of nervous system, structure of neuron, types
of neuron, neurotransmitters. Condition of nerve impulse transmission, synaptic
transmission. Muscle –Types of muscle- Role of Actin and Myosin.-Action Potential.
Neuro muscular junction. Reflex action.
UNIT – IV
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PERIYAR UNIVERSITY
UNIT - V
REFERENCE BOOKS
1. Food Science ,Norman N.Potter, 1986. 3 edn. .Springer Science LLC, NY.
rd
publications, Tokyo.
3. Text Book of Medical Biochemistry ,M.N, Chatterjea and Rana Shindhe 2002. 5 th
4 Food facts and principles, 1987. Shakuntala Manay , John Wley and Sons.
5 Modern nutrition in Health and disease , Robert S. Goodhart, Maurice, E. Shils, 1980.
Michael G. Wohl, Robert S. Goodhart and Maurice E. Shils (Editor).
6 Review of Medical Physiology by Ganong .W.F .2016, 25 Edition, A& L Lange series.
th
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M.Sc. MEDICAL BIOCHEMISTRY
UNIT - II
UNIT - III
UNIT - IV
UNIT - V
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PERIYAR UNIVERSITY
REFERENCE BOOKS
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