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Draft Microbio Syllabus Panda
Draft Microbio Syllabus Panda
F
O
R
THREE-YEAR HONOURS
DEGREE COURSE OF STUDIES
MICROBIOLOGY
2018
Structure of B. Sc. Honours Microbiology under CBCS
Core Course
C-1: Introduction to Microbiology and Microbial Diversity
C-2: Bacteriology
C-3: Biochemistry
C-4: Cell Biology
C-5: Virology
C-6: Microbial Physiology and Metabolism
C-7: Molecular Biology
C-8: Microbial Genetics
C-9: Environmental Microbiology
C-10: Recombinant DNA Technology
C-11: Food and Dairy Microbiology
C-12: Industrial Microbiology
C-13: Immunology
C-14: Medical Microbiology
Discipline Specific Elective (Any Four)
DSE-1: Bioinformatics
DSE-2: Microbial Biotechnology
DSE-3: Advances in Microbiology
DSE-4: Plant Pathology
DSE-5: Biomathematics and Biostatistics
DSE-6: Inheritance Biology
DSE-7: Microbes in Sustainable Agriculture and Development
DSE-8: Biosafety and Intellectual Property Rights
DSE-9: Instrumentation and Biotechniques
DSE-10: Project Work
Skill Enhancement Elective Courses (Any Two)
SE-1: Microbial Quality Control in Food and Pharmaceutical Industries
SE-2: Microbial Diagnosis in Health Clinics
SE-3: Biofertilizers and Biopesticides
SE-4: Food Fermentation Techniques
SE-5: Management of Human Microbial Diseases
SE-6: Microbiological Analysis of Air and Water
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-1: INTRODUCTION TO MICROBIOLOGY AND MICROBIAL DIVERSITY
(THEORY)
SEMESTER –I
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Tortora GJ, Funke BR and Case CL. (2008). Microbiology: An Introduction. 9th edition.
Pearson Education
2. Madigan MT, Martinko JM, Dunlap PV and Clark DP. (2014). Brock Biology of Microorganisms.
14th edition. Pearson International Edition
3. Cappucino J and Sherman N. (2010). Microbiology: A Laboratory Manual. 9th edition.
Pearson Education Limited
th
4.Wiley JM, Sherwood LM and Woolverton CJ. (2013) Prescott’s Microbiology. 9 Edition.
McGraw Hill International.
5. Atlas RM. (1997). Principles of Microbiology. 2nd edition. WM.T.Brown Publishers.
6. Pelczar MJ, Chan ECS and Krieg NR. (1993). Microbiology. 5th edition. McGraw Hill
Book Company.
7. Stanier RY, Ingraham JL, Wheelis ML, and Painter PR. (2005). General
Microbiology. 5th edition. McMillan.
B.Sc (HONOURS) MICROBIOLOGY (CBCS
STRUCTURE) C-2: BACTERIOLOGY (THEORY)
SEMESTER –I
TOTAL HOURS: 60 CREDITS: 4
Unit 3 Microscopy No. of Hours: 6 Bright Field Microscope, Dark Field Microscope, Phase
Contrast Microscope, Fluoresence Microscope, Confocal microscopy, Scanning and
Transmission Electron Microscope
SUGGESTED READINGS
1. Atlas RM. (1997). Principles of Microbiology. 2nd edition. WM.T.Brown Publishers.
2. Black JG. (2008). Microbiology: Principles and Explorations. 7th edition. Prentice Hall
th
3. Madigan MT, and Martinko JM. (2014). Brock Biology of Micro-organisms. 14 edition. Parker
J. Prentice Hall International, Inc.
4. Pelczar Jr MJ, Chan ECS, and Krieg NR. (2004). Microbiology. 5th edition Tata McGraw Hill.
5. Srivastava S and Srivastava PS. (2003). Understanding Bacteria. Kluwer Academic
Publishers, Dordrecht
6. Stanier RY, Ingraham JL, Wheelis ML and Painter PR. (2005). General Microbiology. 5th
edition McMillan.
th
7. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An Introduction. 9 edition
Pearson Education.
th
8. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9
edition. McGraw Hill Higher Education.
9. Cappucino J and Sherman N. (2010). Microbiology: A Laboratory Manual. 9th edition. Pearson
Education Limited
B.Sc (HONOURS) MICROBIOLOGY (CBCS
STRUCTURE) C-3: BIOCHEMISTRY (THEORY)
SEMESTER –II
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Campbell, MK (2012) Biochemistry, 7th ed., Published by Cengage Learning
2. Campbell, PN and Smith AD (2011) Biochemistry Illustrated, 4th ed., Published by
Churchill Livingstone
3. Tymoczko JL, Berg JM and Stryer L (2012) Biochemistry: A short course, 2nd ed., W.H.Freeman
4. Berg JM, Tymoczko JL and Stryer L (2011) Biochemistry, W.H.Freeman and Company
5. Nelson DL and Cox MM (2008) Lehninger Principles of Biochemistry, 5th Edition., W.H.
Freeman and Company,
6. Willey MJ, Sherwood, LM & Woolverton C J (2013) Prescott, Harley and Klein’s
Microbiology by. 9th Ed., McGrawHill
rd
7. Voet,D. and Voet J.G (2004) Biochemistry 3 edition, John Wiley and Sons,
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-4: CELL BIOLOGY (THEORY)
SEMESTER –II
TOTAL HOURS: 60 CREDITS: 4
Unit 5 Cell Cycle, Cell Death and Cell Renewal No. of Hours: 12
Eukaryotic cell cycle and its regulation, Mitosis and Meiosis
Development of cancer, causes and types
Programmed cell death
Stem cells
Embryonic stem cell, induced pleuripotent stem cells
SUGGESTED READING
1. Hardin J, Bertoni G and Kleinsmith LJ. (2010). Becker’s World of the Cell. 8th edition. Pearson.
2. Karp G. (2010) Cell and Molecular Biology: Concepts and Experiments. 6th edition. John Wiley
& Sons. Inc.
3. De Robertis, EDP and De Robertis EMF. (2006). Cell and Molecular Biology. 8th edition.
Lipincott Williams and Wilkins, Philadelphia.
4. Cooper, G.M. and Hausman, R.E. (2009). The Cell: A Molecular Approach. 5th Edition.
ASM Press & Sunderland, Washington, D.C.; Sinauer Associates, MA.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-5: VIROLOGY (THEORY)
SEMESTER –III
TOTAL HOURS: 60 CREDITS: 4
Unit 3 Viral Transmission, Salient features of viral nucleic acids and Replication
No. of Hours: 20
Modes of viral transmission: Persistent, non-persistent, vertical and horizontal
Salient features of viral Nucleic acid : Unusual bases (TMV,T4 phage), overlapping genes (ɸX174,
Hepatitis B virus), alternate splicing (HIV), terminal redundancy (T4 phage), terminal cohesive ends
(lambda phage), partial double stranded genomes (Hepatitis B), long terminal repeats (retrovirus),
segmented (Influenza virus), and non-segmented genomes (picornavirus), capping and tailing
(TMV) Viral multiplication and replication strategies: Interaction of viruses with cellular receptors
and entry of viruses. Replication strategies of viruses as per Baltimore classification (phi X 174,
Retroviridae, Vaccinia, Picorna) , Assembly, maturation and release of virions
1. Study of the structure of important animal viruses (rhabdo, influenza, paramyxo hepatitis B
and retroviruses) using electron micrographs
2. Study of the structure of important plant viruses (caulimo, Gemini, tobacco ring spot,
cucumber mosaic and alpha-alpha mosaic viruses) using electron micrographs
3. Study of the structure of important bacterial viruses (ɸX 174, T4, λ) using electron micrograph.
4. Isolation and enumeration of bacteriophages (PFU) from water/sewage sample using double
agar layer technique
5. Studying isolation and propagation of animal viruses by chick embryo technique
6. Study of cytopathic effects of viruses using photographs
7. Perform local lesion technique for assaying plant viruses.
SUGGESTED READING
1. Dimmock, NJ, Easton, AL, Leppard, KN (2007). Introduction to Modern Virology. 6th
edition, Blackwell Publishing Ltd.
2. Carter J and Saunders V (2007). Virology: Principles and Applications. John Wiley and Sons.
3. Flint SJ, Enquist, LW, Krug, RM, Racaniello, VR, Skalka, AM (2004). Principles of Virology,
Molecular biology, Pathogenesis and Control. 2nd edition. ASM press Washington DC.
4. Levy JA, Conrat HF, Owens RA. (2000). Virology. 3rd edition. Prentice Hall publication,
New Jersey.
5. Wagner EK, Hewlett MJ. (2004). Basic Virology. 2nd edition. Blackwell Publishing.
6. Mathews. (2004). Plant Virology. Hull R. Academic Press, New York.
7. Nayudu MV. (2008). Plant Viruses. Tata McGraw Hill, India.
8. Bos L. (1999) Plant viruses-A text book of plant virology by. Backhuys Publishers.
9. Versteeg J. (1985). A Color Atlas of Virology. Wolfe Medical Publication.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-6: MICROBIAL PHYSIOLOGY AND METABOLISM
(THEORY) SEMESTER –III
TOTAL HOURS: 60 CREDITS: 4
Unit 1 Microbial Growth and Effect of Environment on Microbial Growth No. of Hours: 12
Definitions of growth, measurement of microbial growth, Batch culture, Continuous culture,
generation time and specific growth rate, synchronous growth, diauxic growth curve
Microbial growth in response to environment -Temperature (psychrophiles, mesophiles,
thermophiles, extremophiles, thermodurics, psychrotrophs), pH (acidophiles, alkaliphiles), solute
and water activity (halophiles, xerophiles, osmophilic), Oxygen (aerobic, anaerobic,
microaerophilic, facultative aerobe, facultative anaerobe),barophilic.
Microbial growth in response to nutrition and energy – Autotroph/Phototroph,
heterotrophy, Chemolithoautotroph, Chemolithoheterotroph, Chemoheterotroph,
Chemolithotroph, photolithoautotroph, Photoorganoheterotroph.
1. Study and plot the growth curve of E. coli by turbidometric and standard plate count methods.
2. Calculations of generation time and specific growth rate of bacteria from the graph plotted with
the given data
3. Effect of temperature on growth of E. coli
4. Effect of pH on growth of E. coli
5. Effect of carbon and nitrogen sources on growth of E.coli
6. Effect of salt on growth of E. coli
7. Demonstration of alcoholic fermentation
8. Demonstration of the thermal death time and decimal reduction time of E. coli.
SUGGESTED READINGS
1. Madigan MT, and Martinko JM (2014). Brock Biology of Microorganisms. 14th edition.
Prentice Hall International Inc.
2. Moat AG and Foster JW. (2002). Microbial Physiology. 4th edition. John Wiley & Sons
3. Reddy SR and Reddy SM. (2005). Microbial Physiology. Scientific Publishers India
4. Gottschalk G. (1986). Bacterial Metabolism. 2nd edition. Springer Verlag
6. Stanier RY, Ingrahm JI, Wheelis ML and Painter PR. (1987). General Microbiology. 5th
edition, McMillan Press.
7. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th
edition. McGraw Hill Higher Education.
B.Sc (HONOURS) MICROBIOLOGY (CBCS
STRUCTURE) C-7: MOLECULAR BIOLOGY (THEORY)
SEMESTER –III
TOTAL HOURS: 60 CREDITS: 4
DNA Structure: Miescher to Watson and Crick- historic perspective, DNA structure, Salient
features of double helix, Types of DNA, Types of genetic material, denaturation and
renaturation, cot curves. DNA topology - linking number, topoisomerases; Organization of
DNA Prokaryotes,Viruses, Eukaryotes.RNA Structure, Organelle DNA -- mitochondria and
chloroplast DNA.
1. Study of different types of DNA and RNA using micrographs and model /
schematic representations
2. Study of semi-conservative replication of DNA through micrographs /
schematic representations
3. Isolation of genomic DNA from E. coli
4. Estimation of salmon sperm / calf thymus DNA using colorimeter (diphenylamine
reagent) or UV spectrophotometer (A260 measurement)
5. Estimation of RNA using colorimeter (orcinol reagent) or UV
spectrophotometer (A260 measurement)
6. Resolution and visualization of DNA by Agarose Gel Electrophoresis.
7. Resolution and visualization of proteins by Polyacrylamide Gel Electrophoresis (SDS-PAGE).
SUGGESTED READINGS
1. Watson JD, Baker TA, Bell SP, Gann A, Levine M and Losick R (2008) Molecular Biology of
the Gene, 6th edition, Cold Spring Harbour Lab. Press, Pearson Publication
2. Becker WM, Kleinsmith LJ, Hardin J and Bertoni GP (2009) The World of the Cell, 7th
edition, Pearson Benjamin Cummings Publishing, San Francisco
3. De Robertis EDP and De Robertis EMF (2006) Cell and Molecular Biology, 8th edition.
Lippincott Williams and Wilkins, Philadelphia
4. Karp G (2010) Cell and Molecular Biology: Concepts and Experiments, 6th edition, John Wiley
& Sons. Inc.
th
5. Sambrook J and Russell DW. (2001). Molecular Cloning: A Laboratory Manual. 4 Edition,
Cold Spring Harbour Laboratory press.
6. Krebs J, Goldstein E, Kilpatrick S (2013). Lewin’s Essential Genes, 3rd Ed., Jones and
Bartlett Learning
7. Gardner EJ, Simmons MJ, Snustad DP (2008). Principles of Genetics. 8th Ed. Wiley-India
B.Sc (HONOURS) MICROBIOLOGY (CBCS
STRUCTURE) C-8: MICROBIAL GENETICS (THEORY)
SEMESTER –IV
TOTAL HOURS: 60 CREDITS: 4
Unit 4 Phage Genetics No. of Hours: 8 Features of T4 genetics , Genetic basis of lytic versus
lysogenic switch of phage lambda
1. Analysis of soil - pH, moisture content, water holding capacity, percolation, capillary action.
2. Isolation of microbes (bacteria & fungi) from soil (28ºC & 45ºC ).
3. Isolation of microbes (bacteria & fungi) from rhizosphere and rhizoplane.
4. Assessment of microbiological quality of water.
5. Determination of BOD of waste water sample.
6. Study the presence of microbial activity by detecting (qualitatively) enzymes
(dehydrogenase, amylase, urease) in soil.
7. Isolation of Rhizobium from root nodules.
SUGGESTED READINGS
1. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications. 4th
edition. Benjamin/Cummings Science Publishing, USA
2. Madigan MT, Martinko JM and Parker J. (2014). Brock Biology of Microorganisms. 14th
edition. Pearson/ Benjamin Cummings
3. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2nd edition,
Academic Press
4. Okafor, N (2011). Environmental Microbiology of Aquatic & Waste systems. 1st edition,
Springer, New York
5. Singh A, Kuhad, RC & Ward OP (2009). Advances in Applied Bioremediation. Volume
17, Springer-Verlag, Berlin Hedeilberg
6. Barton LL & Northup DE (2011). Microbial Ecology. 1st edition, Wiley Blackwell, USA
Campbell RE. (1983). Microbial Ecology. Blackwell Scientific Publication, Oxford, England.
7. Coyne MS. (2001). Soil Microbiology: An Exploratory Approach. Delmar Thomson Learning.
8. Lynch JM & Hobbie JE. (1988). Microorganisms in Action: Concepts & Application in
Microbial Ecology. Blackwell Scientific Publication, U.K.
nd
9. Martin A. (1977). An Introduction to Soil Microbiology. 2 edition. John Wiley & Sons Inc.
New York & London.
10. Stolp H. (1988). Microbial Ecology: Organisms Habitats Activities. Cambridge University
Press, Cambridge, England.
11. Subba Rao NS. (1999). Soil Microbiology. 4th edition. Oxford & IBH Publishing Co. New Delhi.
12. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th
edition. McGraw Hill Higher Education.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-10: RECOMBINANT DNA TECHNOLOGY (THEORY)
SEMESTER –IV
TOTAL HOURS: 60 CREDITS: 4
Unit 5 Construction and Screening of Genomic and cDNA libraries No. of Hours: 6
Genomic and cDNA libraries: Preparation and uses, Screening of libraries: Colony hybridization
and colony PCR, Chromosome walking and chromosome jumping
SUGGESTED READING
1. Brown TA. (2010). Gene Cloning and DNA Analysis. 6th edition. Blackwell Publishing,
Oxford, U.K.
2. Clark DP and Pazdernik NJ. (2009). Biotechnology: Applying the Genetic Revolution.
Elsevier Academic Press, USA
3. Primrose SB and Twyman RM. (2006). Principles of Gene Manipulation and Genomics, 7th
edition. Blackwell Publishing, Oxford, U.K.
4. Sambrook J and Russell D. (2001). Molecular Cloning-A Laboratory Manual. 3rd edition.
Cold Spring Harbor Laboratory Press
5. Wiley JM, Sherwood LM and Woolverton CJ. (2008). Prescott, Harley and Klein’s
Microbiology. McGraw Hill Higher Education
6. Brown TA. (2007). Genomes-3. Garland Science Publishers
7. Primrose SB and Twyman RM. (2008). Genomics: Applications in human biology.
Blackwell Publishing, Oxford, U.K.
B.Sc (HONOURS) MICROBIOLOGY (CBCS
STRUCTURE) C-11: FOOD AND DAIRY
MICROBIOLOGY (THEORY) SEMESTER –V
TOTAL HOURS: 60 CREDITS: 4
Unit 5 Food borne diseases (causative agents, foods involved, symptoms and preventive
measures) No. of Hours: 10
Food intoxications: Staphylococcus aureus, Clostridium botulinum and mycotoxins;
Food infections: Bacillus cereus, Vibrio parahaemolyticus, Escherichia coli, Salmonellosis,
Shigellosis, Yersinia enterocolitica, Listeria monocytogenes and Campylobacter jejuni
Unit 7 Cultural and rapid detection methods of food borne pathogens in foods and introduction
to predictive microbiology. No. of Hours: 5
SUGGESTED READINGS
1. Adams MR and Moss MO. (1995). Food Microbiology. 4th edition, New Age International
(P) Limited Publishers, New Delhi, India.
2. Banwart JM. (1987). Basic Food Microbiology. 1st edition. CBS Publishers and
Distributors, Delhi, India.
3. Davidson PM and Brannen AL. (1993). Antimicrobials in Foods. Marcel Dekker, New York.
4. Dillion VM and Board RG. (1996). Natural Antimicrobial Systems and Food Preservation.
CAB International, Wallingford, Oxon.
5. Frazier WC and Westhoff DC. (1992). Food Microbiology. 3rd edition. Tata McGraw-
Hill Publishing Company Ltd, New Delhi, India.
6. Gould GW. (1995). New Methods of Food Preservation. Blackie Academic and
Professional, London.
th
7. Jay JM, Loessner MJ and Golden DA. (2005). Modern Food Microbiology. 7 edition,
CBS Publishers and Distributors, Delhi, India.
8. Lund BM, Baird Parker AC, and Gould GW. (2000). The Microbiological Safety and Quality
of Foods. Vol. 1-2, ASPEN Publication, Gaithersberg, MD.
th
9. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An Introduction. 9 edition.
Pearson Education.
B.Sc (HONOURS) MICROBIOLOGY (CBCS
STRUCTURE) C-12: INDUSTRIAL MICROBIOLOGY
(THEORY) SEMESTER –V
TOTAL HOURS: 60 CREDITS: 4
Wine, beer
SUGGESTED READINGS
1. Abbas AK, Lichtman AH, Pillai S. (2007). Cellular and Molecular Immunology. 6th
edition Saunders Publication, Philadelphia.
2. Delves P, Martin S, Burton D, Roitt IM. (2006). Roitt’s Essential Immunology.11th edition
Wiley-Blackwell Scientific Publication, Oxford.
3. Goldsby RA, Kindt TJ, Osborne BA. (2007). Kuby’s Immunology. 6th edition W.H. Freeman
and Company, New York.
4. Murphy K, Travers P, Walport M. (2008). Janeway’s Immunobiology. 7th edition Garland
Science Publishers, New York.
5. Peakman M, and Vergani D. (2009). Basic and Clinical Immunology. 2nd edition
Churchill Livingstone Publishers, Edinberg.
6. Richard C and Geiffrey S. (2009). Immunology. 6th edition. Wiley Blackwell Publication.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
C-14: MEDICAL MICROBIOLOGY (THEORY) SEMESTER
–VI
TOTAL HOURS: 60 CREDITS: 4
Unit 1 Normal microflora of the human body and host pathogen interaction
No. of Hours: 8
Normal microflora of the human body: Importance of normal microflora, normal microflora of
skin, throat, gastrointestinal tract, urogenital tract
Host pathogen interaction: Definitions - Infection, Invasion, Pathogen, Pathogenicity, Virulence,
Toxigenicity, Carriers and their types, Opportunistic infections, Nosocomial infections.
Transmission of infection, Pathophysiologic effects of LPS
Unit 7 Antimicrobial agents: General characteristics and mode of action No. of Hours: 8
Antibacterial agents: Five modes of action with one example each: Inhibitor of nucleic acid synthesis;
Inhibitor of cell wall synthesis; Inhibitor of cell membrane function; Inhibitor of protein synthesis;
Inhibitor of metabolism
Antifungal agents: Mechanism of action of Amphotericin B, Griseofulvin
Antiviral agents: Mechanism of action of Amantadine, Acyclovir,
Azidothymidine Antibiotic resistance, MDR, XDR, MRSA, NDM-1
C-14: MEDICAL MICROBIOLOGY (PRACTICAL)
SEMESTER –VI
TOTAL HOURS: 60 CREDITS: 2
1. Identify bacteria (any three of E. coli, Salmonella, Pseudomonas, Staphylococcus, Bacillus)
using laboratory strains on the basis of cultural, morphological and biochemical characteristics: IMViC,
TSI, nitrate reduction, urease production and catalase tests
2. Study of composition and use of important differential media for identification of bacteria:
EMB Agar, McConkey agar, Mannitol salt agar, Deoxycholate citrate agar, TCBS
3. Study of bacterial flora of skin by swab method
4. Perform antibacterial sensitivity by Kirby-Bauer method
5. Determination of minimal inhibitory concentration (MIC) of an antibiotic.
6. Study symptoms of the diseases with the help of photographs: Polio, anthrax, herpes, chicken
pox, HPV warts, AIDS (candidiasis), dermatomycoses (ring worms)
7. Study of various stages of malarial parasite in RBCs using permanent mounts.
SUGGESTED READING
1. Ananthanarayan R. and Paniker C.K.J. (2009) Textbook of Microbiology. 8th edition,
University Press Publication
2. Brooks G.F., Carroll K.C., Butel J.S., Morse S.A. and Mietzner, T.A. (2013) Jawetz, Melnick
and Adelberg’s Medical Microbiology. 26th edition. McGraw Hill Publication
3. Goering R., Dockrell H., Zuckerman M. and Wakelin D. (2007) Mims’ Medical Microbiology.
4th edition. Elsevier
4. Willey JM, Sherwood LM, and Woolverton CJ. (2013) Prescott, Harley and Klein’s
Microbiology. 9th edition. McGraw Hill Higher Education
5. Madigan MT, Martinko JM, Dunlap PV and Clark DP. (2014). Brock Biology of Microorganisms.
14th edition. Pearson International Edition.
B.Sc (HONOURS) MICROBIOLOGY (CBCS
STRUCTURE) DSE-1: BIOINFORMATICS (THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Saxena Sanjay (2003) A First Course in Computers, Vikas Publishing House
2. Pradeep and Sinha Preeti (2007) Foundations of Computing, 4th ed., BPB Publications
3. Lesk M.A.(2008) Introduction to Bioinformatics . Oxford Publication, 3rd International
Student Edition
4. Rastogi S.C., Mendiratta N. and Rastogi P. (2007) Bioinformatics: methods and
applications, genomics, proteomics and drug discovery, 2nd ed. Prentice Hall India Publication
5. Primrose and Twyman (2003) Principles of Genome Analysis & Genomics. Blackwell
B.Sc (HONOURS) MICROBIOLOGY (CBCS
STRUCTURE) DSE-2: MICROBIAL BIOTECHNOLOGY
(THEORY) SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
Unit 6 RNAi No. of Hours: 6 RNAi and its applications in silencing genes, drug resistance,
therapeutics and host pathogen interactions
SUGGESTED READING
1. Fraser CM, Read TD and Nelson KE. Microbial Genomes, 2004, Humana Press
2. Miller RV and Day MJ. Microbial Evolution- Gene establishment, survival and exchange,
2004, ASM Press
3. Bull AT. Microbial Diversity and Bioprospecting, 2004, ASM Press
4. Sangdun C. Introduction to Systems Biology, 2007, Humana Press
5. Klipp E, Liebermeister W. Systems Biology – A Textbook, 2009, Wiley –VCH Verlag
6. Caetano-Anolles G. Evolutionary Genomics and Systems Biology, 2010, John Wiley and Sons
7. Madigan MT, Martink JM, Dunlap PV and Clark DP (2014) Brook’s Biology of Microorganisms, 14th
edition, Pearson-Bejamin Cummings
8. Wilson BA, Salyers AA Whitt DD and Winkler ME (2011)Bacterial Pathogenesis- A molecular
Approach, 3rd edition, ASM Press,
9. Bouarab K, Brisson and Daayf F (2009) Molecular Plant-Microbe interaction CAB International
10. Voit EO (2012) A First Course in Systems Biology, Ist edition,Garland Science
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-4: PLANT PATHOLOGY (THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READINGS
1. Agrios GN. (2006). Plant Pathology. 5th edition. Academic press, San Diego,
2. Lucas JA. (1998). Plant Pathology and Plant Pathogens. 3rd edition. Blackwell Science, Oxford.
3. Mehrotra RS. (1994). Plant Pathology. Tata McGraw-Hill Limited.
4. Rangaswami G. (2005). Diseases of Crop Plants in India. 4th edition. Prentice Hall of India
Pvt. Ltd., New Delhi.
5. Singh RS. (1998). Plant Diseases Management. 7th edition. Oxford & IBH, New Delhi.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-5: BIOMATHEMATICS AND BIOSTATISTICS
(THEORY) SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
Sets. Functions and their graphs : polynomial, sine, cosine, exponential and logarithmic functions.
Motivation and illustration for these functions through projectile motion, simple pendulum,
biological rhythms, cell division, muscular fibres etc.
Simple observations about these functions like increasing, decreasing and, periodicity.
Sequences to be introduced through the examples arising in Science beginning with finite
sequences, followed by concepts of recursion and difference equations. For instance, the Fibonacci
sequence arising from branching habit of trees and breeding habit of rabbits. Intuitive idea of
algebraic relationships and convergence.
Infinite Geometric Series. Series formulas for ex, log (1+x), sin x, cos x. Step function. Intuitive
idea of discontinuity, continuity and limits.
Differentiation. Conception to be motivated through simple concrete examples as given above
from Biological and Physical Sciences. Use of methods of differentiation like Chain rule, Product
rule and Quotient rule. Second order derivatives of above functions. Integration as reverse process
of differentiation.
Integrals of the functions introduced above. Differential Equations of first order, Linear
Differential Equations.
Points in plane and space and coordinate form. Examples of matrices arising in Biological
Sciences and Biological networks. Sum and Produce of matrices upto order 3.
SUGGESTED READINGS
1. H. S. Bear: Understanding Calculus, John Wiley and Sons (Second Edition); 2003.
2. E. Batschelet : Introduction to Mathematics for Life Scientists,Springer Verlag,
International Student Edition, Narosa Publishing House, New Delhi (1971, 1975)
3. A. Edmondson and D. Druce : Advanced Biology Statistics, Oxford University Press; 1996.
4. W. Danial : Biostatistics : A foundation for Analysis in Health Sciences, John Wiley and Sons
Inc; 2004.
B.Sc (HONOURS) MICROBIOLOGY (CBCS
STRUCTURE) DSE-6: INHERITANCE BIOLOGY
(THEORY) SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Gardner EJ, Simmons MJ, Snustad DP (2008). Principles of Genetics. 8th Ed. Wiley-India
2. Snustad DP, Simmons MJ (2011). Principles of Genetics. 6th Ed. John Wiley and Sons Inc.
3. Weaver RF, Hedrick PW (1997). Genetics. 3rd Ed. McGraw-Hill Education
4. Klug WS, Cummings MR, Spencer CA, Palladino M (2012). Concepts of Genetics. 10th
Ed. Benjamin Cummings
5. Griffith AJF, Wessler SR, Lewontin RC, Carroll SB. (2007). Introduction to Genetic Analysis.
9th Ed. W.H.Freeman and Co., New York
6. Hartl DL, Jones EW (2009). Genetics: Analysis of Genes and Genomes. 7th Ed, Jones and
Bartlett Publishers
7. Russell PJ. (2009). i Genetics - A Molecular Approach. 3rd Ed, Benjamin Cummings
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
DSE-7: MICROBES IN SUSTAINABLE AGRICULTURE AND DEVELOPMENT
(THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READINGS
1. Agrios GN. (2006). Plant Pathology. 5th edition. Academic press, San Diego,
2. Singh RS. (1998). Plant Diseases Management. 7th edition. Oxford & IBH, New Delhi.
th
3. Glick BR, Pasternak JJ, and Patten CL (2010) Molecular Biotechnology 4 edition,
ASM Press,
4. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications.
4th edition. Benjamin/Cummings Science Publishing, USA
5. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2nd
edition, Academic Press
6. Barton LL & Northup DE (2011). Microbial Ecology. 1st edition, Wiley Blackwell, USA
7. Campbell RE. (1983). Microbial Ecology. Blackwell Scientific Publication,
Oxford, England.
8. Coyne MS. (2001). Soil Microbiology: An Exploratory Approach. Delmar
Thomson Learning.
9. Altman A (1998). Agriculture Biotechnology, Ist edition, Marcel decker Inc.
10. Mahendra K. Rai (2005). Hand Book of Microbial Biofertilizers, The Haworth Press,
Inc. New York.
11. Reddy, S.M. et. al. (2002). Bioinoculants for Sustainable Agriculture and Forestry,
Scientific Publishers.
12. Saleem F and Shakoori AR (2012) Development of Bioinsecticide, Lap Lambert
Academic Publishing GmbH KG
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE) DSE-8:
BIOSAFETY AND INTELLECTUAL PROPERTY RIGHTS (THEORY)
SEMESTER –V/VI
TOTAL HOURS: 60 CREDITS: 4
Unit 1 No of Hours: 8
Biosafety: Introduction; biosafety issues in biotechnology; Biological Safety Cabinets & their
types; Primary Containment for Biohazards; Biosafety Levels of Specific Microorganisms
Unit 2 No of Hours: 12
Biosafety Guidelines: Biosafety guidelines and regulations (National and International);
GMOs/LMOs- Concerns and Challenges; Role of Institutional Biosafety Committees (IBSC),
RCGM, GEAC etc. for GMO applications in food and agriculture; Environmental release of GMOs;
Risk Analysis; Risk Assessment; Risk management and communication; Overview of International
Agreements - Cartagena Protocol.
Unit 4 No of Hours: 12
Introduction to Intellectual Property: Patents, Types, Trademarks, Copyright & Related Rights,
Industrial Design and Rights, Traditional Knowledge, Geographical Indications- importance of IPR
– patentable and non patentables – patenting life – legal protection of biotechnological inventions –
World Intellectual Property Rights Organization (WIPO).
Unit 5 No of Hours: 12
Grant of Patent and Patenting Authorities: Types of patent applications: Ordinary, PCT,
Conventional, Divisional and Patent of Addition; An introduction to Patent Filing Procedures;
Patent licensing and agreement; Patent infringement- meaning, scope, litigation, case studies, Rights
and Duties of patent owner.
Unit 6 No of Hours: 12
Agreements and Treaties: GATT, TRIPS Agreements; Role of Madrid Agreement; Hague
Agreement; WIPO Treaties; Budapest Treaty on international recognition of the deposit of
microorganisms; UPOV & Brene conventions; Patent Co-operation Treaty (PCT); Indian Patent Act
1970 & recent amendments.
Principles and applications of paper chromatography (including Descending and 2-D), Thin layer
chromatography. Column packing and fraction collection. Gel filtration chromatography, ion-
exchange chromatography and affinity chromatography, GLC, HPLC.
Principle and applications of native polyacrylamide gel electrophoresis, SDS- polyacrylamide gel
electrophoresis, 2D gel electrophoresis, Isoelectric focusing, Zymogram preparation and Agarose gel
electrophoresis.
Principle and use of study of absorption spectra of biomolecules. Analysis of biomolecules using
UV and visible range. Colorimetry and turbidometry.
Preparative and analytical centrifugation, fixed angle and swinging bucket rotors. RCF and
sedimentation coefficient, differential centrifugation, density gradient centrifugation and
ultracentrifugation.
1. Wilson K and Walker J. (2010). Principles and Techniques of Biochemistry and Molecular
th
Biology. 7 Ed., Cambridge University Press.
th
2. Nelson DL and Cox MM. (2008). Lehninger Principles of Biochemistry, 5 Ed., W.H.
Freeman and Company.
3. Willey MJ, Sherwood LM & Woolverton C J. (2013). Prescott, Harley and Klein’s
th
Microbiology. 9 Ed., McGraw Hill.
4. Karp G. (2010) Cell and Molecular Biology: Concepts and Experiments. 6th edition. John
Wiley & Sons. Inc.
5. De Robertis EDP and De Robertis EMF. (2006). Cell and Molecular Biology. 8th edition.
Lipincott Williams and Wilkins, Philadelphia.
th
6. Cooper G.M. and Hausman R.E. (2009). The Cell: A Molecular Approach. 5 Edition.
ASM Press & Sunderland, Washington D.C., Sinauer Associates, MA.
7. Nigam A and Ayyagari A. 2007. Lab Manual in Biochemistry, Immunology and
Biotechnology. Tata McGraw Hill.
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE) SE-1:
Microbial Quality Control in Food and Pharmaceutical Industries
SEMESTER – IV
TOTAL HOURS: 30 CREDITS: 2
Unit 4 HACCP for Food Safety and Microbial Standards No. of Hours: 4
Hazard analysis of critical control point (HACCP) - Principles, flow diagrams, limitations
Microbial Standards for Different Foods and Water – BIS standards for common foods and
drinking water
SUGGESTED READING
1. Harrigan WF (1998) Laboratory Methods in Food Microbiology, 3rd ed. Academic Press
2. Garg N, Garg KL and Mukerji KG (2010) Laboratory Manual of Food Microbiology I
K International Publishing House Pvt. Ltd.
3. Jay JM, Loessner MJ, Golden DA (2005) Modern Food Microbiology, 7th edition. Springer
4. Baird RM, Hodges NA and Denyer SP (2005) Handbook of Microbiological Quality control
in Pharmaceutical and Medical Devices, Taylor and Francis Inc.
B.Sc (HONOURS) MICROBIOLOGY (CBCS
STRUCTURE) SE-2: MICROBIAL DIAGNOSIS IN
HEALTH CLINICS SEMESTER – IV
TOTAL HOURS: 30 CREDITS: 2
SUGGESTED READING
Suggested Readings
Suggested Readings
1. Hui YH, Meunier-Goddik L, Josephsen J, Nip WK, Stanfield PS (2004) Handbook of
food and fermentation technology, CRC Press
2. Holzapfel W (2014) Advances in Fermented Foods and Beverages, Woodhead Publishing.
3. Yadav JS, Grover, S and Batish VK (1993) A comprehensive dairy microbiology, Metropolitan
4. Jay JM, Loessner MJ, Golden DA (2005) Modern Food Microbiology, 7th edition. Springer
B.Sc (HONOURS) MICROBIOLOGY (CBCS STRUCTURE)
SE-5: MANAGEMENT OF HUMAN MICROBIAL DISEASES
SEMESTER – IV
TOTAL HOURS: 30 CREDITS: 2
Suggested Readings
Bioaerosols, Air borne microorganisms (bacteria, Viruses, fungi) and their impact on human health
and environment, significance in food and pharma industries and operation theatres, allergens
Suggested Reading
1. da Silva N, Taniwaki MH, Junqueira VC, Silveira N, Nascimento MS, Gomes RAR (2012)
Microbiological Examination Methods of Food and WaterA Laboratory Manual, CRC Press
2. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications.
th
4 edition. Benjamin/Cummings Science Publishing, USA
nd
3. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2
edition, Academic Press
4. Hurst CJ, Crawford RL, Garland JL, Lipson DA (2007) Manual of
rd
Environmental Microbiology, 3 edition, ASM press
UNIVERSITY OF CALCUTTA
F
O
R
MICROBIOLOGY
2018
Semester Wise Microbiology General Courses
Semester-I
Core Course
B.Sc (General) MICROBIOLOGY (CBCS STRUCTURE)
GE-1: INTRODUCTION AND SCOPE OF MICROBIOLOGY
(THEORY) SEMESTER –I
TOTAL HOURS: 60 CREDITS: 4
Medical microbiology and immunology: List of important human diseases and their causative
agents of various human systems. Definitions of immunity (active/passive), primary and
secondary immune response, antigen, antibody and their types
Environmental microbiology: Definitions and examples of important microbial interactions –
mutualism, commensalism, parasitism, Definitions and microorganisms used as biopesticides,
biofertilizers, in biodegradation, biodeterioration and bioremediation (e.g. hydrocarbons in oil spills)
SUGGESTED READING
1. Tortora GJ, Funke BR and Case CL. (2008). Microbiology: An Introduction. 9th edition.
Pearson Education
2. Madigan MT, Martinko JM, Dunlap PV and Clark DP. (2014). Brock Biology of Microorganisms.
14th edition. Pearson International Edition
3. Cappucino J and Sherman N. (2010). Microbiology: A Laboratory Manual. 9th edition.
Pearson Education Limited
th
4. Wiley JM, Sherwood LM and Woolverton CJ. (2013) Prescott’s Microbiology. 9 Edition.
McGraw Hill International.
5. Atlas RM. (1997). Principles of Microbiology. 2nd edition. WM.T.Brown Publishers.
6. Pelczar MJ, Chan ECS and Krieg NR. (1993). Microbiology. 5th edition. McGraw Hill
Book Company.
7. Stanier RY, Ingraham JL, Wheelis ML, and Painter PR. (2005). General
Microbiology. 5th edition. McMillan.
Semester-II
Core Course
GE-2: BACTERIOLOGY AND VIROLOGY (THEORY)
TOTAL HOURS: 60 CREDITS: 4
Morphological characters: Capsid symmetry and different shapes of viruses with examples
Viral multiplication in the Cell: Lytic and lysogenic cycle
Description of important viruses: salient features of the viruses infecting different hosts -
Bacteriophages (T4 & Lambda); Plant (TMV & Cauliflower Mosaic Virus), Human (HIV &
Hepatitis viruses)
SUGGESTED READING
3. Stanier RY, Ingraham JL, Wheelis ML and Painter PR. (2005). General Microbiology.
5th edition. McMillan
4. Carter J and Saunders V(2007). Virology; principles and Applications. John Wiley and Sons
5. Flint SJ, Enquist, LW, Krug, RM, Racaniello, VR Skalka, AM (2004) Principles of
Virology, Molecular Biology, Pathogenesis and Control.2nd edition.ASM Press
6. Shors Teri (2013) Understanding Viruses 2nd edition Jones and Bartlett Learning Burlington USA
7. Pelczar Jr MJ, Chan ECS, and Krieg NR. (2004). Microbiology. 5th edition Tata McGraw Hill.
th
8. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An Introduction. 9 edition
Pearson Education.
th
9. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9
edition. McGraw Hill Higher Education.
10. Dimmock, NJ, Easton, AL, Leppard, KN (2007). Introduction to Modern Virology. 6th
edition, Blackwell Publishing Ltd.
Unit 1 Microbial Growth and Effect of Environment on Microbial Growth No. of Hours: 12
Definitions of growth, Batch culture, Continuous culture, generation time and specific growth rate
Temperature and temperature ranges of growth
pH and pH ranges of growth
Effect of solute and water activity on growth
Effect of oxygen concentration on growth
Nutritional categories of microorganisms
1. Study and plot the growth curve of E. coli by tubidiometric and standard plate count methods.
2. Calculations of generation time and specific growth rate of bacteria from the graph plotted with
the given data
3. Effect of temperature on growth of E. coli
4. Effect of pH on growth of E. coli
5. Effect of Nitrogen and Carbon sources on E. Coli
6. Effect of salt on growth of E. coli
7. Demonstration of alcoholic fermentation
8. Demonstration of the thermal death time and decimal reduction time of E. coli.
SUGGESTED READINGS
1. Madigan MT, and Martinko JM (2014). Brock Biology of Microorganisms. 14th edition.
Prentice Hall International Inc.
2. Moat AG and Foster JW. (2002). Microbial Physiology. 4th edition. John Wiley & Sons
3. Reddy SR and Reddy SM. (2005). Microbial Physiology. Scientific Publishers India
4. Gottschalk G. (1986). Bacterial Metabolism. 2nd edition. Springer Verlag
5. Stanier RY, Ingrahm JI, Wheelis ML and Painter PR. (1987). General Microbiology. 5th
edition, McMillan Press.
6. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th
edition. McGraw Hill Higher Education.
SEMESTER –III
Skill Enhancement Elective Courses
SE-1: BIOFERTILIZERS AND BIOPESTICIDES
TOTAL HOURS: 30 CREDITS: 2
General account of the microbes used as biofertilizers for various crop plants and their
advantages over chemical fertilizers.
Symbiotic N2 fixers: Rhizobium - Isolation, characteristics, types, inoculum production and
field application, legume/pulses plants
Frankia - Isolation, characteristics, Alder, Casurina plants, non-leguminous crop symbiosis.
Cyanobacteria, Azolla - Isolation, characterization, mass multiplication, Role in rice cultivation,
Crop response, field application.
Suggested Readings
1. Study of different types of DNA and RNA using micrographs and model /
schematic representations
2. Study of semi-conservative replication of DNA through micrographs /
schematic representations
3. Estimation of salmon sperm / calf thymus DNA using colorimeter
(diphenylamine reagent) or UV spectrophotometer (A260 measurement)
4. Resolution and visualization of DNA by Agarose Gel Electrophoresis.
5. Resolution and visualization of proteins by Polyacrylamide Gel Electrophoresis (SDS-PAGE).
6. Study the effect of chemical (HNO2) and physical (UV) mutagens on bacterial cells
7. Study survival curve of bacteria after exposure to ultraviolet (UV) light
8. Demonstration of Bacterial Transformation and calculation of transformation efficiency.
SUGGESTED READINGS
1. Watson JD, Baker TA, Bell SP, Gann A, Levine M and Losick R (2008) Molecular Biology of
the Gene, 6th edition, Cold Spring Harbour Lab. Press, Pearson Publication
2. Becker WM, Kleinsmith LJ, Hardin J and Bertoni GP (2009) The World of the Cell, 7th
edition, Pearson Benjamin Cummings Publishing, San Francisco
3. De Robertis EDP and De Robertis EMF (2006) Cell and Molecular Biology, 8th edition.
Lippincott Williams and Wilkins, Philadelphia
4. Karp G (2010) Cell and Molecular Biology: Concepts and Experiments, 6th edition, John Wiley
& Sons. Inc.
th
5. Sambrook J and Russell DW. (2001). Molecular Cloning: A Laboratory Manual. 4 Edition,
Cold Spring Harbour Laboratory press.
6. Krebs J, Goldstein E, Kilpatrick S (2013). Lewin’s Essential Genes, 3rd Ed., Jones and
Bartlett Learning
7. Gardner EJ, Simmons MJ, Snustad DP (2008). Principles of Genetics. 8th Ed. Wiley-India
8. Klug WS, Cummings MR, Spencer, C, Palladino, M (2011). Concepts of Genetics, 10th
Ed., Benjamin Cummings
9. Maloy SR, Cronan JE and Friefelder D(2004) Microbial Genetics 2nd EDITION., Jones and
Barlett Publishers
10. Russell PJ. (2009). i Genetics- A Molecular Approach. 3rd Ed, Benjamin Cummings
SEMESTER –IV
Skill Enhancement Elective Courses
MICROBIOLOGICAL ANALYSIS OF AIR AND WATER
Bioaerosols, Air borne microorganisms (bacteria, Viruses, fungi) and their impact on human health
and environment, significance in food and pharma industries and operation theatres, allergens
Suggested Reading
1. da Silva N, Taniwaki MH, Junqueira VC, Silveira N, Nascimento MS, Gomes RAR (2012)
Microbiological Examination Methods of Food and WaterA Laboratory Manual, CRC Press
2. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications.
th
4 edition. Benjamin/Cummings Science Publishing, USA
nd
3. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2
edition, Academic Press
4. Hurst CJ, Crawford RL, Garland JL, Lipson DA (2007) Manual of
rd
Environmental Microbiology, 3 edition, ASM press
Semester-V
Elective Course (Any One from DGE-1 and DGE-2)
DGE-1: GENETIC ENGINEERING AND BIOTECHNOLOGY
(THEORY)
TOTAL HOURS: 60 CREDITS: 4
SUGGESTED READING
1. Brown TA. (2010). Gene Cloning and DNA Analysis. 6th edition. Blackwell Publishing,
Oxford, U.K.
2. Clark DP and Pasternik NJ. (2009). Biotechnology: Applying the Genetic Revolution.
Elsevier Academic Press, USA
3. Primrose SB and Twyman RM. (2006). Principles of Gene Manipulation and Genomics, 7th
edition. Blackwell Publishing, Oxford, U.K.
4. Sambrook J and Russell D. (2001). Molecular Cloning-A Laboratory Manual. 3rd edition.
Cold Spring Harbor Laboratory Press
5. Wiley JM, Sherwood LM and Woolverton CJ. (2013). Prescott, Harley and Klein’s
th
Microbiology. 8 edition, McGraw Hill Higher Education
6. Brown TA. (2007). Genomes-3. Garland Science Publishers
7. Primrose SB and Twyman RM. (2008). Genomics: Applications in human biology.
Blackwell Publishing, Oxford, U.K.
DGE-2: MICROBES IN ENVIRONMENT (THEORY)
SEMESTER – V
TOTAL HOURS: 60 CREDITS: 4
1. Analysis of soil - pH, moisture content, water holding capacity, percolation, capillary action.
2. Isolation of microbes (bacteria & fungi) from soil (28ºC & 45ºC ).
3. Isolation of microbes (bacteria & fungi) from rhizosphere and rhizoplane.
4. Assessment of microbiological quality of water.
5. Determination of BOD of waste water sample.
6. Study the presence of microbial activity by detecting (qualitatively) enzymes
(dehydrogenase, amylase, urease) in soil.
7. Isolation of Rhizobium from root nodules.
SUGGESTED READINGS
1. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications. 4th edition. Benjamin/Cummings
Science Publishing, USA
2. Madigan MT, Martinko JM and Parker J. (2014). Brock Biology of Microorganisms. 14th
edition. Pearson/ Benjamin Cummings
3. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2nd edition,
Academic Press
4. Okafor, N (2011). Environmental Microbiology of Aquatic & Waste systems. 1st edition,
Springer, New York
5. Singh A, Kuhad, RC & Ward OP (2009). Advances in Applied Bioremediation. Volume
17, Springer-Verlag, Berlin Hedeilberg
6. Barton LL & Northup DE (2011). Microbial Ecology. 1st edition, Wiley Blackwell, USA Campbell RE.
(1983). Microbial Ecology. Blackwell Scientific Publication, Oxford, England.
7. Coyne MS. (2001). Soil Microbiology: An Exploratory Approach. Delmar Thomson Learning.
8. Lynch JM & Hobbie JE. (1988). Microorganisms in Action: Concepts & Application in
Microbial Ecology. Blackwell Scientific Publication, U.K.
nd
9. Martin A. (1977). An Introduction to Soil Microbiology. 2 edition. John Wiley & Sons Inc.
New York & London.
10. Stolp H. (1988). Microbial Ecology: Organisms Habitats Activities. Cambridge University
Press, Cambridge, England.
11. Subba Rao NS. (1999). Soil Microbiology. 4th edition. Oxford & IBH Publishing Co. New Delhi.
12. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th
edition. McGraw Hill Higher Education.
SEMESTER –V
Unit 4 HACCP for Food Safety and Microbial Standards No. of Hours: 4
Hazard analysis of critical control point (HACCP) - Principles, flow diagrams, limitations
Microbial Standards for Different Foods and Water – BIS standards for common foods
and drinking water
SUGGESTED READING
1. Harrigan WF (1998) Laboratory Methods in Food Microbiology, 3rd ed. Academic Press
2. Garg N, Garg KL and Mukerji KG (2010) Laboratory Manual of Food
Microbiology I K International Publishing House Pvt. Ltd.
3. Jay JM, Loessner MJ, Golden DA (2005) Modern Food Microbiology, 7th edition. Springer
4. Baird RM, Hodges NA and Denyer SP (2005) Handbook of Microbiological
Quality control in Pharmaceutical and Medical Devices, Taylor and Francis Inc.
Semester-VI
Elective Course (Any One from DGE-3 and DGE-4)
DGE-3: MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(THEORY)
TOTAL HOURS: 60 CREDITS: 4
Unit 1 Normal microflora of the human body and host pathogen interaction
No. of Hours: 8
Normal microflora of the human body: Importance of normal microflora, normal microflora of
skin, throat, gastrointestinal tract, urogenital tract
Host pathogen interaction: Definitions - Infection, Invasion, Pathogen, Pathogenicity, Virulence,
Toxigenicity, Carriers and their types, Opportunistic infections, Nosocomial infections.
Transmission of infection,
Unit 7 Antimicrobial agents: General characteristics and mode of action No. of Hours: 7
Antibacterial agents: Five modes of action with one example each: Inhibitor of nucleic acid synthesis;
Inhibitor of cell wall synthesis; Inhibitor of cell membrane function; Inhibitor of protein synthesis;
Inhibitor of metabolism
Antifungal agents: Mechanism of action of Amphotericin B, Griseofulvin
Antiviral agents: Mechanism of action of Amantadine, Acyclovir, Azidothymidine
SUGGESTED READING
1. Ananthanarayan R. and Paniker C.K.J. (2009) Textbook of Microbiology. 8th edition,
University Press Publication
2. Brooks G.F., Carroll K.C., Butel J.S., Morse S.A. and Mietzner, T.A. (2013) Jawetz, Melnick
and Adelberg’s Medical Microbiology. 26th edition. McGraw Hill Publication
3. Goering R., Dockrell H., Zuckerman M. and Wakelin D. (2007) Mims’ Medical Microbiology.
4th edition. Elsevier
4. Willey JM, Sherwood LM, and Woolverton CJ. (2013) Prescott, Harley and Klein’s
Microbiology. 9th edition. McGraw Hill Higher Education
5. Abbas AK, Lichtman AH, Pillai S. (2007). Cellular and Molecular Immunology. 6th
edition Saunders Publication, Philadelphia.
6. Delves P, Martin S, Burton D, Roitt IM. (2006). Roitt’s Essential Immunology.11th edition
Wiley-Blackwell Scientific Publication, Oxford.
7. Goldsby RA, Kindt TJ, Osborne BA. (2007). Kuby’s Immunology. 6th edition W.H. Freeman
and Company, New York.
8. Richard C and Geiffrey S. (2009). Immunology. 6th edition. Wiley Blackwell Publication.
DGE-4: INDUSTRIAL AND FOOD MICROBIOLOGY
(THEORY)
Unit 5 Principles and methods of food preservation and food sanitation No. of Hours: 9
Physical methods - high temperature, low temperature, irradiation, aseptic packaging
Chemical methods - salt, sugar, benzoates, citric acid, ethylene oxide, nitrate and nitrite
Food sanitation and control – HACCP
SUGGESTED READING
1. Crueger W and Crueger A. (2000). Biotechnology: A textbook of Industrial Microbiology.
2nd Edition. Panima Publishing Company, New Delhi
2. Patel AH. (1996). Industrial Microbiology .1st Edition. MacMillan India Limited
Publishing Company Ltd. New Delhi, India
3. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An introduction.9th Edition.
Pearson Education
4. Willey JM, Sherwood LM AND Woolverton CJ (2013), Prescott, Harley and
Klein’s Microbiology.9th Edition. McGraw Hill Higher education
5. Casida LE. (1991). Industrial Microbiology. 1st edition. Wiley Eastern Limited.
6. Stanbury PF, Whitaker A and Hall SJ. (2006). Principles of Fermentation Technology. 2nd
edition, Elsevier Science Ltd.
7. Adams MR and Moss MO. (1995). Food Microbiology. 4th edition, New Age International
(P) Limited Publishers, New Delhi, India.
8. Banwart JM. (1987). Basic Food Microbiology. 1st edition. CBS Publishers and
Distributors, Delhi, India.
9. Frazier WC and Westhoff DC. (1992). Food Microbiology. 3rd edition. Tata McGraw-
Hill Publishing Company Ltd, New Delhi, India.
th
10. Jay JM, Loessner MJ and Golden DA. (2005). Modern Food Microbiology. 7 edition,
CBS Publishers and Distributors, Delhi, India.
SEMESTER –VI
Skill Enhancement Elective Courses
FOOD FERMENTATION TECHNIQUES
Suggested Readings
1. Hui YH, Meunier-Goddik L, Josephsen J, Nip WK, Stanfield PS (2004) Handbook of
food and fermentation technology, CRC Press
2. Holzapfel W (2014) Advances in Fermented Foods and Beverages, Woodhead Publishing.
3. Yadav JS, Grover, S and Batish VK (1993) A comprehensive dairy microbiology, Metropolitan
4. Jay JM, Loessner MJ, Golden DA (2005) Modern Food Microbiology, 7th edition. Springer
Semester Wise Microbiology Honours Courses
1 2 3 4 5 6
Core 2T+2P 2T+2P 3T+3P 3T+3P 2T+2P 2T+2P
Courses (2X4+2X2=12 (2X4+2X2=12 (3X4+3X2=18 (3X4+3X2=18 (2X4+2X2=12 (2X4+2X2=12
Credits) Credits) Credits) Credits) Credits) Credits)
C1: Introduction C3: Biochemistry C5: Virology C8:Microbial C11:Food and Dairy C13:Immunology
to microbiology (Theory+ Practical) (Theory+ Genetics(Theory+ Microbiology (Theory+
and microbial C4: Cell Biology Practical) Practical) (Theory+ Practical) Practical)
diversity (Theory+ Practical) C6: Microbial C9:Environmental C12:Industrial C14:Medical
(Theory physiology and Microbiology Microbiology(Theory Microbiology(The
+Practical) metabolism (Theory+ Practical) + Practical) ory+ Practical)
C2: Bacteriology (Theory+ C10:Recombinant
(Theory Practical) DNA Technology
+Practical) C7:Molecular (Theory+ Practical)
Biology(Theory+
Practical)
Generic 1T+1P 1T+1P 1T+1P 1T+1P
Elective (1X4+1X2=6 (1X4+1X2=6 (1X4+1X2=6 (1X4+1X2=6 Credits)
Courses # Credits) Credits) Credits)
Discipline 2T+2P Any two: 2T+2P Any two:
Specific (2X4+2X2=12 (2X4+2X2=12
Elective Credits) Credits)
Courses DSE-1: Bioinformatics DSE-6: Inheritance
DSE-2: Microbial Biology
Biotechnology DSE-7: Microbes
DSE-3: Advances in in Sustainable
Microbiology Agriculture and
DSE-4: Plant Development
Pathology DSE-8: Biosafety
DSE-5: and Intellectual
Biomathematics Property Rights
and Biostatistics DSE-9:
Instrumentation
and Biotechniques
DSE-10: Project
Work
Ability
Enhance-
ment
Compulsory
Course
Language 1T+0P (2 Credits) 1T+0P (2 Credits)
Course/Env
ironmental
Sc.
Skill 1T+0P 1T+0P
Enhance- (1X2=2 Credits) (1X2=2Credits)
ment Any one Any one
Elective SE-1: Microbial SE-4: Food
Courses Quality Control in Fermentation
Food and Techniques
Pharmaceutical SE-5: Management
Industries of Human Microbial
SE-2: Microbial Diseases
Diagnosis in SE-6:
Health Clinics Microbiological
SE-3: Biofertilizers Analysis of Air and
and Biopesticides Water
Total Credit 20 Credits 20 Credits 26 Credits 26 Credits 24 Credits 24 Credits
140
# Generic Elective Courses can be chosen from Chemistry, Biochemistry, Botany, Zoology, Physiology, Physics, and Mathematics.
Semester Wise Microbiology General Courses
1 2 3 4 5 6
Core Courses* 3T+3P 3T+3P 3T+3P 3T+3P
(3X4+3X2=18 (3X4+3X2=18 (3X4+3X2=18 (3X4+3X2=18
Credits) Credits) Credits) Credits)
GE1§: GE2§: Bacteriology GE3§: GE4§:Microbial
Introduction and and Virology, Biomolecules and Genetics and
Scope of (Theory+ Practical) Microbial Molecular Biology
microbiology, metabolism (Theory+
Bacteriology (Theory Practical)
(Theory +Practical)
+Practical)
Elective 3T+3P 3T+3P
Courses # (3X4+3X2=18 Credits) (3X4+3X2=18
Any one from Credits)
DGE1: Genetic Any one from
Engineering and DGE3: Medical
Biotechnology Microbiology and
DGE2: Microbes in Immunology
Environment DGE4: Industrial
(Theory +Practical) Microbiology and
Food
Microbiology
(Theory+Practical)
Ability
Enhancement
Compulsory
Course
Language 1T+0P (2 Credits) 1T+0P (2 Credits)
Course/Env
ironmental Sc.
Skill Enhance- 1T+0P 1T+0P 1T+0P 1T+0P
ment Elective (1X2=2 Credits) (1X2=2Credits) (1X2=2 Credits) (1X2=2 Credits)
Courses Biofertilizers and Microbiological Microbial Quality Food
Biopesticides Analysis of Air and Control in Food and Fermentation
Water Pharmaceutical Techniques
Industries
Total Credit 20 Credits 20 Credits 20 Credits 20 Credits 20 Credits 20 Credits
120
*For Core Courses- 4 Courses each from 3 disciplines (§GE1, GE2, GE3, GE4 Microbiology and two other disciplines chosen from Chemistry,
Biochemistry, Botany, Zoology, Physiology, Physics, and Mathematics.
# For Elective Courses- 2 Courses each from 3 disciplines (Microbiology and two other disciplines chosen from Chemistry, Biochemistry,
Botany, Zoology, Physiology, Physics, and Mathematics.
Structure of B.Sc. Honours Microbiology under CBCS
1. Total Credits: 140 for Honours
2. Total Marks: 2600
Paper Structure: Theory-50, Practical-50 for Core Courses, Discipline Specific Electives Courses
and Theory-50 for Ability Enhancement Compulsory Courses; Skill Enhancement Elective
Courses, Theory-100, Practical-50 for Generic Elective Courses.
Core Courses: Total Marks: 1400; Total Credits: 84 Credits
Theory- 14 Core Courses X 50=700; 14X4=56 Credits
Practical-14 Core Courses X 50=700; 14X2=28 Credits
Generic Elective Courses: Total Marks: 600; Total Credits: 24 Credits
Theory-4 Courses X 100=400; 4X4=16 Credits
Practical-4 Courses X 50=200; 4X2=8 Credits
Discipline Specific Elective Courses: Total Marks: 400; Total Credits: 24 Credits
Theory-4 CoursesX50=200; 4X4=16 Credits
Practical-4 CoursesX50=200; 4X2=8 Credits
Ability Enhancement Compulsory Courses: Total Marks: 100; Total Credits: 4 Credits
Language Theory Courses-1 CourseX50=50; 1X2=2Credits; Environmental Sciences Theory
Courses-1 CourseX50=50; 1X2=2 Credits
Skill Enhancement Elective Courses: Total Marks: 100; Total Credits: 4 Credits
Theory-2 Courses X 50=100; 2X2=4 Credits