Clin immunology Lecture 2 for dentists
Clin immunology Lecture 2 for dentists
Diseases of the
immune system. Principles of
immunodiagnosis,
immunotherapy, immuno-
reabilitation and
immunoprophylactics.
Lecturer: professor, DM V. Babadzhan.
Immune deficiencies
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 4
Primary Immunodeficiency Diseases
occur when there is a defect
in any one of the many steps
during lymphocyte development
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 5
Classification of Primary IDDs
• Primary B cell immunodeficiency:
X-linked Agammaglobulinaemia (Bruton,s disease)
Selective IgA deficiency
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 7
X-linked Agammaglobulinaemia
(XLA)/Bruton’s Disease:
Deficiency of B cell tyrosine kinase causing failure in
the development of pre-B cell maturation to B cells.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 8
Bacterial Infections
1.Impetigo: This is typified by dermal lesions on face or extremities
and often affects groins and axillae. Lesions, which may be either
bullous (blisters which rupture) or non-bullous (smaller and encrusted)
are highly contagious. The causative agent of bullous lesions is
Staphylococcus aureus and of non-bullous lesions is Group A,
hemolytic streptococcus. Topical or systemic antibiotics are the
customary treatment.
Impetigo:
impetigo
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 10
Selective IgA deficiency (IgA D)
Patients with IgA deficiency have:
IgA levels < 5mg/dL with normal levels of other Igs and
50% have chronic otitis, sinusitis or pneumonia.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 11
Severe Combined Immunodeficiency
Disease (SCID)
Disorder characterized by:
Deficiency in both B and T lymphocyte functions
with markedly low IgG, IgA and IgE levels.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 12
SCID manifests early with:
Persistent and recurrent diarrhoea,
otitis, thrush and respiratory
infections in the first few months of
life.
T cell defects associated with:
Candidiasis, CMV infection, measles and varicella
leading to life threatening pneumonia, meningitis and
sepsis.
SCID managed through Ig infusion, stem cell
transplantation and gene replacement.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 13
T Cell Immunodeficiency Diseases
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 14
Primary T cell immunodefiency
includes:
Di-George syndrome
Wiskott-Aldrich syndrome
Cartilage hair hypoplasi,
Ataxia - telangiectasia
Defective expression of class II MHC
molecules
Defective expression of CD3-T cell receptor
(TCR) complex
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 15
Di George Syndrome (Thymic Aplasia)
Congenital disorder characterized by:
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 16
Ataxia Telangiectasia (AT)
Autosomal recessive progressive
neurodegenerative childhood disorder
associated with:
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 17
Wiskott-Aldrich Syndrome (WAS)
An X-linked recessive disorder associated
with thyrombocytopenia and eczema.
Patients have
Elevated IgA and IgE
Low IgM
Variable T cell dysfunctionsT cell dysfunction
manifested by:
Severe herpex virus and Pneumocystis carinii infections
Increased lymphomas and autoimmune diseases.
Recurrent pyogenic bacterial infections.
Usually affecting ears, sinuses and lungs.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 18
Mixed Immunodeficiencies
Symptoms:
1. Painful generalized ulcers
on oral mucosa.
Sometimes it appears
extra-orally.
2. Low fever and malaise.
Patients often are under-
nourished due to pain
upon eating.
3. Self-limiting. Will last 10
to 14 days.
4. Differential diagnosis
must rule out
candidaisis (thrush)
which is not painful
and produces a white
membranous patches
on mucosal surfaces. Occurs frequently in
young children
VIRAL INFECTIONS
Primary herpes infection:
Treatment:
1. Palliative treatment
Herpangina
chickenpox
Candidosis (Thrush):
Yeast cell
Cell membrane
POLYENES
ergosterol • nystatin
• Amphotercin B
AZOLES
These adsorb to
• miconazole
cell membranes
• fluconazole
and disrupt
• ketoconazole
ergosterols
These inhibit
synthesis of
ergosterols
Recurrent Aphthous Ulcer (canker sore)
AID
S:
Parotid
swelling
thrush
gingivitis
periodo
ntitis Herpetic
ulceration
SIGNS, ALLOWING TO SUSPECT IMMUNODEFICIENCY
Recidivic bacterial-viral infections which are characterized:
a) chronic curse;
b) incomplete reconvalescence;
c) unsteady remissions;
d) unusual microflora (opportunistic infection, with multiresistence stability
to the antibiotics).
Unusual reactions on vaccines.
Information of physical investigation:
delay of development; decline of body mass; subfebril tempereture; increase,
excalation or complete absence of lymphatic knots, amygdales, thymus;
dermatitises, skin abscesses; candidosis of mucous membrane of mouth
cavity.
Haematological changes: leukocitopenia, thrombocitopenia, anaemia.
Paratherapeutic interferences:
chemotherapy; splenectomy; irradiation.
Protracted stress.
Autoimmune diseases.
Tumour.
33
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 33
Acquired immune deficiency is violation of the immune
system, developing in a postneonatal period (in adults)
and not subsequent upon genetic defects.
Acquired immune deficiency is dysimmunity, which
arise up as a result of somatic and other diseases,
and also other factors and have clinical symptoms.
Acquired immune deficiency
a) develops on a background before normally
functioning immune system;
b) characterized the proof decline of quantitative and
functional indexes of immune status;
c) it is the area of risk development of chronic infectious
diseases, autoimmune pathology, allergic diseases and
tumor formations.
(Dranik G.N., 2005)
Forms of acquired (secondary) immunodeficiency
The squared secondary immunodeficiency is a syndrome of
AIDS, developing as a result of defeat of the immune system the
human immunodeficiency virus (HIV).
The inducting (specified) secondary immunodeficiency (ICD-10,
code D.84.8) arises up as a result of concrete reasons, causing
its appearance: x-ray radiation, cytostatic therapy, application of
corticosteroids, traumas and surgical intervention,
dysimmunities, developing the secondary in relation to a basic
disease (diabetes, disease of liver, kinds, malignant tumors).
The spontaneous (unspecified) secondary immunodeficit (ICD-
10, code D.84.9) is characterized absence of reason, causing
violation of immune reactivity. Clinically shows up as chronic,
recidivate infectiously-inflammatory processes of bronchial tree,
additional bosoms of nose, urogenital and gastroenteric system,
35
eyes, skin, soft tissues, caused opportunistic microorganisms.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 35
CLASSIFICATION OF SECONDARY IMMUNODEFICITES
By the rates of development:
Acute immunodeficiency (conditioned an acute infectious disease, trauma,
intoxication and other).
Chronic immunodeficiency (develops on a background of chronic
festering-inflammatory diseases, autoimmunity, tumors, persistent viral
infection).
II. By the level of breakage:
Violation of cellular (Т-cells) immunity.
Violation of humeral (B-cells) immunity.
Violation of phagocytes.
Violation of complement system.
Combined defects.
III. By prevalence:
«Local» immunodeficiency.
Systemic immunodeficiency.
IV. By the degree of severity:
Compensated (mild).
Subcompensated (moderate).
36
Decompensated (severe).
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 36 36
Psychological aspects
beta-endorfins
during exams
lymphopenia
activity of NK cells
production of IFN-gamma
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 37
Secondary Immunodeficiencies
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 38
Secondary Immunodeficiencies
Occur as the result of an underlying
disorder
May be malnutrition, viral infection, cancer,
renal disease and Hodgkin’s disease
May occur with immunosuppressive drugs,
drugs used along with radiation, chemotherapy
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 39
Consequences of immunodeficiencies in
dental patients
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 41
Radiation Therapy (cont.)
Destruction of major salivary glands may result in
xerostomia
The patient is prone to rampant caries and oral
candidiasis.
They also are prone to osteoradionecrosis.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 42
Radiation Therapy (cont.)
Patients should have an oral evaluation
before radiation therapy of the head and
neck.
Potential sources for oral infection and teeth
with a questionable prognosis should be
removed.
The hygienist can help with
Fluoride application
Patient education
Frequent follow-up appointments
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 43
Chemotherapy
Drugs used for cancer chemotherapy
affect basal cells of the epithelium.
Mucositis and oral ulceration are common
complications.
A decrease in all blood cells may occur
Lowered RBC counts can lead to anemia.
Lowered WBC counts can lead to infections.
Lowered platelets can lead to bleeding
problems.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 44
Effects of Drugs on the Oral Cavity
Blood pressure drugs, antianxiety
medications, antipsychotic medications,
and antihistamines can cause xerostomia.
Prednisone suppresses the immune
system and can lead to candidiasis and
oral infections.
Antibiotics may increase risk of
candidiasis.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 45
Effects of Drugs on the Oral Cavity
(cont.)
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 46
Effects of Drugs on the Oral Cavity
(cont.)
Tetracycline can cause tooth discoloration.
Phenytoin and nifedipine can cause
gingival enlargement.
Cyclosporine may cause gingival
enlargement.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 47
Immunotropic preparations
Immunostimulators (immunomodulators)
Biological (human, animal origin):
- Immunoglobulins
- Erbisol
- Thymalin
- Tactivin
Immunosupressive
Glucocorticosteroids
Citostatics
Monoclonal antibodies
Ciclosporin A
48
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 48
Immunoprophilactic and immunoreabilitation
preparations
Vegetable
Bacterial
Mycotic
Antiviral vaccines
Allergovaccintion
49
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 49
Immunostimulators
Clinical criteria: chronic infection, low efficiency of
treatment of inflammatory process; treatment of
cytostatics, glucocorticosteroids, antibacterial and
radial therapy.
Immunological criterias: decrease of amount and
functional activity of lymphocytes, decline level of
immunoglobulines, complement, phagocytic activity
(uncompleted phagocytosis) no less what on 30-
50%.
Immunosupressive therapy
Clinical criteria: heavy forms of allergy with the
defeat of kinds, transplantation of organs and
tissues, connecting system diseases.
Immunological criteria: appearance high 50
titles of
autoantibodyes in blood.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 50
Classification of immune drugs
at the point of application
Affecting primarily Affecting mainly on Affecting influencing
on a cellular link humoral predominantly on
(NK-cells, T-killer (B-lymphocytes, Ig) macrophage- interferon status
cells) monocytic link
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 51
IMMUNOCORRECTION (I)
(ID, lymphocyte type)
CRITERIA
1. Decline of maintenance of CD3, CD4, CD25.
2. Decline of immunoregulatory index of CD4/CD8.
3. Decline of production IL-2, gamma-INF.
4. Increase of production IL-4, 5, 6.
52
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 52
The patient 37 years old. Diagnosis: chronic recurrent herpes viral infection in the area
of the face and lips. Oropharyngeal candidiasis. Immune deficiency in T-
limfotsytopenichnomu type (D 84.9).
Causal and Immunotropic therapy:
1) Zovirax (acyclovir ) 400 mg is
inside the 4 times a day for 1
month ; Gerpevir (ointment )
lubricate the affected
skin and mucous membranes of
the lips 4 times a day 7 days;
3) viferon 500 thousand IU 1 per
day in candlelight for 1 month ;
virohel -
lubricate the affected skin and
mucous membranes of the lips , 2
times a day, 5 - 7 days;
- Interferon inductor - cyclopheron -
12.5 % injection - 2 ml , dose of
0.25 g / m at 1, 2, 4, 6, 8, 11, 14,
17, 20 , 23, 26, 29 days. After
interferon ;
4) imunofan 1 ml / m in a day , №
10;
5) Polyoxidonium 12 mg (
suppository) 1 every 3 days , № 10;
6) amiksin 125 mg (1 cap. ) Day
after breakfast , № 20;
7) intrakonazol ( intrunhar ) 100 mg
1 time a day for 2 weeks .
53
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 53
IMMUNOCORRECION (II)
(ID, interferonone type)
CRITERIA
1. Decline of alfa- and gamma-INF production.
2. Decline of CD4 and CD16 levels .
3. Decline of immunoregulatory index of CD4/CD8.
4. Increase of IL-4, 5, 6 production.
55
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 55
IMMUNOCORRECTION (III)
(ID, humoral type)
CRITERIA
Diminishing of amount of CD19.
Decline levels of immunoglobulins classes A, M, G.
Seronegativ forms of infection (specific IGM-, IGG-, DNA+)
SPECIFIC IMMUNOGLOBULINS:
antistaphylococcal ( 3 ml IM, 3 - 5 inj. 1 time per 3 days ).
antiherpetic (1 or 2 types) (amp. for 1,5 ml, use for 4,5 ml IM
1 time per 3 days of 5 inj.);
anticitomegalovirus;
IG against the virus of Ebshtain-Barr;
antichamidial (1,5 ml one time per 3 days IM of 6 inj.),
56
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 56
The patient 58 years old. Diagnosis: chronic obstructive pulmonary disease, II c.,
Exacerbation. Diffuse fibrosis. Emphysema. LW II c. Immunodeficiency by B-cell type (D
84.9).
Immunotropic therapy:
1) causal antibiotic therapy -
levofloxacin 500 mg / in drip-
Lynn 1 time a day 7 days
dimexyd 5 ml per 200 ml of 0.9%
sodium chloride in / drip 1 per
day 5 days azithromycin 500 mg
1 time a day, 3 days;
2) Polyoxidonium 6 mg / m 2
twice a week, for 10 days;
3) halavit 100 mg 1 time a day /
m, 10 days;
4) laktophiltrum 2 MSA. 2 times
a day for 14 days.
5) fluconazole 100 mg a day, 10
days.
immunorehabilitation:
6) IRS-1 19 inhalations once a
day, 20 days;
7) timalin 1 ml subcutaneously
in a day, 10 days.
57
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 57
INTRAVENOUS IMMUNOGLOBULINS
58
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 58
IMMUNOCORRECION (IV)
(ID, phagocytic type)
CRITERIA
Decline of phagocyte number and index.
Decline of NBT-test.
60
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 60
Vaccination against flu
Autumn (better October)
Possibly simultaneously with other vaccines (in different places)
To utillize the exceptionally registered vaccines
INFLUVAK subunit vaccine
- antigen composition answers annual recommendations of WHO
The first documented case of HIV was traced back to 1959 using
preserved blood samples, which were analyzed in 1998.
1. 1. Advert.org
2. 2. Centers for Disease Control and Prevention
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 62
Definitions
H - Human A - Acquired
I - Immune
I - Immunodeficiency
D - Deficiency
S - Syndrome
V - Virus
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 63
Normal Immune Response
Antibodies
Normal response: Virus invades blood kill virus
Virus
stream and binds to lymphocytes.
Lymphocytes make antibodies to the virus.
Antibodies bind to the virus and destroy
the virus.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 64
Immune Response to HIV
T-cells cannot
produce antibodies
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 65
Immunopathological Mechanisms of HIV
infection
HIV infected patients progress to AIDS disease in three
phases:
Early phase: lasts about 2 weeks accompanied by:
Fever, aches and flue-like symptoms with high levels of virus in
blood.
Middle phase: lasting months or several years (latent) with:
Anti-HIV antibodies
Continuous depletion of CD4 T cells
Late phase (AIDS): characterized by:
Rapid decline in CD4 T cells,
Opportunistic infections including viral (herpes simplex,
herpes varicella zoster, EBV), bacterial (M. tuberculosis),
fungi (Candida-thrush) and protozoan (Microsporidia) .
Cancers (lymphoma; Kaposi’s sarcoma).
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 66
What’s the difference between
HIV and AIDS?
HIV, a virus, eventually causes AIDS,
a syndrome.
Spectrum of HIV
Infection
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 67
T-Cell Count
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 68
Symptoms of HIV
Recurring fever White, thick spots
Night sweats on the tongue
Rapid weight loss Dry cough
Diarrhea lasting Shortness of
several weeks breath
Purple bumps on
the skin, inside the
mouth, and rectum
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 69
Criteria for Diagnosing AIDS
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 70
Opportunistic Infections
Mycobacterium Avium Complex Herpes Simplex
Salmonellosis Herpes Zoster
Syphilis and Neurosyphilis Human Papillomavirus
Tuberculosis Molluscum Contagiosum
Bacillary angiomatosis Oral Hairy Leukoplakia
Aspergillosis Progressive Multifocal
Leukoencephalopathy
Candidiasis
AIDS Dementia Complex
Coccidioidomycosis
Peripheral Neuropathy
Cryptococcal Meningitis
Apthous Ulcers
Histoplasmosis
Malabsorption
Kaposi’s Sarcoma
Depression
Systemic Non-Hodgkin’s
Lymphoma Diarrhea
Primary CNS Lymphoma Thrombocytopenia
Cryptosporidiosis Wasting Syndrome
Isosporiasis Idiopathic Thrombocytopenic
Purpura
Microsporidiosis
Listeriosis
Pneumocystis Carinii
Pneumonia Pelvic Inflammatory Disease
Toxoplasmosis Burkitt’s Lymphoma
Cytomegalovirus Immunoblastic Lymphoma
Hepatitis Valley Fever
Source: AIDS Education Global Information System
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc.
MRSA 71
HIV Infection/AIDS Staging System
Clinical Stages& Major Clinical Features:
Stage 1: Asymptomatic; persistent generalized
lymphodenopathy (PGL) and acute retroviral infection (ARI).
Stage 2: Loss of weight (< 10% of body weight); minor
mucocutaneous infections; herpes zoster and recurrent upper
respiratory tract infections (URTI).
Stage 3: Loss of weight (>10% of body weight); chronic
diarrhoea(> 1 month); prolonged fever; oral candidiasis; oral
hairy leukoplakia; pulmonary tuberculosis; severe bacterial
infections and vulvovaginal candidiasis.
Stage 4: HIV wasting syndrome; extrapulmonary tuberculosis;
Pneumocystis carinii pneumoniae, Candidiasis of the
oesophagus, trachea, bronchi or lungs; toxoplasmosis of the
brain, cryptosporidiasis with mycobacteriosis; lymphoma;
Kaposi’s sacoma (KS) and HIV encephalopathy.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 72
Oral Candidiasis
This is a fungal
infection that occurs
in nearly all patients
with AIDS.
It commonly precedes
other OI’s.
Untreated, it
progresses to the
esophagus and
stomach.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 73
SYSTEMIC DISORDERS WITH ORAL MANIFESTATIONS
AIDS:
AIDS periodontitis
AIDS gingivitis
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. Parotitis 74
Pulmonary Infections:
M. Tuberculosis and Pneumocystis pneumonia common
when CD4 T cells <200/μl.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 75
Gastrointestinal illness:
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 76
Major neurological illnesses:
Toxoplasma encephalitis of the brain caused by
Toxoplasma gondii, progressive multifocal
leukoencephalopathy (PML), demyelinating
disease.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 77
Malignancies:
AIDS-defining malignancies include:
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 78
Diagnosis of HIV
Elisa test
Western Blot test
Oraquick test
Home HIV tests
• Home Access Express
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 79
Fluids that can transmit HIV
Blood Saliva
Semen Tears
Vaginal Fluid Mucus
Breast Milk Urine
(in order of the highest Sweat
concentration of HIV)
Feces
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 80
Universal Precautions
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 81
Barriers include:
Latex Gloves
Latex Dental Dams
Anything that protects your skin from a fluid
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 82
Testing
The test is for antibodies against HIV, not for the
virus itself.
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 83
Timeline
Three-month
window from
first exposure
Three-month
First Second
window from
exposure exposure
second
exposure
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 84
Treatment
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 85
Elsevier items and derived items © 2009 by Saunders, an imprint of Elsevier Inc. 86