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    Santosh Yele
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    Corn Meal Agar is a general purpose medium used for cultivating fungi like Candida albicans and assessing characteristics such as chlamydospore production. The medium contains corn meal infusion and agar, and allows C. albicans to produce chlamydospores which is useful for identification. Tests showed the medium supported growth of C. albicans, Aspergillus brasiliensis, and Saccharomyces cerevisiae and chlamydospore production in C. albicans.

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    Corn meal

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    Corn Meal Agar is a general purpose medium used for cultivating fungi like Candida albicans and assessing characteristics such as chlamydospore production. The medium contains corn meal infusion and agar, and allows C. albicans to produce chlamydospores which is useful for identification. Tests showed the medium supported growth of C. albicans, Aspergillus brasiliensis, and Saccharomyces cerevisiae and chlamydospore production in C. albicans.

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    121 views2 pages

    Corn Meal

    Uploaded by

    Santosh Yele
    Corn Meal Agar is a general purpose medium used for cultivating fungi like Candida albicans and assessing characteristics such as chlamydospore production. The medium contains corn meal infusion and agar, and allows C. albicans to produce chlamydospores which is useful for identification. Tests showed the medium supported growth of C. albicans, Aspergillus brasiliensis, and Saccharomyces cerevisiae and chlamydospore production in C. albicans.

    Copyright:

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    CORN MEAL AGAR

    CAT N: 1164
    For chlamydospore production by Candida albicans and for the culture of phytopatological fungi

    FORMULA IN g/l
    Corn Meal Infusion 2.0 Bacteriological Agar 15.0

    Final pH 6.0 0.2 at 25C

    PREPARATION
    Suspend 17 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121C for 15 minutes. Cool to 50C, mix well and dispense into Petri dishes. The prepared medium should be stored at 8-15C. The color is opaque and white. The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes, discard the medium.

    USES
    CORN MEAL AGAR is a general-purpose medium used for the cultivation of fungi.

    Candida albicans is the etiological agent in Candidiasis, which ranges from a mild to severe skin, nail, and mucous membrane infections. One of the most important differentiating characteristics of C. albicans is its capacity to form
    chlamydospores on some media. Chlamydospore production is an important characteristic for diagnosis used in the identification of C. albicans. Corn Meal infusion provides nitrogen, vitamins, minerals and amino acids essential for growth. Bacteriological agar is the solidifying agent. Corn Meal is valuable for the morphologic differentiation of many yeast-like organisms. It suppresses the vegetative growth of many fungi and at the same time stimulates the sporulation. Corn Meal Agar allows Candida albicans to produce chlamydospores, which is one of the best criterions for identification. Walker and Huppert reported that the addition of 1% Tween 80 enhanced chlamydospore formation.

    MICROBIOLOGICAL TEST
    The following results were obtained of the medium from type cultures after incubation at a temperature of 25 2C and observed after 48-60 hours.
    Microorganisms Growth Good Good Good Chlamydospores + -

    Candida albicans ATCC 10231 Aspergillus brasiliensis ATCC 16404 Saccharomyces cerevisiae ATCC 9763

    BIBLIOGRAPHY
    McGinnis. 1980. Laboratory handbook of medical mycology. Academic Press, New York, N.Y. Walker and Huppert. 1960. Tech. Bull. Reg. Med. Technol. 30:10. Haley and Callaway. 1978. Laboratory methods in medical mycology. HEW Publication No. (CDC) 78-8361. Center for Disease Control, Atlanta, Ga. 1

    LABORATORIOS CONDA, S.A.

    www.condalab.com

    Isenberg (ed.). 1992. Clinical microbiology procedures handbook, vol. 1. American Society for Microbiology, Washington, D.C. Campbell and Stewart. 1980. The medical mycology handbook. John Wiley & Sons, New York,N.Y.

    STORAGE
    Once opened keep powdered medium closed to avoid hydration.
    2C

    25C

    LABORATORIOS CONDA, S.A.

    www.condalab.com

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