Spectrophotometric Determination of Iron Using 1,10-Phenanthroline
Spectrophotometric Determination of Iron Using 1,10-Phenanthroline
Spectrophotometric Determination of Iron Using 1,10-Phenanthroline
Specific Objectives
Obtain absorption spectrum of the tris(1,10phenanthroline)iron(II) complex and determine the analytical
wavelength
Determine percent iron in a solid sample
Absorption of Light
Beer-Lambert Law: A = bc
3
Tris(1,10-phenantholine)iron(II)
max
N
1,10-Phenanthroline
A=bc
= molar absorptivity
b = path length (cm)
c = concentration (mol/L)
Spectrometer
USB cable
Light source
Sample holder
Procedure
Procedure
10 mL of 0.02 M 1,10-phenanthroline
Dilute to volume and measure absorbance at max
Calculations
You want to report %Fe in your solid sample, so you need to find the mass
of Fe in your weighed sample. Here is a general outline of the approach.
Preparation of your stock sample solution.
Your stock sample solution contains all of your solid sample. If you can determine the molar
concentration of Fe in this solution, you can calculate the mass of Fe in your sample.
Prepared standards.
Based on the mass of pure Fe(NH4)2(SO4)2.6H2O, and the dilutions you did, you compute the
concentrations of Fe (mol/L) in your calibration standards.
You measure the absorbance of each standard and construct a calibration curve.
Prepared unknown (the one for which you measured the absorbance)
From the calibration curve, you determine the concentration of Fe (mol/L) in your prepared unknown.
Based on the concentration of Fe in your prepared unknown, and the dilutions(s) of the stock sample
solution you performed to prepare that standard, you compute the molar concentration of Fe in your
stock sample solution.
Now you can calculate the amount of Fe in your unknown sample.
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