LC NMR Lecture
LC NMR Lecture
LC NMR Lecture
Chromatography -
NMR
NMR coupling different kinds of chromatographic
separation systems can be coupled to a flow probe
of an NMR spectrometer.
All Separation techniques like
RP ( Reversed Phase )
NP ( Normal Phase )
GPC ( Gel Permeation Chromatography ) and
SEC ( Size Exclusion Chromatography ) are applicable.
With special equipment also SFC ( Supercritical Fluid
Chromatography ).
LC-NMR experiments can be performed in three modes.
On-flow mode
Direct stopped flow mode
Loop storage mode
On-flow mode:
The outlet of the LC-detector is connected directly to the NMR probe.
While the peaks are eluting, NMR spectra are continuously acquired.
The chromatographic system is used to move the samples/peaks through
the NMR cell.
Ex: Any HPLC system, which delivers a stable pulsation free flow
LC-NMR Probe
LCNMR interface not required
With any of the LC-NMR interface ( BPSU / BSFU ) this working
mode is also possible, however they are not required.
Direct stopped flow mode:
Outlet of the LC-detector is connected directly to the NMR
probe.
A LC-detector ( normally UV ) is used to detect peaks
eluting from the column.
When a peak is detected, the flow continues until the peak
arrives in the NMR cell.
At this time, the chromatography ( pump, data acquisition,
gradient ) stops and the NMR experiments are performed.
Once the NMR experiments are completed, the
chromatography resumes until the next peak is found.
This process can be repeated several times within one
chromatogram.
Equipment:
HPLC under control BRUKER Chromatography software
LC-NMR Probe
BSFU or BPSU-O
Controlling station with BRUKER Chromatography
software
With the LCNMR interfaces BPSU-O/BPSU-36 or
BPSU-12 this working mode is also possible,
however they are not required.
Storage mode
The outlet of the LC-detector is connected to the sample
loops of the BPSU-36 or BPSU-12.
A LC-detector ( normally UV ) is used to detect peaks
eluting from the column.
A detected peak is moved into one of the sample loops
without interrupting the chromatography.
When the chromatography is completed, the HPLC
pump is used to transfer the peaks from the loops into
the NMR probe.
Equipment:
Any HPLC system
pump under control of BRUKER Chromatography
software for transfer
LC-NMR Probe
BPFU-36, BPSU-12
Controlling station with BRUKER Chromatography
software.
LC-NMR interface