Lec 9shigella SPP
Lec 9shigella SPP
Learning Outcomes
flexeneri sonnei
boydii dysenteriae
PATHOGENESIS…
Infect man and other primates.
Infecting dose is small (about 10-100 organisms).
Causes “bacillary dysentery” or “shigellosis”.
Relatively unaffected by gastric acid or bile.
Following ingestion, usually unable to penetrate the
intestine into deeper body tissues or blood.
Primary step in the disease process is the invasion into
the cells of the colonic epithelium by the pathogen.
Produce shigatoxin,protoype of family shigalike toxin.
Cont…
Group D (S.sonnei)
Antigenically homogenous.
K antigens
Fimbrial antigens.
The strains of S.flexeneri produce antigens on
fimbriae which are identical in all the strains
of this group.
Virulence factor…
Three types are considered fundamental to
cause pathology;
Lipopolysaccharide
Exotoxin
verocytotoxin
Habitat…
Contaminated of
food Contaminated of water
Intestinal of human
apes monkeys
SYMPTOMS…
• Abdominal pain • vomitting
Mucous in
blood
stools
tenesmus pus
• diarrhea • fever
Cont…
S.dysenteriae
S.flexneri
S.boydii
S.sonnei
Test Result
Oxidase Negative
Biochemical test…
S.dysenteriae
S.flexneri
S.boydii
S.sonnei
Test result
Motility Negative
Citrate Negative
Urease Negative
Blood agar
Salmonella-
Shigella agar XLD agar
Negative
Urease
Negative VP
Negative
citrate
Negative PD
K/A with no gas Non-motile
Treatment…
Different strains of shigellae differ in their
antibiotic sensitivity to commonly used
agents.
No specific therapy is available.
Treatment with antibiotics tends to prolong
the excretion of bacilli and should be avoided
unless illness is very severe.
References
David B. Frankhauser, 2001.Triple Sugar Iron Agar and Its Use.
University of Cincinnati Clermont College, Batavia. Retrieved on 15
January 2013 from
http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Triple_Suga
r_Iron/TSI_Use.htm
Sridhar Rao P.N. Applied Microbiology cases: Shigella spp.
Department of Microbiology JJM Medical Collleage Davangere,
Karnataka, India. Retrieved on 15 January 2013 from
http://www.microrao.com/about_me.htm
Latish Poulard, 2004. Shigella spp. Missouri S& T Microbiology
Biological Sciences. Retrieved on 15 January 2013 from
http://web.mst.edu/~microbio/BIO221_2004/Shigella.htm
Dr. Nastain Ahmed. Shigella spp. Retrieved on 15 January 2013 from
http://microbiologyreports.hpage.co.in/shigella_spp__40429214.ht
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