Research PPT 101

THE EFFICACY OF MANGOSTEEN
(Garcinia mangostana)
PEEL EXTRACT AS AN ALTERNATIVE
TO EOSIN IN WRIGHT’S STAIN
Sastrellas, Kezia Faith D.

Sidon, Novee Grace J.
Valdes, Francis Zaccheu E.
Background of the Study
 The researchers will conduct this study in order to observe
the effectiveness of mangosteen peel extract dye in
peripheral blood film.
 Mangosteen peels contain pigments from two metabolites,
namely mangosteen and β- mangosteen. Mangosteen
fruit will be extracted and will be obtained natural dye in the
form of anthocyanins, produces red, purple, and blue
colors. It also contain flavan-3,4-diols, classified as tannin.
The result shows that each color intensity from the fixation
process of iron (II) sulfate, alum and lime will be increased
in line with the increasing of the washing time applied.
(Kusumawati N., et al., 2017).
 The major bioactive compounds in Mangosteen are
phenolic acid, prenylatedxanthone derivatives,
anthocyanins, and procyanidins. Major anthocyanin in
mangosteen was cyanidin-3-sophoroside
(Chaovanalikit, et al., 2012).
 The physico-chemical properties: moisture content, ash
content, solids content and pH of Mangosteen peel were
determined by Association of Official Analytical
Chemists (AOAC) method. The chemical compounds in
Mangosteen peel were investigated by phytochemical
tests. (Universities Research Journal 2016)
 Anthocyanin gives Garcinia mangostana purple color
characteristic. The major pigment responsible for the
colouration with dye ranging from orange, pink, red, violet to
blue in the flowers and fruits. The purplish/reddish extract of
this fruit may have a staining effect on a thin blood smear in
clinical laboratory practice that may be used as an
alternative organic stain.
Statement of the Problem
The conduct of this study aimed to examine the the efficacy of
mangosteen peel extract as an alternative stain to Eosin in Wright’s
stain. Specifically, this study answered the following questions:
1. What are the effects of the different concentrations of
mangosteen in staining blood smears in terms of the following
concentrations;
o 15 grams in 100 mL ethanol;
o 20 grams in 100 mL ethanol;
o 25 grams in 100 mL ethanol.
Statement of the Problem
2. What are the effects of the different concentrations

mangosteen peel extract as in terms of:
(a) visual clarity;
(b) cell morphology demonstration;
(c) uniformity of staining.
3. Is there significant difference between the mangosteen

peel extract concentrations with the control, Wright’s stain.
Significance of the Study
 The findings of this study will redound to the benefit of the

society considering that dyes play an important role in
peripheral blood smear staining. The significance of this
study are the following:
 To observe the staining capabilities and efficiency of dye
from the Mangosteen peel in the blood film because natural
dye is easy to extract, non-toxic and have the antimicrobial
properties.
 To the economical rate of mangosteen peel will be increased

because of the demands of this fruit.
 To prove the cost efficiency of the mangosteen peel extract
compared to the commercially available Eosin reagent used
in peripheral blood staining.
 To the Medical Technologist, to avoid or minimize certain
health hazards or risks in utilizing commercial stains.
 To the manufacturer, another source of dye will be presented

to the agriculture department.
 To the future researchers, this research will recommended
for the future studies that will be conducted concerning
peripheral blood staining.
METHODOLOG
Y
Research Flow
Preparation of mangosteen (Garcinia mangostana)
peel stain
Collection of blood sample
Preparation of smear
Staining of blood samples using natural dye available

commercial stains
Staining of blood sample using dye extract stain from

Mangosteen (Garcinia Mangostana)
Comparison of the staining effects
Statistical treatment
Preparation of Materials
 Utilize in this study will obtain from the local market of City
of Koronadal, South Cotabato, Philippines. While in
obtaining blood samples from 15 students of Notre Dame of
Marbel Univeristy, needle and syringe method of
venipuncture were used. The whole experiment was
conducted inside the Notre Dame of Marbel University
Biology and Chemistry Laboratory wherein the staining and
the extraction process will occur.
Preparation of Mangosteen peel stain
 The mangosteen peel will place in a clean plastic container
with sealed lid and stored in room temperature for 24-48
hours before being processed. Extraction and preparation
of the solution were done in line with the study of Chukwu
(2011) and Adegoke et al. (2010) where the mangosteen
peel was washed and dried under the shade for minimum
of 7 days and then milled to form the fine powder.
Preparation of Mangosteen peel stain
 The powder will be stored in glass jars and refrigerated at 4°C
until require for use. Fifteen (15), twenty (20), and twenty-five
(25) grams of the powder was weighed using digital balance,
which was dissolved in 100 mL of ethanol in a beaker and then
filtered using the filter paper. It was transferred to 5mL test tubes,
which were centrifuged at 3000 rpm for 5 minutes and
supernatant were collected in a coupling jar with the use of micro
pipettes.
Staining of Blood Smears
 The blood collected from the 15 respondents must be
anticoagulated preferably with the disodium or dipotassium
salt of ethylene diamine tetra acetic acid (EDTA).
Staining is done by placing 0.05 mL (50 uL) of blood on a
slide and spread lightly to rapidly produce a smear. The
smea The slide was fixed in the methanol for 5 minutes
and dry. Methylene blue is place upon the smear for 2
minutes and then rinse with running water. Eosin Y is
added on the smear for 1 minute. Then, the slide is rinse
again with water and let to dry (Abnova, 2010).
Staining of Blood Smears
All steps are repeated in another slide except that the
mangosteen peel extract is used in exchange of Eosin Y and
for each blood sample. Four smears were made sorted
according to the following groups:
• Group I (Control Group): Stained with Wright’s stain,
• Group II: Stained with 15 grams in 100 mL ethanol
mangosteen extract,
• Group III: Stained with 20 grams in 100 mL ethanol
mangosteen extract, and
• Group IV: Stained with 25 grams in 100 mL ethanol
mangosteen extract.
Comparison of the staining effects
Description
1 Very Poor no observable morphology of cells
2 Poor stained red blood cell only,
morphological alterations present
3 Satisfactory/Average visible stained red blood cells,
platelets with some morphological
alterations
4 Good visibly stained red blood cell,
platelets with no morphological
alterations
5 Very Good visibly stained red blood cells,
platelets and white blood cells with
no morphological alterations
Insights
 The context of this research was to show the staining quality and
capacity of the Mangosteen peel extract as an alternative stain to
Eosin Y in Wright's stain.
 As we were writing this research paper, we had the difficulty in
communicating to each other as we are in our new normal
situation. It is quite difficult to share our ideas through video calls
and chats because of the limited features and as well as the
connectivity to the internet.
 We also had to revise our title from one to another in order to
come up with a good, easy to understand title.
Insights
 In searching for our related literatures, we also had a difficulty as
there were only a few literatures about the mangosteen extract
as an alternative stain in peripheral blood staining. However,
there was a study that concerns about the Mangosteen fruit
extract as an alternative for Histology staining. We incorporated
that study in our research to get recommendations and ideas.
 Despite of all these struggles, we managed to overcome it with
the help of the seminars conducted through online meeting. We
also want to thank our advisers for giving us their time and effort
to help us to make our research paper better.
THANK YOU 

Research PPT 101

Uploaded by

Document Information

Original Description:

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Research PPT 101

Uploaded by

Copyright:

Available Formats

THE EFFICACY OF MANGOSTEEN

Sastrellas, Kezia Faith D.

2. What are the effects of the different concentrations

3. Is there significant difference between the mangosteen

 The findings of this study will redound to the benefit of the

 To the economical rate of mangosteen peel will be increased

 To the manufacturer, another source of dye will be presented

Collection of blood sample

Staining of blood samples using natural dye available

Staining of blood sample using dye extract stain from

Comparison of the staining effects

You might also like