HPLC Column
HPLC Column
(HPLC)
An illustration of an HPLC instrument
Guard column
(Stationary Phase)
Sample
Injection
Port
CH3 CH3
Si—OH + Cl—Si—R → Si—O— Si—R + HCl
CH3 CH3
R – alkyl group
Organo-chlorosilane
(DMCS)
HPLC column
Silica Powder
Porous microparticles
Alumina Powder
SC 3262 HPLC Column 17
Porous-layer or pellicular particles -
consists of spherical glass beads
30-40 µ m diameter covered with porous layer of
adsorbent 1- 3 µ m
Adsorbent may be silica or a collection of small spherical
particles bonded to the larger glass beads or a bonded
organic polymer
Increasing polarity
Hydrocarbon
oxygenated hydrocarbon
proton donors
ionic compounds