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Investigatory Project

Methods
The method section informs the reader of the steps undertaken by
the researcher and how he did them. It includes the following

• All processes done during the actual experiment


• All materials and amounts used in the study
• Description of experimental and control setups
• Kind of data gathered
• Number of trials and replicates done
• Description of the samples and reference population
• Management of sample plants and/or animals
• Sampling technique
• Identification and classification of variables
• Chemical, physical and microbiological analyses of samples
• Manner of data collection, organization and processing
• Statistical analysis (test of significance)
Note:
• The interpretation of the results of the study is taken in the light of
the methods employed. The validity and reliability of the methods
employed enable the reader to put more confidence on the results of
the study.
• Reproducibility of data is a primary consideration in evaluating
reliability of the technique.
• Validity of the methods ensures that the data gathered are relevant
and appropriate to the problem being investigated.
The Method Section
A. Research Design
B. Research Environment
C. Materials and Equipment
D. Experimental Procedures
E. Data Gathering Procedures
F. Statistical Procedures
The use of the Schematic Diagrams
The Gantt Chart
The Research Design
• Is the complete sequential steps the researcher undertakes in order
to achieve the goal of the study
• It is an intelligent plan of the researchers in pursuing the goals of the
study.
• Describes in sufficient detail the procedures employed in the research
so it can be evaluated and repeated if necessary. Such description is
important in evaluating the study. It guides the researcher in the
conduct of the actual experiment.
Basic Principles in Experimental Design
• Replication - It refers to the repetition of the basic experiment done to
provide an estimate variation among observations on units treated alike,
assessing the significance of observed differences. It makes the test of
significance possible.

• Randomization - It is the assignment of the experimental units to the


treatment or vice versa by pure chance. Its purpose is to show that a
factor among various others, can best be considered as the cause of an
observed phenomenon and not other factors. By randomization, the
effects of the extraneous factors that can not be controlled perfectly are
eliminated.
• Control - This refers to the balancing, grouping, and blocking of
experimental units that are employed to the adopted design. In
blocking, experimental units are assigned to a group whose test
organisms show similar characteristics, i.e., age, gender, height,
weight, etc., or test conditions, i.e., pH gradient, salinity, amount of
substance, etc. Its purpose is to achieve homogeneity in the
characteristic among test organisms that are under study.
Common Experimental Design Used
• Pretest Postest of Equivalent Groups
R O1 X O 2
R O1 X O 2

Key:
X = Treatment O1 = Pretest
R = Randomization O2 =Posttest
 
TYPES of Experimental Design
I. COMPLETELY RANDOMIZED DESIGN (CRD)- 
This is used when the experimental units are essentially homogeneous.
Example:
The Effect of Hormones on Weight Gain of Pigs
· Three hormones are available:
- Hormone 1
- Hormone 2
- Hormone 3
· Homogeneity can come in the form of:
- piglets from a litter, if sufficient
- piglets of equal age
- piglets with the equivalent weights
· Suppose 16 experimental pigs were selected:
- four (4) Treatments:
Treatment A : Control
Treatment B : Hormone 1
Treatment C : Hormone 2
Treatment D : Hormone 3
 
· Floor Plan of Pig Pens:
· Experimental Layout: (After Randomization)
II. RANDOMIZED COMPLETE BLOCK DESIGN
(RCBD)

- This design allows experimental units to be meaningfully grouped.

- The number of units in a group is equal to the number of treatments


or multiples of it.

- Blocking allows units to be uniform so that observed differences will


be largely due to treatments.
Collection and Analysis of Data
• Use of Dummy/Empty Tables

Weight of Piglets before the application of treatments


Treatment Replicate 1 Replicate 2 Replicate 2 Replicate 4
Treatment 1
Treatment 2
Treatment 3
Treatment 4
Weight of Pigs 100 days after the application of treatments

Treatment Replicate 1 Replicate 2 Replicate 2 Replicate 4


Treatment 1 47 52 62 51
Treatment 2 50 54 67 57
Treatment 3 57 53 69 57
Treatment 4 54 65 74 59
Treatment Replicate 1 Replicate 2 Replicate 3 Replicate 4 Average
(mean)
Before Treatment 1
Treatment
(pretest) Treatment 2
Treatment 3
Treatment 4
After 100 Treatment 1 47 52 62 51
days
(posttest) Treatment 2 50 54 67 57
Treatment 3 57 53 69 57
Treatment 4 54 65 74 59
After 100 Days After 100 Days
80 80

70

Weight of Pigs
60
Weight of Pigs

60
40

50
20
40
0 t A t B t C t D
en en en en
A B C tD m m m am
n t n t n t n e at eat eat e
e e e e Tr Tr Tr Tr
m tm tm am
e at e a e a e
Tr Tr Tr Tr
Statistical Analysis
• Use Student’s t-test – compare two groups
• Use Analysis of Variance (ANOVA) – compare 3 or more group
Investigatory Project Preparation
Workshop
• Title
• Statement of the Problems of Objectives
• Use Venn Diagram to Outline the Rationale or Background of the
Study and the Significance of Study
• Outline the Review of Related Literature
• Outline Methods using Schematic Diagram
• Cite at least 5 possible References

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