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    Cropprot1 - Botanical Control Approach of Golden Apple Snail

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    Seph Pensotes
    This document describes a study that assessed the molluscicidal activity of two plant species - Hagonoy (Chromolaena odorata) and Asyang (Mikania cordata) - against the golden apple snail (Pomacea canaliculata), a serious rice pest. Plant biomass was soaked in water for 24, 48, or 72 hours to make leachates of different concentrations. Golden apple snails were exposed to these leachates and a control, and mortality was recorded at 24, 48 and 72 hours. The goal was to examine if these locally available plants could serve as alternative botanical pesticides for controlling golden apple snails.

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    Cropprot1 - Botanical Control Approach of Golden Apple Snail

    Uploaded by

    Seph Pensotes
    192 views13 pages
    This document describes a study that assessed the molluscicidal activity of two plant species - Hagonoy (Chromolaena odorata) and Asyang (Mikania cordata) - against the golden apple snail (Pomacea canaliculata), a serious rice pest. Plant biomass was soaked in water for 24, 48, or 72 hours to make leachates of different concentrations. Golden apple snails were exposed to these leachates and a control, and mortality was recorded at 24, 48 and 72 hours. The goal was to examine if these locally available plants could serve as alternative botanical pesticides for controlling golden apple snails.

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    Cropprot1_botanical Control Approach of Golden Apple Snail (2)

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    This document describes a study that assessed the molluscicidal activity of two plant species - Hagonoy (Chromolaena odorata) and Asyang (Mikania cordata) - against the golden apple snail (Pomacea canaliculata), a serious rice pest. Plant biomass was soaked in water for 24, 48, or 72 hours to make leachates of different concentrations. Golden apple snails were exposed to these leachates and a control, and mortality was recorded at 24, 48 and 72 hours. The goal was to examine if these locally available plants could serve as alternative botanical pesticides for controlling golden apple snails.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pptx, pdf, or txt
    192 views13 pages

    Cropprot1 - Botanical Control Approach of Golden Apple Snail

    Uploaded by

    Seph Pensotes
    This document describes a study that assessed the molluscicidal activity of two plant species - Hagonoy (Chromolaena odorata) and Asyang (Mikania cordata) - against the golden apple snail (Pomacea canaliculata), a serious rice pest. Plant biomass was soaked in water for 24, 48, or 72 hours to make leachates of different concentrations. Golden apple snails were exposed to these leachates and a control, and mortality was recorded at 24, 48 and 72 hours. The goal was to examine if these locally available plants could serve as alternative botanical pesticides for controlling golden apple snails.

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    of 13

    BOTANICAL CONTROL APPROACH OF GOLDEN APPLE

    SNAIL, (Pomacea canalicuta Lamarck) USING ASYANG


    ( Mikania Cordata) and Hagonoy ( Chromolaena odorata )

    Kuhol

    Asyang

    Hagonoy
    Golden Apple Snail (GAS)
    Golden apple snail or kuhol was introduced as food to the
    Philippines in the 1980s but turned out to be one of the most
    serious pests of irrigated lowland rice because of its high
    reproductive capacity.

    During a heavy infestation, missing rice hills can be observed in


    the field causing a tremendous reduction in rice yield
    Golden Apple Snail (GAS)
     Chemical control using synthetic molluscicides is the most convenient way for farmers to control golden
    apple snails.

    However, due to increasing awareness of the bad effects of chemical pesticides on humans and
    the environment, there is a need to examine locally available plants as alternative botanical
    pesticides for kuhol.
    Hagonoy ( Chromolaena odorata )

    C. odorata is a very widely distributed tropical SHRUB that is still expanding its
    range, and is considered one of the world’s worst weeds.
    Asyang ( Mikania cordata )
    Objective:
     Assess the molluscicidal activity of Hagonoy (Chromolaena odorata) and asyang (Mikania cordata)
    leachates against golden apple snail

    Materials:
    Pail with cover Plastic Pots Tap Water
    Knife weighing scale Plastic cover enough
    Nylon tulle Asyang biomass to cover plants (so
    that snails can’t
    Record Book Hagonoy biomass escape in the treated
    plants
    Marker (Pentel Pen)
    for labelling
    Procedure:
     A. Collection and Preparation of Treatments
    1. Collection of Hagonoy and Mikania
    Collect about 1 kg of Hagonoy and 1kg Mikania for each treatment to be used. Wash with
    tap water to remove the dirt and other debris. Drain the water for few minutes. Cut into small
    pieces. Soak each cut piece separately in the pail with water (Enough to saturate) for 24, 48, 72
    hours.
    Ex. If the conduct date is Feb 19, 2021, You have to soak 1 kg each of biomass on
    feb 16 (72hrs), another 1 kg of biomass on feb 17(48hrs), and another 1kg of asyang on
    feb 18 (24hrs)
    Be sure to harvest 1,500 ml of leachate per pail

     2. Collection of golden apple snail (kuhol)


    Collect  240 uniform size golden apple snail from untreated ricefield.  Maintain the kuhol in
    tap water before the actual conduct of the experiment. 
    Procedure:
     B. Application of Treatment
     1. Collect and strain the liquid separately
     2. Pour about 500ml per treatment per pot.  Each treatment will be replicated three(3)
    times. Treatments will be  as follows: 
     Group 1 Group 2 

    T0-  Tap Water T0- Tap Water
     T1-Hagonoy soaked for 24 hours T1-Hagonoy soaked for 24 hours
     T1-Hagonoy soaked for 48 hours T2-Hagonoy soaked for 48 hors
     T3-Hagonoy soaked for 72 hours T3-Hagonoy soaked for 72 hours
    R1 R2 R3

    T0=

    T1=

    T2=

    T3=
    Procedure:
     2. Introduce 10 golden kuhol in each pot.
     3. Cover the pot with plastic so that snails cannot escape.
     4. Observe the behavior of kuhol upon introduction 1 hour after treatment
     5. Count the number of dead kuhol in each pot at 24, 48, and 72 hours after treatment.
    Dead Kuhol can be observed as floating with a detached operculum and with the flesh
    out.
     6. Compute the percent snail mortality in all treatments using the formula:
    Procedure:
     7. Analyze your data and make a scientific report.

    Treatments
    24 hrs after 48 hrs after 72 hrs after
    treatment treatment treatment
    R1 R2 R3 R1 R2 R3 R1 R2 R3
    T0
    T1
    T2
    T3
    Thank you

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