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    4 views14 pages

    1.25 - Vitamin C CPAC (+ Calculations Guidance)

    Uploaded by

    expo

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    of 14

    Starter Activity

    What is a free What is an


    radical? antioxidant?
    What do free
    radicals do to the
    human body?
    A free radical is an A molecule that helps
    ionizing molecule e.g. prevent damage by free
    atom with an unpaired e- radicals.
    Free radicals oxidise –
    they damage DNA, Where might you
    Describe the role of proteins etc. obtain antioxidants
    antioxidants in the
    as part of your
    human body. Name some diet?
    examples of
    antioxidants.
    To provide H atoms to From fruit and
    bond to the unpaired e- vegetables, nuts and
    from free radicals. pulses.
    Vitamin C, vitamin E,
    beta-carotene.
    CORE PRACTICAL; Vitamin
    C Content of Juice
    Today’s Lesson Objectives:
    • Carry out the Vitamin C core practical safely, following the method
    provided.
    • Gather accurate and sufficient data on the Vitamin C content of
    different fruit juices.
    • Calculate the Vitamin C content of each fruit juice tested, showing
    full working and units.

    KEYWORDS: • Vitamin C • antioxidant • DCPIP • titration • reduction (gain of electrons) •


    CORE PRACTICAL; Vitamin
    C Content of Juice

    KEYWORDS: • Vitamin C • antioxidant • DCPIP • titration • reduction (gain of electrons) •


    CORE PRACTICAL; Vitamin
    C Content of Juice
    Vitamin C Content of Fruit Juice:

    Which fruit juice


    has the highest
    content of Vitamin
    C?

    KEYWORDS: • Vitamin C • antioxidant • DCPIP • titration • reduction (gain of electrons) •


    CORE PRACTICAL; Vitamin
    C Content of Juice
    Vitamin C Content of Fruit Juice: CPAC

    I will be observing
    carefully to tick off
    the following
    CPAC skills…

    KEYWORDS: • Vitamin C • antioxidant • DCPIP • titration • reduction (gain of electrons) •


    CORE PRACTICAL; Vitamin
    C Content of Juice
    Vitamin C Content of Fruit Juice: CPAC

    KEYWORDS: • Vitamin C • antioxidant • DCPIP • titration • reduction (gain of electrons) •


    CORE PRACTICAL; Vitamin
    C Content of Juice
    Vitamin C Content of Fruit Juice: CPAC

    KEYWORDS: • Vitamin C • antioxidant • DCPIP • titration • reduction (gain of electrons) •


    CORE PRACTICAL; Vitamin
    C Content of Juice
    Vitamin C Content of Fruit Juice: Exemplar Results (2022)

    KEYWORDS: • Vitamin C • antioxidant • DCPIP • titration • reduction (gain of electrons) •


    CORE PRACTICAL; Vitamin
    C Content of Juice
    Vitamin C Content of Fruit Juice: Calculations
    STEP 1: Calculate the mass of Vitamin C needed to decolourise
    the 1cm3 DCPIP…
    Mass of Vitamin C to = 10mg x volume of 1% Vitamin C solution

    decolourise DCPIP
    Example: The average volume of 1% Vitamin C solution needed in
    2022 was 0.25cm3. What was the mass to decolour DCPIP?
    Mass of Vitamin C to = 10mg x 0.25
    decolourise DCPIP
    = 2.5mg of Vitamin C

    https://snabbiology.co.uk/measuring-the-content-of-vitamin-c-in-fruit-juice/
    KEYWORDS: • Vitamin C • antioxidant • DCPIP • titration • reduction (gain of electrons) •
    CORE PRACTICAL; Vitamin
    C Content of Juice
    Vitamin C Content of Fruit Juice: Calculations
    STEP 2: Calculate the mass of Vitamin C in a juice sample…

    Mass of Vitamin C in = Mass of Vitamin C x volume of juice

    juice sample to decolour DCPIP


    Example: The average volume of orange juice needed to decolour
    DCPIP in 2022 was 2.4cm3. What was the mass of Vitamin C in
    this juice?

    Mass of Vitamin C in = 2.5mg x 2.4cm3


    juice sample
    = 6.0 mg/cm3 of Vitamin C

    https://snabbiology.co.uk/measuring-the-content-of-vitamin-c-in-fruit-juice/
    KEYWORDS: • Vitamin C • antioxidant • DCPIP • titration • reduction (gain of electrons) •
    CORE PRACTICAL; Vitamin
    C Content of Juice
    Vitamin C Content of Fruit Juice: Completion of Write-up

    Complete the
    conclusion +
    evaluation
    sections of your
    CPAC write up.

    KEYWORDS: • Vitamin C • antioxidant • DCPIP • titration • reduction (gain of electrons) •


    Plenary Activity – Exam
    Question Practice

    Complete this
    practice exam
    question based
    on the Vitamin C
    core practical.
    Plenary Activity – Exam
    Question Practice
    ANSWERS

    (a)
    Plenary Activity – Exam
    Question Practice
    ANSWERS

    (b)

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